ALBERT

Description:    Three cDNA sequences encoding putative opine dehydrogenase (OpDH) enzymes from the mussel Mytilus galloprovincialis were obtained. The deduced amino acid sequences were clearly distinguishable from each other, showing that several OpDH transcripts could occur in the mussel tissues ( p distance 0.46–0.55). When these sequences were aligned and compared with published databank proteins, the range of identity among the M. galloprovincialis OpDH and the strombine dehydrogenase from Ostrea edulis was 51–59 %, the best hit in the three comparisons, followed by OpDH enzymes from other marine invertebrates. Sequence alignment revealed structural motifs possibly related to the binding sites of the substrates. A phylogenetic analysis compared M. galloprovincialis OpDH and annotated sequences belonging to five phyla and seven taxonomic classes, including 19 species, representing the five OpDH protein family members. The phylogenetic tree clustered the OpDH enzymes according to the evolutionary relationships of the species and not to the biochemical reaction catalyzed. Content Type Journal Article Pages 1-12 DOI 10.1007/s10528-012-9551-z Authors Sandra Vázquez-Dorado, Department of Biochemistry, Genetics and Immunology, University of Vigo, Campus Universitario Lagoas-Marcosende, 36310 Vigo, Pontevedra, Spain Alejandro de Carlos, Department of Biochemistry, Genetics and Immunology, University of Vigo, Campus Universitario Lagoas-Marcosende, 36310 Vigo, Pontevedra, Spain Ángel S. Comesaña, Servizo de Determinación Estructural, Proteómica e Xenómica, Unidade de Secuenciación de DNA, Scientific and Technological Research Assistance Centre, University of Vigo, Campus Universitario Lagoas-Marcosende, 36310 Vigo, Pontevedra, Spain Andrés Sanjuán, Department of Biochemistry, Genetics and Immunology, University of Vigo, Campus Universitario Lagoas-Marcosende, 36310 Vigo, Pontevedra, Spain Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928

Description: Influence of Adiponectin and Resistin Gene Polymorphisms on Quantitative Traits Related to Metabolic Syndrome Among Malay, Chinese, and Indian Men in Malaysia Content Type Journal Article Category Note Pages 1-9 DOI 10.1007/s10528-012-9552-y Authors Cia-Hin Lau, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Sekaran Muniandy, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928

Description: Molecular Cloning and Polymorphism of the Major Histocompatibility Complex (MHC) Class IIB Gene of Grass Carp ( Ctenopharyngodon idella ) Content Type Journal Article Category Note Pages 1-8 DOI 10.1007/s10528-012-9549-6 Authors Zhong-Dian Dong, College of Animal Science and Technology, Shandong Agricultural University, Daizong Avenue 61, Taian, 271018 China Yan Zhao, College of Animal Science and Technology, Shandong Agricultural University, Daizong Avenue 61, Taian, 271018 China Qi Fan Zeng, College of Marine Life Science, Ocean University of China, Qingdao, 266003 China Yong Fu, College of Animal Science and Technology, Shandong Agricultural University, Daizong Avenue 61, Taian, 271018 China Fen Na Zhou, College of Animal Science and Technology, Shandong Agricultural University, Daizong Avenue 61, Taian, 271018 China Xiang Shan Ji, College of Animal Science and Technology, Shandong Agricultural University, Daizong Avenue 61, Taian, 271018 China Hui Wang, College of Animal Science and Technology, Shandong Agricultural University, Daizong Avenue 61, Taian, 271018 China Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928

Description:    Eukaryotic translation initiation factors (eIFs) have been shown to be critical in the initiation of protein synthesis. Here, we report the cloning and characterization of a novel gene, LceIF1, from a potentially interesting forage grass, Leymus chinensis (Trin.). The expression results show that LceIF1 is expressed in most organisms under normal conditions, but the transcription patterns differ under sodic-saline and sodic-alkaline stresses. Sodic-saline stress induced a persistent decrease, and sodic-alkaline stress induced overexpression of LceIF1 . Potassic-saline and alkaline stresses did not cause any changes in expression of eIF1. These results indicate that not only pH but also Na + concentration affects overtranscription of LceIF1 . The eIF1 transgenic lines showed relatively high eIF1 expression, resulting in potentially higher stress resistance. Combined with eIF1 transcription in transgenic lines, LceIF1 as a molecular target of salt toxicity is believed to help enhance salt tolerance. Content Type Journal Article Pages 1-18 DOI 10.1007/s10528-012-9546-9 Authors Yan-Lin Sun, School of Life Sciences, Ludong University, Yantai, 264-025 Shandong, China Soon-Kwan Hong, Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon, Kangwon-Do 200-701, Korea Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928

