ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
The various methods for chlorophyll determination, found in the literature, may give chlorophyll values differing by as much as 20%. Not only the choice of solvent and equations but also the routines and conditions for processing of samples can be large sources of errors.In the present paper some recommendations are given concerning sampling and storage of plant material, extraction and determination of chlorophylls.A simple and fast method for chlorophyll determinations is described and recommended as a suitable method for most ecological and eco-physiological investigations in which chlorophylls are determined. The principle of the method is that the total amount of chlorophylls is determined in a colorimeter calibrated for chlorophyll measurements. Results from a prototype of the “Chlorophyllometer” are presented and compared with spectrophotometric determinations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1974.tb03743.x