ISSN:
0935-6304
Keywords:
Gas chromatography
;
Open tubular column
;
n-Pentane
;
Human breath analysis
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A new procedure, requiring expired sample volumes of approximately 2L, has been developed for the quantitative determination of n-pentane in human breath. The methodology capitalizes on the superior resolving power and separating efficiency of the open tubular capillary column and incorporates an effective prechromatographic cryofocusing technique. Molecular sieve is used to simultaneously remove moisture and carbon dioxide from breath samples which are “spiked” with an internal standard to optimize the validity of analysis. Reproducible data for both internal standard spiking (CV = 5.8%) and sample n-pentane level (CV = 3.2%) have been obtained, together with a mean recovery of 104%. Preliminary data show n-pentane levels in normal female subjects (n = 10) to range from 0.62 to 3.16 nmole/L (mean 1.76 nmole/L) and in males (n = 10) from 3.20 to 8.76 nmole/L (mean 5.66 nmole/L).
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240160712
