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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Insectes sociaux 38 (1991), S. 171-188 
    ISSN: 1420-9098
    Keywords: Halictidae ; Lasioglossum ; colony dynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Proximate control of colony dynamics was studied in the primitively eusocial halictine beeLasioglossum (Dialictus) zephyrum using allozyme markers. The results indicate that workers produce on average 15% of the male brood (range=0–50%) in small laboratory colonies made up of unrelated, single-generation, uninseminated females. This proportion is not influenced by colony size, but is influenced by the relative size of the queen. Large queens are more successful in dominating their workers than are small queens, the queen being defined as the female that is the mother of most of the brood produced in the colony. Older and larger females tend to become queens. Thus, while small differences in age (up to 4 days) influence which female becomes a queen, her ability to control her workers is primarily influenced by her relative size. The proportion of reproduction that is co-opted by the queen is negatively correlated with colony reproductivity (the number of males/day/female). Colony reproductivity is also negatively correlated with the standard deviation in size among females.
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  • 2
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    Insectes sociaux 38 (1991), S. 195-204 
    ISSN: 1420-9098
    Keywords: Formicidae ; Tapinoma ; population biology ; Australia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Nests ofTapinoma minutum were collected and mapped from a wet sclerophyll forest in New South Wales during the spring, summer, and fall seasons. Queen number was variable, indicating the population is both facultatively polygynous and polydomous. Electrophoretic data from three polymorphic enzymes revealed that relatedness among workers conformed to the Hamiltonian expectation of 0.75. Colony boundaries were inferred from electrophoretic data synthesized with nest spatial locations. For this species colonies were composed of at most three nests; this simple pattern of polydomy suggests it has a recent origin in this population. The pattern of facultative polygyny may be linked to an apparent high rate of colony orphaning.
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  • 3
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    Insectes sociaux 38 (1991), S. 217-218 
    ISSN: 1420-9098
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 4
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    Insectes sociaux 38 (1991), S. 213-216 
    ISSN: 1420-9098
    Keywords: Ropalidia marginata ; Unmated queens ; Individual selection ; Evolution of sociality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the primitively eusocial tropical waspRopalidia marginata, five out of eleven colonies studied had an unmated female as their queen. In two colonies this was the case despite the presence of another mated individual in the colony. We found no detectable differences between colonies with unmated queens and those with mated queens. We argue that in species such asR. marginata, where intracolony relatedness is expected to be low and where sociality is likely to be maintained because several individuals have opportunities for direct reproduction in the future, individual selection is likely to override “the good of the colony” and lead to such phenomena as that of unmated queens.
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  • 5
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    Insectes sociaux 38 (1991), S. 219-220 
    ISSN: 1420-9098
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 6
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    Insectes sociaux 38 (1991), S. 251-262 
    ISSN: 1420-9098
    Keywords: Isoptera ; Termitidae ; Macrotermitinae ; instar duration ; production ; biomass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Production in mature termite nests consists of a seasonal brood of reproductives and a continuous turnover of steriles. The sterile population of the nest remained fairly constant, with no regular seasonal fluctuations. Growth rates of steriles were estimated by interrupting the input and following the “missing cohort”. Estimated mean values of standing crop biomass and annual production in a mature nest were used to calculate a production-to-biomass ratio. In conjunction with data on the density of nests in the field, the biomass and production per hectare were estimated.
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  • 7
    ISSN: 1420-9098
    Keywords: Honey bee ; Apis mellifera ; queen pheromone ; age effect ; olfactory behavior ; olfactometer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Behavioral responses of differently aged worker beesApis mellifera to a queen pheromonal extract were analysed. The bees were tested individually in a four-armed olfactometer, one arm being scented with the pheromonal extract. This extract was prepared from heads of 14–17-day-old unmated queens. Among the components of the blend, 470 μg 9-keto-2-(E)-decenoic acid, 200 μg 9-hydroxy-2-(E)-decenoic acid and 5 μgp-hydroxybenzoic acid methyl ester per queen equivalent were dosed. An age dependency in the worker bees' olfactory response to the components of the queen extract was shown, the strongest response occurring below the age of 5 days.
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  • 8
    ISSN: 1420-9098
    Keywords: Ontogeny ; Formicidae ; learning ; queen attractant cues ; queen recognition ; Cataglyphis cursor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The behaviour ofCataglyphis cursor workers towards queens at 15 days, one month or two months after worker emergence was tested. Workers reared entirely with their own maternal queen were tested with this queen or with an unfamiliar alien queen. Workers transferred within 48 h of emerging to a new definitive nest with an alien queen were tested with this queen or with the original maternal queen. The degree of attraction to each of these queens and the workers' behavioural repertoire were measured and analysed. The results showed the following: 1) The attractiveness of queens and the workers' queen recognition behaviour were linked. 2) Although unfamiliar alien queens hardly attract workers, familiar alien queens were as attractive as maternal queens, and induced the same strongly marked and unique worker response, indicating that workers learn queen attractant cues in the days immediately after emergence. 3) Agonistic reactions were observed, but workers continued to be attracted to their maternal queen even after developing an attraction response to an alien queen with which they had been reared. These results agree with the proposal that queens produce two kinds of pheromones, those that attract workers and those that mediate recognition of queens by workers. These results show the ability of workers to discriminate between queens. Workers are attracted to any queen, but recognize as nestmates only maternal or alien queens with which they have been maintained. 4) The differential in worker attraction and recognition from 15 days to 2 months and its modifications by post-imaginal experience illustrate worker behavioural ontogeny, which is a basis of social discrimination.
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  • 9
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    Insectes sociaux 38 (1991), S. 307-316 
    ISSN: 1420-9098
    Keywords: Dolichoderus ; silk production ; worker ; nest building ; rain forest ; Malaysia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a montane Malayan rain forest, at an elevation of about 900 m above sea level, we found an undetermined and possibly undescribedDolichoderus species of thethoracicus group, living in colonies consisting of 50–100 silken pavilions on the undersides of leaves of different species of trees. Inside these pavilions, the ants kept scale insects, which we never found outside the nests on the colony tree. The stock of symbionts was actively regulated; supernumerary scale insects were thrown to the ground by the workers. New pavilions were colonized with scale insects. Our observations and behavioural experiments revealed that the silken material is produced by neither the brood nor the scale insects, but by the worker ants. This is the first proof of weaver ants outside the subfamily Camponotinae.
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  • 10
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    Insectes sociaux 38 (1991), S. 333-334 
    ISSN: 1420-9098
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 11
    ISSN: 1420-9098
    Keywords: Thaumatomyrmex ; taxonomy ; comparative morphology ; predation ; Polyxenidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe, for the first time, the predatory behaviour ofThaumatomyrmex ants on millipedes of the family Polyxenidae, based on field observations ofT. atrox and a field and laboratory study ofT. contumax. The capture of the prey and the removal process of its body-covering setae by the ants before they eat the millipede are described. This specialized behaviour in at least two species of the genus, belonging to two distinct groups of species, indicates a general trend inThaumatomyrmex. We coupled this study with a comparative morphological analysis of the mouthparts and digestive tube of these and otherThaumatomyrmex species. Also, we report the first case of sympatry in the genus, which suggests thatThaumatomyrmex includes several species, and not only one highly variable taxon, as hypothetized earlier.
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  • 12
    ISSN: 1420-9098
    Keywords: Soldier ; social behaviour ; aphid ; Pseudoregma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Life history and behavioural characteristics of the bamboo aphid,Pseudoregma bambucicola (Takahashi), which has sterile soldiers, were studied in the laboratory. The stadium of normal (fertile) first instar larvae was two times longer than that of second instar larvae, and the stadium of soldier-type (sterile) first instar larvae was much longer (max. 116 days) than the stadium of normal first instar, suggesting that soldiers are able to take nutrition from bamboo. Stimulation of larvae with breath, vibration of bamboo shoots or disturbing the larvae with the tip of a fine brush induced significantly more defensive acts by soldiers than those by normal larvae — the latter usually fled. Soldiers did not attack non-kin conspecific intruders or even aphids of different species, suggesting that, in this species, kin-recognition ability is low.
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  • 13
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    Insectes sociaux 38 (1991), S. 331-332 
    ISSN: 1420-9098
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 14
    ISSN: 1420-9098
    Keywords: Nuptial flights ; sexual behaviour ; polygyny ; Leptothoracini
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe in detail a very large nuptial flight of the antLeptothorax acervorum at an open hilltop site in Britain. The mating behaviour of these ants involved not only a large mating swarm but also sexual/calling behaviour by the females. The females left the flight to land on vertical objects, where they took up a characteristic calling posture, in which females of closely related species are known to release pheromones that are sexually attractive to males. ThatLeptothorax acervorum has a complex mating behaviour involving both large nuptial flights and sexual calling has important consequences for the interpretation of the evolution of polygyny in this species and social parasitism in its close relatives.
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  • 15
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    Insectes sociaux 38 (1991), S. 345-349 
    ISSN: 1420-9098
    Keywords: Chemical communication ; exploration ; Lasius pallitarsis ; novelty ; recruitment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Lasius pallitarsis ants were placed in situations where they encountered unfamiliar areas and had to choose between one of two directions for further exploration. Workers advancing onto new ground apparently leave behind some chemical signature to which later ants orient. This orientation occurred under two types of experimental conditions. First, ants show a significant tendency to follow each other as they advance out from their colony into unexplored areas. The same ants transferred into an entirely novel situation, in which there is no obvious “homeward” direction, show similar behavior. When ants are coming from familiar ground, following tends to increase as the number of ants passing the decision point increases. However, following decreases when ants are on entirely novel ground. The results are discussed in relation to models describing foraging responses.
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  • 16
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    Insectes sociaux 38 (1991), S. 351-363 
    ISSN: 1420-9098
    Keywords: Central Spain ; cereal croplands ; density ; distribution ; Messor ; nests ; site selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Patterns of abundance and site selection of granivorous ant nests were investigated in extensive cereal croplands of Central Spain. Nest densities and distributions were measured in two consecutive summers (1988 and 1989), together with habitat physiognomy and seed availability. Nest site selection patterns were analysed at two spatial scales (landscape and microhabitat) with respect to habitat physiognomy. Results indicate a very constant and predictable pattern of both nest abundance and nest site selection. Granivorous ant nests were most abundant in shrublands, and shrubby microsites were selected for nest placement. Croplands, and microsites with high covers of bare ground and litter, were avoided. These patterns were consistent between years despite a 1.7-fold increase in shrubland nest densities, that was attributed to the exceptionally dry winter between nest censuses. I suggest that winter survivorship of ant nests in the unploughed landscape units, and periodic ploughing in croplands, may be the main factors constraining granivorous ant densities in the landscape studied.
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  • 17
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    Insectes sociaux 38 (1991), S. 423-437 
    ISSN: 1420-9098
    Keywords: Stenogastrinae ; Anischnogaster ; social behaviour ; colony biology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Level of social organization, adult behaviour, size and development of the brood in three species ofAnischnogaster are described. The normal colony size in all the three species was one female per nest, and colony size never exceeded two females per nest. The social interactions, which were only observed in one species, did not include any marked dominance behaviour, but there was clear caste differentiation, with the older female guarding the nest while the younger female foraged for food. InAnischnogaster sp. A only some eggs and larvae have abdominal secretion, while no secretion at all was found on the eggs and larvae ofA. laticeps. In spite of this, the Dufour's gland was found to be well developed. The significance of this is discussed. Females ofA. laticeps were found to fall into two groups distinguished by the length of the sting. The larvae seem quite similar to those of other Stenogastrinae and have, apparently, only four instars.
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  • 18
    ISSN: 1420-9098
    Keywords: Tropical soils ; porosity ; Isoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary Insights on the porous properties and evolving sizes of sample pores have been derived by means of physical measures carried out on several soil samples of various sizes placed under a hydric strain of 0.1 MPa. Under the very influence ofT. macrothorax (humivorous termite), surface horizons are the first to be altered. Within one metre's radius around their nest, a significant increase of porosity is noticeable, reaching an exceptionally high degree at the bottom of the nest. The volume of pores 〉 1.5 μm is held to be considerably amplified as compared with control soils; however, the interference 〈 1.5 μm pores. The masticative and mechanical power of termites on the soil, together with the organic matter bulk increase turn out to play a paramount role in the stability ofT. macrothorax constructions. On the contrary, horizons in the vicinity ofM. mulleri's nests (fungus growing termite) undergo a diminutive porosity with the total disappearance of pores 〉 1.5 μm. A sharp decrease or organic matter rates has been recorded. Alternatively, as far asM. mulleri are concerned, physical and physico-chemical data do emphasize that the soil structural stability is not to be correctly ensured by the cohesive properties which are normally to be taken into account with this type of material.
