Digitale Medien
s.l. ; Stafa-Zurich, Switzerland
Key engineering materials
Vol. 364-366 (Dec. 2007), p. 1147-1150
ISSN:
1013-9826
Quelle:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Thema:
Maschinenbau
Notizen:
A novel multi-channel DNA fragment analysis system is presented,which is based onsingle PMT confocal fluorescence detection and optical scanning adopting an f-theta lens. Capillaryelectrophoresis experiments were executed in the system for DNA marker pBR322/Hae III. Signalwas processed with wavelet denoising. The system’s limit of detection (dsDNA with the probe ofThiazole Orange, TO) was evaluated to be 1.1841×10-11mol/L. Its working noise is much lowercompared with that based on mechanical scanning one, and the stability and detecting sensitivity ishigh. The system was expected to be applied to both capillary array and microchip electrophoresisdetection based on laser-induced fluorescence
Materialart:
Digitale Medien
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.364-366.1147.pdf
|
Standort |
Signatur |
Erwartet |
Verfügbarkeit |