Publication Date:
1978-09-22
Description:
Binding studies of thyroid hormone to submitochondrial fractions from rat liver suggest that the component responsible for high-affinity, low-capacity (saturable) binding of hormones arises from the inner mitochondrial membrane. The partially purified component, approximately 150,000 daltons, appears to be half protein and half lipid, largely phospholipids, tentatively identified as lecithin, phosphatidyl ethanolamine, and cardiolipin. A similar hormone-binding macromolecule was found in mitochondria from rabbit kidney, from human liver and kidney, and from rat kidney, myocardium, skeletal muscle, intestinal mucosa, whole small intestine, adipose tissue, and lung. It was absent from mitochondria of adult rat brain, spleen, and testis, organs calorigenically unresponsive to thyroid hormones injected in vivo, but was present in mitochondria from brains of rats 12 days old and younger. The organ distribution of the hormone-binding protein and its presence in neonatal brain mitochondria supports the biological relevance of the mitochondrial component as a thyroid hormone receptor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sterling, K -- Lazarus, J H -- Milch, P O -- Sakurada, T -- Brenner, M A -- New York, N.Y. -- Science. 1978 Sep 22;201(4361):1126-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/210507" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Animals, Newborn
;
Brain/metabolism/ultrastructure
;
Cell Fractionation/methods
;
Chromatography, Gel
;
*Digitalis Glycosides
;
*Digitonin
;
Humans
;
Kidney/metabolism/ultrastructure
;
Male
;
Mitochondria/metabolism
;
Mitochondria, Liver/analysis/*metabolism
;
Mitochondria, Muscle/metabolism
;
Rabbits
;
Rats
;
Receptors, Cell Surface/*isolation & purification/metabolism
;
Thyroid Hormones/*metabolism
;
Tissue Distribution
;
Triiodothyronine/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
