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  • Articles  (29,059)
  • Springer Nature  (28,208)
  • Blackwell Publishing Ltd  (851)
  • Springer Science + Business Media
  • American Meteorological Society
  • 1960-1964  (29,059)
  • Biology  (29,059)
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  • Articles  (29,059)
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  • 1
    Electronic Resource
    Electronic Resource
    Studies on Specific Chemicals as Excysting Agents for Pleurotricha lanceolata (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Cysts of the hypotrichous ciliate Pleurotricha lanceolata were placed in dilute aqueous solutions of specific chemicals (carbohydrates, vitamins, L-amino acids, nucleic acids and derivatives, other organic acids and tweens) to assay the effectiveness of these chemicals as excysting agents. It was found that some of the amino acids, deoxyribonucleic acid, ribonucleic acid, and some other organic acids induce excystment at rates equal to or better than the control medium, 0.05% (w/v) aqueous lettuce extract. Preliminary experiments with chelating agents, tri- and tetra-sodium salts of ethylenediaminetetraacetic acid (Versenol 120 and Versene 100), indicate that they successfully initiate excystment, but support it inadequately thereafter so that most of the organisms die within the endocyst.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02638.x
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  • 2
    Electronic Resource
    Electronic Resource
    Utilization of Glucose by Astasia longa (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. A mutant strain of Astasia longa utilized glucose for growth whereas the parent (J) strain did not. The optimal pH for growth of the mutant with glucose (sole carbon source) was near neutrality; the optimal glucose concentration 0.02 M. Cell-free extracts or cell homogenates produced C14O2 when incubated in the presence of C14-labeled glucose. On the other hand, after incubation with C14-labeled glucose, intact parent cells and their respiratory CO2 showed no radioactivity while the mutant-strain cells and CO2 produced were active. Dissimilation of glucose-1-C14 and glucose-6-C14 yielded the same amount of radioactivity in metabolic CO2 in cell-free extracts of both strains. Of five enzymes assayed, hexokinase, phosphoglucomutase, and lactic dehydrogenase were present whereas glucose-6-PO4 dehydrogenase and glucose dehydrogenase were absent in cell homogenates of both strains. Presumably these two strains of A. longa differ in permeability of the plasma membrane. Further tracer and enzyme studies indicated that the Embden-Meyerhof scheme is the principal pathway of glucose catabolism; the hexose mono-phosphate shunt and the direct oxidative pathway were either not operating or quantitatively insignificant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02641.x
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  • 3
    Electronic Resource
    Electronic Resource
    A New Species of Avian Plasmodium (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. A new species of avian malaria parasite is described, for which the name Plasmodium formosanum is proposed. It was observed in the blood of Arboriphila crudigularis, the Formosan hill partridge; other bird species in which it may occur remain unknown, as do the mosquito vectors. It produces very broad, elongate gametocytes, the macrogametocyte usually exhibiting a very large vacuole. The usual number of merozoites is eight. The host cell nucleus typically is not displaced, except laterally by the sexual forms. Only the stages occurring in the blood have yet been seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02642.x
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  • 4
    Electronic Resource
    Electronic Resource
    Biochemical Studies on Irradiated Protozoa. I. Effect of Metabolites on the Respiration of X-Irradiated Tetrahymena pyriformis (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. When Tetrahymena suspended in water were given increasing doses of radiation, oxygen consumption decreased with increasing dose, reaching 60–90% at 600,000 r. Cells irradiated in 0.07 M phosphate buffer, pH 7.4, showed no significant decrease in oxygen consumption even at 600,000 r. The decrease in respiration observed on irradiation of Tetrahymena pyriformis W in water with 300,000 r of X-radiation was prevented by addition of pyruvate or acetate during or immediately after irradiation. Pyruvate stimulated the respiration of the X-irradiated cells, particularly at 10 and 60 min post-irradiation.Lactate markedly stimulated the respiration of control suspensions of Tetrahymena cells and oxidation of lactate by cells irradiated with 300,000 r was increased by 20 to 100%, depending on the concentration of lactate and the time after irradiation. Pyruvate was considerably more effective than lactate in increasing O2 uptake of X-irradiated cells, particularly at 10 min post-irradiation. Thioctic acid affected neither the respiration of control or X-irradiated Tetrahymena nor the oxidation of pyruvate.The growth lag of Tetrahymena increased proportionately with increasing radiation dose; no cells survived 600,000 r. The presence of metabolites during irradiation did not affect the lag period or subsequent growth rates. The effects observed were discussed in terms of an alteration of the permeability of Tetrahymena after irradiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02596.x
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  • 5
    Electronic Resource
    Electronic Resource
    Carotenoid Production by Streptomycin-Bleached Euglena (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Streptomycin-bleached Euglena gracilis, strain Z, was cultivated under conditions which yielded good growth rates and high cell densities. Dividing cells produced only small amounts of carotenoid. After the cessation of cell division the carotenoid content of the cells increased rapidly. During the major period of carotenoid synthesis the cell number remained unchanged but the packed cell volume decreased. Some similar observations on carotenoid production by normal dark-grown Euglena are noted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02598.x
