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  • Articles  (119)
  • monoclonal antibody  (47)
  • serum-free medium  (37)
  • Bacteria  (30)
  • Lepidoptera
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  • Springer  (119)
  • Process Engineering, Biotechnology, Nutrition Technology  (119)
  • 1
    Electronic Resource
    Electronic Resource
    Cadmium sorption by bacteria and freshwater sediment (1991)
    Springer
    Journal of industrial microbiology and biotechnology 8 (1991), S. 201-207 
    Details
    ISSN: 1476-5535
    Keywords: Diffusion chamber ; Cadmium-sensitive ; Cadmium-resitant ; Sediment ; Bacteria ; Cadmium-sorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Sorption of cadmium by sediment bacteria and freshwater sediment was investigated using diffusion chambers to simulate the water-sediment interface. Diffusion chambers were constructed to provide two compartments separated by a dialysis membrane. Diffusion of cadmium across the membrane was monitored after pure cultures of sediment bacteria or lake sediments were added to the sediment side of a diffusion chamber. Cellular accumulation of cadmium by cadmium-sensitive and cadmium-resistant bacteria removed between 20% and 80% of the dissolved cadmium from the simulated water column and pore water. Cellular accumulation of cadmium was greatest for cadmium-sensitive isolates that were tested. Sediment with an intact microbial community sequestered 80% of the cadmium added to sediment, whereas autoclaved sediment retained 97% of the metal that was added. Addition of glucose to cadmium-amended sediment decreased retention of cadmium by untreated and autoclaved sediments, resulting in elevated concentrations of dissolved cadmium in the simulated water column.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01575854
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  • 2
    Electronic Resource
    Electronic Resource
    Oligotrophic bacteria in ultra-pure water systems: Media selection and process component evaluations (1991)
    Springer
    Journal of industrial microbiology and biotechnology 8 (1991), S. 223-227 
    Details
    ISSN: 1476-5535
    Keywords: Deionized water ; Ultra-pure water ; Ozone ; Ultra-violet sterilization ; Oligotroph ; Bacteria ; R2A medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Presently, tryptic soy agar (TSA) medium is used in the semiconductor industry to determine the concentration of viable oligotrophic bacteria in ultra-pure water systems. Deionized water from an ultra-pure water pilot plant was evaluated for bacterial growth at specific locations, using a non-selective medium (R2A) designed to detect injured heterotrophic as well as oligotrophic bacteria. Results were compared to those obtained using Tryptic Soy Agar. Statistically greater numbers of bacteria were observed when R2A was used as the growth medium. Total viable bacterial numbers were compared both before and after each treatment step of the recirculating loop to determine their effectiveness in removing bacteria. The reduction in bacterial numbers for the reverse osmosis unit, the ion exchange bed, and the ultraviolet sterilizer were 97.4%, 31.3%, and 72.8%, respectively, using TSA medium, and 98.4%, 78.4%, and 35.8% using R2A medium. The number of viable bacteria increased by 60.7% based on TSA medium and 15.7% based on R2A medium after passage of the water through an in-line 0.2-μm pore size nylon filter, probably because of the growth of bacteria on the filter. Our results suggest that R2A medium may give a better representation of the microbial water quality in ultra-pure water systems and therefore a better idea of the effectiveness of the various treatment processes in the control of bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01576059
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  • 3
    Electronic Resource
    Electronic Resource
    Bacterial conjugation betweenEscherichia coli andPseudomonas spp. donor and recipient cells in soil (1990)
    Springer
    Journal of industrial microbiology and biotechnology 5 (1990), S. 79-84 
    Details
    ISSN: 1476-5535