    Notes: Résumé A partir de mesures physiques réalisées sur des échantillons de sols de différentes tailles portés a une contrainte hydrique de 0,1 MPa on obtient des informations sur la porosite et l'évolution de la taille des pores des échantillons. L'action deT. macrothorax (termite humivore) sur le sol affecte en premier lieu les horizons de surface. On note, à 1 mètre autour du nid, une forte augmentation de la porosité qui devient exceptionnellement élevée au pied de la termitière. Le volume des pores de 〉 à 1,5 μm est considérablement amplifié par rapport au sol témoin mais la contribution des pores de 〈 à 1,5 μm diminue. L'action mécanique de mastication du sol par les termites conjuguée à l'augmentation de matière organique apparait jouer un rôle décisif dans la stabilité des contructions deT. macrothorax. Les horizons avoisinants le nid deM. mülleri (termite champignonniste) ainsi que la muraille du nid enregistrent une diminution de la porosité avec disparition complète des pores 〉 1,5 μm. On constate dans ce cas une baisse du taux de matière organique dans les échantillons. Dans le cas deM. mülleri les données physiques et physico-chimiques montrent que la stabilité structurale du sol ne peut être vraisemblablement pas assurée par les forces de cohésion envisagées habituellement dans ce genre de matériau.
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  • 19
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    Pure and applied geophysics 136 (1991), S. 87-101 
    ISSN: 1420-9136
    Keywords: Curie point ; aeromagnetic ; heat flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The objective of this study is to understand the nature and extent of the regional geothermal system at depth beneath the area of Greece by constructing the Curie isotherms. Spectral analysis of aeromagnetic data in conjunction with heat flow information revealed an almost inverse linear relation between heat flow and Curie depths and was used to construct the Curie isotherms from the existing heat flow data. The results showed that Curie depths in the area range from about 20 km in western Greece, up to 1 km beneath the Hellenic volcanic arc. These results are consistent with the existing geothermal and geotectonic regime in the area.
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  • 20
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    Pure and applied geophysics 136 (1991), S. 143-154 
    ISSN: 1420-9136
    Keywords: Earthquake statistics ; earthquake hazard ; Vrancea seismogenic region
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract A maximum likelihood method is used to estimate the earthquake hazard parameters maximum magnitudeM max, annual activity rate λ, and theb value of the Gutenberg-Richter equation in the Vrancea (Romania) region. The applied procedure permits the use of mixed catalogs with incomplete historical as well as complete instrumental parts, the consideration of variable detection thresholds, and the incorporation of earthquake magnitude uncertainty. Our imput data, comprises 105 historical earthquakes which occurred between 984 and 1934, and a complete data file containing 1067 earthquakes which occurred during the period 1935–30 August, 1986. The complete part was divided into four subcatalogs according to different thresholds of completeness. Only subcrustal events were considered, and dependent events were removed. The obtained $$\hat b$$ value (=0.65) is at the lower range of the previously reported results, but it appears concurrent with conceptual and observational facts. The same concerns inferred value of $$\hat M$$ max = 7.8 and activity rate $$\hat \lambda $$ 4.0 = 5.34.
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  • 21
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    Pure and applied geophysics 137 (1991), S. 367-390 
    ISSN: 1420-9136
    Keywords: Strain localisation ; bifurcation ; finite-element analysis ; micro-polar continua ; strain softening ; non-associated plasticity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Methods are reviewed for analysing highly localised failure and bifurcation modes in discretised mechanical systems as typically arise in numerical simulations of failure in soils, rocks, metals and concrete. By the example of a plane-strain biaxial test it is shown that strain softening and lack of normality in elasto-plastic constitutive equations and the ensuing loss of ellipticity of the governing field equations cause a pathological mesh dependence of numerical solutions for such problems, thus rendering the results effectively meaningless. The need for introduction of higher-order continuum models is emphasised to remedy this shortcoming of the conventional approach. For one such a continuum model, namely the unconstrained Cosserat continuum, it is demonstrated that meaningful and convergent solutions (in the sense that a finite width of the localisation zone is computed upon mesh refinement) can be obtained.
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  • 22
    ISSN: 1420-9136
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  • 23
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    Pure and applied geophysics 137 (1991), S. 95-112 
    ISSN: 1420-9136
    Keywords: Acoustic emission ; friction ; holography ; irregular surface ; precursor ; rock physics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract In this paper we report results obtained from various friction experiments under direct and oblique shear loading conditions. We used four rock types of varying brittleness (quartzite, anhydrite, limestone, pyrophyllite) with different surface roughness. The observations concentrate on the time span several milliseconds before dynamic failure occurs. During this period a premonitory, unstable phase of slip (slip 2) occurs. This differs importantly from a premonitory, stable process (slip 1) with durations of hundreds of seconds. On smooth surfaces slip 2 is usually observed with ductile rocks and less reliably with brittle rocks. Slip 2 is mostly accompanied by acoustic emissions, which increase in rate of occurrence and in magnitude until the stick-slip event. Foreshocks are observed during approximately 50% of the slip 2 events on rough surfaces. Foreshocks far exceed the “acoustic noise level”, which is also prevalent before stick-slip events on rough surfaces. In the direct shear experiment, where two faults are being loaded simultaneously, in about 20% of the cases precursory slip 2 was observed on the opposite side on which the final stick-slip event occurred.
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  • 24
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    Pure and applied geophysics 137 (1991), S. 175-199 
    ISSN: 1420-9136
    Keywords: Induced seismicity ; source parameters ; stress drops ; rock mechanics ; strength
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract This study attempts to determine the relation between source parameters and mechanical properties of the rock matrix in which the microseismic events occur. For this purpose, accurate geological, mechanical and seismological data were acquired on a gas field experiencing induced seismicity due to its reservoir pressure drop. More than 30 deep boreholes (depth greater than 4 km) are concentrated in a 10×10×5 km volume, providing core samples for both geological and mechanical assessment. In this study, we focus on induced seismic events recorded by the local seismic network, over a three-year-long period. Characteristics of the seismic sources were obtained using spectral analysis and a dynamic model of failure. Results point out correlation between physical parameters of the seismic sources and the geomechanical properties of the rocks involved. Maximal static stress drops are found to be associated with the mechanical strength of the geological strata where the rupture occurs. The fracture size, using a circular model of failure, is also found to be dependent on the geomechanical setting. It is found that the size of the seismic fractures is dependent on the layer thickness and the prefracturation of the medium, both factors influencing the extension of preexisting discontinuities. The parameters of the seismic sources also show important changes when the gas reservoir is reached. The reservoir unit experienced a 45 MPa pore fluid pressure drop over a period of 20 years.
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  • 25
    ISSN: 1420-9136
    Keywords: Moment tensor inversion ; North Aegean ; Greece
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Average source parameters for three large North Aegean events are obtained from body wave inversion for the moment tensor. The parameters for the events are as follows: The events exhibit dextral strike-slip faulting with theT axis striking NS and nearly horizontal, implying extension in this direction. The focal mechanisms obtained are in agreement with the seismotectonic regime of the North Aegean. It is known that the region is tectonically controlled by the existence of the strike-slip Anatolian fault and its westward continuation in the Aegean, as well as the NS extension the whole Aegean area undergoes. The components of the moment tensor show that the region is dominated by compression in the EW direction which is encompassed by extension in the NS direction. All the events were found to be shallow (≤10 km) with a source time function of approximately 8 s duration and small stress drop values. The teleseismic long period verticalP-waves exhibited distortions, that could be attributed to lateral inhomogeneities in the source structure or more probably to a nonflat water-crust interface.
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  • 26
    ISSN: 1420-9136
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  • 27
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    Pure and applied geophysics 137 (1991), S. 409-419 
    ISSN: 1420-9136
    Keywords: Fault gouge ; dilatancy ; shear localization ; friction ; Coulomb failure ; mechanical healing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Theoretical constraints on the stress-dilation relation for a deforming Coulomb material requirev≤θ ifC=0 andv ≤ sin-1(τ m /σ m ) always, wherev is the dilation angle, θ is the friction angle,C is cohesion, τ m is the maximum shear stress, and σ m is the mean effective stress. Recent laboratory measurements of friction and dilatancy of simulated fault gouge show that small amplitude shear-load cycling causes compaction and consolidation. Comparison of the data with theory indicates that such load cycling produces: (1) increased coefficient of friction (or friction angle), (2) increased cohesion, and (3) increased dilatancy rate (or dilation angle). Under certain conditions of load cycling without significant plastic shear strain accumulation ( p 〈0.005) we find thatv exceeds both θ and, in contrast to theory, sin-1(τ m /σ m ). This result is interpreted in terms of enhanced cohesion and overconsolidation, which lead to residual stresses within the gouge. An analogy is drawn between these special loading conditions and those extant on natural faults. In particular, our results imply that jostling and minor stress variations associated with microearthquakes may produce strengthening of fault gouge and changes in the fault zone's stress-dilatancy relation. Hence, compaction associated with microseismicity may lead to subsequent dilation of fault gouge, even for faults with large displacement rates and large net offsets (e.g., San Andreas). In regions where such dilation persists over sufficient displacements (on the order of the critical slip distance for seismic faulting) it may tend to inhibit unstable slip.
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  • 28
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    Pure and applied geophysics 136 (1991), S. 59-86 
    ISSN: 1420-9136
    Keywords: Cataclasis ; particle size reduction ; fractal ; fracture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The particle size distribution (P.S.D.) of fragmented geological materials is affected by the fragmentation process, initial size distribution, number of fracturing events, energy input, strain, and confining pressure. A summary of literature shows that the fractal dimension (D) of the P.S.D. is increased by the number of fracturing events, energy input, strain, and confining pressure. Cenozoic cataclasis of granite, granodiorites, gneisses and arkose seen in cores from the Cajon Pass drillhole, southern California, produced P.S.D.s with values ofD that varied from 1.88 to 3.08. Each rock type has a characteristic and more limited range ofD. Areas of dilatant texture and modeI fracture-fillings have low average values (2.32 and 2.37) compared to an average value of 2.67 in shear fracture-fillingsD has a good inverse correlation with average particle size. Data from fault rocks in the San Gabriel fault zone, southern California (Anderson et al., 1983) have been reanalyzed to show that values ofD are higher (2.10–5.52) and average particle size is lower than the Cajon Pass samples, but the ranges of values overlap, and the inverse correlation betweenD and average particle size is extended. Microstructural observations combined with these results suggest that three processes contributed to particle size reduction during cataclasis. The first process of feldspar alteration, which leads to low values ofD, has not been previously recognized. The second process is probably constrained comminution (Sammis et al., 1987), since the averageD in shear fracture-fillings is close to the value of 2.58 predicted by this theory. A further stage of particle size reduction is demonstrated by an increase ofD with cataclasis. This third process is selective fracture of larger particles, which may also operate during localization and the cataclastic flow-to-faulting transition as observed in experiments. A transition from constrained comminution to selective fracture of large particles, and increasingD values with cataclastic evolution and grain size reduction, may be general features of experimental and natural cataclasis.
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    Pure and applied geophysics 136 (1991), S. 173-189 
    ISSN: 1420-9136
    Keywords: Ring complexes ; spectral analysis ; magnetic source depth estimation ; Hilbert transformation ; hydrothermal alteration ; Pan-African shear zones
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    Topics: Geosciences , Physics
    Notes: Abstract The results of a magnetic study of part of the Younger Granite Province of Nigeria are presented here. Spectral analysis of the magnetic anomalies over the area has been carried out in an effort to estimate the depth to magnetic sources. Average magnetic source depth of 286 m has been obtained for the area and this is thought to be related to the depth to the top of the main anomalous structures in the area. Observed magnetic anomaly profiels taken across the area have been interpreted in terms of arbitrarily shaped bodies—whose existence was confirmed by 3-D Hilbert transformation-using nonlinear optimization techniques. The modelled bodies occur at depths of 200–760 m and have magnetizations of 0.29–0.47 A/m. The results from the analysis of the magnetic field are discussed in relation to the results from previous gravity studies over the area and the mode of emplacement of the Younger Granites.
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    Pure and applied geophysics 135 (1991), S. 53-59 
    ISSN: 1420-9136
    Keywords: Surface wave ; lithospheric structure ; Indian peninsula
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    Topics: Geosciences , Physics
    Notes: Abstract Group velocities of fundamental mode Rayleigh and Love waves have been obtained across the central highlands in the northwestern part of the Indian peninsula and across the plateau region of the peninsula. Such data across the latter region were also obtained earlier and revealed model lithosphere IP11 for this region. Data of the two regions are similar and show that the lithospheric model IP11 continues from the plateau region to the central highlands. The model IP11 has been verified by comparing observed Love wave forms with synthetics ones.
