Publication Date:
2022-05-26
Description:
Author Posting. © The Author(s), 2008. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in Nature Cell Biology 10 (2008): 748-751, doi:10.1038/ncb1738.
Description:
Using laser microsurgery and cell fusion we have explored how additional
centrosomes and/or chromosomes influence the duration of mitosis in human cells. We
find that doubling the chromosome number adds ~10 minutes to a 20 minute division
while doubling the number of centrosomes adds ~30 minutes more, and extra
centrosomes and/or chromosomes prolong mitosis by delaying satisfaction of the
spindle assembly checkpoint. Thus mitosis can be prolonged by non genetic means and
extra chromosomes and centrosomes likely contribute to the elevated mitotic index seen
in many tumors.
Description:
This work was supported by National Institutes of General Medical Sciences grants 40198
(to C.L.R.) and 59363 (to A.K.).
Keywords:
Mitosis
;
Centrosomes
;
Chromosomes
;
Cancer
;
Spindle assembly checkpoint
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
application/pdf
