ISSN:
1432-2099
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Physik
Notizen:
Abstract By the method of gel electrophoresis, radiation-induced DNA single- and double-strand breaks (SSB, DSB) were studied with a model system of pBR322 solution in vitro in the presence of ·OH radical scavengers, mannitol and TE (10–2 mol dm–3 Tris and 10–3 mol dm–3 ethylene diamine tetra-acetic acid). Experiments showed that SSB resulted from one-hit events of radiation energy deposition and DSB resulted from both one-hit and two-hit energy deposition events and so were distinguished into two classes of αDSB and βDSB. Moreover, α/β, where α is the number of DSB per unit dose induced in one irradiation event and β the number of DSB per unit squared dose induced by the combination of two independent SSB, was related to the scavenging capacity, σ, and for σ〉108 s–1,αDSB predominate over DSB. On the other hand, if σ〈2×108 s–1, the measured G(αDSB) decreased in parallel with G(SSB), i.e., G(αDSB)/G(SSB) was a constant. When σ〉2×108 s–1, G(αDSB) decreased slightly so that the ratio of αDSB to SSB evidently increased. Therefore, αDSB could be induced by the radical transfer mechanism for σ〈2×108 s–1 and contrarily produced by the local multiply damaged sites (LMDS) mechanism for σ larger than this value. In addition, the distance for two independent complementary SSB forming a DSB was deduced, but no apparent variation of it was found in the wide σ range from ∼105 to ∼109 s–1, which shows that the DNA steric structure was not influenced by mannitol.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s004110050145
