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  • Cell & Developmental Biology
  • EARTH RESOURCES AND REMOTE SENSING
  • 2010-2014
  • 1925-1929  (172)
  • 1900-1904  (28)
  • 101
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 46 (1928), S. 217-239 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Notwithstanding the fact that several species of Bruchidae have been used by geneticists for several years, no cytological studies have as yet been made on any member of this family of the Coleoptera. The present paper gives a general account of the spermatogenesis of Bruchus quadrimaculatus Fabr.The spermatogonia undergo two mitotic divisions. After the second division, the nuclei remain small and very dense for some time before the beginning of the growth phase. During this interval the nuclei do not assume again the characteristics of the interkinesis stages. In the primary spermatocytes typical tetrads are formed. The chromosomes are asymmetrically V-shaped. The end of one arm of the ‘V’ fuses with the end of the corresponding arm of its synaptic mate. Disjunction takes place in the primary spermatocyte division. After the division of the secondary spermatocytes, the chromosomes become vesicular and form a reticular nucleus in the spermatid, after which the chromatin becomes deposited as a chromatin rim around the nuclear periphery. The diploid number of chromosomes is nineteen in spermatogonia and in male somatic cells, and twenty in female somatic cells. An unpaired X chromosome is present in the spermatogonia, which fails to divide in the primary spermatocyte division, but passes as a whole to one pole in advance of the autosomes. The X chromosome divides normally in the secondary spermatocyte division with the autosomes.In the method of sex determination, Bruchus does not follow the method of the majority of beetles, since most of those studied adhere to the X-Y type.
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  • 102
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 47 (1929) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 103
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 47 (1929), S. 1-36 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The first spermatocytes of Circotettix verruculatus have eleven chromosomes. Five of these are regularly atelomitic and three are telomitic. The other three are variable and may have both diads telomitic, both atelomitic, or one diad telomitic while the other is atelomitic. The locus of fiber attachment and, consequently, the form of the chromosome are constant for the individual. The point of fiber insertion is known to be inherited according to mendelian principles, and is, therefore, a measure of the frequencies of the telomitic and atelomitic diads in a group of individuals.Samples of five geographically different populations were studied with respect to the proportion of atelomitic to telomitic diads in the three variable tetrads. The proportion for each of the three chromosomes was compared with that for the corresponding tetrads from the other localities. The data were subjected to statistical analysis, and significant differences between some of the groups were found in the proportion of atelomitic to telomitic diads in corresponding chromosomes.A possible correlation between these cytological differences among the various localities and the formation of geographical races or subspecies are discussed. An inversion of the portion of a chromosome, which causes an apparent, although not a real, change in the locus of fiber attachment, is suggested as the origin of atelomitic chromosomes. The effect which heteromorphism of synaptic mates may have on crossing-over is considered.
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  • 104
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 48 (1929), S. 385-431 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Direction of peristalsis of the dorsal vessel in prepupa, chrysalis, and adult of Lepidoptera is not constantly forward; phases of forward peristalsis alternate with phases either of backward peristalsis throughout the whole dorsal vessel or of diverging waves originating in the third to fourth abdominal segments, proceeding backward and forward. Periodic reversal in the silkworm begins twenty-four hours after spinning, forty-eight before pupation. Pupation occurs during a vigorous backward phase. Soon after pupation, and sometimes during pupation, backward phases consist of diverging waves. Later, and in the adult, complete reversal intermittently occurs. Backward phases in the pupa are long (e.g., fifteen minutes), the average rate slow and variable, quickening at eight to ten days to that of the adult. Any long backward phase shows a gradual slowing down, except approaching pupation when the pulse quickens to equal that of forward beating. Average rate of forward beating is constant at constant temperature, but absolute rate in a long phase gradually slackens. Pauses (sometimes one and one-half hours long in older pupae) often follow forward phases. Alternating phases in the adult, compared with the pupa, are short, about one minute and usually less than one hundred beats. In old moths the phases become still shorter, often being reduced to single beats. Opposing waves occasionally conflict. Periodic reversal occurs for hours after excision of the dorsal wall with the pericardium and heart. As in ascidians, back pressure cannot explain reversal.
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  • 105
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 16 (1900), S. 369-458 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 106
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    Journal of Morphology 16 (1900), S. 269-322 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 107
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    Journal of Morphology 16 (1900) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 108
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    Journal of Morphology 17 (1901), S. 293-350 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 109
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    Journal of Morphology 17 (1901), S. 351-380 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 110
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    Journal of Morphology 18 (1903) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 111
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 112
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    Journal of Morphology 40 (1925) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 113
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    Journal of Morphology 40 (1925), S. 517-557 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An early segregation of germ cells and migration through a germ track into the gonad does not occur in the albino rat. The germ cells are produced only from the peritoneum of the genital region and their earliest formation is coincident with the thickening of the coelomic epithelium to form the genital ridge. This takes place eleven days after insemination in embryos of approximately 18 somites. Germ cells continue to form from the peritoneum during the early development of the gonad. The peritoneum of this region also produces mesenchyme, smaller cells of the gonads, and the germinal epithelium.The argument for the specific character of the germ cells in vertebrates and their continuity from the egg is based largely upon assumption, and not upon substantial observations, and must be discarded. Germ-cell origin is a problem of cellular differentiatio, and not of early segregation.
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  • 114
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    Journal of Morphology 41 (1925), S. ii 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 115
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    Journal of Morphology 41 (1925), S. 191-216 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Painted turtles, gopher tortoises, and terrapins were fed on various mixtures of sand, salts, dextrin, casein, cod-liver oil, wheat, eggs, lettuce, and meal worms. Each individual was weighted weekly for about a year and then killed for analysis, the water, ash, nitrogen, and fat being determined. Some individuals increased in weight as much as 75 per cent, others lost weight. Judged by growth and chemical analyses, the food requirements of chelonians, as representative poikilothermal vertebrates toward nutritive substances (including vitamines) are similar to those of homoiothermal animals.
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  • 116
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    Journal of Morphology 41 (1925), S. 267-281 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Of the eggs laid by Fasciolaria about 1 per cent develop into veligers, about 2 per cent more undergo a few cleavage divisions, and about 97 per cent do not divide at all. The 99 per cent that fail to develop normally are ‘swallowed’ by the veligers. This study concerns itself chiefly with the ova that do not divide. Notes on normal development and on the ova that undergo atypical cleavage are included.All of the ova are found to be typical when passed from the ovary. To each ovum one to several sperms become attached at the vegetal pole in the region of a mass of undifferentiated protoplasm - the ‘polar mass.’ A fertilization cone forms in each ovum and a fertilization membrane. In typical development a yolk lobe is formed, the sperm enters in the usual way, and fertilization is completed as in many other mollusks. In 97 per cent of the ova the yolk lobe is not formed and the sperm does not enter. In these cases the wall of the egg nucleus remains intact a long time. The nucleus itself and the ‘polar mass’ sink into the egg and meet at the center. Then the nuclear wall disappears and an atypical diaster is formed. However, cleavage is not begun and the chromosomes form vesicles that remain near the center of the ovum until it is ‘swallowed’ and digested by the veliger.
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  • 117
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In general, the changes of locomotor rate of Amoeba under the influence of temperature are similar to the effects of temperature in other biological processes.The rate increases with rising and decreases with falling temperatures within a certain temperature range, this being, for the forms investigated, the interval between 6° and 23.5°C. In some individuals, however, these limits were found to be wider, extending from 2° to 26°. There is an average optimum at about 23.5°, beyond which an increase in temperature is followed by a decrease in rate. The immediate response of Amoeba to a change of temperature is variable, due, as was shown in a previous paper, to the fact that locomotor-rate changes are rhythmic. The effect of a change of temperature is thus conditioned not only by the enviornmental factors, but also by the phases of the locomotor rhythm. This fact also makes it difficult to establish the exact quantitative relationship which exists between a change of temperature and the locomotor rate - an aspect which will be developed in a future paper.
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  • 118
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    Journal of Morphology 42 (1926), S. 253-305 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The testis is differentiated from the indifferent gonad in embryos of fourteen days. The indifferent gonad and the sex cords contain large and small cells, both of which are derived from the coelomic epithelium. The large cells have generally been called primordial germ cells, they increase by mitosis and reach their maximum in embryos of seventeen days. But all the large cells degenerate and have completely disappeared by nine days after birth. The large cells, therefore, do not contribute to the formation of cells in the tubules of the adult testis. After the disappearance of the large cells, the solid seminiferous tubules are composed of indifferent epithelial cells of a uniform type, which are descendants of the small cells of the genital ridge, and which later form both the germ cells and the Sertoli cells of the seminiferous tubules of the adult testis.Definitive spermatogonia begin to form fifteen days after birth, when the solid cords are becoming hollow tubules; spermatocytes occur at twenty-three days, when the testes are entering the inguinal canals; spermatids are first observed about thirty-three days, when the testes enter the scrotum, and perfect spermatozoa at forty-eight days. Sertoli cells cannot be clearly distinguished until almost puberty; these cells may be only indifferent epithelial cells. During the development of the seminiferous tubules, a good many cells of all stages of spermatogenesis degenerate.
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  • 119
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    Journal of Morphology 42 (1926) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 120
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The claspers of Centrina are adnate with the pelvic fin and bear a spine as in other Spinacidae. Mustelus canis resembles M. lunulatus rather than M. vulgaris. The claspers of Chiloscyllium end in a pointed spike. Pseudotriakis resembles the Carchariidae. The three North American Atlantic species of the genus Raia are considered, and R. laevis and R. erinacea are placed in the pseudogenus containing R. batis, and a new pseudogenus erected for R. ocellata. A gross and histological account is given of the Cowper's glands of Homo, and they are shown to be homoplastic with clasper glands, similar in structure, arrangement, development, and function.