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  • 31
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    Pure and applied geophysics 135 (1991), S. 91-106 
    ISSN: 1420-9136
    Keywords: Seismotectonics ; attenuation ; coda waves
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    Topics: Geosciences , Physics
    Notes: Abstract The seismic energy attenuation in the frequency range of 1–18 Hz was studied in the two tectonically active zones of Irno Valley (Southern Italy) and Granada Basin (South-East Spain). Data were recorded by short period vertical components seismographs for low-magnitude local earthquakes. The method of coda waves, assuming singleS toS scattering approximation, was used to calculate the quality factorQ from the two data set. Results show a quality factor increasing with frequency, following the empirical lawQ=Q o f n .Q o andn are lower for the Irno Valley than for Granada. This result is interpreted in terms of different scattering environments present in the two investigated areas.
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  • 32
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    Pure and applied geophysics 136 (1991), S. 421-432 
    ISSN: 1420-9136
    Keywords: Crustal deformation ; North Aegean trough ; North Anatolia
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    Topics: Geosciences , Physics
    Notes: Abstract The rates and configuration of seismic deformation in the North Aegean trough-North Anatolian fault are determined from the moment tensor mechanisms of the earthquakes that occurred within this region. The analysis is based onKostrov's (1974) formulation. The fault plane solutions of the earthquakes of the period 1913–1983 withM s ≥6.0 are used. The focal mechanism of some of the past events (before 1960) is assumed, based on the present knowledge of the seismotectonics as well as on the macroseismic records of the area studied. The analysis showed that the deformation of the northern Aegean is dominated by EW contraction (at a rate of about 15 mm/yr) which is relieved by NS extension (at a rate of about 9 mm/yr). It was also shown that the northern part of North Anatolia (north of 39.7°N parallel) undergoes contraction in the EW direction (at a rate of about 9 mm/yr) and NS extension as the dominant mode of deformation (at a rate of about 5 mm/yr). It may be stated therefore, that the pattern of deformation of the northern Aegean and the northern part of North Anatolian fault is controlled by the NS extension the Aegean is undergoing as a whole, and the dextral strike-slip motion of the North Anatolian fault. The southern part of North Anatolia is undergoing crustal thinning at a rate of 2.3 mm/yr, NS extension (at a rate of 5 mm/yr) as well as EW extension (at a rate of 4 mm/yr), which are consistent with the occurrence of major normal faulting and justify the separation of North Anatolia into two separate subareas.
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  • 33
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    Pure and applied geophysics 136 (1991), S. 459-477 
    ISSN: 1420-9136
    Keywords: Subduction zone ; New Britain earthquakes ; mature seismic gap ; earthquake prediction
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    Topics: Geosciences , Physics
    Notes: Abstract To better understand the mechanics of subduction and the process of breaking a mature seismic gap, we study seismic activity along the western New Britain subduction segment (147°E–151°E, 4°S–8°S) through earthquakes withm b ≥5.0 in the outer-rise, the upper area of subducting slab and at intermediate depths to 250 km, from January 1964 to December 1990. The segment last broke fully in large earthquakes of December, 28, 1945 (M s =7.9) and May 6, 1947 (M s =7.7.), and its higher seismic potential has been recognized byMcCann et al., (1979). Recently the segment broke partially in two smaller events of February, 8, 1987 (M s =7.4) and October 16, 1987 (M s =7.4), leaving still unbroken areas. We observe from focal mechanisms that the outer-rise along the whole segment was under pronounced compression from the late 60's to at least October 1987 (with exception of the tensional earthquake of December 11, 1985), signifying the mature stage of the earthquake cycle. Simultaneously the slab at intermediate depths below 40 km was under tension before the earthquake of October 16, 1987. That event, with a smooth rupture lasting 32 sec, rupture velocity of 2.0 km/sec, extent of approximately 70 km and moment of 1.2×1027 dyne-cm, did not change significantly the compressive state of stress in the outer-rise of that segment. The earthquake did not fill the gap completely and this segment is still capable of rupturing either in an earthquake which would fill the gap between the 1987 and 1971 events, or in a larger magnitude event (M s =7.7–7.9), comparable to earthquakes observed in that segment in 1906, 1945 and 1947.
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    Pure and applied geophysics 136 (1991), S. 127-141 
    ISSN: 1420-9136
    Keywords: Site effects ; coda waves ; local seismicity
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    Topics: Geosciences , Physics
    Notes: Abstract The method of coda waves was applied to two different sets of data for the evaluation of the relative site response. The first set of data consists of low magnitude earthquakes with closely spaced locations, recorded at a small aperture array of velocimeters located in the Abruzzo region, central Italy. The second set of data is composed of events with epicentral distance ranging from 20 to 300 km, recorded at a seismological network with an aperture of about 100 km located in the Puglia region, southern Italy. Results show that the coda wave method furnishes stable estimates of the site effect. An amplification, relative to an arbitrary site, of a factor of about 2 occurs in the 1.7–6 Hz frequency band for two stations of the Abruzzo network, while an amplification factor of about 0.5 occurs in the whole frequency band (1–24 Hz) for one of the stations of the Puglia network. This station is located in an area which is correlated with a low macroseismic intensity anomaly.
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    Pure and applied geophysics 136 (1991), S. 191-200 
    ISSN: 1420-9136
    Keywords: Thermal conductivity ; temperature dependence
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    Topics: Geosciences , Physics
    Notes: Abstract The variability of sedimentary thermal conductivities with increasing temperature are explored for their impact on estimates of present-day heat flux and subsurface temperature gradient. For sand thicknesses less than about 10–20 km, or shale thicknesses less than about 40–80 km, the subsurface temperature is closely linearly proportional to the thermal resistance integral obtained in the absence of the temperature dependence of thermal conductivity. Estimates of heat flux should be increased (decreased) by about 5% for sands and decreased by about 1% for shales. For salt, because of the much shorter temperature range over which its thermal conductivity decreases, effects produced by the temperature dependence are more noticeable: heat flux should be increased by around 13%, salt thicknesses in excess of 5 km will yield major (around 30–100°C) changes in their temperature regimes solely as a consequence of the temperature-dependent thermal conductivity, and the linear increase of temperature with increasing thermal resistance is not an adequate approximation but has to be replaced with a more exact exponential increase. The impact of the variations, particularly in the case of salt, for geologic processes is briefly considered.
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  • 36
    ISSN: 1432-041X
    Keywords: Mesoderm induction ; Activin ; Vegetalizing factor ; Xenopus ; Follistatin ; FSH
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    Notes: Summary The induction of mesoderm is an important process in early amphibian development. In recent studies, activin has become an effective candidate for a natural mesoderm-inducing factor. In the present study, we show that follistatin, an activin-binding protein purified from porcine ovary, inhibits the mesoderm-inducing activity of recombinant human activin A (rh activin A), which is identical to the erythroid differentiation factor (EDF). The quantity of follistatin required for effective suppression of activin was more than three-fold that of activin (w:w). Follistatin also inhibited the mesoderm-inducing activity of the vegetalizing factor purified from chick embryos, suggesting that the vegetalizing factor is closely related to activin.
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  • 37
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    Keywords: Concanavalin A ; Molluscan development ; Cleavage arrest ; Egg cortex ; Egg polarity
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    Topics: Biology
    Notes: Summary The effects of the lectin concanavalin A (Con A) on cleavage were studied in early embryos of the gastropodNassarius reticulatus. Progression of the first cleavage furrow is inhibited by incubating eggs before the first cleavage with 0.3–20 μg/ml Con A. Treatment with 1.0–20 μg/ml Con A during first cleavage causes regression of the cleavage furrow. Treatment with low concentrations (0.3–1.0 μg/ml) during the same period does not affect first cleavage. However, when further development of such eggs is followed, one finds that second cleavage is inhibited typically in only one of the two blastomeres of the 2-cell stage, i.e. the CD-blastomere. As a result, a 3-cell embryo is formed. At third cleavage of such embryos, the CD-blastomere forms either one double-sized micromere (1cd-micromere) or two normal-sized micromeres (1c and 1d) simultaneously. Sometimes micromere formation in the CD-blastomere is inhibited. Con A binding does not affect karyokinesis, nor does it affect the division asynchronies typical for normal development. On the basis of these and other results it is argued that binding of Con A to sites located at the vegetal pole of the egg is responsible for the cell lineage-specific inhibition of cleavage by Con A. This effect is most probably mediated by changes in the organization of the egg cortex.
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  • 38
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    Development genes and evolution 200 (1991), S. 27-37 
    ISSN: 1432-041X
    Keywords: Crustacean ; Embryos ; Development ; Staging
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    Topics: Biology
    Notes: Summary Development of the crayfish embryo is described in sequential stages separated by intervals that represent 5% of the total time taken from fertilization to hatching, which is 40 days at 19° C. Early cell division, aggregation of blastoderm cells into the ventral plate, gastrulation and formation of the embryo can be clearly observed through the transparent chorion and each stage characterised using morphological criteria. At hatching the egg chorion splits open but the hatchling (postembryo stage 1) remains attached to the inside of the egg by membranes extending from its tail. 7 to 8 days later the hatchling moults to produce the 2nd post-embryonic stage. Free from the egg, it still clings to the mother. 14 days later the 2nd postembryonic stage moults to produce the immature adult.
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  • 39
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    Development genes and evolution 200 (1991), S. 38-44 
    ISSN: 1432-041X
    Keywords: Chick embryo ; Glycoprotein ; Lens ; Ectoderm
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    Topics: Biology
    Notes: Summary Lens induction is a classic example of the tissue interactions that lead to cell specialization during early vertebrate development. Previous studies have shown that a large region of head ectoderm, but not trunk ectoderm, of 36 h (stage 10) chicken embryos retains the potential to form lenses and synthesize the protein δ-crystallin under some conditions. We have used polyacrylamide gel electrophoresis and fluorography to examine protein and glycoprotein synthesis in presumptive lens ectoderm and presumptive dorsal (trunk) epidermis to look for differentiation markers for these two regions prior to the appearance of δ-crystallin at 50 h. Although nearly all of the proteins incorporating3H-leucine were shared by presumptive lens ectoderm and trunk ectoderm, these two regions showed more dramatic differences in the incorporation of3H-sugars into glycoproteins. when non-lens head ectoderm that has a capacity for lens formation in vitro was labeled, a hybrid pattern of glycoprotein synthesis was discovered: glycoproteins found in either presumptive lens ectoderm or trunk ectoderm were oftentimes also found in other head ectoderm. Therefore, molecular markers have been identified for three regions of ectoderm committed to different fates (lens and skin), well before features of terminal differentiation begin to appear in the lens.
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    Development genes and evolution 200 (1991), S. 45-50 
    ISSN: 1432-041X
    Keywords: Cnidaria ; Scyphozoa ; Aurelia ; Budding ; Strobilation
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    Topics: Biology
    Notes: Summary Strobilation and polypoid budding occur at different locations in the scyphistoma (polyp). Initiation and completion of both forms of budding are inhibited by hydroxyurea (HU), which blocks 95% of DNA synthesis within 12 h. Gradients of thymidine incorporation into both cell layers of the body column precede and accompany strobilation, and an epidermal gradient precedes polypoid budding. In both, the highest labelling index is in the zone in which initiation will occur. Polypoid buds show high variation in labelling index, which is therefore not significantly different from body column labelling. Initiation and some elongation of polyp buds occurs in a small percentage of animals in HU, indicating that cell recruitment is important for these processes. Strobilation appears to be more highly dependent on localized nucleic acid synthesis than polypoid budding.
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    Development genes and evolution 200 (1991), S. 58-60 
    ISSN: 1432-041X
    Keywords: Amphibian ; Gastrulation ; Osmotic stress ; Developmental genetics ; Triturus cristatus
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    Topics: Biology
    Notes: Summary A recent description of genetically abnormal embryos of European crested newts was based on their development in Rugh's “urodele growing medium” (40 mM NaCl). The salinity tolerance of these embryos is tested here, showing that gastrulation is increasingly retarded at salinities above 12 mM, often incomplete above 70 mM and abortive at 120 mM NaCl. The results confirm the previous diagnosis of fat-tailed and slimtailed types of arrested tailbud embryos and show that each type occurs at a frequency of 25%, as expected for the balanced lethal system in this species. The defective gastrulation claimed to be characteristic of the fattailed phenotype, however, was caused by culture in a hypertonic medium.