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  • 6
    Electronic Resource
    Electronic Resource
    Le Genre Trimyema Lackey et les Caracteristiques Structurales des Ciliés Trichostomatida (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The longitudinal kineties of the holotrich ciliate Trimyema marinum each bear only three widely spaced cilia; but these cilia are located at identical horizontal levels so that they comprise three helicoidal ciliary bands running around the anterior pole of the organism. The two kineties whose cilia are found most anteriorad, located in a right dorso-lateral position on the body, are exceptional, however: they are prolonged anteriorly, bearing numerous short additional cilia.These two “polykineties” wind clockwise around a kind of apical disc; their distal extremities continue to curve, dipping into a “prebuccal invagination” in which they may be said to constitute a vestibular ciliature.In spite of its rather unique characteristics, the curious anatomy of this ciliate does conform to the organizational type recognized for holotrichs of the order Trichostomatida.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02597.x
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  • 7
    Electronic Resource
    Electronic Resource
    Partial Characterization of Hexokinase from Euglena gracilis var. bacillaris (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. A partially purified hexokinase extracted from Euglena had the following properties: 1) pH optimum 8.0–8.5; 2) an absolute requirement for Mg++ and adenosine triphosphate with Mn++, Co++, Zn++, and Ca++ less active or inactive under the test conditions. Inosine triphosphate could not be substituted for adenosine triphosphate; 3) an apparent Km (glucose) of 5 × 10−4 M; 4) the enzyme was not inhibited by the usual sulfhydryl agents, but was inhibited 24% by adenosine diphosphate. The extract phosphorylated fructose and mannose. It was not clear whether a separate enzyme phosphorylated fructose. Relative rates of phosphorylation, as determined by labile phosphate analyses, were: glucose, 1.00; fructose, 1.19; mannose, 0.56. The partially purified extract also contained phosphohexose isomerase activity; 6-phosphogluconic acid dehydrogenase was absent. Euglena hexokinase is apparently localized in the cytoplasmic or small-particle fraction of the cell. In most respects the enzyme seems to resemble the well-characterized yeast hexokinase rather than the muscle enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02605.x
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  • 8
    Electronic Resource
    Electronic Resource
    La Synthèse de l'Acide Désoxyribonucléique au Cours du Cycle de Division de Trypanosoma mega (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Tritiated thymidine and autoradiographic methods were used to investigate the cyclic DNA synthesis in the culture form of Trypanosoma mega. It was found that the mean generation time of 18.9 hours comprises four successive periods: G1, S, G2 and D. The interphase lasts through the first three. S is the phase of DNA synthesis of both the nucleus and the kinetonucleus (kinetoplast). The cell divides during D, beginning with the division of the kinetonucleus. The respective durations of G1, S, G2 and D are 8.5, 7, 2 and 1.4 hours. The close time relationship between the two DNA synthesizing bodies is considered as bringing support to the old theory of the Binucleates and the possible genetic function of the kinetonucleus is suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02607.x
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  • 9
    Electronic Resource
    Electronic Resource
    Production of a Vitamin B12 Compound by Tetrahymenids (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Production of a “vitamin B22-like compound” by Tetrahymena pyriformis mating type II, variety 1, and 3 other tetrahymenids was demonstrated. The compound produced by T. pyriformis was nutritionally active for Escherichia coli and Lactobacillus leichmannii and inert for Ochromonas malhamensis and Euglena gracilis, despite use of a variety of the standard methods for liberating bound B12. By dry-weight it is present in 1 part in 30 million. Its chromatographic and electrophoretic properties are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02608.x
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  • 10
    Electronic Resource
    Electronic Resource
    Effect of Type of Carbohydrate on Growth and Protein Synthesis by Tetrahymena pyriformis (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. In chemically defined media at carbohydrate concentrations ≧ 0.5% (w/v) Tetrahymena pyriformis W multiplied more rapidly, developed larger cells, and achieved greater growth as measured by optical density when carbohydrate was provided as dextrin rather than glucose. In media containing 0.3 mg/ml of amino acid nitrogen, growth increased with glucose concentration from 0.1 to 1%, did not change significantly to 3%, and was sharply inhibited at higher glucose levels. With dextrin, maximum growth paralleled carbohydrate concentration from 0.1 to 3%. At higher N levels the inhibitory concentration of glucose was lowered, but growth in dextrin media was not affected except at N concentrations that were inhibitory independent of carbohydrate source. At 1% carbohydrate levels, total cell protein per ml of culture was 60% greater, protein per cell approximately 50% greater, and cells were 1.5 to 2 times larger in media with dextrin than with glucose. Comparable differences in protein synthesis were observed at 2% carbohydrate levels and efficiency of conversion of substrate-N to protein-N was greater in the medium with dextrin than glucose.Growth as measured by optical density in media with 0.3 mg/ml of N and 1 or 2% (w/v) of dextrin was not significantly reduced by the simultaneous presence of 1 or 2% glucose. This observation appeared to negate osmotic pressure as an explanation of reduced growth in the presence of glucose. At higher osmolar concentrations osmotic pressure appeared to be a major determinant of overall growth but not of cell size.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02609.x
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