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  • 121
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    Journal of Morphology 44 (1927), S. 363-372 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The history of investigations on the contractile vacuole is reviewed briefly and brought up to date.The study of the contractile vacuole in Amoeba proteus is considered from standpoints of origin, structure, behavior, and function. The results are obtained from a prolonged study of normal organisms and from their reactions when introduced into conductivity water.The origin of vacuoles is studied by means of dark-field illumination which reveals the vacuole to be formed from a fusion and coalescence of extremely minute droplets.The retaining ‘wall’ of the contractile vacuole is not a permanent structure, but is in the nature of a condensation membrane, totally disappearing with each contraction.The loci of the contractile vacuoles are not permanent, but vacuoles are formed more or less at random. It is unlikely that they are supported in gelated areas, for amoebae with a dozen vacuoles are quite active and there is no interference with amoeboid movement.Conductivity water increases the size, number, and rate of contraction of contractile vacuoles, which suggests that they may function in maintaining an osmotic gradient as well as in the elimination of metabolic waste.
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  • 122
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    Journal of Morphology 43 (1927), S. 267-297 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: All phases of mitosis have been observed in the ciliated cells of the pharynx and esophagus of the tadpole of the green frog, Rana clamitans. Centrosomes have been observed in ciliated cells. These data contravene the hypothesis of Lenhossék and Henneguy to the effect that ciliated cells cannot divide by mitosis because they have lost their centrosomes in the formation of the basal corpuscles to which the cilia are connected, and the corollary to this hypothesis (Jordan, '13), that in consequence ciliated cells must divide by amitosis. From counts of cells with cilia, those without cilia but with basal corpuscles, and those without either cilia or basal corpuscles in different phases of mitosis, it is inferred that the cilia are lost during mitosis.Cilia sprout from basal corpuscles, and not from mitochondria in cells undergoing ciliogenesis. From the presence of diplosomes in the distal region of cells in which basal corpuscles are developing, it is assumed that the basal corpuscles arise by partition of the centrosome of the preciliated cell. The centrosome, however, does not become completely lost by reason of this process, but retains enough vitality to insure mitosis. Amitosis, which occurs in some of the ciliated cells, results, according to these data, from degree of differentiation, not from structural deficiency.
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  • 123
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    Journal of Morphology 43 (1927), S. 427-497 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The primordial germ cells are derived from entodermal giant cells which are found first before the gut is formed and later along the ventral and lateral margins of the gut. Some of these cells pass through the lateral mesoderm to a position dorsal to the gut, where they are distinctly recognizable as germ cells. They are then shifted to the gonad region.Sex could be distinguished first at fifty-two days. The female sex is indicated by early maturation stages and the beginning of the oviducal groove. The male sex is distinguished by the presence of the sperm duct. No tendency toward juvenile hermaphroditism is apparent.A portion of the oocytes formed during the first season matures for the first spawning, which takes place at the age of two years. The remainder form a reserve supply, which is increased each year by oocytes formed from dormant oogonia.Maturation in the male begins in September and continues until spawning-time in April. Spermatogonia lying dormant within the cysts during maturation give rise to the sperm of the next season.‘Spermatid masses,’ probably formed by the fusion of spermatids, are found in the cysts with the ripening sperm.Definitive sex cells in both sexes have their origin only in primordial germ cells. No transition from somatic cells to germ cells was found at any stage.
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  • 124
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    Journal of Morphology 40 (1925), S. 1-109 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Much more information than is available at present must be obtained before the phylogeny of the muscular system in the Teleostei can be worked out satisfactorily. As a step toward the solution of this problem, the present study gives a detailed description of twenty cranial muscles in a number of species belonging to three groups of cypriniform fishes: the Cyprinoids, Cobitoids, and Siluroids.Particular attention is given to the morphological relations of the following muscles: adductor mandibulae, geniohyoideus, levator arcus branchialis, adductor arcus palatini, retractor branchialis dorsalis, interarcualis dorsalis and the trapezius.Comparison is made between corresponding muscles in different members of the three groups of fishes, and various homologies are pointed out between muscles in cypriniform and those in other fishes, especially Amia, Scomber, Perca and Esox.
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  • 125
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    Journal of Morphology 40 (1925), S. 235-259 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A study of the peripheral nerves of the earthworm shows that one pair of nerve trunks arise from the lateral regions of the cerebral ganglion, one pair from near the lateral, and two pairs from the ventral region of the circumpharyngeal connectives. These supply, in the order given, the prostomium, segment 1 and segment 2. Segment 3 and each succeeding segment, except the last, are supplied with three pairs of nerve trunks which arise from the nerve cord in the segment concerned. None of the nerve trunks of the body was observed to send branches to more than one segment, which shows that they are segmental in origin and distribution.The anterior portion of the central nervous system has undergone caudal migration and modification of its ganglia. Some of the nerve trunks have disappeared through atrophy, while others have become fused at their bases.A subepidermal nerve plexus is found at the base of the epidermal cells from which nerve fibers pass to the internal nerve net and to the central nervous system. This plexus also sends intercellular and intracellular fibers into the epidermis.The so-called sense cells of the epidermis appear to be the cell bodies of sensory neurones which send nerve fibers to the ganglia of the central nervous system, where they form synaptic connections with other neurones.There is an enteric nerve plexus in the wall of the alimentary canal which is directly connected to the circumpharyngeal connectives.
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  • 126
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In conjugation fusion occurs along the entire oral surfaces of the proboscides of Dileptus gigas. Two size-reducing divisions occur in rapid succession immediately preceding conjugation. Only one of the many micronuclei takes part in the process of nuclear reorganization. All other chromatic material is massed at this time in the posterior portions of the conjugants. The pronuclei are derived from the single active micronucleus, and interchange occurs immediately preceding the separation of the mating individuals. The fertilization nucleus divides to form two nuclei of diverse size. The smaller one produces thirty-two or sixty-four micronuclei, while the larger one divides to produce a like number of macronuclei, each of which finally breaks up into many chromatic granules which form the numerous densely staining nuclear derivatives which are characteristic of the vegetative stage of Dileptus gigas.In the early stages of this reorganization process specimens are frequently found with from two to eight distinct nuclei often arranged in a series as in a beaded nucleus. This condition probably explains the frequent references in literature regarding such a nuclear condition in Dileptus.Dileptus gigas has, accordingly, in the vegetative stage, a multinucleate condition with reference to the micronucleus and a fragmented or distributed condition with reference to the macronucleus.
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  • 127
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    Journal of Morphology 45 (1928) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 128
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Amoeba proteus was raised in a modified and diluted Ringer solution. When the pH of this culture medium became less than 6.0, the normal activities of the amoebae were interfered with; and when a still lower pH was attained, the amoebae died off. The same was true when the pH became greater than 8.0. At neutrality the activities were subnormal, very dark, and rounded. The rate of locomotion of amoebae raised in solutions with a pH less than 7.0 showed a maximum rate of locomotion at pH 6.6, which decreased as the pH changed in either direction, dropping to a very low rate at pH 7.0 and above and also below 6.0. For amoebae raised at a pH above 7.0 the rate was maximum at pH 7.6 and decreased as the pH changed in either direction; it was low at pH 7.0 and below and also above 8.0.On increasing the external osmotic pressure of the medium it was found that the effects caused varied somewhat with the hydrogen-ion concentration. Small increases in osmotic pressure decreased the rate from the normal at pH 6.0 and 8.0, increased it at pH 6.6 and 7.6, and did not affect it at pH 7.0. Osmotic pressures above that produced by M/20 lactose caused locomotion to cease in a short time at all pH values.
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  • 129
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    Journal of Morphology 45 (1928), S. 209-231 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The highly specialized cranial musculature of the toadfish is characterized by the following features: 1Absence of intermandibularis and branchiomandibularis muscles.2Presence of levator premaxillaris muscle.3Very large branchial chamber, the outer wall of which is formed by seven branchiostegal rays connected by a strong fascia provided with muscles (oblique levators and adductors).4Highly developed masticator muscles (adductor mandibularis and pterygoids).5The rectus abdominis, sternohyoid, and hyohyoid muscles are attached by a median aponeurosis to the hyoid and basibranchial elements and directly to the hypobranchial cartilages; this muscle complex depresses the buccal floor in opposition to the geniohyoid.6The pelvic fins are in the jugular position.7Two narrow muscles connect the cleithrum with the fourth ceratobranchial.8The cranial musculature is obviously adapted to a carnivorous habit and particularly for increasing respiratory capacity under asphyxial conditions.
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  • 130
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    Journal of Morphology 45 (1928), S. 293-398 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Blindfolded persons walk, run, swim, row, and drive automobiles in clock-spring spiral paths of greater or less regularity when attempting a straightaway. The spirals turn either right or left in one and the same individual, and may do so even in one experiment. But either right or left turns predominate in the great majority of individuals, often to a high degree. The paths show marked individuality, and there is some ground for thinking there exists a correlation between temperamental differences and general character of path.The mechanism which produces the spiral path is not located in the locomotor organs, but in the central nervous system and is probably identical essentially with the spiral mechanism in other motile organisms, all of which move in spiral paths when there are no guiding senses to direct the path. The clock-spring spiral in man is interpreted as the expression in two dimensions of space of a helical spiral mechanism which seems to exist in all motile organisms moving in three dimensions of space and in amebas which move in two dimensions. In a large number of lower organisms the number of body lengths per spiral turn is almost constant, being about 4.5. The smallest regular swimming spirals in man are very close to this value, but the smallest regular walking spirals are somewhat larger. The fundamental spiral mechanism seems to be of molecular dimensions, and there seems to exist a demonstrable locomotor bilateral asymmetry in very nearly, if not quite, all organisms.
