ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Samples were collected from 3 hatchery populations of 2 trout inbreeding centers (Kelardasht and Jajrood) and mitochondrial DNA (mtDNA) of these samples was studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). ND5/6 segment and a fragment that includes 12SrRNA, tRNA F, P, T, D-loop, and a part of cyt.b of mtDNA were amplified and investigated using different restriction enzymes. Two patterns were observed using HaeIII, AvaII, RsaI, TaqI, and Hinf enzymes on gel electrophoresis, resulting in 9 haplotypes in ND5/6 segment, whereas only 1 pattern was observed for the fragment containing D-loop. As a measure of intrapopulation diversity and interpopulation diversity, both heterozygocity and genetic distance were determined, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2003.tb08258.x