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  • Articles  (39)
  • Biochemistry and Biotechnology  (39)
  • 1955-1959  (39)
  • 11
    Electronic Resource
    Electronic Resource
    Batch centrifuge scale-up for virus processing (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 207-215 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The mathematical relationship between the throughput of a batch centrifuge and its mechanical characteristics has been applied to the scale-up of one of the steps in virus processing for possible application to the production of vaccines and similar antigens. Slurries that contained a test virus were purified in a laboratory centrifuge. The Q/Σ relationship, developed by Ambler, was used to predict the operating conditions of a larger centrifuge in order to achieve the same clarification of slurry as that produced in the laboratory centrifuge. A correlation between conditions of centrifugation and amount of virus removed from the feed to the centrifuge shows that there is a well-defined Q/Σ value at which significant concentrations of the test virus are sedimented.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010207
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  • 12
    Electronic Resource
    Electronic Resource
    The interaction of unsaturated and saturated steroids in the production of Δ1,4-androstadienedione with Fusarium caucasicum (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 239-242 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010210
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  • 13
    Electronic Resource
    Electronic Resource
    Cholesterol by David Kritchevsky. John Wiley & Sons Inc., 1959, xi + 291 pp. 78/- (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 243-243 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010211
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  • 14
    Electronic Resource
    Electronic Resource
    An automatic apparatus for extraction of cell suspensions by repeated freezing and thawing (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 229-238 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An account is given of the construction and operation of a fully automatic apparatus in which the material is kept under agitation in a closed container alternately surrounded by hot and cold alcohol supplied from separate baths. The apparatus has the following applications in microbiology and cell physiology: (1)Extraction of micro-organisms and cell tissues by repeated freezing (F) and thawing (T) at variable temperatures and lengths of FT cycle. Extraction can be controlled automatically or manually. As a practical example of the use of the apparatus a description is given of the extraction of living virulent S. typhi bacteria.(2)Studies of the reactions of living micro-organisms and tissues under varying physical conditions (e.g. high- and low-temperature resistance experiments, osmotic studies, thermal synchronization of bacterial cultures, etc.).(3)If required, the normal gas mixture in the residual air space can without difficulty be replaced by other gases or mixtures such as CO2 or N2.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010209
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  • 15
    Electronic Resource
    Electronic Resource
    The evaluation of a process for the preparation of co-enzyme A from yeast (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 217-228 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A process is described for the extraction of co-enzyme A from dried yeast. It involves cold water extraction, charcoal adsorption followed by pyridine elution, preparation of an acetone power, co-precipitation as the double cuprous complex with glutathione and removal of the glutathione by means of an ion exchange resin.Improvements over previous processes include use of drum dried yeast plus cold extraction resulting in easy filtration and increase of throughput by a factor of three to five, together with the elimination of the ion exchange step formerly thought necessary to remove zine before the co-precipitation stage. Under the best conditions yields represent 30% recovery of the co-enzyme A in the yeast, the product being 90% pure.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010208
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  • 16
    Electronic Resource
    Electronic Resource
    Masthead (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959) 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010301
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  • 17
    Electronic Resource
    Electronic Resource
    Biochemical preparations, Volume 6; Ed. Carl S. Vestling. New York, John Wiley & Sons, Inc.; London, Chapman & Hall, Ltd. IX and 105 pp. 42/- (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 244-244 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010212
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  • 18
    Electronic Resource
    Electronic Resource
    A highly sensitive katharometer and its application to the measurement of ethylene and other gases of biological importance (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 245-259 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design and construction of a highly sensitive katharometer is described with special reference to the application of the instrument for the precise determination of traces of ethylene by gas chromatography. Factors influencing katharometer sensitivity, and the relative advantages and disadvantages of katharometers as compared with other types of detectors, are discussed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010302
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  • 19
    Electronic Resource
    Electronic Resource
    Separation of acid polyelectrolytes and proteins in cellular extracts - IWork performed under Contract da-91-508-EUC-263 with the European Office of the Chief of Research and Development, U.S. Department of the Army. (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 273-288 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An electrophoretic method for the separation of acid polyelectrolytes from proteins in cell extracts is described.A description of the apparatus is given and some results of the separation of extracts from Bordetella pertussis are included.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010304
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  • 20
    Electronic Resource
    Electronic Resource
    Control of foam during fermentation by the application of ultrasonic energy (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 1 (1959), S. 289-295 
    Details
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An ultrasonic generator was tested at 3 frequencies for its effects on Serratia marcescens during fermentation. The generator controlled foam adequately at 26, 29, and 34 kc/sec at medium aeration rates up to 0·6 ft3/min. Final viable counts were in the same range as those for cultures grown using lard oil as a foam control agent. At 34 kc/sec the final viable counts were slightly higher than those of cultures with lard oil.No harmful effects were evident when large volumes of cultures were exposed to ultrasonic energy during growth.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbmte.390010305
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