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    Journal of Morphology 45 (1928), S. 473-503 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A detailed study has been made of the anatomy of one of the fingernail shells, and preliminary observations on the life-history have been carried out. In its general organization Sphaerium notatum is very similar to the larger fresh-water lamellibranchs. A gastric shield, crystalline style, and style sac, very similar to those found in the stomach and intestine of Lampsilis, are present. A pair of slender muscles extending from the dorsal side of the body into the gills, and evidently not previously described, have been found. The nervous system consists of the typical three pairs of lamellibranchiate ganglia, with their connectives, accessory ganglia, and nerve fibers. Particular study was given to the statocysts and osphradia, and attention is called to the fact that the function commonly ascribed to the osphradia is incompatible with their position in the roof of the cloacal chamber.S. notatum, like all the Sphaeriidae, is hermaphroditic and viviparous. The gonads are paired racemose glands lying behind and below the stomach. The sperm-producing follicles form the anterior portion of each gonad and are somewhat smaller and more numerous than the ova-producing follicles which form the posterior portion. The young pass through the early stages of development in brood pouches in the gills and are expelled as relatively enormous individuals.Preliminary observations on the life-history indicate that reproduction reaches its height in the summer and that fertilization probably takes place during the late summer and fall.
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    Journal of Morphology 45 (1928), S. 579-597 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: When Menidia eggs are fertilized with Prionotus sperm, the Prionotus chromosomes react in the Menidia cytoplasmic medium just as they do in the cytoplasm of Fundulus eggs. There is lagging, non-disjunction, and elimination of chromosomes during the early cell divisions. The mitotic behavior of the Prionotus sperm in the Menidia egg also resembles the behavior of the sperm of Ctenolabrus in the same medium. This behavior which was expected from what was known concerning the mitotic behavior of the reciprocal crosses between Menidia and Fundulus and between Ctenolabrus and Prionotus and other intercrosses between the members of these two groups is regarded as a function of the physical state of the egg cytoplasm during the division phase of mitosis. This physical character forms the earliest differential factor in the development of these hybrids and shows no correlation with the width of the cross.A comparison of nine teleost crosses, in which both the development and the early mitotic behavior are known, with a rough numerical estimate of the width of the cross brought out the fact that development is most successful in crosses between nearly related species if mitosis is normal and in distantly related crosses if mitosis is abnormal. This indicates that nuclear relationship is also a factor in the development of hybrids.
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    Notes: Virulent hay-infusion cultures of Bacillus pyocyaneus are toxic to pure-line races of three species of paramecia, but these races may acquire a tolerance for this toxic agent. Races with acquired tolerance have been grown for long periods of time in toxic, pure cultures of B. pyocyaneus by means of the daily-isolation culture method, and here the average division rate is as high as, or higher than, in the chance-mixed bacterial cultures in which these protozoa are usually maintained in the laboratory. The tolerance is lost, however, when the paramecia are removed from the toxic cultures and grown for a number of generations in cultures of non-toxic bacteria.The toxic agent that is lethal to paramecía is probably the soluble toxin of B. pyocyaneus. The investigation shows that the agent is soluble and either thermolabile or volatile. It also shows that all deleterious substances, other than the soluble toxin, known to be produced in cultures of this bacillus, are non-lethal to paramecia.Hay-infusion cultures of Bacillus enteritidis were lethal to paramecia. All attempts to develop tolerance in paramecia for the toxic agent in these cultures failed.Under the experimental conditions that prevailed, diphtheria toxin was found to have no appreciable effect upon the division rate or death rate in three species of paramecia.
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    Notes: The following species of earwigs are used in this study: Labidura bidens, Labia minor, Anisolabis annulipes, Anisolabis maritima, and Forficula auricularia.1In all species the chromosomes are divisible into, a) autosomes and, b) XY-complex.2The chromosome distribution in regular in Labidura bidens and Labia minor. The male diploid number is 12 and 14, respectively. Each has an XY-complex in which the X is a single chromosome.3In both Anisolabis annulipes and Anisolabis maritima the male diploid number is 25, or 22 autosomes and an XXY-complex. The two X components remain fused during the first spermatocyte division.4The diploid number in the male of Forficula auricularia is 25 and 24. The chromosome number is constant in the individual. The irregularity is interpreted as due to the fusion of the two X components in the individuals with 24 counts and to these X components remaining separate in the earwigs with the 25 counts.5An explanation is given for some of the variable results obtained in former studies of the chromosomes of Forficula auricularia.6The discussion considers the possible origin of the variations in chromosome numbers in the earwigs.
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    The breeding season of the opossum (Didelphis virginiana) and the rate of intrauterine and postnatal developmentThe work on the opossum was begun at the suggestion of Dr. J. T. Patterson, Professor of Zoölogy, the University of Texas, about 1913. It was prosecuted intensively through the generous financial aid and moral support of The Wistar Institute, Dr. M. J. Greenman, Director, and with the assistance of Dr. C. H. Heuser, then fellow of The Wistar Institute. It is to the skill of Doctor Heuser that most of the photographs presented in the four plates accompanying this article and former papers of this series are due. Some of the photographs were taken from fresh living material in January and February of 1917 at Austin, Texas. The embryological investigations soon gave way in large measure to physiological studies in which the following generously aided: Mr. H. A. Wrocbanker, and Mr. Herman Becker, merchant, Austin, Texas; the University of Texas, Department of Zoölogy; The Bache Fund of the National Academy of Science. I take this opportunity of reiterating my indebtedness to these sources of the necessary nervus rerun to carry on the work and for the spirit of helpfulness in which the grants were made. The Wistar Institute is the repository of most of the material collected and will supervise its study in the future. The present writer can primsie only two more installmetns of these ‘studies’: one on the origin of the mesoderm and the chorda dorsalis, the other on pathological ova of the primitive-streak stage and earlier. (1928)
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    Journal of Morphology 46 (1928), S. 143-215 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Notes: The breeding season of the opossum at Austin, Texas, begins in January, following a three months' anoestrous period. The modal point for ovulation days is reached in the third week. The rate of intra-uterine development was investigated chiefly by surgical removal of one uterus, noting the stage attained by the ova therein and allowing the surviving uterus to incubate its ova a precalculated period of time. Unique charts epitomize the results. The primitive-streak stage is completed, the medullary groove and chorda begin at seven and one-half days post coitum, seven days post ovulationem, leaving only five and one-half days' actual development of the embryo to birth. The rate of development is compared with Eutherian mammals.The curve of postnatal growth has the shape of embryonic growth curve of higher mammals. The eyes and lips open, at about fifty days (young the size of mice). At this time the young leave the teat for the first time, but are not weaned for about thirty days more. Soon after weaning, the mother may become pregnant again. At ninety to one hundred days (young size of large rats), the young may begin to shift for themselves.
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    Journal of Morphology 42 (1926), S. 453-471 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The activities of the larva and the responses and orientations it makes to external stimulation, and the structural organization by which these activities are produced, are described and figured.Existing systematic confusion of M. citrina with other species (M. manhattensis, M. nana, and M. microsiphonica) is cleared. The duration of the period of larval life is found to vary between 5 and 170 minutes. A proportionally small number retain the larval form a longer time. During larval life, periods of swimming movements alternate with periods of inactivity, the latter, at first of momentary duration, becoming longer and longer until activity ceases.No light receptor exists and no response to light is made. A statolith is found in the sensory vesicle, and frequent geonegative orientations are made during the free-swimming period. These are of short duration and tend to occur, 1) when it emerges from the parent; 2) at the beginning of each of the frequently recurring periods of swimming activity; 3) immediately upon making contacts. Unoriented movements follow each orientation and constitute much the greater part of behavior.The larva lacks definite organs of attachment. The entire surface of the tunic becomes adhesive at the time of metamorphosis. Only those structures that make up the action systems of the larva are fully differentiated, all other parts are embryonic in condition.
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    Notes: The integument of the vermilion-spotted newt contains three kinds of pigment--yellow, red, and black. The yellow is uniform and continuous over the whole body; the red and black are discrete, and are chiefly in large spots. The pattern does not change measurably in an adult individual.Functional changes in contraction of the scattered dorsal melanophores were uncommon. The only efficient single factors found were a long subjection to low temperature (expansion) and injection of pituitrin (contraction).Removal of skin was followed by a rapid mass migration of dermal elements into the wound area. Melanophores were thus furnished to a dorsal wound, and after several months black spots formed from these. Red pigment never regenerated nor migrated into a wound. Yellow pigment was formed in situ after the wound had completely healed.Auto-, homo-, and heterotransplants lost their pigment patterns and were gradually reorganized so as to conform in gross appearance to the surrounding pattern of the individual host's skin. The transformation was never complete in eight months, but new black spots and new yellow pigment eventually formed.The behavior of melanophores during morphogenesis differed for specific areas of the integument. Their movements and aggregation were highly coordinated among themselves.
