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Detection of koi herpesvirus in common carp, Cyprinus carpio L., by loop-mediated isothermal amplification (2004)

Gunimaladevi, I ; Kono, T ; Venugopal, M N ; [et al.]

Oxford, UK : Blackwell Science Ltd

Journal of fish diseases 27 (2004), S. 0

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ISSN: 1365-2761

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology , Medicine

Notes: Loop-mediated isothermal amplification (LAMP) is a novel method that amplifies DNA with high specificity and rapidity under isothermal conditions. In this study, using the LAMP method, a protocol for koi herpes virus (KHV) detection in common carp was designed. A set of four primers, two inner and two outer, were designed based on the sequence of the thymidine kinase (tk) gene of KHV. Time and temperature conditions for detection of KHV were optimized for 60 min at 65 °C. The detection limit using LAMP was found to be similar to that by polymerase chain reaction. In this study, we have developed a highly sensitive and rapid diagnostic procedure for detection of KHV infection in common carp.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2761.2004.00578.x

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