Publication Date:
2021-07-06
Description:
The original method, proposed by Yentsch (1957), of determination of chlorophyll directly in the cells, attracts attention by its simplicity. In order to measure the content of chlorophyll by this method, a determined volume of suspension of algae is filtered through a membrane filter. The latter is dried a little, clarified by immersion oil, clamped between two glasses, and spectrophotometrized. Extinction is read off at , wavelengths equal to 670 millimicrons (around the maximum absorption of chlorophyll a in the cell) and 750 millimicrons (correction for non- specific absorption and dispersion of light by particles of the preparation). The method of Yentsch was employed by the authors for determination of chlorophyll-a in samples of phytoplankton. They conclude that in spite of the simplicity and convenience of determination the method must be applied sufficiently carefully. It is more suitable for analysis of cultures of algae, where, non-specific absorption of light is insignificant.
Description:
Translated from Russian into English
Keywords:
Biology
;
Ecology
;
Limnology
;
Algae
;
Chlorophylls
;
Methodology
;
Biofilters
;
Light absorption
Repository Name:
AquaDocs
Type:
monograph
Format:
application/pdf
Format:
application/pdf
