ALBERT

Description: Anaerobic methane-oxidizing microbial communities in sediments at cold methane seeps are important factors in controlling methane emission to the ocean and atmosphere. Here, we investigated the distribution and carbon isotopic signature of specific biomarkers derived from anaerobic methanotrophic archaea (ANME groups) and sulphate-reducing bacteria (SRB) responsible for the anaerobic oxidation of methane (AOM) at different cold seep provinces of Hydrate Ridge, Cascadia margin. The special focus was on their relation to in situ cell abundances and methane turnover. In general, maxima in biomarker abundances and minima in carbon isotope signatures correlated with maxima in AOM and sulphate reduction as well as with consortium biomass. We found ANME-2a/DSS aggregates associated with high abundances of sn-2,3-di-O-isoprenoidal glycerol ethers (archaeol, sn-2-hydroxyarchaeol) and specific bacterial fatty acids (C16:1ω5c, cyC17:0ω5,6) as well as with high methane fluxes (Beggiatoa site). The low to medium flux site (Calyptogena field) was dominated by ANME-2c/DSS aggregates and contained less of both compound classes but more of AOM-related glycerol dialkyl glycerol tetraethers (GDGTs). ANME-1 archaea dominated deeper sediment horizons at the Calyptogena field where sn-1,2-di-O-alkyl glycerol ethers (DAGEs), archaeol, methyl-branched fatty acids (ai-C15:0, i-C16:0, ai-C17:0), and diagnostic GDGTs were prevailing. AOM-specific bacterial and archaeal biomarkers in these sediment strata generally revealed very similar δ13C-values of around −100. In ANME-2-dominated sediment sections, archaeal biomarkers were even more 13C-depleted (down to −120), whereas bacterial biomarkers were found to be likewise 13C-depleted as in ANME-1-dominated sediment layers (δ13C: −100). The zero flux site (Acharax field), containing only a few numbers of ANME-2/DSS aggregates, however, provided no specific biomarker pattern. Deeper sediment sections (below 20 cm sediment depth) from Beggiatoa covered areas which included solid layers of methane gas hydrates contained ANME-2/DSS typical biomarkers showing subsurface peaks combined with negative shifts in carbon isotopic compositions. The maxima were detected just above the hydrate layers, indicating that methane stored in the hydrates may be available for the microbial community. The observed variations in biomarker abundances and 13C-depletions are indicative of multiple environmental and physiological factors selecting for different AOM consortia (ANME-2a/DSS, ANME-2c/DSS, ANME-1) along horizontal and vertical gradients of cold seep settings.

Description: CORONA images have been used for the mapping of periglacial features on the Bykovsky Peninsula and adjacent Khorogor Valley in northeast Siberia. Features, mapped and analysed within a geographical information system, include thermokarst depressions, thermo-erosional valleys, thermo-erosional cirques, thermokarst lakes, thermokarst lagoons and pingos. More than 50% of the area is strongly influenced by thermally-induced subsidence. Thermokarst in the area is probably less active today than in the early-middle Holocene.

Description: Samples from three hot springs (Alla, Seya and Garga) located in the northeastern part of Baikal rift zone (Buryat Republic, Russia) were screened for the presence of thermophilic nitrifying bacteria. Enrichment cultures were obtained solely from the Garga spring characterized by slightly alkaline water (pH 7.9) and an outlet temperature of 75°C. The enrichment cultures of the ammonia- and nitrite oxidizers grew at temperature ranges of 27–55 and 40–60°C, respectively. The temperature optimum was approximately 50°C for both groups and thus they can be designated as moderate thermophiles. Ammonia oxidizers were identified with classical and immunological techniques. Representatives of the genus Nitrosomonas and Nitrosospira-like bacteria with characteristic vibroid morphology were detected. The latter were characterized by an enlarged periplasmic space, which has not been previously observed in ammonia oxidizers. Electron microscopy, denaturing gradient gel electrophoresis analyses and partial 16S rRNA gene sequencing provided evidence that the nitrite oxidizers were members of the genus Nitrospira.

