ALBERT

Description: We describe solid-phase cloning (SPC) for high-throughput assembly of expression plasmids. Our method allows PCR products to be put directly into a liquid handler for capture and purification using paramagnetic streptavidin beads and conversion into constructs by subsequent cloning reactions. We present a robust automated protocol for restriction enzyme based SPC and its performance for the cloning of 〉60 000 unique human gene fragments into expression vectors. In addition, we report on SPC-based single-strand assembly for applications where exact control of the sequence between fragments is needed or where multiple inserts are to be assembled. In this approach, the solid support allows for head-to-tail assembly of DNA fragments based on hybridization and polymerase fill-in. The usefulness of head-to-tail SPC was demonstrated by assembly of 〉150 constructs with up to four DNA parts at an average success rate above 80%. We report on several applications for SPC and we suggest it to be particularly suitable for high-throughput efforts using laboratory workstations.

Description: We interpreted the TRIDENT satellite derived gravity field to provide detailed insights into the spatial distribution of the crustal density structures in the area of the Yellow Sea. We used 3-D forward density modelling for the interpretation that incorporated constraints from existing geological and geophysical information. A gravity stripping method is used to separate out the gravity effects of different geological crustal structures. From this analysis we see that (1) the Gunsan sedimentary basin is isostatically compensated. (2) The satellite-derived Bouguer anomalies ranging from 15 to –30 x 10 –5 m s –2 are linked to basin thicknesses in the Yellow Sea. (3) The calculated Moho depth in the Yellow Sea varies from 27 km beneath the deep sedimentary basin to 34 km in the uplifted zones. (4) Moho depth calculations show two distinct areas, characterized by the deepest Moho depths and the largest crustal thicknesses in the Yellow Sea. The one region extends along the Qianliyan Uplift Zone from Jiaodong to Hongsung while the other area extends from southeastern China to Hongsung in the Korean peninsula. Compared to previous works we suggest that they are the part of the collisions zone between North and South China Blocks extending from China to the Korean peninsula via the Yellow Sea.

Description: Regional refinement of the gravity field models from satellite data using spherical radial base functions (SRBF) is an ill-posed problem. This is mainly due to the regional confinement of the data and the base functions, which leads to severe instabilities in the solutions. Here, this ill-posedness as well as the related regularization process are investigated. We compare three methods for the choice of the regularization parameter, which have been frequently used in gravity modelling. These methods are (1) the variance component estimation (VCE), (2) the generalized cross validation (GCV) and (3) the L-curve criterion. A particular emphasis is put on the impact of the SRBF type on the regularization parameter. To do this, we include two types of SRBF which are often used for regional gravity field modelling. These are the Shannon SRBF or the reproducing kernel and the Spline SRBF. The investigations are performed on two months of the real GOCE ultrasensitive gravity gradients over Central Africa and Amazon. The solutions are validated against a state-of-the-art global gravity solution. We conclude that if a proper regularization method is applied, both SRBF deliver more or less the same accuracy. We show that when the Shannon wavelet is used, the L-curve method gives the best results, while with the Spline kernel, the GCV outperforms the other two methods. Moreover, we observe that the estimated coefficients for the Spline kernel cannot be spatially interpreted. In contrast, the coefficients obtained for the Shannon wavelet reflect the energy of the recovered gravity field with a correlation factor of above 95 per cent. Therefore, when combined with the L-curve method, the Shannon SRBF is advantageous for regional gravity field estimation, since it is one of the simplest band-limited SRBF. In addition, it delivers promising solutions and the estimated coefficients represent the characteristics of the gravity field within the target region.

