ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Bacteriophage T4, bacteriophage T4 without its tail fibers, and bacteriophage P22 without its tail have been subjected to electrophoresis in agarose gels; electrophoretic mobility (μ) of these particles as a function of the concentration of agarose (A) has been determined using preparations of agarose with different amounts of electro-osmosis. When μ was extrapolated to an A of 0 (μo), it was found that a term, μE, constant for each preparation of agarose must be added to μo to correct for electro-osmotic flow. A relationship between μE and a manufacturer-determined coefficient of electro-osmosis has been empirically derived. Corrected μo's are in agreement with data obtained by solid support-free electrophoresis.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150030204