Description: For the understanding and assessment of recent and future carbon dynamics of arctic permafrost soils the processes of CH4 production and oxidation, the community structure and the quality of dissolved organic matter (DOM) were studied in two soils of a polygonal tundra. Activities of methanogens and methanotrophs differed significantly in their rates and distribution patterns among the two investigated profiles. Community structure analysis showed similarities between both soils for ester-linked phospholipid fatty acids (PLFAs) and differences in the fraction of unsaponifiable PLFAs and phospholipid ether lipids. Furthermore, a shift of the overall composition of the microbiota with depth at both sites was indicated by an increasing portion of iso- and anteiso-branched fatty acids related to the amount of straight-chain fatty acids. Although permafrost soils represent a large carbon pool, it was shown that the reduced quality of organic matter leads to a substrate limitation of the microbial metabolism. It can be concluded from our and previous findings first that microbial communities in the active layer of an Arctic polygon tundra are composed by members of all three domains of life, with a total biomass comparable to temperate soil ecosystems, and second that these microorganisms are well adapted to the extreme temperature gradient of their environment.

Description: Induced breeding of climbing perch, Anabas testudineus was conducted by synthetic hormone Wova-FH in the intensity level of 0.1, 0.2 and 0.3 mL kg−1 of body weight respectively. The brooders were injected one time and left to spawn in the spawning hapa in the sex ratio between male and female as 2:1. It was found that at all the intensity level hormone Wova-FH could enhance the fishes to breed and lay eggs whereas no breeding was observed in control set. The spawning time, quantity of the brooder spawn, fertilization rate, hatching rate and survival rate were quantified in each set of experiment. The egg output/female was significantly higher in 0.3 mL in comparison with 0.1 and 0.2 mL kg−1 of body weight. The statistical analysis showed significant (P≤0.05) effect between hormone dose on fertilization rate, egg output and hatching rate. The present experiment suggests that Wova-FH at the dose of 0.3 mL kg−1 body weight of fish is more effective which might be considered for raising captive population.

Description: Detailed studies of the behavior and location of free-ranging animals can be considerably enhanced with the use of animal-mounted devices. A few devices, such as data loggers, have to be recovered to access the data, whereas satellite tags or radiocollars often are left on the animal after the study period. Recovery of devices, which usually necessitates animal recapture, can be problematic, especially in larger species. This paper presents a new, non-electronic release mechanism, requiring no power source on the animal, that was successfully tested on South American sea lions (Otaria flavescens) in Argentina during the 2003–2004 austral summer. The system has potential for many larger terrestrial or aquatic animals.

Description: The great cormorant Phalacrocorax carbo is thought to have a wettable plumage, providing low body insulation during foraging. Great cormorants should thus be constrained by water temperatures, and show high energy requirements. Surprisingly, this species has one of the widest breeding distributions of all diving birds, and does not require more food than these other species. We explored this apparent paradox by comparing the insulative properties of body plumage in four subspecies of great cormorants ranging from tropical to polar regions. We found that all subspecies retained an insulating air layer in their plumage, which was, however, much thinner than for other species of diving birds. Detailed examination of the plumage showed that each cormorant body feather has a loose, instantaneously wet, outer section and a highly waterproof central portion. This indicates that the plumage of great cormorants is only partly wettable, and that birds maintain a thin layer of air in their plumage. Our findings suggest an unusual morphological-functional adaptation to diving which balances the antagonist constraints of thermoregulation and buoyancy.

Description: 1. Tracer experiments with two diatoms labelled with 13C (Nitzschia palea) and 15N (Fragilaria crotonensis), were conducted to investigate feeding selectivity and interspecific competition between the grazers Asellus aquaticus (Isopoda, Crustacea) and Potamopyrgus antipodarum (Hydrobiidae, Gastropoda). Conventional methods, such as cell counts and estimated biovolume, were used first to detect feeding preferences within the different grazer treatments. 2. The results revealed a significant decline in algal biovolume in all grazer treatments and no indications of active selectivity were observed. In contrast to conventional methods, measurements based on isotope signatures showed strong differences in tracer uptake, thus indicating different degrees of assimilation and digestion by the two grazers. 3. The selectivity index Q, which provides information on the uptake ratio of 13C to 15N, showed a significant time effect for both grazer species and a significant difference between single- and mixed-grazer treatments for P. antipodarum. Thus, this technique enabled the direct quantification of the uptake by grazers and, therefore, served as an ideal tool for the detection of passive selectivity. 4. Our results indicate a shift in feeding preferences related to between-species competition and a potential divergence of trophic niches when species coexist.