Description: The source parameters and slip distribution of the 2013 May 11 M w 6.1 Minab earthquake are studied using seismology, geodesy and field observations. We observe left-lateral strike-slip motion on a fault striking ENE–WSW; approximately perpendicular to previously studied faults in the Minab–Zendan–Palami fault zone. The fault that ruptured in 2013 is one of a series of ~E–W striking left-lateral faults visible in the geology and geomorphology. These accommodate a velocity field equivalent to right-lateral shear on ~N–S striking planes by clockwise rotations about vertical axes. The presence of these faults can reconcile differences in estimates of fault slip rates in the western Makran from GPS and Quaternary dating. The longitudinal range of shear in the western Makran is likely to be controlled by the distance over which the underthrusting Arabian lithosphere deepens in the transition from continent–continent collision in the Zagros to oceanic subduction in the Makran.

Description: The Earth's outer core is a rotating ellipsoidal shell of compressible, stratified and self-gravitating fluid. As such, in the treatment of geophysical problems a realistic model of this body needs to be considered. In this work, we consider compressible and stratified fluid core models with different stratification parameters, related to the local Brunt-Väisälä frequency, in order to study the effects of the core's density stratification on the frequencies of some of the inertial-gravity modes of this body. The inertial-gravity modes of the core are free oscillations with periods longer than 12 hr. Historically, an incompressible and homogeneous fluid is considered to study these modes and analytical solutions are known for the frequencies and the displacement eigenfunctions of a spherical model. We show that for a compressible and stratified spherical core model the effects of non-neutral density stratification may be significant, and the frequencies of these modes may change from model to model. For example, for a spherical core model the frequency of the spin-over mode, the (2, 1, 1) mode, is unaffected while that of the (4, 1, 1) mode is changed from –0.410 for the Poincaré core model to –0.434, –0.447 and –0.483 for core models with the stability parameter β = –0.001, –0.002 and –0.005, respectively, a maximum change of about 18 per cent when β = –0.005. Our results also show that for small stratification parameter, |β| ≤ 0.005, the frequency of an inertial-gravity mode is a nearly linear function of β but the slope of the line is different for different modes, and that the effects of density stratification on the frequency of a mode is likely related to its spatial structure, which remains the same in different Earth models. We also compute the frequencies of some of the modes of the ‘PREM’ (spherical shell) core model and show that the frequencies of these modes may also be significantly affected by non-zero β.

Description: This paper describes an alternative acceleration approach for determining GRACE monthly gravity field models. The main differences compared to the traditional acceleration approach can be summarized as: (1) The position errors of GRACE orbits in the functional model are taken into account; (2) The range ambiguity is eliminated via the difference of the range measurements and (3) The mean acceleration equation is formed based on Cowell integration. Using this developed approach, a new time-series of GRACE monthly solution spanning the period January 2003 to December 2010, called Tongji_Acc RL01, has been derived. The annual signals from the Tongji_Acc RL01 time-series agree well with those from the GLDAS model. The performance of Tongji_Acc RL01 shows that this new model is comparable with the RL05 models released by CSR and JPL as well as with the RL05a model released by GFZ.

Description: In this paper, we present a method for incorporating prior geological information into potential field data inversion problem. As opposed to the traditional inverse algorithm, our proposed method takes full advantage of prior geological information as a constraint and thus obtains a new objective function for inversion by adding Lagrangian multipliers and slack variables to the traditional inversion method. These additional parameters can be easily solved during iterations. We used both synthetic and observed data sets to test the stability and validity of the proposed method. Our results using synthetic gravity data show that our new method predicts depth and density anomalies more efficiently and accurately than the traditional inversion method that does not include prior geological constraints. Then using observed gravity data in the Three Gorges area and geological constraint information, we obtained the density distribution of the upper and middle crust in this area thus revealing its geological structure. These results confirm the proposed method's validity and indicate its potential application for magnetism data inversion and exploration of geological structures.

