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    Sirius red-collagen interaction: A method for the measurement of collagen and bacterial collagenase activity (1980)
    Academic Press
    In:  Biochemistry International, 1 (5). pp. 455-462.
    Details
    Publication Date: 2018-11-20
    Description: The dye Sirius Red binds to collagens types I, II and III, giving red-colored products in which the bound dye is proportional to the amount of collagen present. Several other proteins also bind Sirius Red, in amounts 30-50 times less than collagen, on a weight basis. Because of this preferential binding to collagen, Sirius Red may be used for its quantitation even in presence of other proteins, as it occurs, for instance, in the culture medium of various types of cultured cells. When incubated at room temperature with bacterial collagenase, the Sirius Red-collagen complex is rapidly degraded while it is resistant to mammalian collagenase, trypsin and pepsin. Thus, the release of the bound dye may be used for quantitation of bacterial collagenase activity.
    Type: Article , PeerReviewed
    Format: text
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