Description: The monthly variation in reproductive cycle, condition factor (fatness), gonad index (GI), ovarian egg diameter and biochemical composition [RNA, DNA content and their ratio RNA/DNA (R/D)] were compared to determine the spawning season of the purplish Washington clam, Saxidomus purpuratus, collected from the west sea of Taean, Korea. All these factors were correlated to the spawning season from May to October. Monthly variation and concentration of the R/D ratio especially represented indicative information about the processes of gonad development and spawning patterns. The increases in R/D ratio in the ovaries corresponded with increases in ovarian egg diameters and the GI during the spawning season. The peak of R/D and RNA content in females was a good indicator of sexual maturation and the DNA content in males. During winter between November and January, the value of fatness, GI, egg size, RNA and DNA content were lower than in other seasons, indicating depletion of energy reserves and lower metabolic demands because of colder temperatures.

Description: A bottom simulating reflector (BSR), which marks the base of the gas hydrate stability zone, has been detected for the first time in seismic data of the Black Sea. The survey area is in the northwestern Black Sea at 44°–45°N and 31.5°–32.5°E. In this paper, seismic wide-angle ocean bottom hydrophone (OBH) and ocean bottom seismometer (OBS) data are investigated with the goal to quantify the gas hydrate and free gas saturation in the sediment. An image of the subsurface is computed from wide-angle data by using Kirchhoff depth migration. The image shows the BSR at 205–270 m depth below the seafloor and six to eight discrete layer boundaries between the seafloor and the BSR. The top of the hydrate layer and the bottom of the gas layer cannot be identified by seismic reflection signals. An analysis of traveltimes and reflection amplitudes leads to 1-D P-wave velocity–depth and density–depth models. An average S-wave velocity of 160 m s−1 between the seafloor and the BSR is determined from the traveltime of the P to S converted wave. The normal incidence PP reflection coefficient at the BSR is −0.11, where the P-wave velocity decreases from 1840 to 1475 m s−1. Velocities and density are used to compute the porosity and the system bulk modulus as a function of depth. The Gassmann equation for porous media is used to derive explicit formulae for the gas hydrate and free gas saturation, which depend on porosity and on the bulk moduli of the dry and saturated sediment. A gas hydrate saturation–depth profile is obtained, which shows that there is 38 ± 10 per cent hydrate in the pore space at the BSR depth, where the porosity is 57 per cent (OBS 24). This value is derived for the case that the gas hydrate does not cement the sediment grains, a model that is supported by the low S-wave velocities. There is 0.9 or 0.1 per cent free gas in the sediment below the BSR, depending on the model for the gas distribution in the sediment. The free gas layer may be more than 100 m thick as a result of a zone of enhanced reflectivity, which can be identified in the subsurface image.

Description: The influence of fluctuating light on diversity and species number of a natural phytoplankton assemblage competing for nutrients was investigated for 48 days under semicontinuous culture conditions. Light conditions were either changed periodically from high (65 μmol photons·m−2·s−1) to low intensity (15 μmol photons·m−2·s−1) at intervals of 1, 3, 6, and 12 days or fixed at constant light conditions of intermediate intensity (40 μmol photons·m−2·s−1). Fluctuating light at intervals of 1–12 days significantly affected phytoplankton diversity. The development of phytoplankton communities differed in treatments with different light regimes. In treatments with long light intervals, species abundance oscillated with the light phases. Differences in the temporal development of phytoplankton communities resulted in hump‐shaped relations between the interval length of the light phases and both species number and diversity index and can be explained by the intermediate disturbance hypothesis. Fluctuating light tends to sustain phytoplankton diversity under nutrient limitation if the light regime changes in the order of several days. This indicates that temporal changes in weather regime are important in preventing competitive exclusion of phytoplankton species in nature.

