ISSN:
0749-503X
Keywords:
Kluyveromyces marxianus
;
Kluyveromyces lactis
;
ribosomal protein
;
ABF1 regulation
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The abundant multifuctional protein ABF1 of Saccharomyces cerevisiae binds to the upstream region of several genes, including some ribosomal protein genes like the one encoding protein S33. Deletion of th ABF1-binding sequence lowers the transcription of these genes three- to more than ten-fold.We have isolated the S33 genes of two related yeast species. Kluyveromyces lactis and Kluveromyces marxianus. Comparison of the nucleotide sequences of these S33 genes with their counterpart form S. Cerevisiae shows a strong sequence similarity covering the whole of the coding regions. In contrast, little or no sequence similarly is found in the 5′-flanking regions of the three genes. Also the trailer regions differ considerably in both length and sequence from one species to another.An ABF1-binding site is present in the upstream region of the S33 gene of K. marxianus. Retardation analyses showed that this sequences is able to bind a protein present in Kluyveromyces cells with a molecular mass somewhat lower than that of S. cerevisiae ABF1. Functional analyses, using a β-glucuronidase reporter system, showed that the ABF1-binding site is indeed involved in transcription activation of the K. marxianus S33 gene in Kluyveromyces DNA and Northern blots did not show a signal.These results indicate that S. cerevisiae and Kluyveromyces contain functionally related but structurally dissimilar ABF1-type proteins.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/yea.320081105