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    Notes: In the epidermal cells of green-frog tadpoles (Rana clamitans) there are present coarse, conspicuous mitochondrial threads. In the dorsal body regions, epidermal cells of the middle layers also contain pigment granules, grouped in crescentic masses in the distal portions of the cells.Administration of thyroid extract results in permanent disappearance of the mitochondrial threads and disappearance to a large extent of the epidermal pigment granules. Processes of cellular dedifferentiation and proliferation occur rapidly over widespread areas, the mitochondria undergoing intracellular resorption. A new type of epidermis is developed containing many cutaneous glands, adapted for the approaching terrestrial life.Wound infliction induces similar processes of cellular dedifferentiation and proliferation in the epidermis in the immediate vicinity of the wound. Cells in this region lose their mitochondrial threads by intracellular resorption, and there is also some disappearance of pigment granules. The new epidermal cells in the early stages of regeneration produce neither mitochondrial threads nor pigment. This condition is not permanent, however, and in the later stages the larval characteristics appear again, both mitochondrial threads and pigment being redifferentiated.In the hyperthyroid animals there is also a significant mobilization of mesenchymal chromatophores, correlated to some extent with the loss of epidermal pigment.The significance of epidermal changes is discussed with reference to cutaneous abnormalities associated with hyperthyroid and hypothyroid conditions.
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    Journal of Morphology 43 (1926), S. 147-179 
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    Notes: Animal posture cannot be regarded as an actual inheritance, for it constitutes a current physiological interaction between gravity and an organism, according to the structure and physical powers of the latter. Hence, ‘inherited posture’ merely implies that in a given species physical qualifications are congenitally transmitted, which facilitate a certain characteristic position of the body. Evolution of posture, therefore, has inevitably been associated with corresponding evolutionary changes in organic structures.Evidence furnished by the application of biomechanics to studies of ancient and modern primate structures indicates that man's erectly supported body posture could only have originated from a vertically suspended posture (arboreal).The fact that the prehuman stem passed through an earlier arboreal and brachiating period is attested to by the grasping character of his hands, the ratio of arm-body length, the extreme mobility of the shoulder-joints, as well as the extension of his legs on the body.The semierect posture of the great apes is not an advance toward human bipedism, but a modern reversion toward quadrupedism.Postural evidence conforms with the many other lines of testimony which maintain the close relationship of the human and anthropoid stems, and signifies that man has been an erect terrestrial biped since the time of his physical origin.
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    Journal of Morphology 43 (1927), S. 347-385 
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    Notes: The development of the thigh musculature in a series of chick embryos is described and figured. In the earliest the muscular tissue is in the form of two distinct masses lying on opposite surfaces of the limb. Later, both divide into proximal and distal portions at the knee. The proximal portions, by a series of divisions, gradually attain the condition found in the adult thigh.The embryological findings tend to support the theory of the derivation of tetrapod limb musculature from the two opposed (dorsal and ventral) muscle masses of the paired fins of bony fish.The reptilian homologies of the ilio-trochanterici cannot be definitely ascertained from embryological evidence.The ischio-femoralis (= ischio-trochantericus), Previously regarded as dorsal, and the coccygeo-femorales, previously classed as incertae sedis, are in reality members of the ventral group.The distinction between ‘intrinsic’ and ‘extrinsic’ muscles inserting on the free limb appears to have no embryological or phylogenetic basis in fact.Double innervation (motor) is a primitive condition in tetrapods.Rotation of the avian pubis is correlated with an improved functioning of the obturator in the rotated position coupled with a lack of interference with the other musculature concerned.
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    Journal of Morphology 43 (1927), S. 547-555 
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    Notes: The diploid number of chromosomes obtained from counts of anaphases of the first somatic mitosis is found to be forty-four. Of these, seven have terminal, thirty-seven non-terminal attachment, giving a distribution of seven rods, thirteen V's, and twenty-four J's. The number is constant in all the fertilized eggs counted, indicating an XX-XY arrangement of the sex chromosomes.
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    Journal of Morphology 44 (1927), S. 21-28 
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    Notes: Experiments designed to ascertain the effect of hydrogen-ion concentration on encystment in Didinium were carried out by depriving didinia of food in mixtures of spring water and buffer solutions whose hydrogen-ion concentrations varied from pH 5.0 to pH 9.6, and by counting the number of didinia which encysted and the number which remained active and ultimately died of starvation.The maximum percentage of encystment was attained between pH 6.4 and 8.4, the range in hydrogen-ion concentration which is also most favorable for the growth of didinia; within this range the encystment rate was practically constant and was about 52 per cent. The solutions having hydrogen-ion concentrations between pH 6.4 and 5.0, the acid death limit of the race of Didinium used in the experiments, and between pH 8.4 and 9.6, the alkaline death limit, inhibited encystment, the more injurious solutions producing the greater decrease in encystment rate.The results indicate that the limits of hydrogen-ion concentration within which Didinium can live are practically the same as those found by Crane for Paramecium (approximately pH 5.0 to pH 9.6). They indicate further that concentrations of hydrogen ions which are unfavorable for the growth of didinia do not facilitate encystment and, in general, that changes in hydrogen-ion concentration are of little importance in inducing encystment in Didinium.
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    Journal of Morphology 44 (1927) 
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    Notes: Each step forward in the evolution of new organs or new abilities has made possible further evolution, but at the same time has set up mechanical and physiological conditions that place definite limits on the future possibilities of evolution. The exoskeleton has made possible very definite advances in the evolution of insects, but at the same time has limited their evolution in fully as many other ways.Opportunities in evolution opened up by an exoskeleton are its use as armor, as a skeleton, in the development of wings, as protection against desiccation. It has conditioned small size opening up numerous limited environments, increasing mutability due to short life. It conditions a tracheal system which speeds metabolism, and a variety of specialized mouth parts as well as locating the sense of smell on the surface where it is more effective. Metamorphosis evolved from ecdysis.Limitations imposed by an exoskeleton are small size, simple nervous system due to small size, short life preventing education, inflexible societies due to simple nervous system, cold-bloodedness, clumsy appendages, loss of closed blood system, excretory system reduced to malpighian tubules, poor development of touch and hearing and mosaic vision.
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    Notes: A system of fibers has been found in Dileptus gigas which is probably a neuromotor apparatus. A distinct elongated basal rod, found near the base of the gullet, gives rise to three sets of fibers: (1) a set of heavy fibers radiates out around the funnel-shaped gullet; (2) a pair of heavy fibers pass directly from the basal rod to the band of trichocysts, one on each side, extending to the tip of the proboscis; (3) a set of very delicate branching fibers is distributed over the surface of the organism.This system of fibers is held to be a neuromotor apparatus, because, (1) the general structure and appearance of the fibers suggest a neuromotor function and, (2) there is little or no evidence indicating any other function; and because, (3) the fibers in this system are connected to the most highly specialized structures in Dileptus, and, finally, because, (4) they are similar to structures found in other forms for which a neuromotor function has been experimentally demonstrated.
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    Journal of Morphology 44 (1927), S. i 
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    Journal of Morphology 16 (1900), S. 569-600 
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    Journal of Morphology 17 (1900) 
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    Journal of Morphology 17 (1901), S. 487-514 
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    Journal of Morphology 16 (1900) 
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    Journal of Morphology 40 (1925), S. 299-340 
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    Notes: The course of digestion of an essentially protein food (beef liver) was followed by histological and histochemical methods in Planaria dorotocephala. After an average fasting period of two weeks, the planarians were fed and examined at close time intervals, ranging from one minute to eighty-seven days after feeding. Liver particles are engulfed by the phagocytic cells of the intestine into food vacuoles, where a series of digestive changes occur. The liver particles coalesce into a granular spherule which gradually condenses into a homogeneous and chromatophilic spherule. As the spherule transforms from a granular to a homogeneous condition, it exhibits a gradually increasing affinity for stains, an intensified protein reaction, and a gradual diminution in size. These spherules are protein, since they give positive xanthoproteic and Millon reactions.The homogeneous protein spherules gradually disappear, beginning at about twelve hours after feeding and continuing until the fifth day. During this period fat-droplets form, gradually increasing at the expense of the protein spherules. The protein spherules apparently dissolve into the cytoplasm, furnishing intermediate chemical compounds (tests indicate that a carbohydrate stage does not intervene) from which fat is synthesized. It thus appears that the fat is formed from the protein spherules. The fat thus formed is stored in the phagocytic cells, and remains unchanged in quantity for a fasting period lasting six weeks, after which it gradually diminishes. During the engulfment of the food, the fat decreases markedly, apparently furnishing energy for phagocytic activities. A few of the protein spherules may persist as «protein reserve».
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    Journal of Morphology 40 (1925) 
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    Journal of Morphology 40 (1925), S. 191-233 
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    Notes: Hermaphroditic gonads of Rhabdites elegans can be distinguished in early developmental stages, but oogonia cannot be told from spermatogonia. Certain peculiar males show developmental processes approaching those of hermaphrodites. More eggs are produced by a hermaphrodite than there are sperm with which to fertilize them. Such exhausted hermaphrodites may be mated with males and produce fertile eggs, but convincing evidence of cross-fertilization is lacking. Unmated females of Diplogaster aerivora live longer than mated ones, but no difference was found in the males. Sexual instinct seems to be lacking in males of R. dolichura and R. elegans.Hermaphrodites mated with males produce many more male offspring than by self-fertilization, but these do not exhibit sexual instincts. Females produced by cross-fertilization are not pure females, nor have they been found at all in R. elegans or R. dolichura. After fertilization in these two species, both polar bodies are formed and the pronuclei fuse. Sex is determined by the form of sperm.
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    Journal of Morphology 40 (1925), S. 461-477 
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    Notes: Total splenectomy in the frog means practically complete extirpation of the erythropoietic apparatus. Out of sixty-four operations it was followed by: 1Stimulation of erythropoiesis in the kidney (mesonephros), 35 to 46 per cent.2Stimulation of erythropoiesis in the fat-bodies, 5 per cent; lymphocytopoiesis, 8 per cent.3Stimulation of erythropoiesis in the bone-marrow, 11 per cent.4Formation of a new spleen, 8 per cent.5No perceptible erythropoletic stimulation, 24 per cent. The latter leads to a condition of anemia followed by death within about sixty days.Partial splenectomy leads to spleen regeneration.The life tenure of the frog erythrocyte is approximately 100 days.Total splenectomy experiments indicate that the chief function of the spleen in the frog inheres largely in its lymphocyte content and in the transformation of lymphocytes into erythrocytes.