Description: This paper presents a three‐dimensional (3D) seismic analysis of sediment remobilization and fluid migration in a 2000‐km2 area above the Gjallar Ridge located in the Vøring Basin, offshore Norway. Three distinct types of mounded structures have been identified as resulting from focused fluid/gas migration and associated mud remobilization and intrusion. Type A structures are gently mounded, and we infer that these structures formed because of in situ remobilization of Middle Eocene to Lower–Middle Oligocene fine‐grained sediments in response to fluid and minor sediment injection via deep‐seated normal faults. Type B structures comprise relatively steep‐sided mounds and are restricted to the pre‐Miocene interval. They are often located above narrow zones of discontinuous low‐amplitude reflections resembling gas chimneys. Some of the Type B structures are associated with stacked amplitude anomalies and possible mud volcanoes at the base Pleistocene indicating their long‐term significance as vertical fluid conduits. Type C structures comprise discrete mound features that seem to jack up the Top Palaeocene (Top Brygge) horizon. These are similar to hydrothermal mounds found elsewhere on the Norwegian Margin and associated with igneous sill intrusion during North Atlantic breakup. This study highlights the utility of 3D seismic data for mapping of fluid and sediment mobilization through time over large basinal areas.

Description: Herbivory is particularly intense in tropical benthic communities, suggesting preference of constitutive, rather than inducible, anti-herbivory defense. The objective of the study was to examine whether anti-herbivore defenses in the red alga Hypnea pannosa J. Agardh and the brown algae Sargassum asperifolium Hering and G. Martens ex J. Agardh and Cystoseira myrica (S.G. Gmelin) C. Agardh could be induced and subsequently reduced in response to grazing by the amphipod Cymadusa filosa Savigny. During a 14-day treatment phase, algae were exposed to amphipod grazing or were left ungrazed (control). Subsequently, one subset of algae was used in feeding assays, whereas another was cultivated for additional 14 days without consumers (recovery phase). At the end of each phase, bioassays were conducted to detect defensive traits in terms of differences in consumption rates of grazed and control pieces of live algae and agar-based food containing nonpolar algal extracts. Consumption of grazed live S. asperifolium and H. pannosa specimens was lower than of control algae. Furthermore, nonpolar extracts of grazed S. asperifolium and C. myrica were less preferred than those from control algae. Defensive responses were exclusively detected after the treatment phase, although strong preference of ungrazed H. pannosa and C. myrica over grazed conspecifics continued throughout the recovery phase. These findings suggest that phenotypic plasticity in anti-herbivory defense of marine macroalgae 1) might be more common than previously shown, 2) could be switched on and off within 2 weeks, and 3) can be found in nonpolar algal extracts.

Description: River discharge of Ob and Yenisei to the Kara Sea is highly variable on seasonal and interannual time scales. River water dominates the shallow bottom water near the river mouths, making it warmer and less saline but seasonally and interannually more changeable than bottom water on the deeper shelf. This hydrographic pattern shows up in measurements and modelling, and in stable isotope records (δ18 O, δ13 C) along the growth axis of bivalve shells and in multiple analyses of single benthic foraminiferal shells. Average isotope ratios increase, but sample-internal variability decreases with water depth and distance from river mouths. However, isotope records of bivalves and foraminifera of a sediment core from a former submarine channel of Yenisei River reveal a different pattern. The retreat of the river mouth from this site due to early Holocene sea level rise led to increasing average isotope values up core, but not to the expected decrease of the in-sample isotope variability. Southward advection of cold saline water along the palaeo-river channel probably obscured the hydrographic variability during the early Holocene. Later, when sediment filled the channel, the hydrographic variability at the core location remained low, because the shallowing proceeded synchronously with the retreat of the river mouth.