Description: Reaching sub-salt hydrocarbon targets in the deeper part of the Gulf of Mexico requires drilling through a salt canopy. The suture zones in the salt canopy are potential drilling hazards due to anomalous pressure behaviour of entrapped sediments. The Pólya vector field of coalescing salt sheets inside the canopy is used to explain suture formation and distinguish between upright and inclined suture contacts. Our analytical models, based on complex potentials, provide exact solutions for multiple source flows as they compete for space when spreading into the viscous continuum of the salt canopy. The velocity gradient tensor yields the strain rate tensor, which is used to map the principal strain rate magnitude inside the canopy. Quantification of one of the principal strain rates is sufficient because the plane deformation assumption ensures the two principal strain rates are equal in magnitude (but of opposite sign); the third principal dimension can have neither strain nor deviatoric stress. Visualization of the locations where the principal stress vanishes or peaks (with highs and lows) is useful for pre-drilling plans because such peaks must be avoided and the stress-free locations provide the safer drilling sites. A case study—of the Walker Ridge region—demonstrates the practical application of our new method.

Description: The Indochina block is important to our understanding of the extrusion model as a consequence of the Indo-Eurasia collision. The lithospheric structure of this block, however, remains obscured due to a lack of sufficient instrumentation for high resolution seismic imaging. We present a shear velocity model derived from Rayleigh wave phase velocity tomography using data from recently deployed seismic networks in this region. Our inversion results for lithospheric structure show strong correlations with tectonic history in this block. A prominent slow-velocity anomaly (5 per cent) is observed in northern Indochina along the Ailao Shan-Red River (ASRR) shear zone including Chuxiong basin, Lanping-Simao fold belt and Thailand rift basin, which has seen extensive deformation events since Eocene. The Khorat Plateau basin is characterized by thick continental keel type lithosphere, consistent with palaeomagnetic and geological observations indicating this basin has experienced much less deformation than the surrounding regions. Additionally, our inversion imaged a sharp, lithospheric-scale velocity contrast across the southeastern segment of ASRR, indicative of a thin and thus relatively weak lithosphere southwest of Red River Fault. The thin lithosphere, low asthenospheric seismic velocities we observe and the average crustal thicknesses in the region suggest that the topography high is dynamically supported by upwelling asthenosphere rather than thickening of the crust/lithosphere. Based on the occurrence of Palaeogene volcanism and its timing, we prefer an explanation of thinning of the lithosphere and allowing a throughgoing fault rather than emplacement of a thin terrane to explain the thin lithosphere. Therefore, the anomalously thin lithosphere between Khorat Plateau and the ASRR in conjunction with other geological observations is generally consistent with the extrusion model for Indochina, which requires localization of lithospheric deformation around tectonic blocks.

Description: On Earth, oceanic plates subduct beneath a variety of overriding plate (OP) styles, from relatively thin and negatively buoyant oceanic OPs to thick and neutrally/positively buoyant continental OPs. The inclusion of an OP in numerical models of self-consistent subduction has been shown to reduce the rate that subducting slabs roll back relative to the equivalent single plate models. We use dynamic, 2-D subduction models to investigate how the mechanical properties, namely viscosity, thickness, and density, of the OP modify the slab rollback rate and the state of stress of the OP. In addition, we examine the role of the subducting plate (SP) viscosity. Because OP deformation accommodates the difference between the slab rollback rate and the far-field OP velocity, we find that the temporal variations in the rollback rate results in temporal variations in OP stress. The slabs in our models roll back rapidly until they reach the lower mantle viscosity increase, at which point the rollback velocity decreases. Concurrent with this reduction in rollback rate is a switch from an OP dominated by extensional stresses to a compressional OP. As in single plate models, the viscosity of the SP exerts a strong control on subducting slab kinematics; weaker slabs exhibit elevated sinking velocities and rollback rates. The SP viscosity also exerts a strong control on the OP stress regime. Weak slabs, either due to reduced bulk viscosity or stress-dependent weakening, have compressional OPs, while strong slabs have dominantly extensional OPs. While varying the viscosity of the OP alone does not substantially affect the OP stress state, we find that the OP thickness and buoyancy plays a substantial role in dictating the rate of slab rollback and OP stress state. Models with thick and/or negatively buoyant OPs have reduced rollback rates, and increased slab dip angles, relative to slabs with thin and/or positively buoyant OPs. Such elevated trench rollback for models with positively buoyant OPs induces extensional stresses in the OP, while OPs that are strongly negatively buoyant are under compression. While rollback is driven by the negative buoyancy of the subducting slab in such models of free subduction, we conclude that the physical properties of the OP potentially play a significant role in modulating both rollback rates and OP deformation style on Earth.