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    Journal of Morphology 41 (1925) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 157
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    Journal of Morphology 41 (1925), S. 95-157 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This paper represents the third part of a monograph on the development of the lymphatics and lymph hearts of Anura. In this part are considered the origin and development of the subvertebral, lateral, and dorsal lymphatics of the trunk, the caudal lymphatics, and the posterior lymph hearts.The chief lymphatic trunks arise along the veins from a series of small, spindle-shaped, and discontinuous anlagen which subsequently coalesce and thus establish continuity with one another. Lymph plexuses are formed from which emerge the main secondary lymph channels. After such trunks are laid down as indicated, smaller branches and capillaries spring from them by sprouting or extension, due to their actively proliferating endothelium.The posterior lymph hearts ‘crystallize,’ so to speak, from localized areas of the lateral lymphatic plexus. During its reorganization and differentiation, it temporarily separates from the surrounding lymph plexus. Other genetic details, such as the formation of its valves, histogenesis of its walls, number of hearts laid down, etc., are also considered. Twenty-two figures (microphotographs and reconstructions) accompany the text.
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    Journal of Morphology 41 (1925), S. 217-237 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The female reproductive system comprises the ovaries, oviducts, vagina, spermatheca, and spermathecal gland. No colleterial glands are present. The ovaries are paired and each is composed of about eighteen ovarian tubules terminating in a cup-shaped modification of the oviduct - the egg calyx. The epithelial lining of the oviduct is glandular. The spermatheca, a bladder-shaped sac situated dorsal to and between the two ovaries, opens into the vagina near the exterior and is filled with spermatozoa after copulation. Spermatozoa have not been found in any other part of the female. Dorsal to the spermatheca and lying against it is the spermathecal gland. It is connected with the spermatheca by an elongate spermathecal duct.The male reproductive system comprises the testes, vas deferens, accessory glands, and the penis. Each testis is composed of eight to ten cone-shaped follicles, which are composed of a number of cysts so arranged as to appear segmented. All of the cells of each cyst are in approximately the same stage of development. No seminal receptacle is present. Each follicle is connected with the vas deferens by an efferent tube. The paired vas deferens opens into the sac-like intromittant organ or penis. The terminal portion of the penis may be everted, and when everted is conical in shape. At its base is a thin chitinous collar, the margins of which are thickened, forming ridges.
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  • 159
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    Journal of Morphology 41 (1926), S. 283-309 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A study of the development and seasonal changes of the testis in Diemyctylus viridescens shows that accessory testicular lobes arise as follows: Emptying of the caudal zone of the testis at mating is followed by degeneration of the lobules (tubules) of that region, reducing it to a slender cord consisting chiefly of duct system and residual spermatogonia. A regeneration of lobules at the distal end of this cord later gives rise to the second or accessory lobe.Accessory lobes develop in immature males after a similar reduction of the caudal end of the testis through spermatogonial degeneration or an abortive spermatogenesis. The caudal germ-cell cord thus formed is comparable to that resulting from extrusion of spermatozoa, and the development of new lobules at the distal end of this cord gives rise to a second lobe as in adult males. There is no evidence of the formation of a multiple testis by outgrowths from the primary lobe or by the junction of several lobes originally separate.A multiple testis occurs in Urodele species exhibiting two peculiarities of the spermatogenetic pattern: (1) a slow spermatogenetic wave and, (2) delayed regeneration of lobules emptied at mating or by degeneration of their germ cells. The number of lobes is correlated with the age of the individual. In Diemyctylus under 6 cm. the first accessory lobe has not yet developed.
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  • 160
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    Journal of Morphology 41 (1926), S. 347-425 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Amoeba proteus contains a central elongated fluid portion (plasmasol), a rigid layer surrounding this (plasmagel), a thin elastic surface layer (plasmalemma), and a hyaline layer between the plasmagel and the plasmalemma which is fluid at the tip of active pseudopods and in certain other regions.The plasmasol is an emulsion. It consists of a fluid in which various vacuoles and granuoles are suspended. The plasmagel is probably alveolar in structure. It contains the same kinds of substances as the plasmasol, but some of the fluid appears to be gelated so as to form alveoli. The plasmalemma probably consists of interwoven protein fibers and a lipoid which fills the interstices.The plasmasol is probably hypertonic; the plasmagel and the plasmalemma are probably semipermeable. This and other factors result in an excess inflow of water, stretching the plasmagel and the plasmalemma. When a pseudopod is formed, the inner portion of the plasmagel liquefies locally. This produces a local decrease in elastic strength resulting in the formation of a protuberance, a pseudopod. As this is formed there is contraction at the posterior end, resulting in forward flow of the plasmasol and extension of the pseudopod.If the pseudopod is attached, the plasmalemma, being attached to the substratum and to the adjoining plasmagel, slides over the plasmagel above and remains stationary below, rolling movement results. If it is free, the plasmalemma is stretched out with movement in it equal on all sides. If the free pseudopods become attached to the substratum at the tip after they are thus formed, walking movement results.During locomotion of either type, the plasmasol continuously gelates at the tip of the extending pseudopods forming plasmagel, and the plasmagel continuously solates at the posterior end forming plasmasol.Response is due largely to changes in the elastic strength of the plasmagel in the adhesiveness of the plasmalemma and in turgidity.Locomotion in Amoeba verrucosa is in principle the same as it is in Amoeba proteus.
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  • 161
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    Notes: The lips of the animal are modified in relation to the act of passing seeds into the sac. The cheek-sacs are invaginations of the buccal mucosa, and their development as diverticula of the vestibulum oris is associated with the establishment of extrinsic muscles, derived from the platysma. The sac is invested by a coat of striated muscle. The epithelial lining of the sac attains a degree of complexity comparable to the epidermis of the skin, except that a stratum lucidum and stratum granulosum are not developed. No glands are developed in the mucous membrane of the sac.
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  • 162
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    Journal of Morphology 42 (1926), S. 83-109 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The summary of this paper is as follows:1A critical review of the developmental evidence shows that the branchial pouches are formed in cephalocaudal sequence subsequently to the segmentation of the dorsal mesoderm.2The pouches interrupt a continuous sheet of mesoderm to form the branchial arches.3The arches when formed do not correspond topographically to the dorsal somites.4Branchiomerism does not therefore coincide with somitic metamerism.3The branchial structures do not support the theory of head segmentation.3The nervi trigeminus, facialis, glossopharyngeus, and vagus cannot be regarded as segmental nerves.3There is no evidence that branchial pouches or arches have been elided from the series.3The problem of meristic homology is briefly discussed.
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  • 163
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    Journal of Morphology 42 (1926), S. 143-195 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Notes: On the basis of experimental study and observations on the morphology of the legs of spiders, P. Friedrich described a cutting device within the trochanter which would sever that appendage from the body when the leg was stimulated by injury.A reinvestigation of the problem yielded entirely contrary results. Experiments calculated to induce spontaneous amputation through an automatic reflex in the leg were negative on scorpions, harvestmen, and more than a dozen species of spiders. A detailed morphological study of the skeleton and musculature of the arachnid leg showed that no autotomizing device exists, either in the skeleton or in the arrangement of muscles (as is usually supposed). In all species studied, except the scorpion, severance of the leg from the body occurs most readily at some given point. The point at which severance readily occurs is directly correlated with a definite structural weakness in the skeleton and musculature. The spider itself removes an injured leg by grasping the stump with its mouth-parts. No such injured stump falls off spontaneously or without the application of tension. Scorpions do not autotomize appendages under any stimulation, nor do they show any anatomical weakness in the leg skeleton or musculature.Autotomy as an automatic reflex does not exist in the Arachnida.
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  • 164
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    Notes: Sixteen species of the genus Scyllium are here considered and arranged in groups according to the accessory structures present on the claspers as follows:1Canicula, sufflans, ventriosum.2Catulus, stellare, capense, variegatum (var. pantherinus), umbratile.3Burgerii, hispida, bivium, anale, natalense, edwardsii.4Marmoratum, laticeps.The structure of the wall of the oviduct and vagina is demonstrated, and it is shown that the epithelium of both is at first columnar, then ciliated in immature specimens; at maturity that of the vagina further undergoes by transitions a change to stratified. The walls are highly vascular, but contain no special glands.
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  • 165
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    Notes: Amoeba responds to a mechanical shock by a cessation of movement which occurs shortly after the application of the stimulus. The length of the reaction time, the period intervening between application of stimulus and the response, varies inversely with the magnitude of the shock. After stopping Amoeba remains quiescent for a short time, and the length of this period of quiescence varies directly with the magnitude of the shock. A certain amount of time must elapse after a reaction before another can be obtained; during this time the animal reverts to the physiological state which existed prior to the first shock.Partial recovery from the effects of a shock is manifested by a reaction time that is longer than after complete recovery, and a period of quiescence which is shorter. A shock which in itself is too slight to cause a cessation of movement may result in the lack of a response to a heavier one which follows immediately after it, although under other conditions the second shock would have called forth a reaction. If the second shock, however, is made sufficiently heavy, it will bring about a response, despite the effects of the first. This is what would be expected if the reactions take place in accordance with the Weber-Foechner law.