Description: A total of 58 extracts of different polarity were prepared from various organs of 16 species of Turkish plants and screened for their antitrypanosomal, antileishmanial and antiplasmodial activities. No significant activity was observed against Trypanosoma cruzi, whereas many extracts showed appreciable trypanocidal potential against T. brucei rhodesiense, with the CHCl3-soluble portion of Phlomis kurdica being the most active (IC50 2.7 µg[sol ]mL). Almost all extracts, particularly the CHCl3 phases, exhibited growth inhibition activity against Leishmania donovani amastigotes. The CHCl3-solubles of Putoria calabrica roots (IC50 1.9 µg[sol ]mL), Wendlandia ligustroides leaves (IC50 2.1 µg[sol ]mL) and Rhododendronluteum leaves (IC50 2.3 µg[sol ]mL) displayed the highest leishmanicidal potential. The majority of the extracts also possessed antiplasmodial activity against the multi-drug resistant K1 Plasmodium falciparum strain. The most potent antiplasmodial activity was observed with the CHCl3 extracts of Phlomis kurdica (IC50 1.5 µg[sol ]mL), P. leucophracta (IC50 1.6 µg[sol ]mL), Scrophularia cryptophila (IC50 1.8 µg[sol ]mL), Morina persica (IC50 1.9 µg[sol ]mL) and the aqueous root extract of Asperula nitida subsp. subcapitellata (IC50 1.6 µg[sol ]mL). Twenty-one extracts with significant antimalarial activity (IC50 〈 5 µg[sol ]mL) were also tested for their ability to inhibit the purified enoyl-ACP reductase (FabI), a crucial enzyme in the fatty acid biosynthesis of P. falciparum. The CHCl3 extract of Rhododendronungernii leaves (IC50 10 µg[sol ]mL) and the H2O-soluble portion of Rhododendronsmirnovii leaves (IC50 0.4 µg[sol ]mL) strongly inhibited the FabI enzyme. The preliminary data indicate that some (poly)phenolic compounds are responsible for the FabI inhibition potential of these extracts. The presented work reports for the first time the antiprotozoal activity of nine different genera as well as a target specific antimalarial screening for the identification of P. falciparum FabI inhibitors from medicinal plant extracts.

Description: Loligo vulgaris is a commercially important squid throughout the Mediterranean region and is a candidate species in biomedical and aquaculture research. Some loligo species (L. opalescens, L. forbesi, Sepiteuthis lessoniana) have now been cultured through some successive generations in closed, recirculating seawater systems. The effects of salinity on hatching European Squid (L. vulgaris Lamarck, 1798) eggs were investigated during November 2004. The egg capsules were incubated directly in salinity of 32, 34, 36, 38, 40, 42 and 37 g L−1 (control group) at 19.8°C (SD 1.2°C), and a photoperiodicity of 12 h light:12 h dark for 16–23 days before hatching. In all treatments, the eggs were developed and hatched normally after 16–22 days at 32 g L−1, 17–22 days at 34, 18–21 days at 42 g L−1, 18–22 days at 36 and 40 g L−1, 19–22 days at 37 g L−1 and 19–23 h at 38 g L−1. In the experiments, the highest hatching rate and hatching success (HS) of the eggs were obtained at 38 g L−1 (hatching rate: 100% (SD 0%) and HS: 96.7% (SD 3.5%)) and the lowest hatching rate at 42 g L−1 (hatching rate: 3% (SD 6%) and HS: 0%). Dorsal mantle lengths (DML) of new hatchlings ranged from 2.08 to 2.80 mm. The present study showed that salinity affects the hatching rate and HS of eggs and first hatching time and DML of paralarvae in L. vulgaris. The squid eggs at stage 11 (I) can tolerate 5 g L−1 reduction and 3 g L−1 increase in salinity.

Description: The Galápagos volcanic province (GVP) includes several aseismic ridges resulting from the interaction between the Galápagos hotspot (GHS) and the Cocos–Nazca spreading centre (CNSC). The most prominent are the Cocos, Carnegie and Malpelo ridges. In this work, we investigate the seismic structure of the Carnegie ridge along two profiles acquired during the South American Lithospheric Transects Across Volcanic Ridges (SALIERI) 2001 experiment. Maximum crustal thickness is ∼19 km in the central Carnegie profile, located at ∼85°W over a 19–20 Myr old oceanic crust, and only ∼13 km in the eastern Carnegie profile, located at ∼82°W over a 11–12 Myr old oceanic crust. The crustal velocity models are subsequently compared with those obtained in a previous work along three other profiles over the Cocos and Malpelo ridges, two of which are located at the conjugate positions of the Carnegie ones. Oceanic layer 2 thickness is quite uniform along the five profiles regardless of the total crustal thickness variations, hence crustal thickening is mainly accommodated by layer 3. Lower crustal velocities are systematically lower where the crust is thicker, thus contrary to what would be expected from melting of a hotter than normal mantle. The velocity-derived crustal density models account for the gravity and depth anomalies considering uniform and normal mantle densities (3300 kg m−3), which confirms that velocity models are consistent with gravity and topography data, and indicates that the ridges are isostatically compensated at the base of the crust. Finally, a two-dimensional (2-D) steady-state mantle melting model is developed and used to illustrate that the crust of the ridges does not seem to be the product of anomalous mantle temperatures, even if hydrous melting coupled with vigorous subsolidus upwelling is considered in the model. In contrast, we show that upwelling of a normal temperature but fertile mantle source that may result from recycling of oceanic crust prior to melting, accounts more easily for the estimated seismic structure as well as for isotopic, trace element and major element patterns of the GVP basalts.