Description: It remains enigmatic how slow slip events (SSEs) interact with other slow seismic events and large distant earthquakes at many subduction zones. Here we model the spatiotemporal slip evolution of the most recent long-term SSE in 2009–2011 in the Bungo Channel region, southwest Japan using GEONET GPS position time-series and a Kalman filter-based, time-dependent slip inversion method. We examine the space-time relationship between the geodetically determined slow slip transient and seismically observed low frequency earthquakes (LFEs) and very-low frequency earthquakes (V-LFEs) near the Nankai trough. We find a strong but distinct temporal correlation between transient slip and LFEs and V-LFEs, suggesting a different relationship to the SSE. We also find the great Tohoku-Oki earthquake appears to disrupt the normal source process of the SSE, probably reflecting large-scale stress redistribution caused by the earthquake. Comparison of the 2009–2011 SSE with others in the same region shows much similarity in slip and moment release, confirming its recurrent nature. Comparison of transient slip with plate coupling shows that slip transients mainly concentrate on the transition zone from strong coupling region to downdip LFEs with transient slip relieving elastic strain accumulation at transitional depth. The less consistent spatial correlation between the long-term SSE and seismic slow earthquakes, and susceptibility of these slow earthquakes to various triggering sources including long-term slow slip, suggests caution in using the seismically determined slow earthquakes as a proxy for slow slip.

Description: Normal mode treatments of the Earth's body tide response were developed in the 1980s to account for the effects of Earth rotation, ellipticity, anelasticity and resonant excitation within the diurnal band. Recent space-geodetic measurements of the Earth's crustal displacement in response to luni-solar tidal forcings have revealed geographical variations that are indicative of aspherical deep mantle structure, thus providing a novel data set for constraining deep mantle elastic and density structure. In light of this, we make use of advances in seismic free oscillation literature to develop a new, generalized normal mode theory for the tidal response within the semi-diurnal and long-period tidal band. Our theory involves a perturbation method that permits an efficient calculation of the impact of aspherical structure on the tidal response. In addition, we introduce a normal mode treatment of anelasticity that is distinct from both earlier work in body tides and the approach adopted in free oscillation seismology. We present several simple numerical applications of the new theory. First, we compute the tidal response of a spherically symmetric, non-rotating, elastic and isotropic Earth model and demonstrate that our predictions match those based on standard Love number theory. Second, we compute perturbations to this response associated with mantle anelasticity and demonstrate that the usual set of seismic modes adopted for this purpose must be augmented by a family of relaxation modes to accurately capture the full effect of anelasticity on the body tide response. Finally, we explore aspherical effects including rotation and we benchmark results from several illustrative case studies of aspherical Earth structure against independent finite-volume numerical calculations of the semi-diurnal body tide response. These tests confirm the accuracy of the normal mode methodology to at least the level of numerical error in the finite-volume predictions. They also demonstrate that full coupling of normal modes, rather than group coupling, is necessary for accurate predictions of the body tide response.

