ISSN:
0935-6304
Keywords:
Capillary electrophoresis
;
CTAB
;
Dipeptides
;
Enkephalins
;
Proteins
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Incorporation of a low concentration of cetyltrimethylammonium bromide (CTAB) in the running electrolyte is shown to dynamically coat the silica capillary and to reverse the direction of electroosmotic flow. The CTAB coating prevented interaction of proteins with the capillary surface and enabled sharp peaks to be obtained in the electropherograms. A systematic study of experimental parameters demonstrated the importance of selecting a suitable buffer electrolyte and an appropriate pH. Excellent separations were obtained for five proteins, three enkephalins, and six dipeptides with an efficiency of approximately 500,000 theoretical plates per meter. The method developed is very simple to perform and was found to give excellent reproducibility.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240201102