Description: The two agar-producing red algae, Gracilaria chilensis C. J. Bird, McLachlan & E. C. Oliveira and Gracilaria conferta (Schousboe ex Montagne) Montagne, responded with hydrogen peroxide (H2O2) release when agar oligosaccharides were added to the medium. In G. conferta, a transient release was observed, followed by a refractory state of 6 h. This response was sensitive to chemical inhibitors of NADPH oxidase, protein kinases, protein phosphatases, and calcium translocation in the cell, whereas it was insensitive to inhibitors of metalloenzymes. Transmission electron microscopic observations of the H2O2-dependent formation of cerium peroxide from cerium chloride indicated oxygen activation at the plasma membrane of G. conferta. A putative system, consisting of a receptor specific to agar oligosaccharides and a plasma membrane-located NADPH oxidase, appears to be responsible for the release of H2O2 in G. conferta. Subcellular examination of G. chilensis showed that the H2O2 release was located in the cell wall. It was sensitive to inhibitors of metalloenzymes and flavoenzymes, and no refractory state was observed. The release was correlated with accumulation of an aldehyde in the algal medium, suggesting that an agar oligosaccharide oxidase is present in the apoplast of G. chilensis. The presence of this enzyme could also be demonstrated by polyacrylamide electrophoresis under nondenaturating conditions and proven to be variable. Cultivation of G. chilensis at 16 to 17°C resulted in significantly stronger expression of agar oligosaccharide oxidase than cultivation at 12°C, which indicates that the enzyme is used under conditions that generally favor microbial agar macerating activity.

Description: We investigated the effects of ircinin-1, a lipid compound (a C25 sesterterpene tetronic acid) isolated from marine sponges (Sarcotragus sp.), on the modulation of cell cycle and induction of apoptosis in SK-MEL-2 human skin cancer cells (mutant p53). Ircinin-1 treatment on SK-MEL-2 cells resulted in a dose-dependent inhibition of cell growth and induced apoptotic cell death. Flow cytometric analysis revealed that ircinin-1 resulted in G1 arrest in cell cycle progression which was associated with a marked decrease in the protein expression of D-type cyclins and their activating partners Cdk 4 and 6 with concomitant inductions of p21WAF1/CIP1 and p27KIP1. The induction of p21WAF1/CIP1 appears to be transcriptionally upregulated and is p53-independent. In addition, ircinin-1 suppressed the phosphorylation of pRb protein and increased the co-association of pRb or proliferating cell nuclear antigen (PCNA) with p21WAF1/CIP1 in these cells. Ircinin-1 treatment also resulted in induction of apoptosis as determined by morphological changes, DNA fragmentation, alternated ratio of Bax/Bcl-2, cleavages of poly(ADP-ribose) polymerase and PLC-γ1, and flow cytometric analysis. Ircinin-1 also induced cytochrome c release, cleavage activations of caspase-3 and -9, and upregulation of Fas and Fas-L. Even though the inhibitor of apoptosis protein (IAP) was expressed in ircinin-1-untreated or -treated SK-MEL-2 cells, only the level of cIAP-1, but not XIAP or cIAP-2, was decreased during ircinin-1-induced apoptosis at Western blot and RT-PCR studies. Taken together, these findings suggest that ircinin-1 has strong potential for development as an agent for prevention against skin cancer