ISSN:
1432-072X
Keywords:
Trichoderma reesei
;
Cellulose degradation
;
Cellulase
;
β-Glucosidase
;
Continuous flow column
;
Model of cellulose degradation
;
Simulated soil ecosystem
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Trichoderma reesei (Q.M. 9414) was grown in a vertical glass column filled with a mixture of glass beads and cellulose. The column was aerated and maintained at 25° C. A carbon-free nutrient solution, or nutrient solution plus glucose, was passed down the column at a constant flow rate for several 100 h. The effluent solution was collected in a refrigerated fraction collector and assayed for carboxymethylcellulase activity, β-glucosidase activity, extracellular protein and reducing sugar. The pH of the effluent solution and the CO2 content of the effluent gas were also, measured. At the end of one experiment the column was dismantled and the fungus, glass beads and residual cellulose examined by scanning electron microscopy. A mathematical model was formulated by assuming that the column could be approximated to a series of wellmixed flow-through compartments. The model was used to simulate the dynamics of the system and the results generated gave a reasonable qualitative description of some of the experimental results.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00414603