Description:    Murine insulin-like growth factor 2 antisense ( Igf2as ) transcripts originate from the opposite strand of the same Igf2 locus as the Igf2 sense mRNA. The Igf2 , insulin 2 ( Ins2 ), and H19 genes form a cluster of imprinted genes on chromosome 7. Loss of imprinting of IGF2 in humans is associated with Beckwith–Wiedemann syndrome and Silver–Russell syndrome, as well as with Wilm’s tumor and colorectal cancer. We developed a RNA-FISH protocol to detect Igf2as and Igf2 transcripts. The results from the RNA-FISH were confirmed with quantitative real-time PCR and clearly indicate that the Igf2as transcripts are predominantly located in the cytoplasm of C2C12 cells. In a polysome association study, we showed that the Igf2as sedimented with polysomes in a sucrose gradient. The cellular localization of Igf2as transcripts together with polysome fractionation analysis provides compelling evidence that the Igf2as is protein coding. Content Type Journal Article Pages 1-12 DOI 10.1007/s10528-012-9547-8 Authors Carolina Duart-Garcia, Institute of Genetics, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001 Berne, Switzerland Martin H. Braunschweig, Institute of Genetics, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001 Berne, Switzerland Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928

Description:    To determine the prevalence of G6PD deficiency and a Mediterranean mutation among males in southeastern Iran, we studied 1,097 Sistani and Balouch schoolboys. A questionnaire was used to collect demographic data and a history of malaria infection; blood samples were evaluated for G6PD deficiency and the G6PD Mediterranean mutation. Of the 1,097 boys screened, 175 were G6PD deficient (5.8 % of the Sistani boys and 19.3 % of the Balouch boys). The malaria survey indicated that among Balouch subjects, malaria infection was about 14 times that of Sistani subjects. Molecular characterization of G6PD-deficient samples revealed a general frequency of 85.1 % for the Mediterranean variant among all subjects (75 % among Sistani and 86.2 % among Balouch cases). The high prevalence of G6PD deficiency among Balouch populations confirms the hypothesis that the distribution of G6PD deficiency is concordant with the geographic distribution of malaria. Content Type Journal Article Pages 1-8 DOI 10.1007/s10528-012-9548-7 Authors Ebrahim Miri-Moghaddam, Genetics of Non-communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran Yousef Mortazavi, Department of Hematology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran Alireza Nakhaee, Department of Clinical Biochemistry, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran Abdurrashid Khazaei Feizabad, Zahedan University of Medical Sciences, Zahedan, Iran Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928

Description:    Sulfate is important for mammalian development but is not routinely measured in clinical settings. The renal NaS1 sulfate transporter maintains circulating sulfate levels and is linked to renal sulfate wasting in mice. Some autistic individuals exhibit renal sulfate wasting, but the etiology is yet unknown. We measured plasma and urinary sulfate levels, calculated the fractional excretion index (FEI) of sulfate, and screened for two loss-of-function NaS1 sequence variants (R12X and N174S) in 23 autistic individuals. The FEI sulfate values ranged from 0.13 to 0.50. NaS1 variants were detected in 18 of the 23 individuals (11 heterozygous N174S, four homozygous N174S, two heterozygous R12X, and one individual carried both R12X and N174S). Those individuals with neither sequence variant had FEI sulfate ≤ 0.34, whereas FEI sulfate ≥ 0.35 was found in about 60 % (11 of 18) of individuals that had R12X and/or N174S. This study links renal sulfate wasting with loss-of-function NaS1 sequence variants in humans. Content Type Journal Article Pages 1-7 DOI 10.1007/s10528-012-9550-0 Authors Francis G. Bowling, Mater Children’s Hospital, Mater Health Services, South Brisbane, QLD 4102, Australia Helen S. Heussler, Mater Children’s Hospital, Mater Health Services, South Brisbane, QLD 4102, Australia Avis McWhinney, Pathology Department, Mater Health Services, South Brisbane, QLD 4102, Australia Paul A. Dawson, Mater Medical Research Institute, South Brisbane, QLD, Australia Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928

