ISSN:
0006-3592
Keywords:
chemotaxis
;
single cell
;
capillary
;
Escherichia coli
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A new method was developed which enables chemotaxis parameters to be measured at a single-cell level inside a capillary for the first time. The chemotaxis chamber consists of two reservoirs communicating through a capillary tube 50 μm in diameter. Chemotaxis parameters are measured inside the capillary using image analysis, after a nearly linear attractant concentration gradient has been generated along the capillary by diffusion. Compared to previously published techniques, this method provides a well-characterized chemoattractant concentration profile in addition to allowing single-cell parameters to be measured inside a fine capillary. This procedure was used to measure the single-cell chemotaxis parameters for Escherichia coli K12, and the results are compared to published data on single E. coli cells chemotaxing in bulk. © 1996 John Wiley & Sons, Inc.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
