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Effect of activation energy microheterogeneity on first-order enzyme deactivation (1987)

Malhotra, Arun ; Sadana, Ajit

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 30 (1987), S. 108-116

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: The influence of microheterogeneity on enzyme inactivation kinetics is examined. A continuous normal distribution of the thermal activation energy is assumed, and using this, a simple mathematical model is developed to find the activity-time trajectories for a microheterogeneous enzyme. Using an example, the model is used to show the quantitative effects of microheterogeneity such as increased order and stability observed during an enzyme inactivation. Experimental measurement of the extent of microheterogeneity in an enzyme sample is also discussed.

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260300116

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