ISSN:
0935-6304
Keywords:
Thin layer chromatography, TLC, HPTLC
;
Quantitation
;
Curve fitting not possible by conventional least squares techniques
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Analytical results are often obtained from reflectance or fluorescence measurements in TLC or HPTLC with the aid of calibration lines. Curve fitting may not be done by conventional least squares methods for both the independent and the dependent variables are erroneous; errors occur in the volumes spotted on to the plate and in the reflectance/fluorescence measurements. Therefore the sum of the squares of the distances to the calibration line has to be minimized instead of the squared sum of the errors of the dependent variable. The algorithms used are derived and explained. Implications of error propagation for the analytical result are also given.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240041006
