ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The localization of Drosophila melanogaster ribosomal proteins S14, and 7/8 during oogenesis was studied by indirect immune fluorescence microscopy. The acidic proteins S141 and 7/81 were isolated from D. melanogaster embryonic ribosomal proteins by carboxymethylcellulose chromatography (Chooi 1980). Antibodies raised against each of these proteins were applied to ovariole squashes, and the position of each antibody was localized by fluorescein labeled sheep antirabbit IgGs. Anti-S14 was found predominantly in nurse cell nuclei, follicle cell nuclei, oocytes and, to a much lesser degree, in nurse cell and follicle cell cytoplasm. In contrast, anti-7/8 was found in major quantities in nurse cell and follicle cell cytoplasm, and oocytes. Anti-7/8 in the nurse cell and follicle cell nuclei was either not detectable or at a strikingly lower level than that found in the corresponding cytoplasm. The ‘egg chamber’ patterns of localization of these two proteins were also found in salivary gland cells. However, in Drosophila tissue culture cells, these patterns were altered; both anti-S14 and anti-7/8 were detected only in the cytoplasm.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425457
