ISSN:
0006-3592
Keywords:
transfectoma
;
chimeric antibody
;
stability
;
Southern blot
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The stability of KR12H-1 transfectoma in regard to chimeric antibody production was examined during long-term, repeated batch culture without selection pressure using antibiotics. Both serum-supplemented and serum-free media were used. Regardless of the medium used, the specific antibody productivity (qAb) of transfectoma decreased by 60% to 88% during 70-day culture. This loss of antibody productivity was not due mainly to the appearance of a nonproducing population (NP) of transfectoma. The percentage of a producing population (P), which was monitored by the limiting dilution method, remained over 90% until the end of culture, indicating that the qAb of P decreased during the culture. Flow cytometric data also showed the increase of cell population with low fluorescence intensity during culture, indicating that the intracellular antibody content of P decreased. The subclones of P obtained at the end of long-term culture were further characterized. Compared with the qAb of P at the beginning of long-term culture, the qAb of most P subclones was significantly low, confirming that the loss of antibody productivity was due mainly to the decreased qAb of P during long-term culture. The decreased antibody gene copy number of P subclones was found to be partly responsible for the decreased qAb of P during long-term culture. © 1996 John Wiley & Sons, Inc.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