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    Development genes and evolution 200 (1991), S. 61-63 
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  • 43
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    Development genes and evolution 200 (1991), S. 51-57 
    ISSN: 1432-041X
    Keywords: Glycolysis ; embryos ; Energetics ; embryos ; Metabolism ; embryos ; Xenopus embroys ; metabolism
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    Topics: Biology
    Notes: Summary The major carbon sources inXenopus oocytes and cleavage-stage embryos appear to be amino acids, which are oxidized to form pyruvate (to support the Krebs cycle) and phosphoenolpyruvate (for anabolic processes). Metabolism of various metabolites in vitro into aspartate or glutamate, and then partially into phosphoenolpyruvate, requires the presence of mitochondria, suggesting that metabolism in vivo utilizes mitochondrial enzymes. The rate limiting step in metabolism in the stage VI oocyte appears to be uptake and/or metabolism of compounds by the mitochondria; the rate of metabolism increases during maturation. During early cleavage no qualitative differences in metabolism were observed either as a function of development, or spatially along the animal/vegetal or prospective dorsal/ventral axes.
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  • 44
    ISSN: 1432-041X
    Keywords: Achaete-scute gene complex ; Cell-cell interactions ; Cell differentiation
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    Notes: Summary Cell-cell interactions play a fundamental role in the differentiation of nervous elements in constant patterns, both during embryogenesis and imaginal development. In this paper we analyse the role of genes of theachaete-scute andEnhancer of split complexes, plus the genesextramacrochaetae, Notch, Delta, andHairless in the patterning of sensory elements in the mesonotum ofDrosophila. The phenotypes of different alleles of these genes, including lethals in genetic mosaics, reveal their participation in two processes, the singling out from epidermal cells of sensory organ mother cells and their subsequent differentiation. Studies of allelic combinations of different genes lead to a model of the genetic interactions involved in the processes of pattern formation. In this model, theachaete-scute complex plays a central role, determining sensory organ mother cells and preventing neighbouring cells from following the same developmental pathway.
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    Development genes and evolution 200 (1991), S. 77-85 
    ISSN: 1432-041X
    Keywords: Lysosomes ; Ultrastructure ; Chloroquine ; Blastocyst ; Mouse
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    Topics: Biology
    Notes: Summary Mouse morulae are known to undergo cavitation as soon as some external cells have entered the sixth cell cycle (Garbutt et al. 1987). Since the early cytological features of cavitation are still unclear, we undertook a careful ultrastructural analysis of late morulae-nascent blastocysts. In addition, since maturation of lysosomes might be involved in the first step of cavity formation, we focused our attention on these organelles by means of the cytochemical localization of trimetaphosphatase activity and by the study of the effects of chloroquine on precavitation embryos. Our results suggest that cavitation starts in a few external cells (presumably competent cells entering the sixth cell cycle), by the chloroquine-sensitive formation of degradative autophagic vacuoles engulfing lipid droplets and vacuoles containing osmiophilic material. These complex structures enlarge (as a result of lipid metabolism?) and so transform into intrablastomeric cavities which, by means of a membrane fusion process, very rapidly become extracellular cavities that coalesce. The abembryonic pole of the blastocyst is determined in this way. Moreover, we suggest that the juxtacoelic cytoplasmic processes covering the inner cell mass (ICM) cells, which are known to restrict the expression of their totipotency during early cavitation (Fleming et al. 1984), are the latest remnants of the walls of the growing intrablastomeric cavities.
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  • 46
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    Development genes and evolution 200 (1991), S. 86-94 
    ISSN: 1432-041X
    Keywords: Yolk sac ; Erythrocyte ; Terminal differentiation ; Haemoglobin ; Hamster
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    Topics: Biology
    Notes: Summary The developmental fate of Syrian hamster yolk-sac (primitive) erythroid cells was examined in vitro. Highly purified yolk-sac erythroid cells at the polychromatophilic stage, obtained from the peripheral blood of embryos at day 10 of gestation, showed morphological and biochemical changes in our modified semi-solid culture system. Several morphological changes observed in the primitive erythroid cell cultures, such as nuclear condensation, approach of nuclei to the periphery of cells, development by cells of an extended pear-like shape, enucleation, and an increase in haemoglobin content, were quite similar to those of the terminal differentiation of fetal liver or adult bone marrow (definitive) erythroid cells. In addition, the transition of molecular species of haemoglobin from the embryonic to the fetal/adult pattern was also observed in our culture system. Thus we provide evidence, by the in vitro culture of yolk-sac erythroid cells, that primitive erythroid cells undergo terminal differentiation in a manner similar to that of definitive erythroid cells.
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    Development genes and evolution 200 (1991), S. 108-112 
    ISSN: 1432-041X
    Keywords: Tenascin ; Embryogenesis ; Feather germ ; mRNA ; In situ hybridization
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    Notes: Summary I have studied the distribution of tenascin and its transcript during feather germ morphogenesis using immunohistochemistry and in situ hybridization. Anti-tenascin staining is intense in the periphery of dermal core condensations in both the feather rudiment and bud. There is faint anti-tenascin immunoreactivity in the overlying epithelium, but the apex of the bud is unstained. The appearance of tenascin in the developing feather is transient, as no significant anti-tenascin staining can be detected in the feather shaft or follicle at later stages of development. In situ hybridization with a tenascin cDNA probe reveals tenascin mRNA in the epithelial placode of the feather rudiment and early bud. In contrast, tenascin mRNA is concentrated in the dermis in the late feather bud. Therefore, at the time when inductive events are taking place, the expression of tenascin flips from the epithelium overlying tenascin-rich mesenchyme to the mesenchyme itself.
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  • 48
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    Development genes and evolution 200 (1991), S. 95-103 
    ISSN: 1432-041X
    Keywords: Drosophila melanogaster ; Eggshell ; Micropylar canal ; Microtubules ; Paracrystalline structure
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    Topics: Biology
    Notes: Summary The micropylar apparatus (MA) inDrosophila melanogaster (Diptera: Drosophilidae) is a 25 μm long protrusion on the anterior pole of the eggshell, and it contains the 0.8 μm wide micropylar canal through which the spermatozoon penetrates the eggshell. The canal terminates in the paracrystalline structure thus forming the “pocket”. The MA is secreted during oogenesis by the micropylar follicle cells (border cells and peripheral cells). Morphogenesis of the micropylar canal starts before stage 11A, when two of the border cells form two thin extensions containing microfilaments and penetrate the paracrystalline structure. Microtubules found at the base of the extensions participate in the formation of two projections. Adjacent follicle cells secrete the chorionic part of the MA, whilst the two projections elongate and twist during stages 12 and 13. Microtubules run parallel to the long axis of the projections and probably are related to cellular elongation during the formation of the projections. The paracrystalline structure is composed of lamellae having a periodicity of about 50 nm, which is maintained possibly due to the lateral fibrils connecting the lamellae. These lamellae consist of small fragments secreted during stages 10–11 by the border cells. The paracrystalline structure and the spongy vitelline membrane are condensed after egg maturation.
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  • 49
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    Development genes and evolution 200 (1991), S. 113-116 
    ISSN: 1432-041X
    Keywords: Extracellular matrix ; Fibronectin ; Laminin ; Tenascin ; Stomach
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    Notes: Summary The mesenchymes of the two avian stomachs, the proventriculus (glandular stomach) and the gizzard (muscular stomach), exert different inductive influences on stomach epithelial morphogenesis and cytodifferentiation. To search for a molecular difference between these two mesenchymes, we have produced monoclonal antibodies directed against chick proventriculi and gizzards and have screened those that differently recognized proventricular and gizzard mesenchymes. Finally, we obtained one monoclonal antibody, T95, and characterized it immunohistochemically. T95 characteristically stains the mesenchymal region just under the gizzard epithelium from 6 days of incubation onward to about 10 days of incubation, while it stains proventricular mesenchyme only weakly during these stages. We also examined immunohistochemically the distribution of well-known extracellular matrix molecules, such as fibronectin, laminin and tenascin, and none of them showed the same localization as T95 antigen in proventricular and gizzard mesenchymes. These results indicate that T95 will be an interesting marker which distinguishes the proventricular and gizzard mesenchymes, at the time when they have different inductive ability.
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    Development genes and evolution 200 (1991), S. 117-117 
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  • 51
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    Development genes and evolution 200 (1991), S. 143-148 
    ISSN: 1432-041X
    Keywords: Cell lineage ; HRP ; FITC-dextran ; Cleavage ; Axis determination ; Hydroids
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    Topics: Biology
    Notes: Summary Blastomeres of two-cell, four-cell, and eight-cell embryos of Hydractinia echinata were injected with horseradish-peroxidase (HRP) or fluorescein isothiocyanate (FITC)-dextran. The fate of the descendants of the injected blastomeres was followed until the planula larva had developed. The results obtained after HRP or FITC-dextran injection were essentially the same. Blastomeres are equivalent up to the four-cell stage, i.e. half-blastomeres produce half of the ectoderm of the planula larva and quarter-blastomeres give rise to one quarter of the larval ectoderm. During normal embryogenesis, the larval anterior-posterior axis corresponds to the animal-vegetal axis of the zygote. Thus, the labelled areas of larvae consisting of the progeny of injected half or quarter blastomeres normally stretch along the larval anterior-posterior axis. Normally, material giving rise to anterior or posterior larval parts, respectively, is separated at the third cleavage. Irrespective of the type of experiment, the progeny of injected blastomeres always contributed to endoderm formation, i.e. in larvae resulting from injected embryos the endoderm was more or less uniformly labelled. Application of vital stains locally to the exterior of zygotes and following these markers through first and second cleavage, produced evidence that in the vast majority of cases, the second cleavage is meridional.
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    Development genes and evolution 200 (1991), S. 132-142 
    ISSN: 1432-041X
    Keywords: Pattern formation ; Neurogenic genes ; Achaete-scute gene complex ; Cell interations
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    Notes: Summary The role of the achaete-scute complex and extramacrochaetae, Notch, Delta, Enhancer of split and Hairless genes in chaeta patterning in Drosophila tergites was studied in genetic mosaics and in mutant combinations. The mutant phenotypes of different alleles of each gene can be ordered in characteristic topographical seriations. These seriations are related to the pattern of proliferation of histoblasts and the time of singularization of sensory organ mother cells from surrounding epidermal cells. Genetic mosaics of lethal alleles show that these genes are fundamentally involved in this singularization and subsequent differentiation. The study of mutant combinations of alleles of these genes reveals specific relationships of epistasis and synergism between them. The results suggest that spatial and temporal variations in achaete-scute complex functional products in cells, modulated by the activity of other genes involved in signal transduction, define the patterned differentiation of sensory organs in tergites.
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    Development genes and evolution 200 (1991), S. 208-212 
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    Keywords: Extracellular matrix ; Morphogenesis ; Stretch-activated channels ; Limb bone rudiments
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    Topics: Biology
    Notes: Summary This communication describes a new experimental model for the study of the role of the extracellular matrix (ECM) in morphogenesis. In a preceding paper (Markson et al. 1991) we demonstrated that isolated epiphyses from femora of 6-day-old chick embryos grow during the first days in organ culture almost as well as their intact counterparts. Heating femora for 1 h at 45.2° C caused complete cessation of growth and proteoglycan biosynthesis. When the cut surface of a heat-inactivated (HI) epiphysis was brought into apposition with the cut surface of a live epiphysis and the attached pair placed in organ culture, the HI epiphysis began to grow and reached almost the same size as its live partner. The different possible interpretations of this finding are discussed. When a HI epiphysis of a certain shape (from humerus) is attached to a live epiphysis of a very different shape (from femur) and the attached pair is cultured for 6–7 days, the typical resumption of growth can be observed and the HI epiphysis that doubled or tripled its size retains its original characteristic form. The possibility that the existing infrastructure of the cartilaginous ECM directs the pattern of deposition of newly synthesized ECM by the chondroblasts is discussed, and it is suggested that stretch-activated channels participate in a process by which cells sense the topography of their ECM.
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  • 54
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    Development genes and evolution 200 (1991), S. 213-222 
    ISSN: 1432-041X
    Keywords: Maternal protein ; Egg polarity ; Monoclonal antibody ; Xenopus ; Maturation
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    Topics: Biology
    Notes: Summary For analysing spatial distribution of maternal proteins in an amphibian egg, monoclonal antibodies specific to certain regions were raised. One monoclonal antibody was found (MoAB Xa5B6) which reacted specifically with the animal hemisphere of the mature Xenopus laevis egg. The maternal protein that reacted with the MoAb Xa5B6 was shown to be distributed asymmetrically along the dorso-ventral axis in the upper region of the equatorial zone of the fertilized egg. At late blastula stage, the antigen protein could be observed clearly in both the marginal zone and animal cap. It was localized predominantly in mesodermal and ectodermal cells of late neurula embryos. The Xa5B6 antigen accumulated during oogenesis. The distribution pattern of maternal protein was remarkably different in the developmental stages of the oocyte. The pattern in the mature oocyte was completely different from that of the immature egg in which the antigen was located in the radial striations of the oocyte cytoplasm. After maturation, the distribution pattern changed drastically to an animal-vegetal polarization and the striation labellings were no longer observed. By Western blot examination, it was confirmed that the amounts of antigen protein were constant during early embryogenesis and the mesoectoderm contained a greater amount of antigens than the endoderm at late blastula. The antibody detected two bands of approximately 70 × 103 and 30 × 103 Mr by Western blot analysis. The latter molecule may possibly be a degrading moiety of the former. The results were discussed in relation to establishment of animal-vegetal (A/V) and dorso-ventral (D/V) polarization at the molecular level.
