ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The diffusion, sedimentation, and viscosity behavior of native DNA has been studied as a function of molecular weight absolutely determined by diffusion and sedimentation measurements between 106 and 20 × 106 Daltons. Only data for monodisperse subfractions of the polydisperse DNA samples investigated have been compared. They were derived by means of calculations based on individual sedimentation constant distribution and polymer parameters. The inconstancy of these parameters for the semi-rigid DNA molecules over the range of different sub-fractions of the polydisperse samples has been taken into account. A detailed description of the corresponding mathematical background as well as of the diffusion measurements has been given in the two preceding papers. For the homologous series of monodisperse native DNA molecules, three-parameter equations have been determined representing the s0-[η], s0-M, and [η]-M relations as well as the D0-s0 and D0-M interdependence over the entire molecular weight range of interest. Furthermore a simple equation is given describing the molecular weight dependence of the Mandelkern-Flory-Scheraga parameter β in terms of s0 and [η]. The asymptotic value β∞ has been determined, primarily by measurements at finite molecular weights, to be 2.39 × 106, resulting in β values near 2.50 × 106 at M = 108 Daltons. The hydrodynamic properties of our two calf thymus DNA samples of highest molecular weight proved to be influenced by residual protein interactions resulting in a more compact conformation. Further data are given demonstrating the influence of the individual polydispersity of the investigated samples on diffusion constant and molecular weight averages.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.360100206
