ISSN:
1040-0397
Keywords:
Peptide nucleic acid
;
Adsorption
;
DNA biosensors
;
Carbon paste electrodes
;
Stripping potentiometry
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Peptide nucleic acids (PNA) are new DNA analogs which offer great promise for highly specific DNA biosensors, for use as antisense drugs, or for various molecuilar biology applications. This article evaluates the interfacial behavior of PNA at carbon paste electrodes, in comparison to DNA. While both PNA oligomers display a strong adsorption onto the carbon surface, they differ intheir interfacial properties due to differences in change and structure. Factors influencing the adsorption behavior, including the adsorption potential or time, PNA concentration, coexisting anions and cations, or buffer concentration, are explored. The strong adsorption is exploited for an effective preconcentration step prior to the chronopotentiometric measurement. The resulting adsorptive stripping potentiometric protocol offers convenient quantitation of ng/mL levels of PNA, as desired for future diagnostic, pharmaceutical and biological applications of these DNA analogs.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140090206