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  • 55
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    Development genes and evolution 200 (1991), S. 223-229 
    ISSN: 1432-041X
    Keywords: Cell cycle ; Embryogenesis ; Insect ; Diapause ; Bombyx mori
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    Topics: Biology
    Notes: Summary In the silkworm, Bombyx mori, diapause occurs at a specific embryonic stage, i.e. after formation of the germ band with cephalic lobes and telson and sequential mesoderm segmentation. As long as the eggs are incubated at 25° C, cell divisions and morphological development of the embryos cease. To examine changes in percentage of embryonic cells in the G1, S and G2 phases during embryogenesis, nuclear fractions were isolated from embryos, stained with propidium iodide and then subjected to flow cytometric analysis. The percentages of embryonic cells in G1, S and G2 were 10, 35 and 55%, respectively, at the stage of formation of cephalic lobes, whilst 98% of cells were in G2 at diapause stage. After termination of diapause by acclimation at 5° C or by a combination of chilling and HCl, cell division resumed in the embryos. During this period, the cells rapidly entered S phase through G1 from G2, suggesting that their G1 phase was short. In eggs in which diapause was averted by HCl-treatment after incubation at 25° C for 20 h after oviposition, embryonic development proceeded continuously for 9.5 days at 25° C until hatching. Along with this development, the G1 fraction increased to levels of about 90%. These results indicate that embryonic cells are arrested in G2 at diapause and suggest that, concomitant with further embryonic development, cell cycles become slower in proportion to an increasing length of G1. Finally, most of the cells may be arrested in G1, while there is only a small fraction of cells continuously cycling.
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    Development genes and evolution 200 (1991), S. 234-236 
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    Keywords: Hydra ; Nerve cells ; Differentiation ; Tumour-promoting phorbol esters
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    Topics: Biology
    Notes: Summary The tumour-promoting phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) interfers with nerve cell production in Hydra when applied to the animals' culture medium. Precursor cells exposed to 0.2 nM TPA during the first half of their S-phase are prevented from differentiating into nerve cells. Precursor cells which start their S-phase following a treatment with TPA give rise to nerve cells. The frequency is higher than in untreated control animals.
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  • 57
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    Development genes and evolution 200 (1991), S. 249-255 
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    Keywords: Metamorphosis ; Hydractinia echinata ; Hydrozoa ; Lysophosphatidylcholine ; Phospholipase A2
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    Topics: Biology
    Notes: Summary Whilst the significance of the phosphoinositide cycle in the activation of developmental events by extra-cellular signals is well established, the involvement of the phosphatidylcholine (PC) cycle is a matter just emerging. In the present study, the metabolism of phosphatidylcholine in early metamorphosis of Hydractinia echinata (Coelenterata; Hydrozoa) was investigated by incubation of planula larvae with 3H-choline, extraction of the metabolites and isolation of the metabolites by thin-layer chromatography (TLC). Phosphatidylcholine (PC), lysophosphatidylcholine (LPC), acetylcholine and glycerophosphocholine were the labelled metabolites. Induction of metamorphosis did not stimulate an increased incorporation of choline into PC. In larvae preincubated with 3H-choline to a steady state level of incorporation, a significant transient elevation of the radioactive label in LPC was observed 90 min after addition of metamorphosis stimulating agents. LPC probably derived from PC by the action of a phospholipase A2 (PLA2). LPCs from bovine and soybean origin as well as isolated larval LPC did not influence metamorphosis. PLA2 from bee venom promoted Cs+-induced metamorphosis but did not influence phorbol ester-induced metamorphosis. The data suggest that a PLA2 is activated during metamorphosis. This PLA2 activation does not occur in those putative receptor cells which receive the primary external inducing stimulus but in the many larval cells which resume proliferation or differentiation in response to a second, internally propagated signal.
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  • 59
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    Development genes and evolution 200 (1991), S. 256-268 
    ISSN: 1432-041X
    Keywords: Pattern formation ; Regeneration ; Insect segment ; Abdominal histoblasts
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    Topics: Biology
    Notes: Summary In dipteran flies the adult abdominal epidermis is formed from small nests of diploid histoblast cells which spread out and replace the larval epidermis during metamorphosis. The pattern of nest outgrowth and fusion in Sarcophaga shows that the large dorsal hemitergite is normally formed by the two dorsal nests, the spiracle nest and part of the ventral nest (which also forms the hemisternite). By rotating the dorsal histoblast nests, we demonstrate that the adult segment border lies between the flexible ‘intersegmental membrane’ (ISM) and the naked anterior strip of tergite, the acrotergite. Deletion of histoblast nests often results in a corresponding deletion of adult structures, accompanied by enlargement of adjacent structures within the segment and in neighbouring segments. Pattern formation is not strictly coupled to cell division (as in imaginal discs), since the nests remaining after an ablation, in spreading to fill vacant areas, generate more cells and larger structures than normal. Nest deletions can also result in regeneration, with remaining nests forming additional structures in the dorsal-ventral or anterior-posterior axis of the segment. The deletion of strips of anterior and intersegmental larval epidermis without histoblasts results in the formation of double-posterior duplications of the adult hemitergite. Although these operations damage adjacent histoblast nests, several features of the results suggest that the duplications arise from the interaction (after healing) of histoblasts with larval cells which they would not normally encounter, leading to the intercalation of histoblast cells bearing intervening anterior-posterior positional values. A similar process of intercalation may occur in normal development, as the histoblasts spread from their local origins across the larval epidermal sheet, replacing the larval cells to form the entire epidermis of the adult segment.
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    Development genes and evolution 200 (1991), S. 269-276 
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    Keywords: Stem cells ; Nerve cells ; Differentiation ; Microenvironment
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    Topics: Biology
    Notes: Summary The role of the cellular environment on hydra stem cell proliferation and differentiation was investigated by introduction of interstitial cells into host tissue of defined cellular composition. In epithelial tissue lacking all non-epithelial cells the interstitial cell population did not grow but differentiated into nerve cells and nematocytes. In host tissue with progressively increased numbers of nerve cells growth of the interstitial cell population was positively correlated to the nerve cell density. In agreement with previous observations (Bode et al. 1976), growth of the interstitial cell population was also found to be negatively correlated to the level of interstitial cells present. The strong correlation between the growth of the interstitial cell population and the presence of interstitial cells and nerve cells implies that interstitial cell proliferation is controlled by a feedback signal from interstitial cells and their derivatives. Our results suggest that the cellular environment of interstitial cells provides cues which are instrumental in stem cell decision making.
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  • 61
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    Development genes and evolution 200 (1991), S. 277-288 
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    Keywords: Insect epithelia ; Developing junctions ; Epithelial differentiation ; Gap junctions ; Septate junctions ; Proliferative stem cells ; Periplaneta americana (Blattidae)
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    Notes: Summary The stages in the development of intercellular junctions have been followed in the mesenteric caecal cells of the cockroach midgut, where two types of mature cell, the columnar and the secretory, exist. ‘Nests’ of undifferentiated replacement cells occur at intervals along the basal lamina, consisting of central, dividing cells and peripheral semi-lunar cells; the former act as proliferative stem cells to give rise to either pre-columnar or pre-secretory cells. The semi-lunar cells are pre-columnar and produce an attenuated process which gradually projects up to the luminal surface, producing microvilli and a dense extracellular substance en route. Intercellular gap junctions appear between these maturing columnar cell borders first, while septate junctions differentiate later; these are assembled from two different sets of intramembranous particle which become organized into either plaques or rows in parallel alignment, possibly mediated by actin filaments and microtubules. The pre-secretory cells, which are much fewer in number, remain associated only with the basal lamina and never reach the lumen; they develop into one of three distinct mature secretory cell types which release their secretory product in different ways.
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  • 62
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    Keywords: Retinol ; Hydrocortisone ; Mucous metaplasia ; Transdifferentiation ; Epidermis
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    Notes: Summary Epidermal mucous metaplasia of cultured skin can be induced by treatment with excess retinol for several days (Fell 1957). In the induction of mucous metaplasia, retinol primarily affects the dermal cells and retinol-pretreated dermis can alter epidermal differentiation towards secretory epithelium (Obinata et al. 1987). In this work, we found that mucous metaplasia could be induced by culturing 13-day-old chick embryonic tarsometatarsal skin in medium containing retinol (20 μM) for only 8–24 h, followed by culture in a chemically defined medium (BGJb) without retinol or serum for 6 days. The application of cycloheximide together with retinol during the first 8 h of culture inhibited epidermal mucous metaplasia during subsequent culture for 6 days in BGJb, indicating that induction of a signal(s) in the dermis by excess retinol requires protein synthesis. However, the presence of 20 nM hydrocortisone (Takata et al. 1981) throughout the culture period did not inhibit retinol-induced epidermal mucous metaplasia of the epidermis. This indicates that a brief treatment of the skin with excess retinol determines the direction of epithelial differentiation toward secretory epithelium; this is a simpler in vitro system for the induction of epidermal mucous metaplasia than those established before.
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    Development genes and evolution 200 (1991), S. 297-299 
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  • 64
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    Keywords: Artemia ; Mesoderm ; Heat treatment ; Postembryonic segmentation ; Germ layer uncoupling
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    Notes: Abstract Taking advantage of the fact than segmentation inArtemia is largely a postembryonic process making it more susceptible to environmental influences, heat treatments ofArtemia newly-hatched nauplii were shown to induce a severe inhibition of mesodermal structures, without apparently affecting the corresponding ectodermal and endodermal derivaives. This inhibition was reversible and with enough time the missing mesodermal structures developed. These results indicate that ectoderm and endoderm development can proceed without neccessarily a concomitant mesodermal differentiation, which in turn can be largely uncoupled from that of the rest of the germ layer derivatives.
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  • 65
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    Development genes and evolution 200 (1991), S. 306-311 
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    Keywords: Cytoplasmic P-lacZ ; Enhancer-trapping ; Nuclear targeting
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    Notes: Summary The N-terminal 125 amino acids of theDrosophila P element transposase are necessary and sufficient for the nuclear localisation of a hybridlacZ gene product in most cell types of theDrosophila embryo. A P-lacZ enhancer-trap element lacking these residues is of use in visualizing the shapes of P-lacZ-expressing cells.
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  • 66
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    Development genes and evolution 200 (1991), S. 312-319 
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    Keywords: Chick embryos ; DNA injection ; β-galactosidase expression ; Viral promoter
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    Notes: Summary A plasmid construct containing the reporter gene,lacZ, under the control of the cytomegalovirus immediate early promoter, was injected into the germinal disc of fertilised chick ova. The distribution of cells expressing β-galactosidase was examined in the embryos after periods of from 3 h to 7 days in culture. β-galacto-sidase-positive cells were first observed at mid-cleavage (250–500 closed cells) in the centre of the blastodisc. After one day, they were prominent in large segments of the blastoderm and, at later stages, in proportionately smaller segments of the extra-embryonic membranes, notably in the endodermal layer. In the embryonic regions, positive cells were scattered in the vicinity of the primitive streak of most cultures, but after gastrulation they were present in the embryonic tissue of only 7% of surviving embryos. The results provide supportive evidence for transcriptional activity during the cleavage stages of avian development. They also confirm previous findings on the loss of exogenous DNA during the early development of the chick.
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  • 67
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    Keywords: Ionic currents ; Ca2−/Mg+ ATPase ; Molluscan embryo ; Mesoderm induction ; Calcium
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    Notes: Summary InLymnaea stagnalis, mesoderm induction occurs at the 24-cell stage, when the apical tip of the macromere 3D establishes a close contact with a number of micromeres. Via its tip, the macromere 3D is supposed to receive an inductive signal from the micromeres, resulting in the determination of the mesodermal stem cell 4d at the next division. In view of the possibility that transcellular ionic currents might somehow be involved, either in the processes that bring about this particular configuration of blastomeres or in the induction process itself, we mapped the electric field around the embryo during the 24-cell stage, using a vibrating probe. We detected a reversal of the current direction as compared to the uncleaved egg, whilst the polarity of the field along the animal-vegetal axis was maintained. We also mapped the localization of Ca2+-stimulated AT-Pase, an enzyme that drives the Ca2+-efflux from the cell. We found that this enzyme is localized exclusively along the cytoplasmic face of the apical plasma membrane of macromere 3D, and that its presence is restricted to the period from 110 to 135 min after the fifth cleavage, when there is close contact between macormere 3D and the micromeres. Since the localization of the Ca2+-stimulated ATPase coincides both in time and space with the induction of the mesoderm-mother cell, we suggest that localized calcium fluxes may play a role in this induction process.
