ISSN:
1617-4623
Schlagwort(e):
Yeast
;
Cell wall
;
β-Glucan synthesis
;
Kre1p
;
O-glycoprotein
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract The Saccharomyces cerevisiae KRE1 gene encodes a secretory protein required for the production of the cell wall polymer (1 → 6)-β-glucan. Here we report further characterization of the KRE1 gene product, Krelp. A functional, epitope-tagged Krelp is shown to be highly modified in a SEC53-dependent manner. Krelp is O-glycosylated, but the basis for the majority of its post-translational modification is unknown. Fractionation of Kre1p reveals a cell wall-associated form and a less abundant membrane-associated species. Indirect immunoflurorescence demonstrates that Kre1p localizes to the cell surface, where it becomes concentrated at the surface of mother cells. Such a localization of Kre1p seems to parallel the CAL1/CSD2-dependent cell wall deposition of chitin found in S. cerevisiae, and is consistent with evidence from Schizophyllum commune that (1 → 6)-β-glucan accumulates during maturation of the subapical region of the wall distal to the hyphal tip.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00290368
