ISSN:
0935-6304
Keywords:
Capillary GC
;
Solid phase extraction
;
Cold on-column injection
;
Antiepileptic drugs
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A simple and quick gas chromatographic method has been developed for the determination of up to six commonly used antiepileptic drugs in human serum. The antiepileptics are isolated from serum by solid phase extraction on to a reversed phase sorbent and recovered with ethyl acetate as eluent. The ethyl acetate eluate is suitable for direct on-column injection on to a phenyl methyl siloxane capillary column; hydrogen is used as carrier gas and the compounds are separated with a two-ramp temperature program. Detection is by FID.The reproducibility of the method varies between 2 and 6% RSD, depending on the drug and the level analyzed; limits of detection were found to be 14-51 pg and minimum assayed concentrations in serum were between 14 and 51 ng/ml.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240160105
