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  • Articles  (85)
  • Lepidoptera
  • monoclonal antibody
  • temperature
  • Process Engineering, Biotechnology, Nutrition Technology  (83)
  • Natural Sciences in General  (2)
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  • Articles  (85)
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  • 1
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1991-09-06
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Harris, M K -- New York, N.Y. -- Science. 1991 Sep 6;253(5024):1075.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1953905" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Bacillus thuringiensis/*genetics ; Bacterial Toxins/*genetics ; Diptera ; Larva ; Lepidoptera ; *Pest Control, Biological ; Plants/*genetics ; Pupa
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 2006-07-15
    Description: Despite recent progress in understanding mechanisms of tree species coexistence in tropical forests, a simple explanation for the even more extensive diversity of insects feeding on these plants has been missing. We compared folivorous insects from temperate and tropical trees to test the hypothesis that herbivore species coexistence in more diverse communities could reflect narrow host specificity relative to less diverse communities. Temperate and tropical tree species of comparable phylogenetic distribution supported similar numbers of folivorous insect species, 29.0 +/- 2.2 and 23.5 +/- 1.8 per 100 square meters of foliage, respectively. Host specificity did not differ significantly between community samples, indicating that food resources are not more finely partitioned among folivorous insects in tropical than in temperate forests. These findings suggest that the latitudinal gradient in insect species richness could be a direct function of plant diversity, which increased sevenfold from our temperate to tropical study sites.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Novotny, Vojtech -- Drozd, Pavel -- Miller, Scott E -- Kulfan, Miroslav -- Janda, Milan -- Basset, Yves -- Weiblen, George D -- New York, N.Y. -- Science. 2006 Aug 25;313(5790):1115-8. Epub 2006 Jul 13.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Entomology, Czech Academy of Sciences and Department of Zoology, University of South Bohemia, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic. novotny@entu.cas.cz〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16840659" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Beetles ; *Biodiversity ; Climate ; Czech Republic ; Feeding Behavior ; *Insects/classification ; Larva ; Lepidoptera ; Papua New Guinea ; Phylogeny ; Population Density ; Slovakia ; *Trees/classification ; *Tropical Climate
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Bioresource Technology 47 (1994), S. 55-57 
    ISSN: 0960-8524
    Keywords: Tomato-processing waste ; hydraulic retention time ; loading rate ; methane ; temperature
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0960-8524
    Keywords: Agglomeration ; X-ray analysis ; eutectic ; liquidus ; potassium oxide ; scanning electron microscopy ; silica ; straw ash ; temperature
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Bioresource Technology 47 (1994), S. 103-106 
    ISSN: 0960-8524
    Keywords: Lignite ; elemental analysis ; extraction yield ; functional groups ; humic acids ; humic fertilizer ; molecular size ; potassium hydroxide ; temperature
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Enzyme and Microbial Technology 16 (1994), S. 688-695 
    ISSN: 0141-0229
    Keywords: Hybridomas ; dialysis ; mathematical model ; metabolism ; monoclonal antibody ; perfusion
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Enzyme and Microbial Technology 16 (1994), S. 2-9 
    ISSN: 0141-0229
    Keywords: Exoglucanase ; Ruminococcus ; inhibition ; monoclonal antibody
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cytotechnology 19 (1995), S. 55-61 
    ISSN: 1573-0778
    Keywords: codeine ; ELISA ; monoclonal antibody ; opium alkaloid ; qualitative analysis ; thebaine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The ratio of hapten and bovine serum albumin in antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. Monoclonal antibodies against thebaine and codeine were produced by hybridoma fused with the sprenocytes immunized with thebaine- and codeine-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. No cross-reaction of anti-thebaine antibody against morphine was observed. Very small cross-reaction appeared in codeine (0.004%). The cross-reaction of anti-codeine antibody against morphine and thebaine was 2.97 and 5.98%, respectively. The full measuring range of the assay extends from 60 pg mL to 1 ng mL for thebaine and 1 ng mL to 100 ng mL for codeine.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-0778
    Keywords: hybridoma ; monoclonal antibody ; perfusion culture ; mammalian cell culture ; serum-free culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×107 cells/ml, when the specific perfusion rate was set to 2.3 vol day-1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cytotechnology 2 (1989), S. 9-17 
    ISSN: 1573-0778
    Keywords: heterohybridoma ; LDL ; monoclonal antibody ; serum-free medium ; PEG ; swine serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A small amount of swine serum markedly stimulated cell growth for high productivity subclones derived from a mouse human-human heterohybridoma, N12-16.63, secreting an anti-tetanus toxoid human monoclonal antibody in a polyethylene glycol (PEG)-containing serum-free medium, PEG-86-1. A growth promoting substance, SSGF-I, was isolated from the serum by ammonium sulfate fractionation, Cibacron blue F3A-G affinity chromatography, DEAE-agarose ion exchange chromatography, and gel filtrations on Trisacryl GF 2000 and Sephacryl S-300. SSGF-I was characterized as a low density lipoprotein (LDL) of swine serum by its physico-chemical properties. It promoted cell growth synergistically with PEG and its optimum concentration was 1 to 100μg/ml. Human LDL was less active, and human or swine high density lipoprotein (HDL) and very low density lipoprotein (VLDL) were inactive. Based on these results, we propose an improved serum-free medium, PEG-86-3, which contains all the ingredients of PEG-86-1 and 10μg/ml SSGF-I. This medium is useful for not only high productivity heterohybridomas but also for a variety of lymphoid cell lines.
    Type of Medium: Electronic Resource
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