ISSN:
0001-1541
Keywords:
Chemistry
;
Chemical Engineering
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The diffusion of proteins in polymer matrices is an important step in the adsorptive and chromatographic processes used for protein purification, as it is often rate-limiting. Methods for the estimation of the intraparticle diffusivity in polymer gels have been developed and were applied to the diffusion of seven model proteins in agarose particles. The intraparticle diffusivity was not affected by particle diameter. A correlation based on the restricted diffusion model of Ogston et al. (1973) and Cukier (1984) has been proposed. This correlation allows the estimation of protein diffusivity in these matrices based on the molecular weight of the protein and the polymer concentration.
Additional Material:
13 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/aic.690380212
