ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Comparative CD studies have been carried out to characterize the properties of 2′-5′ and 3′-5′ oligoriboadenylates and oligoribouridylates from dimer to decamer. The CD band of the 3′-5′ oligoribonucleotides was larger than that of the 2′-5′ oligoribonucleotides and increased with the increase in chain length, while the CD band of the 2′-5′ oligoribonucleotides increased little beyond the dimer level. The CD analysis of the chain length dependency revealed that the 3′-5′ oligoribonucleotides adopt mainly the base-base stacking interaction, while the base-sugar interaction is predominant in the 2′-5′ oligoribonucleotides. The CD intensity of 3′-5′ oligoribonucleotides decreased to a larger extent at elevated temperatures or in the presence of ethanol compared to that of the 2′-5′ counterparts. Mg2+ or Mn2+ ion enhanced the magnitude of the CD of 3′-5′ octariboadenylate, while a small decrease in the CD was observed by the presence of Mg2+ or Mn2+ ion to the 2′-5′ octariboadenylate. The 3′-5′ oligoribonucleotide is likely conformationally flexible and can form helical ordered structure with strong base-base stacking depending on changes in the environment such as temperature, the presence of Mg2+ ion, or hydrophobicity of the solution. © 1996 John Wiley & Sons, Inc.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
