ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A regenerating reaction combined with the use of native malate dehydrogenase, native diaphorase, methylviologen, NAD, oxalacetic acid as the substrate and lipoamide as a stabilizer was carried out in the presence of electrolysis. Consequently, malic acid was efficiently produced from oxalacetic acid in the regenerating reaction. A glassy carbon bead electrode was used as a cathode. Twenty four milliamperes were passed at a rotation speed of 500 rpm, 29.8 ± 0.3°C and -1.0 V. It was found that lipoamide has a stabilizing effect on malate dehydrogenase and diaphorase. Low concentration (50 μM) of NAD was also effective for the stabilization of malate dehydrogenase. NADH regeneration activity based on malic acid production rate was 4.7 U/mg of the enzyme protein of the commercial diaphorase preparation. The current efficiency was more than 74%, compared with the theoretical yield, in the presence of enough oxalacetic acid.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260270508
