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Measurement of urinary somatomedin C by a chemiluminescent method (1989)

Orlando, Claudio ; Tode, Bettina ; Pinzani, Pamela ; [et al.]

New York : Wiley-Blackwell

Journal of Bioluminescence and Chemiluminescence 3 (1989), S. 47-51

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ISSN: 0884-3996

Keywords: Chemiluminescent immunoassay ; somatomedin C ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: We present a method for the measurement of the total Somatomedin C (SmC) content in human early morning urine samples after dialysis, extraction, and concentration. We modified a chemiluminescence immunoassay, previously developed for SmC determination in serum, for analysis of SmC in urine. Appropriate sensitivity was obtained by the preparation of a new chemiluminescent tracer (AEEI-COOH-SmC) and the optimization of a competitive non-equilibrium immunoassay system which had a detection limit of 0.24 fmol SmC per tube.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bio.1170030203

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