ISSN:
0741-0581
Keywords:
Embedment media
;
Coagulant and noncoagulant fixatives
;
Intracellular surfaces
;
Scanning electron microscopy
;
Thick sections
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern.
Additional Material:
14 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060020511