Description: In the central Andes, the Nazca plate displays large along strike variations in dip with a near horizontal subduction angle between 28 and 32°S referred to the Pampean flat slab segment. The upper plate above the Pampean flat slab has high rates of crustal seismicity and active basement cored uplifts. The SIEMBRA experiment, a 43-broad-band-seismic-station array was deployed to better characterize the Pampean flat slab region around 31°S. In this study, we explore the lithospheric structure above the flat slab as a whole and its relation to seismicity. We use the SIEMBRA data to perform a joint inversion of teleseismic receiver functions and Rayleigh wave phase velocity dispersion to constrain the shear wave velocity variations in the lithosphere. Our joint inversion results show: (1) the presence of several upper-plate mid-crustal discontinuities and their lateral extent that are probably related to the terrane accretion history; (2) zones of high shear wave velocity in the upper-plate lower crust associated with a weak Moho signal consistent with the hypothesis of partial eclogitization in the lower crust; (3) the presence of low shear-wave velocities at ~100 km depth interpreted as the subducting oceanic crust. Finally, in order to investigate the relation of the lithospheric structure to seismicity, we determine an optimal velocity–depth model based on the joint inversion results and use it to perform regional moment tensor inversions (SMTI) of crustal and slab earthquakes. The SMTI for 18 earthquakes that occurred between 2007 and 2009 in the flat slab region below Argentina, indicates systematically shallower focal depths for slab earthquakes (compared with inversions using previous velocity models). This suggests that the slab seismicity is concentrated mostly between 90 and 110 km depths within the subducting Nazca plate's oceanic crust and likely related to dehydration. In addition, the slab earthquakes exhibit extensional focal mechanisms suggesting new faulting at the edges of the flat portion of the slab. SMTI solutions for upper-plate crustal earthquakes match well the geological observations of reactivated structures and agree with crustal shortening. Our new constraints on flat slab structure can aid earthquake characterization for regional seismic hazard assessment and efforts to help understand the mechanisms for slab flattening in the central Andes.

Description: Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is the directed integration of chemically synthesized single-stranded deoxyribonucleic acid (oligonucleotides) into the chromosome of Escherichia coli during replication. Herein, we present a general co-selection strategy in multiplex genome engineering that yields highly modified cells. We demonstrate that disparate sites throughout the genome can be easily modified simultaneously by leveraging selectable markers within 500 kb of the target sites. We apply this technique to the modification of 80 sites in the E. coli genome.

Description: The elastic displacement and stress fields due to rectangular faults and opening-mode fractures within an anisotropic homogeneous half-space are derived in this paper. The solution is expressed in terms of the mathematically elegant and computationally powerful Stroh formalism and can be applied to the generally anisotropic half-space or a transversely isotropic half-space with any oriented isotropic plane. For any flat fault or opening-mode fracture of polygonal shape, one needs only to carry out a simple line integral from 0 to in order to express the fault-induced response. Numerical examples are presented to demonstrate the effect of the anisotropy and fault orientation on the internal and surface responses of the half-space. Our results prove that both rock anisotropy and fault orientation could dramatically change the fields in the domain and one needs to consider these properties as accurately as possible to be able to predict the response in the domain precisely. Anisotropy of the rock mass may alter the dominant displacement and stress components at observation points in the model domain as compared to the isotropic case.

Description: Shear deformation of partially molten rock in laboratory experiments causes the emergence of melt-enriched sheets (bands in cross-section) that are aligned at about 15°–20° to the shear plane. Deformation and deviatoric stress also cause the coherent alignment of pores at the grain scale. This leads to a melt-preferred orientation that may, in turn, give rise to an anisotropic permeability. Here we develop a simple, general model of anisotropic permeability in partially molten rocks. We use linearized analysis and nonlinear numerical solutions to investigate its behaviour under simple-shear deformation. In particular, we consider implications of the model for the emergence and angle of melt-rich bands. Anisotropic permeability affects the angle of bands and, in a certain parameter regime, it can give rise to low angles consistent with experiments. However, the conditions required for this regime have a narrow range and seem unlikely to be entirely met by experiments. Anisotropic permeability may nonetheless affect melt transport and the behaviour of partially molten rocks in Earth's mantle.

