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HPLC determination of tryptophan enantiomers with photometric, fluorimetric and diode-laser polarimetric detection (1996)

Sánchez, F. García ; Díaz, A. Navas ; Pareja, A. García

Springer

Chromatographia 42 (1996), S. 494-498

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ISSN: 1612-1112

Keywords: Column liquid chromatography ; Tryptophan ; Chiral separations ; Diode-laser polarimetric detection

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary The combined utilization of photometric, fluorimetric and polarimetric detection liquid chromatography detectors in series for the identification and quantification of D-tryptophan and L-tryptophan was evaluated. Detection limits of about 1 μg were established with the range of linearity extending to about 100 μg. The relative standard deviation of the D-form and L-form tryptophan were 7.32 and 4.22%, respectively. The amount unknown of tryptophan enantiomers in the different mixtures was determined with an accuracy of 1.58% at the 40 μg injection level.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02290282

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