Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Plant, cell & environment
3 (1980), S. 0
ISSN:
1365-3040
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract. Leaf sections (1 × 1 cm) from Chenopodium rubrum L. were floated on Murashige–Skoog medium at constant 20°C and 8800 Lux white fluorescent light. During a period of 4–6 days after inoculation the leaf tissue showed rapid growth and cell division in the mesophyll. Subsequently, after 4 days on a rotary shaker the leaf tissue completely disintegrated and released a great number of single cells into suspension. This procedure, which by-passes the callus culture stage, is well-suited to the rapid production of standardized cell suspension cultures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/1365-3040.ep11580923
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