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1

Digitale Medien

Studies on factors influencing methane production from tomato-processing waste

Sarada, R. ; Joseph, R.

Amsterdam : Elsevier

Bioresource Technology 47 (1994), S. 55-57

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ISSN: 0960-8524

Schlagwort(e): Tomato-processing waste ; hydraulic retention time ; loading rate ; methane ; temperature

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/0960-8524(94)90028-0

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2

Digitale Medien

Study of agglomeration characteristics of silica sand-straw ash mixtures using scanning electronic microscopy and energy dispersion X-ray techniques

Ghaly, A.E. ; Ergudenler, A. ; Laufer, E.

Amsterdam : Elsevier

Bioresource Technology 48 (1994), S. 127-134

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ISSN: 0960-8524

Schlagwort(e): Agglomeration ; X-ray analysis ; eutectic ; liquidus ; potassium oxide ; scanning electron microscopy ; silica ; straw ash ; temperature

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/0960-8524(94)90199-6

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3

Digitale Medien

Effects of the extraction temperature on the characteristics of a humic fertilizer obtained from lignite

Garcia, D. ; Cegarra, J. ; Roig, A. ; [weitere]

Amsterdam : Elsevier

Bioresource Technology 47 (1994), S. 103-106

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ISSN: 0960-8524

Schlagwort(e): Lignite ; elemental analysis ; extraction yield ; functional groups ; humic acids ; humic fertilizer ; molecular size ; potassium hydroxide ; temperature

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/0960-8524(94)90106-6

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4

Digitale Medien

Hybridoma growth and monoclonal antibody production in a dialysis perfusion system

Amos, B. ; Al-Rubeai, M. ; Emery, A.N.

Amsterdam : Elsevier

Enzyme and Microbial Technology 16 (1994), S. 688-695

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ISSN: 0141-0229

Schlagwort(e): Hybridomas ; dialysis ; mathematical model ; metabolism ; monoclonal antibody ; perfusion

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/0141-0229(94)90091-4

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5

Digitale Medien

Inhibition of the exo-β-1,4-glucanase from Ruminococcus flavefaciens FD-1 by a specific monoclonal antibody

Doerner, K.C. ; Gardner, R.M. ; Schook, L.B. ; [weitere]

Amsterdam : Elsevier

Enzyme and Microbial Technology 16 (1994), S. 2-9

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ISSN: 0141-0229

Schlagwort(e): Exoglucanase ; Ruminococcus ; inhibition ; monoclonal antibody

Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1016/0141-0229(94)90102-3

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6

Digitale Medien

Production of monoclonal antibodies and ELISA for thebaine and codeine (1995)

Shoyama, Yukihiro ; Fukada, Tomohiro ; Murakami, Hiroki

Springer

Cytotechnology 19 (1995), S. 55-61

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ISSN: 1573-0778

Schlagwort(e): codeine ; ELISA ; monoclonal antibody ; opium alkaloid ; qualitative analysis ; thebaine

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract The ratio of hapten and bovine serum albumin in antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. Monoclonal antibodies against thebaine and codeine were produced by hybridoma fused with the sprenocytes immunized with thebaine- and codeine-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. No cross-reaction of anti-thebaine antibody against morphine was observed. Very small cross-reaction appeared in codeine (0.004%). The cross-reaction of anti-codeine antibody against morphine and thebaine was 2.97 and 5.98%, respectively. The full measuring range of the assay extends from 60 pg mL to 1 ng mL for thebaine and 1 ng mL to 100 ng mL for codeine.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00749755

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7

Digitale Medien

High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium (1989)

Tokashiki, Michiyuki ; Arai, Takami

Springer

Cytotechnology 2 (1989), S. 5-8

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ISSN: 1573-0778

Schlagwort(e): hybridoma ; monoclonal antibody ; perfusion culture ; mammalian cell culture ; serum-free culture

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×107 cells/ml, when the specific perfusion rate was set to 2.3 vol day-1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00365409

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8

Digitale Medien

SSGF-I, a potent growth-promoting substance for mammalian cells from swine serum (1989)

Shintani, Yasushi ; Iwamoto, Keiji ; Kitano, Kazuaki

Springer

Cytotechnology 2 (1989), S. 9-17

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ISSN: 1573-0778

Schlagwort(e): heterohybridoma ; LDL ; monoclonal antibody ; serum-free medium ; PEG ; swine serum

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract A small amount of swine serum markedly stimulated cell growth for high productivity subclones derived from a mouse human-human heterohybridoma, N12-16.63, secreting an anti-tetanus toxoid human monoclonal antibody in a polyethylene glycol (PEG)-containing serum-free medium, PEG-86-1. A growth promoting substance, SSGF-I, was isolated from the serum by ammonium sulfate fractionation, Cibacron blue F3A-G affinity chromatography, DEAE-agarose ion exchange chromatography, and gel filtrations on Trisacryl GF 2000 and Sephacryl S-300. SSGF-I was characterized as a low density lipoprotein (LDL) of swine serum by its physico-chemical properties. It promoted cell growth synergistically with PEG and its optimum concentration was 1 to 100μg/ml. Human LDL was less active, and human or swine high density lipoprotein (HDL) and very low density lipoprotein (VLDL) were inactive. Based on these results, we propose an improved serum-free medium, PEG-86-3, which contains all the ingredients of PEG-86-1 and 10μg/ml SSGF-I. This medium is useful for not only high productivity heterohybridomas but also for a variety of lymphoid cell lines.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00365410

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9

Digitale Medien

A serum-free medium for hybridoma cell culture (1993)

Chen, Zhihong ; Ke, Yongzhong ; Chen, Yinliang

Springer

Cytotechnology 11 (1993), S. 169-174

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ISSN: 1573-0778

Schlagwort(e): cell culture ; hybridoma ; monoclonal antibody ; serum-free medium

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract The effects of several different substances, including insulin, transferrin, ethanolamine, selenite and butyrate on the growth of murine hybridoma 2F7 cells, which secrete monoclonal antibody against small cell lung cancer, were investigated, and a serum-free medium SFMI was formulated. The effects of taurine, spermidine, progesterone and adenine on the cell growth were tested further on the basis of the medium SFMI, and a modified serum-free medium SFM II was established. On the basis of medium SFM II, the substitution tests of ferric citrate for transferrin were carried out, and it was found that transferrin could be replaced. The experiments suggested that the formulated serum-free medium was suitable for 2F7 cell growth and monoclonal antibody secretion, and thus facilitated subsequent purification of monoclonal antibody.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00749866

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10

Digitale Medien

Rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonal antibody (1999)

Putalun, Waraporn ; Tanaka, Hiroyuki ; Shoyama, Yukihiro

Springer

Cytotechnology 31 (1999), S. 153-158

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ISSN: 1573-0778

Schlagwort(e): immunoaffinity column ; MALDI-MS ; monoclonal antibody ; solasodine glycosides

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract Immunoaffinity column using anti-solamargine monoclonal antibody for separation of solasodine glycosides was established. This method was specific for solasodine glycosides which was detected by thin layer chromatography and the western blotting. Total solasodine glycosides have been separated directly from the crude extract of Solanum khasianum fruit by the newly established immunoaffinity column.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1008032524419

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