    Keywords: Bacteria ; Soil ; Conjugation ; Gene transfer ; Plasmids ; Survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Experiments conducted in microcosms containing loam soil samples inoculated with eitherE. coli orPseudomonas spp. donor and recipient cells showed that bacterial cells survived and conjugated over a 24-h incubation period.E. coli transconjugants were detected 6 h after donor and recipient strains were introduced into sterile soil samples. In non-sterile soil samples, transconjugants were detected between 8 and 24 h incubation.Pseudomonas transconjugants were recovered from sterile soil samples between 6 and 12 h after their introduction and as early as 2 h in non-sterile soil. The results show that genetic interactions occur in non-sterile soil in relatively short periods of time at relatively high transfer frequencies (10−3 to 10−4). Studies on genetic interactions in soil are becoming necessary in risk assessment/environmental impact studies prior to the release of genetically engineered or modified organisms into uncontained environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01573856
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  • 4
    Electronic Resource
    Electronic Resource
    Production of extracellular emulsifying agent byPseudomonas aeruginosa UG1 (1990)
    Springer
    Journal of industrial microbiology and biotechnology 5 (1990), S. 25-31 
    Details
    ISSN: 1476-5535
    Keywords: Oil ; Emulsifier ; Bacteria ; Pseudomonas aeruginosa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Twenty-three bacterial strains were isolated from oil-contaminated soil samples. Of these, 20 displayed some ability to effect oil dispersion and they were screened quantitatively for the ability to emulsify 0.5% (v/v) reference oil. One strain, identified asPseudomonas aeruginosa UG1, produced extracellular material that emulsified reference oil, hexadecane and 2-methylnaphthalene at concentrations as high as 6% (v/v) in nutrient broth. Emulsification activity increased during a 10 day incubation period at 30°C. The activity was not influenced by pH over the range 5 to 9. The emulsifying agent was precipitated by cold ethanol. The highest emulsifying activity was detected in the extracellular fraction precipitated between 30 and 50% (v/v) ethanol. A linear relationship was observed between emulsifier concentration (mg/ml) and emulsifying activity. Genetic analysis showed that thePseudomonas aeruginosa UG1 strain did not carry extrachromosomal plasmids, suggesting that the gene(s) coding for emulsifying activity was carried on the chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569603
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  • 5
    Electronic Resource
    Electronic Resource
    Bacterial species in raw and cured compost from a large-scale urban composter (1994)
    Springer
    Journal of industrial microbiology and biotechnology 13 (1994), S. 177-182 
    Details
    ISSN: 1476-5535
    Keywords: Antibiotic resistance ; Bacteria ; Compost ; Metal-resistance ; Thermophilic bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Raw and cured compost samples from a large-scale urban composter were studied over a period of eight months to gain information on bacterial species present. Total viable, aerobic heterotrophic bacteria, lactose-positive bacteria, antibiotic and metal-resistant bacteria and thermophilic bacteria were enumerated. Both raw and cured compost samples contained metal and antibiotic-tolerant bacteria (〈Log 3.0 to Log 8.5 CFU g−1 compost) as well as high numbers (as high as Log 7.4 CFU g−1 dry weight compost) of thermophilic bacteria isolated by growth at 55 °C. Selected colonies were also identified using the Biolog 95 substrate identification system.Escherichia coli andSalmonella spp. were not detected in compost samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01584004
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution of plasmids in groundwater bacteria (1987)
    Springer
    Journal of industrial microbiology and biotechnology 1 (1987), S. 311-317 
    Details
    ISSN: 1476-5535
    Keywords: Plasmid ; Groundwater ; Aquifer ; Bacteria ; Contamination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Bacteria isolated from groundwater aquifer core materials of pristine aquifers at Lula and Pickett, OK, and from a site with a history of aromatic hydrocarbon contamination and natural renovation located at Conroe, TX, were screened for the presence of plasmid DNA by alkaline or enzyme lysis and agarose gel techniques. Some of the isolates were also subjected to taxonomic tests in addition to screening for resistance to antibiotics, tolerance to heavy metal salts, and bacteriocin production. There was no significant difference in the distribution of the traits usually associated with plasmid occurrence in isolates from the three sites. These traits, which occurred at low frequencies, were not restricted to plasmid-bearing strains of the communities. Plasmids were found in isolates from all three sites, but on the average there was a significantly higher percentage of isolates containing plasmids in the samples from Conroe (19.4%) than from either Lula (1.8%) or Pickett (7.7%). The sizes of the plasmids found ranged between 3.5 and 202 kilobases but, for the Conroe samples, many more isolates (67%) contained smaller plasmids (〈10 kb) rather than larger ones. No plasmids were found in bacteria recovered from naturally renovated aquifer material at the Conroe site.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569309