Description: Eastern Anatolia region between north–south colliding Arabian and Eurasian plates has no significant crustal root and shallow (upper) mantle flow beneath seems to be vertically supporting its high topography. It has a high surface heat flow and the underlying mantle is characterized by low seismic velocity zones. Using a mantle density/temperature variation field derived from P -wave seismic velocity, current shallow mantle flow and resultant dynamic topography of Eastern Anatolia and adjacent Arabian foreland and Caucasus areas were calculated along a vertical section. The section crosses the tectonic boundaries interrelated with slab bodies (high seismic velocity/cold regions) and the low velocity zones above the slabs. According to the modelling experiments, the surface topography of Eastern Anatolia seems to be supported by shallow mantle flow dynamics. On the other hand, residual topography for the region was calculated using high resolution crustal thickness data. Positive residual topography that suggests an undercompensated state of Eastern Anatolia is in concordance with the dynamic topography anomaly. The modelled local shallow mantle flow support due to the density contrast between hot (low velocity) zones and underlying cold slab bodies beneath the area may be the present-day snapshot of the mantle flow uplift in Eastern Anatolia presence of which was previously suggested.

Description: Crystallization experiments in the dendritic regime have been carried out in hypergravity conditions (from 1 to 1300 g) from an ammonium chloride solution (NH 4 Cl and H 2 O). A commercial centrifuge was equipped with a slip ring so that electric power (needed for a Peltier device and a heating element), temperature and ultrasonic signals could be transmitted between the experimental setup and the laboratory. Ultrasound measurements (2–6 MHz) were used to detect the position of the front of the mushy zone and to determine attenuation in the mush. Temperature measurements were used to control a Peltier element extracting heat from the bottom of the setup and to monitor the evolution of crystallization in the mush and in the liquid. A significant increase of solid fraction and attenuation in the mush is observed as gravity is increased. Kinetic undercooling is significant in our experiments and has been included in a macroscopic mush model. The other ingredients of the model are conservation of energy and chemical species, along with heat/species transfer between the mush and the liquid phase: boundary-layer exchanges at the top of the mush and bulk convection within the mush (formation of chimneys). The outputs of the model compare well with our experiments. We have then run the model in a range of parameters suitable for the Earth's inner core. This has shown the role of bulk mush convection for the inner core and the reason why a solid fraction very close to unity should be expected. We have also run melting experiments: after crystallization of a mush, the liquid has been heated from above until the mush started to melt, while the bottom cold temperature was maintained. These melting experiments were motivated by the possible local melting at the inner core boundary that has been invoked to explain the formation of the anomalously slow F-layer at the bottom of the outer core or inner core hemispherical asymmetry. Oddly, the consequences of melting are an increase in solid fraction and a decrease in attenuation. It is hence possible that surface seismic velocity and attenuation of the inner core are strongly affected by melting.

Description: The coupling that exists between surface processes and deformation within both the shallow crust and the deeper mantle-lithosphere has stimulated the development of computational geodynamic models that incorporate a free surface boundary condition. We introduce a treatment of this boundary condition that is suitable for staggered grid, finite difference schemes employing a structured Eulerian mesh. Our interface capturing treatment discretizes the free surface boundary condition via an interface that conforms with the edges of control volumes (e.g. a ‘staircase’ representation) and requires only local stencil modifications to be performed. Comparisons with analytic solutions verify that the method is first-order accurate. Additional intermodel comparisons are performed between known reference models to further validate our free surface approximation. Lastly, we demonstrate the applicability of a multigrid solver to our free surface methodology and demonstrate that the local stencil modifications do not strongly influence the convergence of the iterative solver.