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  • 68
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    Keywords: Oogenesis ; Oocyte polarity ; Accessory nuclei ; Hymenoptera
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    Notes: Summary The progressive establishment of anteroposterior and dorsoventral polarity in developing oocytes ofCosmoconus meridionator is described. In fully grown oocytes, the asymmetrical (polar) organization is apparent in the localization of the oocyte nucleus (germinal vesicle) and oosome, and in the uneven (graded) distribution of lipid droplets, yolk spheres and specific organelles termed accessory nuclei (AN). The latter structures occur preferentially within the anteroventral periplasm. The developmental significance of AN is discussed.
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  • 69
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    Development genes and evolution 200 (1991), S. 336-341 
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    Keywords: Hydractinia echinata ; Metamorphosis ; Pattern formation ; Protein synthesis
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    Notes: Summary In the marine colonial hydroidHydractinia echinata metamorphosis from the larval to the adult (polyp) stage is induced by various agents, including CsCI and dioctanoylglycerol (diC8). Induction is prevented when the inhibitors of protein synthesis cycloheximide or ementine were applied simultaneously with the metamorphosis-inducing agents. With diC8 treatment, the inhibitors caused most animals to transform into mosaics consisting of larval and polyp body parts instead of normal shaped polyps. In contrast, treatment with cycloheximide or ementine just before or after incubation with the metamorphosis-inducing agents did not prevent larvae from metamorphosis. No substantial quantitative changes in protein synthesis occur during induction of metamorphosis, however, the protein pattern is changed upon induction. The most prominent new polypeptides (25 and 73 kD) were observed when CsCI was used to trigger metamorphosis. In addition, both in CsCl- and in diC8-treated larvae, the synthesis of a new 23 kD protein occurred, whilst synthesis of others ceased (41 and 44 kD).
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    Development genes and evolution 200 (1991), S. 353-353 
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  • 71
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    Keywords: Polychaete ; Centrifugation ; Janus duplicitas ; Determination ; Cell lineage
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    Notes: Summary Early development ofPlatynereis dumerilii is characterized by an extremely constant cleavage pattern in which the volumes and cytoplasmic contents of the blastomeres show remarkably little variability (Dorresteijn 1990). In order to test the necessity of a precise partitioning of the cytoplasm, we have stratified the ooplasm by mild centrifugation (10 min at 300 g) after completion of meiosis but before first cleavage. The cytoplasm of the zygote stratifies randomly with respect to the pre-existing animal-vegetal axis, but first cleavage follows the animal-vegetal axis dividing the plasm before it has rearranged to its normal distribution. As usual, first cleavage is unequal in the majority of centrifuged eggs. Different sorts of cytoplasm are always distributed abnormally in comparison to normal two-cell stages. Under two circumstances this leads to the formation of double trunk structures in the young worm. Such ‘double monsters’ either originate from zygotes whose clear cytoplasm has been distributed equally to the two daughter blastomeres at first cleavage, or from unequal two-cell embryos whose larger blastomere cleaved equally at second cleavage forming blastomeres with equal lots of clear cytoplasm. Cell-lineage could be followed in an individual embryo of the latter category and showed the existence of two adjacent D-quadrants, giving rise to a double monster with a forked trunk. Embryos of the former category give rise to two opponent D-quadrants and double monsters with a four-sided trunk. As in the normal embryo, the amount of clear cytoplasm in a blastomere is positively correlated with the speed of its cell cycle, and endows the cell with D-quadrant developmental capacities as can be judged by the cleavage pattern.
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    Development genes and evolution 199 (1991), S. 387-396 
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    Keywords: Pc group ; Ultrabithorax ; Antennapedia ; Sex combs reduced
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    Topics: Biology
    Notes: Summary Members of thePolycomb (Pc) group of genes are required for the correct determination of segment identity, and are thought to be negative regulators of thebithorax andAntennapedia complexes. This hypothesis has been tested molecularly for only some members of thePc group. Here, we examine the distribution ofUltrabithorax (Ubx),Antennapedia (Antp), andSex combs reduced (Scr) proteins in the epidermis, central nervous system, and midgut of embryos homozygous for mutations in tenPc group genes. We show that zygotic loss of mostPc group genes causes ectopic expression ofUbx andAntp, but that there are differences in time and tissue-specificity. FivePc group mutations lack midgut constrictions and also exhibit ectopic or suppressedUbx expression and suppression ofAntp expression. Distribution ofAntp is upset earlier than distribution ofUbx in the central nervous system of everyPc group mutant affecting both genes. Loss of the zygotic products ofPolycomb, extra sex combs, andAdditional sex combs cause ectopic expression ofScr in epidermis, and allPc group genes exceptPsc have suppressedScr expression in the nervous system. These results are discussed with respect to the function of thePc group.
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  • 73
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    Keywords: Polarity ; Molluscan development ; Polar lobe ; Concanavalin A ; F-actin
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    Notes: Summary The organization of the surface of fertilizedNassarius reticulatus eggs was probed by investigating the effects of treatment with concanavalin A (Con A). This lectin causes abnormal polar lobe formation as well as inhibition of cleavage. At low concentrations of Con A (0.3–1.0 μg/ml) the polar lobe constriction becomes considerably elongated, whereas at higher concentrations (2.5–50 μ/ml) the position of the constriction undergoes an extreme shift towards the animal pole. In the latter case, the surface of the animal part of the egg forms large protrusions and folds. Con A also causes resorption of microvilli and disappearance of the extracellular layer covering these villi; this process starts at the vegetal pole and propagates towards the animal pole. These changes in surface architecture are associated with profound changes in the organization of filamentous (F-) actin as assessed by confocal laser scanning microscopy of NBD-phallacidin-labelled eggs. Divalent succinyl-Con A has the same effects on polar lobe formation and surface architecture as tetravalent Con A, but only at very high concentrations (100–200 μg/ml), indicating that Con A exerts its effects by cross-linkage of its binding sites. Experiments with cytoskeleton inhibitors (cytochalasin D, colchicine, and nocodazole) reveal that in Con A-treated eggs — as in untreated eggs — microfilaments, but not microtubules, are involved in the formation of the polar lobe constriction. The calcium ion channel blocker D600 affects neither normal nor Con A-induced abnormal polar lobe formation, which suggests that influx of external calcium is not required. In contrast, treatment with TMB-8, an antagonist of internal calcium release, prevents the formation of a polar lobe in both normal and Con A-treated eggs. Finally, eggs from which the polar lobe has been removed prior to Con A treatment show none of the effects described, whereas isolated polar lobes react similarly to intact eggs. These results suggest that binding of Con A to sites present at the vegetal pole of the egg is responsible for the observed effects of the lectin.
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  • 74
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    Keywords: Supernumerary outgrowths ; Dorsoventral limb axis ; Anuran ; Bufo bufo
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    Notes: Summary Contralateral grafts were performed on the larval limb buds of the anuranBufo bufo. The dorsoventral axis of 80 buds at stages IV or V was inverted. Ten tadpoles were used as controls. Fifty-two supernumerary structures developed, all of them in dorsal or ventral locations on the host stump. The majority (32 out of the 44 outgrowths with more than 3 toes) were normal limbs of stump handedness. However, the following abnormal structures were also observed: 2 double-posterior, 3 mixed-symmetric, and 7 undetermined cases. These results are in agreement with the predictions of a hierarchical polar coordinate model for epimorphic regeneration.
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    Development genes and evolution 199 (1991), S. 364-369 
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    Keywords: Mouse embryo ; Two-dimensional gel electrophoresis ; SPARC ; Osteonectin
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    Topics: Biology
    Notes: Summary SPARC (secreted protein acidic and rich in cysteine), also known as osteonectin and BM-40, is a secreted protein associated with a variety of embryonic and adult tissue and cell types, including placenta, parietal and visceral endoderm, certain epithelia (e.g. gut, skin, glandular epithelia), and regions of active chondrogenesis and osteogenesis. Although much is known concerning the tissue distribution of this protein, neither the time and location of its initial appearance nor its functions during embryogenesis have been clearly established. We identified the location of SPARC on two-dimensional protein gels. By using two-dimensional gel analysis of both pre- and post-implantation stage mouse embryos, we find that SPARC is initially synthesized between 3.5 and 4.5 days of embryogenesis. This is the earliest time during development at which synthesis of SPARC has been demonstrated. Inner cell masses isolated from 4.5 day blastocysts synthesize SPARC indicating that either primitive ectoderm, primitive endoderm, or both produce this protein. SPARC synthesis is also detectable in isolated trophoblast vesicles. Thus, SPARC is synthesized not only in placenta, parietal endoderm, and visceral endoderm, but in the precursors of these tissues as well. Examination of 7.5 day embryos reveals that SPARC is synthesized in isolated parietal yolk sac and in whole extraembryonic and embryonic regions. Relative to other proteins, synthesis of SPARC was most prevalent in the parietal yolk sac. The possible implications of SPARC synthesis as early as 4.5 days are discussed.
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    Development genes and evolution 199 (1991), S. 377-386 
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    Keywords: Retinoic acid ; Cartilage degradation ; Tissue culture
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    Topics: Biology
    Notes: Summary Cartilage cubes, prepared from the proximal epiphyses of neonatal rat humeri and consisting of cartilage tissue only, were cultured in the presence of retinoic acid. The retinoid induced the loss of metachromatic staining with toluidine blue, which correlates with the loss of proteoglycan, followed by tissue degradation processes resulting in a distinct reduction of the cartilage mass. Histologically, fibroblast-like cells appeared within chondrones, indicating a transformation of chondroblasts. Focal tissue degradation was observed after only 2 days. Electron microscopically, the clustered cells within the zone of tissue degradation were rich in various lysosomal structures indicating their lytic activity. Cycloheximide and EDTA completely blocked the retinoic acid effects suggesting that protein synthesis was required and that metalloproteinases may be involved in the degradation processes. In conclusion, with the new test system described here we demonstrated that cartilage cells themselves performed the tissue degradation induced by retinoic acid.
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  • 77
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    Keywords: Ionic currents ; Ca2+/Mg2+ ATPase ; Molluscan eggs ; Cell cycle ; Calcium
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    Topics: Biology
    Notes: Summary During the first four mitotic division cycles of Lymnaea stagnalis embryos, we have detected cell cycle-dependent changes in the pattern of transcellular ionic currents and membrane-bound Ca2+-stimulated ATPase activity. Ionic currents ranging from 0.05 to 2.50 μA/cm2 have been measured using the vibrating probe technique. Enzyme activity was detected using Ando's cytochemical method (Ando et al. 1981) which reveals Ca2+/Mg2+ ATPase localization at the ultrastructural level, and under high-stringency conditions with respect to calcium availability, it reveals Ca2+-stimulated ATPase. The ionic currents and Ca2+-stimulated ATPase localization have in common that important changes occur during the M-phase of the cell cycles. Minimal outward current at the vegetal pole coincides with metaphase/anaphase. Maximal inward current at the animal pole coincides with the onset of cytokinesis at that pole. Ca2+-stimulated ATPase is absent from one half of the embryo at metaphase/anaphase of the two- and four-cell stage, whereas it is present in all cells during the remaining part of the cell cycle. Since fluctuations of cytosolic free calcium concentrations appear to correlate with both karyokinesis and cytokinesis, we speculate that part of the cyclic pattern of Ca2+-stimulated ATPase localization and of the transcellular ionic currents reflects the elevation of cytosolic free calcium concentration during the M-phase.
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  • 78
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    Development genes and evolution 200 (1991), S. 149-155 
    ISSN: 1432-041X
    Keywords: Central nervous system ; Ecdysteroid-receptor ; c-fos oncoprotein ; Transcriptional activator ; Insecta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The presence of c-fos, a marker for cell activation, was investigated in cerebral neurons actively expressing ecdysteroid receptors during larval-pupal development in the tobacco hornworm, Manduca sexta. Colocalization was accomplished by ecdysteroid autoradiography using the tritiated high affinity 20-hydroxyecdysone agonist ponasterone A and immunocytochemistry with an antibody to a peptide sequence which is highly conserved in both human and murine c-fos. Immunoreactivity to a c-fos-like protein(s) was present in nuclei of many neurons of all the developmental stages examined. However, with the exception of the optic lobe, cells expressing nuclear ecdysteroid receptors were more immunoreactive than non-ecdysteroid-binding neurons. These data suggest that ecdysteroid-induced gene activation and translation may involve c-fos expression.
