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Detection of areas of wall differentiation in fungi using fluorescent staining (1974)

Gull, Keith ; Trinci, Anthony P. J.

Springer

Archives of microbiology 96 (1974), S. 53-57

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ISSN: 1432-072X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Fungal colonies were stained with a fluorescent brightner, Calcofluor White M2R New. When viewed using ultraviolet light certain hyphal regions showed intense fluorescence, whereas others showed a lower intensity fluorescence. The apical 10 μm of leadingBotrytis cinerea hyphae showed intense fluorescence. Developing septa and side branches also showed this intense localised fluorescence. Calcofluor staining of hyphae and their subsequent growth illustrated the phenomenon of tip extension.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00590162

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