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  • 1
    Electronic Resource
    Electronic Resource
    Vectors for Cu2+-inducible production of glutathione S-transferase-fusion proteins for single-step purification from yeast (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 441-449 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; CUP1 ; Cu2+-regulated ; yeast expression ; affinity purification ; glutathione S-transferase ; metallothionein ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We describe six new yeast episomal vectors which encode glutathione S-transferase (GST) affinity tags. These allow for the production of GST-fusion proteins in Saccharomyces cerevisiae under the control of the CUP1 promoter. Affinity chromatography with glutathione-Sepharose permits convenient purification of the fusion protein from a yeast lysate. The presence of a protease cleavage site facilitates subsequent removal of the GST tag. The expression and single-step purification of both GST and a functional GST-metallothionein fusion from yeast are shown as an example of the application of these vectors.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320100403
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