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  • 79
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    Development genes and evolution 200 (1991), S. 172-176 
    ISSN: 1432-041X
    Keywords: Drosophila ; Sxl expression ; fl(2)d ; Adult life
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Drosophila melanogaster, the gene Sex-lethal (Sxl) controls the processes of sex determination, dosage compensation, oogenesis and sexual behaviour. The control of Sxl is by alternative splicing of its primary RNA. We have identified a gene, female-lethal-2-d (fl(2)d), which is needed for the female-specific splicing of Sxl RNA and which also has a vital function independent of Sxl. Here we analyse other aspects of the gene fl(2)d. Specifically, we have analysed the effect of the temperature-sensitive mutation fl(2)d 1 on the viability of adult flies homozygous for this mutation. We have found that the viability of the mutant females is reduced, while that of the mutant males is not affected. In addition, the capacity of the mutant females to be inseminated is considerably reduced, whilst all the mutant males are able to inseminate females. These effects on females are suppressed by Sxl M1. However, the fat body cells of fl(2)d 1 homozygous females are able to synthesize yolk proteins at the restrictive temperature. We have also carried out, in males, a clonal analysis of fl(2)d 2, a mutation lethal in both sexes. We have found that the clones are fully viable. We conclude that the gene fl(2)d seems to be necessary during the adult life of females for the processes that require Sxl + activity. Moreover, the Sxl-independent vital function of fl(2)d seems to be required in both sexes only during larval development.
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  • 80
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    Development genes and evolution 200 (1991), S. 162-171 
    ISSN: 1432-041X
    Keywords: Retinoid ; Determination ; Muscle ; Cartilage
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    Topics: Biology
    Notes: Summary The time of determination of cartilage and skeletal muscle was studied by making chimeric grafts or explants of small tissue pieces from several stages of early chick or quail embryos. Chondrogenesis was assessed by histology or with antibodies directed against type II collagen or cartilage proteoglycan, while myogenesis was detected immunohistochemically with antibodies directed against 3 different muscle markers, including muscle myosin. Grafts from Hensen's node, primitive streak and segmental plate of donor embryos of Stage 3–5 (Hamburger and Hamilton) were transplanted under the ectoderm in the extraembryonic area of Stage 12 host embryos. In addition, explants and mesodermal cells were cultured on glass in DMEM+F12 medium supplemented with 10% FCS. The results showed that determined myogenic cells could first be detected in Hensen's node and the primitive streak at Stage 3+−4 and that they developed from mesodermal cells located between the epiblast and hypoblast. Myogenic cells also appeared in grafted and explanted segmental plate with or without notochord from Stage 5 embryos. On the other hand, cartilage cells only formed in grafted and explanted segmental plate that also contained notochord. RA (1 ng/ml) could induce the formation of cartilage cells in the explanted primitive streak without Hensen's node or notochord taken from Stage 3–5 embryos and could also promote the differentiation of myogenic cells in primitive streak from Stage 3 embryo. Thus RA can substitute for Hensen's node or the notochord in the induction of cartilage cells and has some stimulatory effects on the differentiation of myogenic cells. Additional evidence indicates that the hypoblast might play an “inductive” role in the formation of the notochord which may subsequently promote the differentiation of cartilage cells.
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  • 81
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    Keywords: Morphogenesis ; Cell-migration ; Clone ; Hatching ; Halibut
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    Topics: Biology
    Notes: Summary The halibut hatching gland (HG) cells are first observed as a cellular disc in front of the embryonic head around the midpoint of intra ovo development. The disc is subsequently transformed into a loop of increasing diameter as the HG cells migrate over the anterior part of the yolk sac. When the HG disc is transformed into a loop, the density of HG cells is highest at the migratory front. Some HG cells lag behind the migrating front at the early stages of HG development. At maturity, all cells are contained in a narrow belt which is about 10 cells wide. The HG belt structure consists of a monolayer of HG cells, and is maintained while the cells migrate between the two epidermal cell layers. Migration is halted about 2 days before normal hatching when the HG cells reach a destination at about a right angle to on the embryonic axis. Under the scanning electron microscope, the differentiating HG cells protrude as a ridge the yolk sac surface. The HG cells immunostain with antiserum to hatching enzyme when the HG is observed as a crescent structure around the embryonic head. By counting the number of immunostaining cells in composite photos of the entire yolk sac membrane, we found that the HG belt consists of approximately 2000 secretory cells at maturity. This cell number stays fairly constant throughout the period of HG cell migration. Accordingly, mitoses of the halibut HG cells have generally ceased prior to morphogenesis, and cytodifferentiation is already quite advanced when cell migration starts.
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  • 82
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    Development genes and evolution 200 (1991), S. 202-207 
    ISSN: 1432-041X
    Keywords: Extracellular matrix ; Morphogenesis ; Cartilage ; Femur rudiment ; Proteoglycan turnover
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    Topics: Biology
    Notes: Summary Turnover of extracellular matrix (ECM) proteoglycans was studied in chick cartilaginous femur rudiments grown in organ culture. Femora from six-day-old embryos showed nearly normal growth rates during the first few days in culture. By labeling the rudiment with 35S-sulfate or 14C-glucosamine, it was demonstrated that the cartilaginous ECM undergoes rapid turnover. It was also found that the metabolic fate of the proteoglycans is to be released as macromolecules into the culture medium. When a rudiment was cut to obtain two epiphyses it was observed that each part grows and synthesizes proteoglycans at nearly normal rates, which indicates that the isolated epiphyses, like the whole rudiment, behave as autonomous systems. We suggest that the turnover of ECM components is part of the continuous remodelling process rudiments undergo during their growth and development. In order to study cell-ECM interaction in morphogenesis, we made an attempt to prepare an intact cell-free ECM. Epiphyses were heated at 45.2° C for 1 h. The treatment caused complete cessation of growth and biosynthesis. When the cut surface of a live epiphysis was brought into apposition to a heat-treated epiphysis and the attached pair placed in organ culture, it was found that the heat-treated epiphysis begins to grow and reaches almost the same size as its live counterpart. We discuss the possible advantage of this new experimental system for studies on the role of ECM in morphogenesis.
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  • 83
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    Development genes and evolution 200 (1991), S. 193-201 
    ISSN: 1432-041X
    Keywords: Ascidian anural development
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    Topics: Biology
    Notes: Summary Tadpole development is eliminated in the life cycle of the ascidian Molgula pacifica. The elimination of a tailed larva is termed anural development, in contrast to urodele development which is exhibited by most ascidian species. In the present study, transmission electron microscopy and histochemistry were used to gain a better understanding of anural development in M. pacifica. The fine structure of M. pacifica oocytes and fertilized eggs was similar to urodele oocytes and eggs, except that a perivitelline space and test cells were absent. M. pacifica embryos exhibited the typical cleavage pattern of urodele embryos. Gastrulation was initiated at the vegetal pole, as in urodeles, and occurred at the same time as in two urodele species (Molgula manhattensis and Pyura haustor). However, changes in cell shapes and cell movements of the vegetal pole cells that participate in gastrulation were highly modified compared to commonly studied ascidians. The changes in shapes and movements of the vegetal pole cells were minimal and resulted in embryos having a very small archenteron and blastopore. The presence of large, yolky cells in the interior of the embryo likely restricted vegetal cell movements. Two ultrastructurally distinct types of epidermal cells were evident at the gastrula stage. When gastrulae were manually dechorionated from their surrounding mucous-follicular envelope layers, the embryos were already surrounded by a thin tunic. When day 1 juveniles in the process of hatching were sectioned along the anterior-posterior axis, regional differences in cell types were evident. Differentiated muscle cells in the posterior region were not evident. Day 1 M. pacifica juveniles, anural-developing M. provisionalis juveniles and tadpoles from three urodele species were tested for their abilities to express AchE activity. The highest levels of AchE activity were detected in the larval tail muscle cells of urodeles, low levels of activity were detected in the posterior region of M. provisionalis juveniles, whereas M. pacifica juveniles did not exhibit AchE activity. The results are discussed in terms of evolutionary mechanisms responsible for anural development in ascidians.
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  • 84
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    Development genes and evolution 200 (1991), S. 188-192 
    ISSN: 1432-041X
    Keywords: Myogenesis ; Migration ; Muscle ; Vital staining ; Limb morphogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to understand how myogenic cells migrate in the limb bud, it is indispensable to distinguish undifferentiated myogenic cells from other mesenchymal cells. Thus, a suitable method for this purpose has been sought. A method to exchange the somites of a chicken and a quail microsurgically has widely been used, since the nuclei of the two species are morphologically distinguishable. However, microsurgery is accompanied by disturbances at the operated locus, and introducing cells of different species might induce unexpected effects. We report a new method for labelling chicken myogenic cells without transplantational operations, and describe their migration pattern in limb buds. Injection of a fluorescent carbocyanine dye into the somite lumen intensely labelled the somitic cells. Myogenic cells derived from the somite were clearly detected in limb buds. Before stage 20, the labelled cells were diffusely distributed in the proximal region of the limb bud. At about stage 21 in both wing and leg buds, labelled cells began to form dorsal and ventral masses. The label was followed until the cells differentiated and expressed myosin. This vital labelling method has advantages over the somite transplantation method: it does not include surgical operations that may disturb the normal development, and the cells are labelled intensely enough to be detected in a whole mount preparation.
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  • 85
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    Development genes and evolution 199 (1991), S. 327-334 
    ISSN: 1432-041X
    Keywords: Insect neurodevelopment ; Insect embryogenesis ; Invertebrate development ; Developmental entomology ; Lepidopteran development ; Manduca sexta
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    Topics: Biology
    Notes: Summary A complete timetable of embryonic development of the tobacco hawkmoth,Manduca sexta (Lepidoptera: Sphingidae), is presented. Using living embryos, 20 developmental stages from oviposition to hatching are described with respect to their morphological and physiological maturation. This staging series provides a simple method to identify the stage ofManduca development during all phases of embryogenesis.
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  • 86
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    Keywords: Drosophila oogenesis ; Culture media ; Ionic current patterns ; Membrane potentials ; Intrafollicular transport
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    Topics: Biology
    Notes: Summary Contradictory electrophysiological results were recently obtained inDrosophila ovarian follicles kept in different salines or complete media during measurements. Therefore, I checked follicles maintained in various solutions using morphological, physiological and biochemical criteria. Defined complete media were the best for supporting development from stage 10 to stage 14 (end of oogenesis). Supplementation of the solutions with insect pupal haemolymph had negative effects. For the maintenance of RNA synthesis, complete media were again superior to simple salines. Total protein synthesis was not very sensitive to the culture conditions during short incubation periods, but electrophoretic protein patterns were slightly less complex in the salines than in complete media. Furthermore, some major proteins (e.g. chorion proteins) synthesized during longterm culture failed to appear in the salines. In view of extrafollicular electrical currents and intracellular electrical potentials, potassium uptake experiments were conducted with several inhibitors, using rubidium-86 as a probe. Both potassium concentration and osmolarity were found to exert strong influences on total potassium uptake of the follicles. In the tested media and salines differing amounts of potassium were taken up via (Na+, K+)-ATPase, via other K+-pump(s) or passively. The possible influences of several parameters on the outcome of earlier in vitro experiments withDrosophila follicles are discussed. For further in vitro studies the chemically defined complete R-14 medium seems to be the most suitable.
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    Development genes and evolution 199 (1991), S. 341-348 
    ISSN: 1432-041X
    Keywords: Xenopus ; Organizer ; Late Blastula ; Notochord ; Induction
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Summary One important step in understanding early development is to define the cell interactions involved in establishing tissue types. In amphibian embryos, one such interaction is the induction by the organizer region after the late blastula stage of lateral and ventral regions of the marginal zone (MZ) to form dorsal tissue types such as muscle. It is not known whether the organizer can also induce lateral MZ to form notochord after the late blastula stage. We find that this induction occurs under experimental conditions and plays a role in normalXenopus development. The ability to induce notochord is strongest at the center of the organizer along the dorsal midline and weaker at the lateral edges of the organizer. Organizer tissue along the dorsal midline, which would differentiate as notochord in normal development, can exhibit organizer functions such as the induction of the dorsolateral MZ to form notochord without later differentiating as notochord itself. Thus organizer activity can be dissociated from subsequent notochord formation.
