Publication Date:
2024-07-01
Description:
Sediment cores were collected using a multicorer (MUC). Microbes and virus-like particles were extracted from sediments in a 3°C room using a modified version of the washing protocol of Danovaro and Middelboe, 2010 (see Schauberger et al., 2021). After the washing procedure, the extracted microbial cells and virus-like particles were fixed with 25% glutaraldehyde (1% final concentration) and stored at −80°C prior to flow cytometry. These samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I. Sediment extracts were diluted 1 : 10 in 0.02 μm-filtered TE Buffer prior to all measurements. The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021).
Keywords:
Abundance; Bacteria; Carbon, organic, total; Date/Time of event; Deep sea; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Elevation of event; Event label; Flow cytometry system, Becton Dickinson, FACSCanto II; GeoB22902-2, Site 6; Hadal trench; Latitude of event; Longitude of event; Microbial abundance, cells; Microbial abundance, standard deviation; MUC; MultiCorer; organic matter; prokaryote; sediment; Site 1; Site 10; Site 2; Site 3; Site 4; Site 5; Site 7; Site 9; SO261; SO261_106-1; SO261_117-1; SO261_22-1; SO261_36-1; SO261_49-1; SO261_64-1; SO261_76-1; SO261_9-1; SO261_93-1; Sonne_2; Station label; Viral abundance; Viral abundance, standard deviation; virus-like particles
Type:
Dataset
Format:
text/tab-separated-values, 2167 data points