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    Journal of Morphology 42 (1926), S. 523-560 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The different kinds of scales characteristic of the adult sturgeon are described and the facts of ontogenesis which throw light on the history of these structures are presented.The scales of Acipenser are exclusively mesenchymatous and therefore are not morphologically comparable with the placoid scales of elasmobranchs. Salensky ('80) drew erroneous conclusions as a result of failure to study the earlier stages of ontogenesis. Goodrich ('03) has correctly described the ontogenesis of the lepidotrichia.The original form of ganoid scale was that of an elongated rhomb with a longitudinal crest on its external surface. The various forms of scales of the adult sturgeon have been produced by the differentiation of such a scale by change in size, shape, and the fusion of the different elements.The conclusions are extended to the entire family of Acipenseridae.
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    Journal of Morphology 43 (1926), S. 45-54 
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    Notes: The spermatogenesis of the domestic mouse has been studied. There are forty chromosomes in spermatogonia and twenty in primary spermatocytes. The sex chromosomes are of the usual X-Y type. Especial attention has been devoted to the study of chromosome morphology, both in spermatogonia and in primary spermatocytes.
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    Journal of Morphology 43 (1926), S. 119-145 
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    Topics: Biology , Medicine
    Notes: The chromosome number in the domestic fowl is approximately thirty-five or thirty-six. It is difficult to determine the exact number, owing to the smallness of the shortest chromosomes of the complex and also to the tendency of the chromomeres to occasionally appear as discrete chromosomes rather than as parts of a whole.Difficulty experienced in fixing adult testes has prevented a satisfactory demonstration of all stages of spermatogenesis. However, satisfactorily preserved prophases of first spermatocytes have been observed which, together with the large amount of embryonic material available, have made it possible to work out the behavior of the sex-associated chromosomes with reasonable certainty. Measurements of the chromosomes indicate that the longest chromosome in the cell is single in the female and paired in the male. Two classes of eggs are therefore possible - one with and one without this long chromosome, while all the spermatozoa produced are alike in possessing the long chromatic element. The female is therefore heterozygous and the male homozygous in regard to this chromosome which affords a cytological parallel for the genetic evidence of the heterozygosity of the female.
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    Journal of Morphology 44 (1927), S. 467-514 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The eggs of Corella develop in the atrial chamber of the parent at a pH below that of normal sea-water (pH 7.4 ±). When removed to normal sea-water in early stages and under certain other experimental conditions, larval development is more or less inhibited, the tail being most inhibited, the dorsal region somewhat less. The free larval stage may be eliminated and later development and metamorphosis may proceed normally to an advanced stage in the chorion and give rise to normal ascidians. The region most inhibited are, in general, those which possess the highest reducing power, as indicated by KMnO4. Experiments made in the attempt to control development all agree in indicating that the early stages are adjusted to a certain CO2 concentration approximately that of the atrial chamber and presumably near that of the body. Solutions of the same pH may or may not inhibit development according to their CO2 content.The tail, the region of highest reducing power in the embryo during its development, is most inhibited; the dorsal region, the next most rapidly reducing region, is next in degree of inhibition. All differences in reducing power disappear when, or soon after, the animals are killed by other agents before treatment with KMnO4.
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  • 170
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    Notes: This paper contains a description of the appearance and behavior of the chromatin and chief cytoplasmic inclusions in the male germ cells of three species of Belostomatidae.There are eight spermatogonial chromosomes in Lethocerus americanus, twenty-four in Belostoma flumineum, twenty-eight in Benacus griseus. An XY-pair of sex chromosomes occurs in each species. These are identifiable at every step in maturation in Lethocerus, but not in the others. A clear case of heteropycnosis occurs in Benacus. Parasynapsis is believed to be the mode of pairing of chromosomes in all three species. The genesis of three large atelomitic ring tetrads is described in Lethocerus.Chondriosomes are traced from spermatogonia to spermatids without a break in continuity. Spermatogonial chondriosomes are always granular. During the growth period filaments are formed, probably by fusion of granules, and these are sorted out in approximately equal numbers to spermatids. Benacus is especially favorable for studies on chondriosomes. Likewise, Golgi bodies have been traced throughout the process of maturation. They are minute granules in spermatogonia. During the growth period each becomes a flattened plate-like body clearly differentiated into two materials. Dictyosomes are formed by fragmentation in the first prophase, and these are distributed to spermatids where they fuse to form an acroblast in each. Some minor cytoplasmic inclusions are briefly described.
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    Journal of Morphology 45 (1928), S. 121-185 
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    Topics: Biology , Medicine
    Notes: An histological and cytological study of the postembryonic history of the fat-body in Pteronidea ribesi (Scopoli) and Diphadnus appendiculata (Hartig) (both Tenthredinidae) and in Macrocentrus ancylivora Rohwer (Ichneumonidae).The two principal components of the fat-body are the urate-storing excretory cells and the fat-cells.The development and behavior of the excretory cells, especially during the metamorphosis, are described, and in Pteronidea their origin is traced to leucocytes which have become associated with the fat-cells.In the albuminoid inclusions found in the fat-cells two types of substance are distinguished: (a) a basophile material, of nuclear origin, which appears only during the metamorphosis; (b) an acidophile material which appears already during the early larval stages in Macrocentrus, but in Pteronidea is formed only during the metamorphosis and in association with the basophile material. It is thus found that albuminoids formed slowly during larval life may exist from the beginning in the form of acidophile spheres, as occurs in Macrocentrus. But those formed rapidly during the metamorphosis, in all the forms studied, are formed in association with a basophile material derived from the nucleus.The significance of intracellular changes during the metamorphosis is discussed, and the final disposition of the cell inclusions as well as of the fat-cells themselves is described.
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    Journal of Morphology 45 (1928) 
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    Journal of Morphology 45 (1928), S. 399-439 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Topics: Biology , Medicine
    Notes: In this study the problem of the tonsil is considered in anurans. The common toad (family Bufonidae) is used as the type, and representative species of the other families are compared.In each species representative stages beginning before transformation were selected, and the lingual region of each was sectioned. Some thyroid-fed toad tadpoles which had prematurely transformed were examined. The investigations led to the following conclusions: 1Accumulations of lymphocytes occur in all the families except Hylidae.2A pair of tonsils located on either side of the tongue appear before transformation in Bufo, and persist, increasing in size through old age.3This pair has its developmental origin at or near the cephalic end of pouch II. In no other species examined do the tonsils appear as early; in almost all forms the accumulations are inconstant in occurrence, as are also some in Bufo.4The cells of the ‘tonsils’ are lymphocytes of varying sizes. They arise from the mesenchyme; later their accumulations become sites of lymphopoiesis.5This type of lympho-epithelial mass is simple in structure and has a greatly thickened epithelium, due to extensive infiltration by lymphocytes. In the connective tissue the vascular supply is abundant.6The differentiation of lymphocytes may be due to a factor of strain, arising through adjustments made during metamorphosis.7Thyroid-fed toad tadpoles transform, apparently without developing tonsils.
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    Notes: The object of the present study is to determine by quantitative means the rate at which a given number of paramecia can utilize the various amino-acids and to study factors influencing this rate.The method consisted in testing the rate of utilization of isolated and mixtures of amino-acids by a known number of Paramecium caudatum. The amino-acids were used in 0.1 per cent solution and were tested, by the Henriques-Sörensen formol titration method for amino-acids, at the beginning and after the paramecia had lived in these amino-acids for twelve hours. The difference between these two tests, figured in percentage, is considered as the amount utilized by the paramecia. The part played by bacteria was found to be negligible when isolated amino-acids were studied. The influence of temperature on the rate of utilization of the amino-acids has been studied, and the results show a direct relationship between the two.The rate of utilization was decreased by anaesthetics and nitroglycerin. A careful study has been made of a number of isolated amino-acids and the rate of utilization of each discussed in relationship to the others studied. An explanation of why one amino-acid was used more than another amino-acid was sought for, but not found. A detailed discussion of the above points has been presented in the body of the paper.
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    Notes: A chronological investigation of the histological condition of the right ovary of the domestic hen from embryos of nine days' incubation to adults of eighteen months was made. A rudiment of the right ovary is found at all times. Its composition is variable. Sixty-one per cent of the rudimentary ovaries contained medullary tissue only. Thirty-nine per cent had rudiments of cortex, in addition. The occurrence of cortical rudiments in embryonic stages is the probable basis of ovarian follicles found in the rudimentary right ovaries of adults. Primordial germ cells persist in the medullary tissue until three weeks after hatching. They subsequently appear to atrophy. The medullary cords persist through the entire period either as distended tubules or as solid cords of modified epithelial cells. Remnants of the right mesonephros persist as tubules and connect with the gonad by rete tubules. The mesonephric duct maintains a patent lumen.
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    Journal of Morphology 46 (1928), S. 275-315 
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    Notes: Material of the domestic fowl of appropriate ages, ranging from twelve hours' incubation to the adult bird, was prepared for the purpose of studying the production and development of the germ cells.The primordial germ cells arise in the extra-embryonic region anterior to the head fold in the region of the zone of junction during the primitive-streak stage. These germ cells migrate, through the blood stream, to the region of the future gonad, where they develop into the definitive germ plasm.There is no widespread degeneration of the primordial germ cells after their arrival in the gonadal region, nor is there any widespread transformation of somatic cells into definitive germ cells.
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    Journal of Morphology 46 (1928), S. 317-397 
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    Notes: In this study observations are reported upon the morphological differences between the three-toed and two-toed forms of sloths, as well as a comparison made of some aspects between the sloths and the other groups of the Xenarthra. Much attention has been paid to the gross, as well as the histological examination of the viscera, musculature, and the vascular and lymphatic systems.The importance of the correlation of the morphological findings with physiological studies has been emphasized. For example, the probable correlation of the vascular plexuses of the extremities with the postures and muscular activity of the different members of the Xenarthra is discussed. Much new information has been gained concerning the placentation and development of the sloths, as well as concerning the structure of the male and female reproductive tracts.