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  • 7
    Electronic Resource
    Electronic Resource
    Solubilization of coal by an extracellular product fromStreptomyces setonii 75Vi2 (1987)
    Springer
    Journal of industrial microbiology and biotechnology 1 (1987), S. 371-375 
    Details
    ISSN: 1476-5535
    Keywords: Coal ; Coal solubilization ; Streptomyces ; Bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Several low-ranked coals were solubilized when placed on the surface of agar cultures ofStreptomyces viridosporous T7A andS. setonii 75Vi2. When grown in submerged cultureS. setonii 75Vi2 produced an extracellular component that was capable of solubilizing coals. The extracellular coal solubilizing component had a molecular weight of 〈10000 and was heat stable since, after 1h at 121°C, only 30–40% of the activity was lost. Treatment with any of three proteases also appeared to be ineffective in decreasing activity. These results suggest that coal solubilization byS. setonii 75Vi2 is nonenzymatic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569334
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  • 8
    Electronic Resource
    Electronic Resource
    Production of monoclonal antibodies and ELISA for thebaine and codeine (1995)
    Springer
    Cytotechnology 19 (1995), S. 55-61 
    Details
    ISSN: 1573-0778
    Keywords: codeine ; ELISA ; monoclonal antibody ; opium alkaloid ; qualitative analysis ; thebaine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The ratio of hapten and bovine serum albumin in antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. Monoclonal antibodies against thebaine and codeine were produced by hybridoma fused with the sprenocytes immunized with thebaine- and codeine-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. No cross-reaction of anti-thebaine antibody against morphine was observed. Very small cross-reaction appeared in codeine (0.004%). The cross-reaction of anti-codeine antibody against morphine and thebaine was 2.97 and 5.98%, respectively. The full measuring range of the assay extends from 60 pg mL to 1 ng mL for thebaine and 1 ng mL to 100 ng mL for codeine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749755
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  • 9
    Electronic Resource
    Electronic Resource
    Stabile production of a thrombin resistant pro-urokinase derivative (PRO-UKS1) by Namalwa KJM-1 cells adapted to serum-free medium (1995)
    Springer
    Cytotechnology 19 (1995), S. 1-10 
    Details
    ISSN: 1573-0778
    Keywords: Namalwa KJM-1 cells ; pro-urokinase ; thrombin resistant ; pro-UKS1 ; stable production ; culture conditions ; serum-free medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Pro-UKS1 was designed as a thrombin-resistant derivative of pro-urokinase (pro-UK) by introducing a glycosylation site using site-directed mutagenesis. An expression plasmid for pro-UKS1, pMo1UKS1SEd1-5, was constructed and introduced into Namalwa KJM-1 cells (Hosoiet al., 1988), and cells resistant to G418 and Methotrexate (MTX) were obtained. Amongst them, the highest pro-UKS1 producer (resistant to 500 nM of MTX), clone 41-8, was selected and further characterized. Clone 41-8 was cultured in serum-free ITPSGF medium (Hosoiet al., 1988). Under the conventional conditions, the concentration of pro-UKS1 reached 26 μg ml−1. Addition of glucose and tri-iodothyronine (T3) improved productivity, and the maximal productivity of pro-UKS1 was 67 μg ml−1 day−1. In this conditioned medium, content of pro-UKS1 was above 80% of total proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749750
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  • 10
    Electronic Resource
    Electronic Resource
    High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium (1989)
    Springer
    Cytotechnology 2 (1989), S. 5-8 
    Details
    ISSN: 1573-0778
    Keywords: hybridoma ; monoclonal antibody ; perfusion culture ; mammalian cell culture ; serum-free culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×107 cells/ml, when the specific perfusion rate was set to 2.3 vol day-1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365409
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