ISSN:
0935-6304
Keywords:
Packed column SFC
;
Selectivity tuning
;
Refsum's syndrome
;
Phytanic acid
;
Human blood serum
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A new packed column SFC method has been developed for the determination of phytanic acid in human blood serum. Because of the low viscosity and high diffusion coefficient of supercritical carbon dioxide, several columns can be connected in series, significantly improving separation. Series connection of columns which differ in their selectivity enables the adjustment of the total selectivity towards the individual separation problem (‘selectivity tuning’). In this instance it was possible, after transesterification into their ethyl esters, to separate all the fatty acids in human blood serum, including phytanic acid, by coupling three columns (silica, amino-propyl, and RP8) in series.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240161205