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    Journal of Morphology 46 (1928), S. 399-430 
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    Notes: The septomaxillary is described in certain anurans and in some of the reptiles in which it occurs. In urodeles and some anurans this bone arises by ossification of the nasal cartilages. Lack of embryological material has prevented the verification of this in the young stages of the reptiles. Adult reptiles have been examined, and in these the bone appears to be and has often been described as a ‘membrane bone.’ It is suggested, however, that the septomaxillary is originally a ‘cartilage bone,’ and that in the reptiles additional membrane bone layers form its main part and obscure its cartilaginous origin. The infolding of the bone in the anurans and reptiles until it lies in close contact with the nasal septum, and thereby loses contact with the external nasal structures, appears to be correlated with the loss of the external nasal muscles. This loss in its turn results from the adoption of terrestrial life and consequent changes in the respiratory mechanism.
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    Journal of Morphology 46 (1928), S. 479-519 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Series of injections of ovarian hormone have been made into normal and ovariectomized immature animals. Injections were made twice daily for twenty-two days. The total dose exceeded 1000 rat units per animal.Effects noted in the living animals were the appearance of reddening and swelling of the ‘sexual skin’ and change of the cell content of the vaginal smear to the interval type of the mature animal. Measurements made at operation, before and after injections, indicated considerable enlargement of both the cervix and body of the uterus. The thymus glands of the injected animals weighed significantly less than those of the controls. Histologic study of the genital tract showed extreme thickening of the vaginal walls, considerable growth of the uterine epithelium and glands, hypertrophy of the muscle layers of the uterus, and advanced differentiation of the epithelium of the uterine tubes.The ovaries of the injected normal animal were smaller and contained fewer primordial and medium-sized follicles than those of the controls. The presence of large numbers of atretic follicles, especially large flattened scars from former relatively well-developed follicles also suggests a harmful effect of this amount of ovarian hormone upon follicular development. Several stages of elimination of ova from polyovular follicles were also observed. There was marked growth in the ducts and an increase in the number of alveoli of the mammary glands.
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    Journal of Morphology 47 (1929), S. 89-133 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: During the telophases each chromosome becomes inclosed in an individual sac or vesicle which, together with its contents, is called a chromosomal vesicle. The vesicular membrane is of cytoplasmic origin, but is formed under the influence of the chromosome and a droplet of karyolymph. A precise numerical correspondence between chromosomes and chromosomal vesicles has not been established, but it is evidence that most, if not all, of the chromosomal vesicles retain their individuality during the resting stage and until after the new chromosomes have been fully formed.The transformation of the telophase chromosome into the reticulum of the resting stage and the manner in which a new chromosome is formed from a portion of this reticulum are described in detail. In the early prophases each developing chromosome is embedded in a sheath or matrix of less deeply basophilic material, which disappears before the middle prophase is reached.The formation of chromosomal vesicles is interpreted as a device for doing more rapidly and effectively, under stress of special circumstances, the work that the nucleus must accomplish during the so-called resting stage.
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  • 181
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  • 182
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    Journal of Morphology 47 (1929), S. 283-333 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The significance of the ultimobranchial body has been the object of a comparative study of the structure in twenty-four species of urodeles. In nineteen of these it has not hitherto been described.Caudal to the last branchial arch, it develops as a thickening and later as an outpushing from the ventral wall of the pharynx. Due to the growth mechanics of the region, it comes to lie obliquely to the pharynx, ventral to it, and dorsal or dorsolateral to the pericardial cavity in its anterior region. It persists throughout life as an epithelioid or epithelial structure, usually of irregular shape, frequently containing vesicles; in some cases it exhibits a considerable amount of secretory activity of variable quality. Except in Amphiuma and Necturus, where it is regularly paired, and in occasional instances in individuals of other species, where it occurs on both sides, it is usually present on the left side only. Its occurrence is constant in all of the species of urodeles for which it has been examined.It is variable in size, form, and position. This, together with the quite inconstant indication of secretory activity, marks it as a structure of little or no physiological significance. ‘Colloid’ is, however, present in some instances, and hence a comparison with the thyroid was considered.
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  • 183
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    Journal of Morphology 47 (1929), S. 435-478 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The perivisceral fluid of Pheretima indica (Horst) contains five types of leucocytes: lymphocytes, monocytes, granulocytes, lamprocytes, and linocytes. The granulocytes differentiate either from free lymphocytes, from peritoneal epithelial cells lining the leucocytopoietic organs, or from lymphocytes (hemocytoblasts) embedded in these organs. The lymphocyte is a hemocytoblast. The eosinophilic granulocyte is the most numerous of the several types of granulocytes. Morphologically and tinctorially, it resembles the eosinophil of fishes. The eosinophil granule is thought to arise either by a ripening of a basophil granule and to pass through a metachromatic phase during this process, or by being formed immediately without such a ripening process in small hemocytoblasts. The stimulus for the excessive production of eosinophils is thought to be the degree of infection of the leucocytopoietic organs by a species of the gregarine, Monocystis.A series of segmentally arranged leucocytopoietic organs is described for the first time in the oligochaetes. These organs are essentially foldings of the septa and offer sacculations in which leucocytopoiesis may take place.A discussion of the possible phylogeny of the hemocytopoietic organs of the invertebrates and vertebrates is given.
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  • 184
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    Journal of Morphology 47 (1929), S. 555-587 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The germ cells of Lebistes are found in cysts; the younger cysts are toward the cortex. Mitochondria and Golgi apparatus are present. During maturation leptotene, bouquet, pachytene, and diakinesis figures are seen. The spermatocyte chromosomes number twenty-three; an X-Y pair is probably present, though the evidence is not conclusive. In spermatid formation the centriole divides to form a rodlet and an axial filament; the nucleus segregates into two materials, one of which is extruded; the remainder first contracts to a cup, comes in contact with the rodlet, then again forms a sphere. The mitochondria are arranged along the proximal part of the axial filament; the sphere flattens and elongates; the rodlet sinks into the head substance and is enfolded by it. The Golgi remnant is sloughed off with the residual cytoplasm, which disappears at the same time the Sertoli cells show an increase in size, suggesting their ingestion of the spermatid remnants. The mature sperm form spermatozeugmen, which are stored in the testicular canal; they are transferred to the female by aid of the modified anal fin.
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  • 185
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    Journal of Morphology 48 (1929), S. 81-103 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A comparative study of the embryology of the rabbit in races of large and small adult size shows a consistently more rapid rate of cell multiplication and of increase in mass in large-race embryos than in those of small race.This more rapid rate of growth is transm tted by and influenced equally by sperm and egg cell, as is shown by the results of reciprocal crosses.Rate of differentiation is independent of rate of growth and unaffected by it. Consequently, embryos of the large race have attained greater size than those of the small race at corresponding stage of differentiation.The fundamental difference in rate of growth is already in evidence in forty-eight-hour embryos and becomes increasingly clear at later stages. Embryos produced by the large race have undergone about one more cell division at forty-eight hours after mating and so are approximately in the thirty-two-cell stage when embryos of the small race are in the sixteen-cell stage.
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  • 186
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    Journal of Morphology 48 (1929) 
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  • 187
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    Journal of Morphology 48 (1929), S. 345-369 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The specimen described presents a typical intersexual condition, in which not only the primary sex characters, but also the accessory sex organs and the secondary sex characters are of a mixed type. Both gonads are ovotestes, in which the ovarian as well as the testicular tissues are morphologically normal, but functionally underdeveloped. Stromal hyperplasia is distinct, especially so in the left gonad; it is probably a functional factor in the generative process of the spermatic tissue.Sex transformation in the intersexual frogs may probably be initiated by an abnormality in sex differentiation or in the genetical constitution of sex, and is only subsequently influenced and modified by environmental action. Absolute dominancy of male sex in the process of sex transformation is questionable. There are indications that in frogs sex transformation may possibly occur in a female direction.
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  • 188
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    Notes: Following the isolation of sperm in the epididymides of male guinea-pigs by the separation of this organ from the testis, the animals were found to remain fertile for periods varying from twenty to thirty-five days, whether they were allowed to mate but once during this period, or five times during the period, or twenty times during the period. Motile sperm were found as long as fifty-nine days after the operation. It was concluded, on the basis of these data, that an aging process which sperm isolated in the epididymis undergo is more important than the number of matings (up to at least nine or ten) in the determination of the period during which sperm motility is retained and in the determination of the period during which fertilizing capacity is retained.If the behavior of sperm isolated in the epididymides can be taken as an indication of the processes of sperm development which occur normally, the epididymis would seem to be an organ in which sperm may at one time be attaining an optimal functional state and may at another time be aging and becoming incapable of functioning. The possible significance of this suggestion for the rǒle of the epididymis in sperm development and for the history of sperm after they leave the testis is discussed.
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  • 189
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    Journal of Morphology 48 (1929), S. 563-584 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: From the structure of statocysts, in the so-called phylogenetic tree of the Cephalopoda, the genus Scaeurgus is to be placed on the same branch as Polypus.Meleagroteuthis has the same number of processes as Watasenia, Enoploteuthis, Abralia, Illex, and Stigmatoteuthis, which have ten processes, but in this genus the structure of statocysts seems to be tolerably well developed. For this reason, the author places this genus as a branch between Abralia and Illex.Those three genera - Gonatus, Sthenoteuthis, and Onychoteuthis - which have eleven processes in a statocyst are to be placed in the same branch as Berryteuthis, Gonatopsis, Ommastrephes, Eucleoteuthis, Moroteuthis, and Symplectoteuthis, which have the same number of processes; of these, Gonatus, which has the most primitive structure of the statocyst, is to be put in the lowest position on that branch; but Sthenoteuthis, which has the same structure of the organ as Ommastrephes, is to be placed on the twig near Ommastrephes; and, lastly, Onychoteuthis has the same organ as that of Moroteuthis, which fact induces the author to set this genus in the position nearest to Moroteuthis.The statocyst of Lolliguncula corresponds exactly to that of Loligo, which has twelve processes; this condition puts this genus on the same twig as Loligo.