Description: Molecular Studies on the Origin of the Hb G-Coushatta Mutation in Denizli Province of Turkey Content Type Journal Article Category Note Pages 1-5 DOI 10.1007/s10528-012-9543-z Authors Aylin Köseler, Department of Biophysics, Medical Faculty, Pamukkale University, Kınıklı, 20020 Denizli, Turkey Onur Öztürk, Department of Biophysics, Selçuklu Medical Faculty, Selçuklu Üniversitesi, Konya, Turkey Ayfer Atalay, Department of Biophysics, Medical Faculty, Pamukkale University, Kınıklı, 20020 Denizli, Turkey Erol Ö. Atalay, Department of Biophysics, Medical Faculty, Pamukkale University, Kınıklı, 20020 Denizli, Turkey Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928

Description:    PON1 and PON2 have attracted considerable attention as candidate genes for coronary heart disease because their enzymes function as key factors in lipoprotein catabolism pathways. We studied the distribution of PON1 and PON2 polymorphisms, including genotyping, lipid profile, and PON1 activity, and their association with PON1 activity and significant coronary stenosis (SCS) in a Tunisian population. PON1 activity was lower in patients with SCS than in controls. It increased with the R allele (QQ 〈 QR 〈 RR) in PON1 -192 genotypes and with the L allele (MM 〈 ML 〈 LL) in PON1 -55 genotypes. In the presence of metabolic syndrome and diabetes, PON1 -192RR and PON2 -311CC were associated with an increased risk of SCS and PON1 -55MM seems to have lower risk. This association was evident among nonsmokers for PON1 -55MM and among smokers for PON1 -192RR and PON2 -311CC. The GTGC haplotype seemed to increase the risk of SCS compared with the wild haplotype in a Tunisian population. Content Type Journal Article Pages 1-16 DOI 10.1007/s10528-012-9544-y Authors Jihène Rejeb, Department of Biochemistry, UR MSP 28/04, Sahloul University Hospital, 4054 Sousse, Tunisia Asma Omezzine, Department of Biochemistry, UR MSP 28/04, Sahloul University Hospital, 4054 Sousse, Tunisia Lamia Rebhi, Department of Biochemistry, UR MSP 28/04, Sahloul University Hospital, 4054 Sousse, Tunisia Imen Boumaiza, Department of Biochemistry, UR MSP 28/04, Sahloul University Hospital, 4054 Sousse, Tunisia Hajer Mabrouk, Laboratory of Biochemistry-Toxicology, University Hospital Fattouma Bourguiba, Monastir, Tunisia Hamida Rhif, Department of Biochemistry, UR MSP 28/04, Sahloul University Hospital, 4054 Sousse, Tunisia Nabila Ben Rejeb, Department of Biochemistry, UR MSP 28/04, Sahloul University Hospital, 4054 Sousse, Tunisia Naoufel Nabli, Department of Biochemistry, UR MSP 28/04, Sahloul University Hospital, 4054 Sousse, Tunisia Wahiba Douki, Laboratory of Biochemistry-Toxicology, University Hospital Fattouma Bourguiba, Monastir, Tunisia Ahmed Ben Abdelaziz, Information System Direction, Sahloul University Hospital, Sousse, Tunisia Essia Boughzala, Department of Cardiology, Sahloul University Hospital, Sousse, Tunisia Ali Bouslama, Department of Biochemistry, UR MSP 28/04, Sahloul University Hospital, 4054 Sousse, Tunisia Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928

Description: A Simple and Affordable Method of DNA Extraction from Fish Scales for Polymerase Chain Reaction Content Type Journal Article Category Note Pages 1-6 DOI 10.1007/s10528-012-9539-8 Authors Yanhe Li, College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 China Yasmeen Gul, College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 China Zexia Gao, College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 China Wei Luo, College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 China Weimin Wang, College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070 China Journal Biochemical Genetics Online ISSN 1573-4927 Print ISSN 0006-2928