ISSN:
1432-2048
Keywords:
DNA polymerase
;
Pisum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Soluble DNA polymerase has been extracted from pea seedlings and partially purified by chromatography on columns of DEAE-cellulose or DEAE-Sephadex. The enzyme elutes from DEAE-cellulose as a single peak, but is fractionated into three peaks, SI, SIa and SII by DEAE-Sephadex chromatography. SIa and SII may be derived from SI by freeze-thaw treatment or by treatment with (NH4)2SO4. The ion and pH requirements and the sensitivity to N-ethyl maleimide of the pea seedling soluble DNA polymerase are similar to those of the DNA polymerase-α from vertebrates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00391168