ISSN:
0006-3592
Keywords:
cytochrome P-450cam monooxygenase
;
bioaugmenattion
;
halocarbon degradation
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Pseudomonas putida PpG786 that contains the inducible enzyme system cytochrome P-450cam is considered for use as specialized biomass fore detoxification of hazardous hydrocarbons. The test substrate 1,2-dibromochloropropane (DBCP) is used to assess the organohalide degradation activity of P. Putida PpG786. Activity was found to be a strong function of intracellular heme content, variables which affect the culturing and processing of the cells, and oxygen tension in the degradation incubation medium. The lifetime for maintaing active biomass in chemostat washout operation, after including substrate was removed and then restarted, was also studied. These results indicate that initial activity of the P. Putida biomass is high enough, and decays slowly enough, so that industrial wastewater treatment at the operating conditions of a sequencing batch reactor (SBR) could remove hazardous compounds. © 1994 John Wiley & Sons, Inc.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260440214