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  • 190
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    Notes: This study concerns the voice box or syrinx of the fowl in its possible relation to sex. Although neither hens nor capons crow, ovariotomized hens have been known to do so. It appears that a slight sexual dimporphism exists in the syrinx of certain fowls, and it was thought that a particular form might be essential to the act of crowing. However, no sexual dimorphism is apparent in the syrinx of the Brown Leghorn fowl.The syringeal structures of crowing birds (cocks and ovariotomized females) contain no features which cannot be demonstrated in normal females. Variations were found in the syrinx in this breed and in other breeds, but they had nothing to do with sex. There is a gradual ossification with increasing age and size. It may be concluded that there is no apparent reason why the female fowl should not crow, provided it had the instinct to do so properly developed. The sex hormones, if they act in voice production, must act entirely through the conditioning of the central nervous system.
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    Journal of Morphology 47 (1929), S. 531-553 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The external genitalia and the internal reproductive organs are described. Compound gonads, with a normal development of testicular and ovarian tissues, are found in the positions of the gonads of normal individuals. The genital ducts arise in both types of tissue and serve as common ducts.It is demonstrated microscopically that the male tissue has one and the female tissue two sex chromosomes. The diploid number (or nearly so) is present in both tissues. This is the constitution of the normal sexes.The origin of the gynandromorphs is accounted for by assuming either the theory of dispermy in the binucleated egg or chromosomal elimination.
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  • 192
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    Journal of Morphology 45 (1928), S. 1-45 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Five characters-flatness, elongation, posterior pointedness, delay in division of the body, delay in completion of mitosis-are so distributed among the subdivisions of the Opalinidae as to involve either repeated fortuitous appearances of these characters, a thing not to be believed, or trends resident in the germ plasm. The Ophryoscolecidae show similar distribution of two sets of characters. In the Salpidae there is evidence of trends toward: coiling of the gut; decrease in number, size, regularity, and symmetry of body muscles; simplification and degeneration of the eyes. These qualities appear first in the phylogeny in the chain Salpas, the final phase in the life-cycle. In the course of the evolution the solitary Salpas become more and more modified in the same directions, until, in the most highly modified species, the muscles and eyes are as much modified in the solitary Salpas as in the aggregated. These changes, not disadvantageous but rather adaptive in their beginning in the colonial individuals, are harmful to the solitary Salpas, yet the degeneration is, by precocious development, thrown back onto the earlier phase of the life-cycle, the solitary stage. Precocious development is not purely utilitarian, but may be more fundamental, biological.Evolution is discussed in terms of trends resident in the germ plasm, their origin in connection with mutations, their growth, decrease, disappearance, branching; auto-evolution. Discussion is from the standpoint of the germinal stream, internal factors of evolution being emphasized.
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    Journal of Morphology 47 (1929), S. 227-259 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A histological study was made in the guinea-pig of the distribution of melanin pigment in the normal dark-eye and in various mutant types, such as the brown type of dark-eye (b), light and dark salmon-eye (sm), dark red-eye (crB), brown red-eye (crb), albino (ca), pink-eye (p), and pseudopink-eye (P sm b). The genes which affect pigmentation in the eye have definite qualitative or quantitative effects on the production of melanin. Eyes of the genetic constitution ca, p, or sm possess very little pigment. But eyes which have the dominant allelomorphs of these genes, C, P, or Sm, have a large amount of pigment. The non-yellow gene, cr, and the brown gene, b, cause the deeper retinal layer, which is normally intensely pigmented throughout its entirety, to become less pigmented. There is a corresponding reduction in the melanin in the pigmented areas of the iris and choroid. In all types of eyes with reduced pigment, such as the albino, pink-eye, and pseudopink-eye, pigment persists in the deeper retinal layer even when it is almost absent from other regions. Moreover, in all eyes which have less pigment than the normal type there is a decided tendency for chromatophores to be located adjacent to blood vessels. This tendency is outstanding in the iris and quite noticeable in the choroid.
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    Journal of Morphology 47 (1929), S. 335-413 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An attempt is made to define lips, and on Danforth's interpretation of homology, homologous lips are found at certain stages of development in some representatives of all classes of vertebrates. The primary lips characteristic of selachians, after the maxillary and premaxillary bones have developed within the territory of the upper lip (toadfish, cod), may disappear (trout, Spelerpes), accompanied by a forward migration of the lower jaw. The secondary lips of higher forms are first indicated in certain teleosts and amphibians. Lips vary in structure to accord with their physiological functions, whether sensory, prehensile, or adhesive. Lips of the cod are highly sensory; those of the tadpole and of grazing animals, in different ways, are notably prehensile; the lips of petromyzon and the vampire, having abundant villi, are most effectively adhesive. Therefore, the smaller villi of the lips of suckling animals are presumably for tight adhesion to the nipple. The opossum and rat, however, nurse before their lips have developed. No free macroscopic villi are found on human lips, but there is a zone of thick epithelium tending to form villi. Such a zone is shown to be a widespread feature of vertebrate lips.
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  • 195
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    Notes: During the past few years many functions have been assigned to the epididymis which have been supplementary to its rǒle in sperm storage and to its secretory activity. One of these, the suggestion that sperm attain full maturity and are strengthened in consequence of some action of its secretion, has been reinvestigated as the first part of a study which is intended to include other aspects of the problem.No evidence in support of the theory was obtained from a series of experiments on various mammals representing a repetition and extension of earlier work. On the contrary, the strengthening and attainment of full spermatozoon maturity would seem to be the outcome of changes which are inherent in the sperm themselves. It is suggested, therefore, until other aspects of the subject can be reinvestigated, that the epididymis is simply a reservoir for sperm in which the processes of sperm development which start while they are still contained in the testis are free to continue because of the favorable environment present in the epididymis.
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    Journal of Morphology 48 (1929), S. 105-121 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Two types of cytoplasmic inclusions, differing in reactions to vital dyes and to osmic fixation and impregnation have been demonstrated in Peranema trichophorum. The mitochondria are rod-like and lie in more or less spiral rows, forming a single layer beneath the periplast. They are stained supravitally with Janus green, but not with neutral red. They may be demonstrated by staining in iron hematoxylin after Mann-Kopsch fixation. They are also blackened in prolonged osmic impregnation, but are bleached readily with hydrogen peroxide.The small spherical osmiophilic inclusions are scattered in distribution, although sometimes more numerous in the anterior third of the organism. These bodies are stainable supravitally with neutral red, neutral violet, and brilliant cresyl blue. They are densely blackened in osmic impregnation, and are not bleached in the usual treatment with hydrogen peroxide. They are not however, demonstrated by Mann-Kopsch fixation and iron hematoxylin. After being stained supravitally with neutral red, these inclusions may be blackened under direct observation by exposure to osmic vapor in hanging-drop preparations.
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    Journal of Morphology 48 (1929), S. 173-251 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The purpose of this study was to determine whether structural elements of the cytoplasm can be demonstrated to be specifically concerned with certain physiological processes that are experimentally induced in tissue at the time of fixation. The methods for bringing into view variations in cell structure correlated with cell activity were as follows: the increase of the process of secretion by subcutaneous injection into the living animal of phenol red and of urea; the increase of the process of reabsorption by injection of glucose: the inhibition of reabsorption of sugar by injection of phlorhizin.Variations of the same magnitude in the form, position, quantity, and interrelationships of granules, globules, chondriosomes, and Golgi substance were found to occur in normal untreated animals and in those treated experimentally. Some evidence was found that in cells of the proximal tubule a characteristic form of Golgi body appears which seems to be correlated with increased reabsorption of sugar.
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  • 199
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    Notes: A histological study has been made of a series of Phrynosoma testes taken at frequent intervals throughout the year. As it was important to differentiate between the amount of interstitial tissue and the number of cells, it was necessary to take into account the percentage of tissue, total testis volume and the number of cells per unit volume.The results show a definite interstitial-cell cycle. Volume of interstitial tissue and size of individual cells are greatest during the breeding season, but the interstitial-cell number is then minimal. The maximal number of interstitial cells occurs after the close of the breeding season. There is, apparently, no reversion of interstitial cells to a connective-tissue-cell type.The above changes are correlated with the spermatogenetic cycle and with increases in total tubule length, in tubule diameter, and in testis volume. The time in which important changes in ovarian volume occur and at which ovulation takes place is coincident with the testicular changes.Interstitial cells have been stated to have no endocrine function for the reason that their number is at a minimum during the breeding season. If, however, interstitial tissue is responsible for sex characters and activity, the present work would indicate that it is the volume of tissue, and not the number of cells, that is important.
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    Journal of Morphology 48 (1929), S. 611-625 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: By the use of a new technique it was possible to demonstrate the Golgi granules about the idiosome of spermatids and in the so-called Golgi bead of the mature spermatozoon. Other osmiophile granules in the cytoplasm, assumed to be Golgi granules, aggregate during metamorphosis into groups which formed neutral fat spheres, giving stains with both sudan III and scharlach R. Evidence is adduced supporting the view that the Golgi apparatus is a lipin and probably a phospholipin. The fat spheres produced are probably a result of fatty degeneration in the residual body of the spermatid. They may also serve as the source of lipin in Popa's ‘Lipo-gel’ phenomenon.
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