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    Development genes and evolution 199 (1991), S. 335-340 
    ISSN: 1432-041X
    Keywords: Cell migration ; Induction ; Extracellular matrix ; Monensin ; Chick embryo
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    Notes: Summary In early chick blastoderm at stage XIII, the interaction of the hypoblast with the epiblast triggers on the epiblast the first extensive cellular migrations, which result in formation of the primitive streak, the source of the axial mesoderm. During this period, extracellular material (ECM) is secreted and assembled into an organized network in the extracellular spaces and is implicated in regulating the behaviour of the cells that contact it. The first cellular migrations and inductions are inhibited when early chick blastoderm is treated with the glycosylation-perturbing ionophore monensin. The difference in amount and in organization of ECM between monensin-treated embryos and control embryos is striking. Even blastoderms at stage X, which are essentially free of ECM, show extensive ECM after monensin treatment. Monensin produces a substantial change in the polypeptide pattern with the induction or marked accentuation of multiple charged species (isoforms) of polypeptides different from those present in the control embryos. The interference of monensin with the migration and induction mechanisms is permanent in embryos before the primitive streak (PS) stage, and it seems that the respective signals or the sensitivity of the epiblast/hypoblast cells to them must be very stage specific. Monensin-treated embryos probably secrete abnormal ECM that does not provide the proper conditions for the hypoblast to interact with the epiblast cells.
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    Development genes and evolution 199 (1991), S. 349-363 
    ISSN: 1432-041X
    Keywords: Centipedes ; Myriapod embryogenesis ; Neurogenesis ; Segmentation ; Axon growth
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    Topics: Biology
    Notes: Summary We have examined the embryo of the centipedeEthmostigmus rubripes to determine the degree of evolutionary conservatism in the developmental processes of segmentation, neurogenesis and axon formation between the insects and the myriapods. A conspicuous feature of centipede embryogenesis is the early separation of the left and right sides of the ganglionic primordia by extra-embryonic ectoderm. An antibody to the protein encoded by theDrosophila segmentation geneengrailed binds to cells in the posterior margin of the limb buds in the centipede embryo, in common with insect and crustacean embryos. However, whereas in insects and crustaceans this protein is also expressed in a subset of cells in the neuroectoderm, the anti-engrailed antibody did not bind to cells in the ganglionic primordia of the centipede embryo. Use of the BrdU labelling technique to mark mitotically active cells revealed that neuroblasts, the ubiquitous neuron stem cell type in insects, are not present in the centipede. The earliest central axon pathways in the centipede embryo do not arise from segmentally repeated neurons, as is the case in insects, but rather by the posteriorly directed growth of axons originating from neurons located in the brain. Axonogenesis by segmental neurons begins later in development; the pattern of neurons involved is not obviously homologous to the conservative set of central pioneering neurons found in insects. Our observations point to considerable differences between the insects and the myriapods in mechanisms for neurogenesis and the formation of central axon pathways, suggesting that these developmental processes have not been strongly conserved during arthropod evolution.
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    Development genes and evolution 199 (1991), S. 373-376 
    ISSN: 1432-041X
    Keywords: Mitotic domains ; Blastoderm ; Musca domestica
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    Topics: Biology
    Notes: Summary We describe the mitotic cleavage patterns during blastoderm stage of the house flyMusca domestica L. Nuclear divisions up to mitotic stage 11 are apparently synchronous. Beginning with stage 12, nuclear divisions in the posterior third of the embryo lag behind, resulting first in a parasynchronous and finally in an asynchronous cleavage pattern. Thus a stage exists where all nuclei in the anterior region have completed 14 nuclear division cycles, while those in the posterior region have completed only 13 cycles. The border region between these nuclei is well defined and lies at 35% EL (egg length), the expression border of a gap gene. This border region is about 4–5 nuclei wide and shows a specialized mitotic behaviour.
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  • 91
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    Development genes and evolution 199 (1991), S. 370-372 
    ISSN: 1432-041X
    Keywords: Metamorphosis ; Hydractinia echinata (Hydrozoa) ; Metamorphosis inducing factor
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    Topics: Biology
    Notes: Summary A metamorphosis-inducing factor was isolated from medium conditioned by either metamorphosing larvae or 3-day postmetamorphic primary polyps. The factor has a molecular weight ≥ 8 kDa and is heatlabile. It does not induce metamorphosis of isolated posterior fragments and is therefore not identical to the internal signal described by Schwoerer-Böhning et al. (1990). The biological significance of the substance is currently unclear, therefore its inducing activity may be a side effect.
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  • 92
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    Development genes and evolution 199 (1991), S. 409-412 
    ISSN: 1432-041X
    Keywords: Neural induction ; Mesodermalization ; Amphibia ; Protein synthesis ; Glycosylation
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    Topics: Biology
    Notes: Summary An artificially mesodermalized ectoderm (mE) shows the same properties as the organizer: chordamesoderm formation and neural induction. The neural-inducing activity of the mE was inhibited by treatment with protein synthesis inhibitors (cycloheximide and puromycin) and a specific inhibitor of protein glycosylation (tunicamycin). These antibiotics also inhibited chordamesoderm differentiaton, especiallly that of notochord. Newly synthesized proteins of the mE were compared with those of presumptive ectoderm (pE) using two-dimensional PAGE. There were differences in relative amounts of many protein spots. These results suggest that neural-inducing activity is related to glycoproteins synthesized during the early phase of mesodermalization.
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  • 93
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    Development genes and evolution 199 (1991), S. 423-426 
    ISSN: 1432-041X
    Keywords: Oogenesis ; Accessory nuclei ; Developmental gradients ; Ultrastructure
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    Notes: Summary In the oocytes ofTenthredo olivacea, accessory nuclei (AN) are formed by budding from the nuclear envelope of the oocyte nucleus. Newly formed AN contain electron-dense material of nuclear origin and are surrounded by a double envelope devoid of pores. Such structures are subsequently transported to the peripheral ooplasm (periplasm), where they grow to reach a final diameter of 5 µm. In the envelopes of advanced AN nuclear pores arise. Through these pores “nuage” material is extruded into the surrounding periplasm. These findings are discussed with respect to a possible involvement of AN in the establishment of developmental gradients in hymenopteran oocytes.
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    Development genes and evolution 199 (1991), S. 469-475 
    ISSN: 1432-041X
    Keywords: Insect embryo ; Bombyx mori ; Arrest in diapause ; Protein pattern ; Heat-shock
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    Topics: Biology
    Notes: Summary Two-dimensional protein gels are used to assess systematically changes in protein synthesis in diapausing and non-diapausing early embryos ofBombyx mori throughout natural breakage of diapause by chilling and after artificial prevention of diapause by HCl+ heat-shock treatment. A set of proteins, the heat-shock protein (hsp) 70 family previously described, was synthesized in diapausing and non-diapausing development at the early germ-anlage stage; by contrast, protein 61 (P61; 61 kDa) was synthesized only in the diapausing gastrula stage. The synthesis of P61 decreased during days at 5°C. Thereafter, we observed the synthesis of the hsp 70 family at 5°C. After the artificial prevention of diapause, P61 synthesis was not induced at the gastrula stage, whereas the hsp 70 family was enhanced in the 6 h following the preventive treatment. P61 was only synthesized after treatments were ineffective in preventing diapause and in inducing hsp 70. Thus, P61 can be described as a “diapause arrest-associated protein.”
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    Development genes and evolution 199 (1991), S. 493-493 
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  • 96
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    Keywords: Oocyte nuclear proteins ; Lampbrush chromosomes ; Monoclonal antibody ; Development ; Pleurodeles waltl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The distribution of a nuclear antigen ofPleurodeles waltl oocytes, recognized by the monoclonal antibody B24/1, has been studied during oogenesis and early embryonic development. In stage I oocytes the antigen was localized in the nucleoplasm and on two atypical structures of lampbrush chromosomes, the spheres (S) and the mass (M). The immunostaining increased as the oocyte developed. In stage VI oocytes, the nucleoplasm and spheres showed intense staining. At this stage, the nucleoplasm often contained free spheres which were also labelled. The staining of M diminished during oogenesis, as did its size. Immunoblots of nuclear proteins of oocytes at different stages confirmed that there was an accumulation of this protein during oogenesis. During embryonic development, the nuclei of all the cells of blastula and gastrula were labelled by this antibody: there was no embryonic regionalization. Starting from the neurula stage, the staining progressively disappeared from the nuclei of ectodermal and mesodermal cells. In the tailbud stage, only the endodermal cell nuclei showed faint staining. Immunoblots of proteins from embryos of different stages showed that the quantity of this protein was constant until the young gastrula stage and then decreased progressively; in the young tailbud stage, this protein was practically absent. B24/1 is the first described protein of the sphere. This protein is accumulated in the oocyte nucleus and behaves like a maternal polypeptide, shifting early in the nuclei during embryonic development. Thus, B24/1 probably has a function required from the early developmental stages, perhaps in relation with small nuclear ribonucleoproteins.
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    Development genes and evolution 199 (1991), S. 476-491 
    ISSN: 1432-041X
    Keywords: Pattern formation ; Insect leg segmentation ; Cell surface antigen ; Monoclonal antibody ; Prepattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A monoclonal antibody (MAb) that binds to a cell surface antigen selectively localized to epithelial cells undergoing morphogenesis was used to study the segmentation of the growing embryonic leg of the cockroachPeriplaneta americana. The MAb labels circumferential stripes of cells at locations where invagination will occur to form the leg segments. The formation of these stripes precedes any morphological change in the epithelial layer or in individual cells. The temporal and spatial distribution of the antigen indicates the existence of a prepattern for leg segmentation, examination of which can give information about pattern generating mechanisms. Although highly stereotyped, the sequence in which the stripes appear does not follow a simple pattern proceeding in one direction along the proximal-distal axis. It is proposed that each stripe is a boundary in a positional field. Stripe formation leads to the division of the leg into a repeating series of identical positional fields. Three different mechanisms for the formation of stripes of MAb labeled cells have been observed and the role of each in the evolution of the insect leg is discussed. Measurements of leg and leg segment lengths when the various stripes appear has demonstrated considerable variation, particularly at the early stages of segmentation. Rules or mechanisms generating pattern at early stages of development are not rigid. Variations arising are compensated for by later occurring events so that stereotyped structures are formed.
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    Insectes sociaux 38 (1991), S. 17-25 
    ISSN: 1420-9098
    Keywords: Trail pheromone ; Tapinoma simrothi ; pygidial glands ; iridodials
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pygidial (anal) gland was found to be the source of trail pheromone in the antTapinoma simrothi. Bioassays conducted with fractionated pygidial gland secretion indicated that the fraction containing iridodials and iridomyrmecin is responsible for the trail pheromone activity. Thus workers ofT. simrothi may utilize the same glandular exudate for alarm and trail following. At high emission rates from a point source, the ants responded in alarm, e.g., rushed to the source with open mandibles and raised abdomen. When concentrations were low and drawn as a line, the ants followed the secretion calmly. Trails ofT. simrothi are long-lived, having a biological half-life of 10 to 19 days. Quantitative studies of the evaporation rates of the iridodials by gas chromatography resulted in a half-life of 11 days, agreeing with the biological data. The implications of the use of the same glandular secretion for alarm and food recruitments are discussed.
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    Insectes sociaux 38 (1991), S. 45-49 
    ISSN: 1420-9098
    Keywords: Ant ; Cataglyphis bicolor ; odd behaviour
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    Topics: Biology
    Notes: Summary An unexplained form of behaviour connecting nests of an antCataglyphis bicolor F. is described. Individuals from one nest visit another wandering around the nest entrance, for periods of up to half an hour or more. Their behaviour is quite distinct from any seen in normal foraging. These individuals come from the upper end of the range of size; more frequently but not always from a larger to a smaller nest. Nests are normally antagonistic. These ants back away rapidly from an ant of the nest visited.
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  • 100
    ISSN: 1420-9098
    Keywords: Eggs ; queens ; microgynes ; behaviour ; Myrmica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. The results are described for three experiments investigating the grouping, in small observation nests, of queens of the antMyrmica rubra L. 2. The first describes how a group of normal queens, without workers, establishes an egg-mass which acts as a core. Then when workers are introduced, how they gradually take over the general duties of the colony while the queens' activities are concentrated in the core. 3. The second describes the behaviour of a large natural group of microgynes and workers in the observation nest. It shows that microgynes, like normal queens, establish a core. Reduction in the worker population does not seriously affect the colony's structure. 4. The third experiment describes what happens when normal queens and microgynes are grafted (a process of gradual introduction) into small colonies of the other type. Contrary to expectation the newly grafted queens, of whichever type, monopolized the core at the expense of the familiar queens. 5. The importance of the core for the activity and breeding success of queens is reviewed and the results of the three observation experiments are discussed in the light of this review. Especial emphasis is given to the social status of microgynes within colonies in relation to the alternative views: that they are either true social parasites or some form of balanced polymorphism.
    Type of Medium: Electronic Resource
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