Description: Observations of the temporal variations in the volume flux of a plume can provide useful constraints on geodynamic models of plumes and plume-plate interactions. Furthermore, they can be compared with observations at other plumes and may be analysed further to understand the nature and cause of the variations. The volume plume flux is typically derived from a sum of edifice and compensation root volumes. The former can be obtained via the application of regional–residual separation procedures that split the observed relief into regional (swell) and residual (edifice) components, while the latter is generally inferred from the former using local (Airy) or regional (flexural) compensation models. Most regional–residual techniques used in past studies give non-unique results and provide no estimates of the uncertainty in the separation, which impacts the significance of the results. Here, the optimal robust separator (ORS) method achieves a unique separation for the swell and edifice components of the Hawaiian Ridge and furthermore obtain confidence bounds on the total volume flux. A fast spectral method for plate flexure with different edifice and infill densities is used to determine compensation volumes. Although my flux estimates have assigned confidence bounds, these are much smaller than the flux estimates themselves. A comparison of my new results to published volume flux curves shows that my revised flux estimates are lower by a factor of 2–3. Reproducing the prior higher results demonstrates that these discrepancies appear to be related to shortcomings in the implementation of the methodology used in the separation. The variability in the Hawaiian plume flux occurs at two different time scales: A short (1–2 Myr) periodicity related to the spacing of islands and seamounts, which ultimately is related to plume-plate flexural interactions, and a much longer (10–15 Myr) periodicity that may be related to plate kinematic changes. Superimposed on these trends may be an exponential increase towards more recent times, but this trend may also be explained by a higher flux level during the period when the plume was positioned beneath the relatively younger lithospheric segment bracketed by the Murray and Molokai fracture zones. Landslides and erosion of the edifice may imply an underestimation of total volumes by 5–10 per cent. The main uncertainty facing studies of plume flux is related to the unknown quantity of magmatic underplating.

Description: Ellesmere Island in Arctic Canada displays a complex geological evolution. The region was affected by two distinct orogenies, the Palaeozoic Ellesmerian orogeny (the Caledonian equivalent in Arctic Canada and Northern Greenland) and the Palaeogene Eurekan orogeny, related to the opening of Baffin Bay and the consequent convergence of the Greenland plate. The details of this complex evolution and the present-day deep structure are poorly constrained in this remote area and deep geophysical data are sparse. Receiver function analysis of seven temporary broad-band seismometers of the Ellesmere Island Lithosphere Experiment complemented by two permanent stations provides important data on the crustal velocity structure of Ellesmere Island. The crustal expression of the northernmost tectonic block of Ellesmere Island (~82°–83°N), Pearya, which was accreted during the Ellesmerian orogeny, is similar to that at the southernmost part, which is part of the Precambrian Laurentian (North America-Greenland) craton. Both segments have thick crystalline crust (~35–36 km) and comparable velocity–depth profiles. In contrast, crustal thickness in central Ellesmere Island decreases from ~24–30 km in the Eurekan fold and thrust belt (~79.7°–80.6°N) to ~16–20 km in the Hazen Stable Block (HSB; ~80.6°–81.4°N) and is covered by a thick succession of metasediments. A deep crustal root (~48 km) at ~79.6°N is interpreted as cratonic crust flexed beneath the Eurekan fold and thrust belt. The Carboniferous to Palaeogene sedimentary succession of the Sverdrup Basin is inferred to be up to 1–4 km thick, comparable to geologically-based estimates, near the western margin of the HSB.

Description: Joint analysis of the seismic velocities and geoid, gravity and gravity gradients are used to constrain the viscosity profile within the mantle as well as the lateral density variations. Recent ESA's Gravity field and steady-state Ocean Circulation Explorer measurements of the second-order derivatives of the Earth's gravity potential give new possibilities to determine these mantle properties. Using a simple mantle model and seismic tomography results, we investigate how the gravitational potential, the three components of the gravity vector and the gravity gradients can bring information on the radial viscosity profile and on the mantle mass anomalies. We start with lateral density variations in the Earth's mantle based either on slab history or deduced from seismic tomography. The main uncertainties are: for the latter case, the relationship between seismic velocity and density—the so-called density/velocity scaling factor—and for the former case, the variation with depth of the density contrast between the cold slabs and the surrounding mantle. We perform a Monte Carlo search for the viscosity and the density/velocity scaling factor profiles within the mantle, which allows to fit the observed geoid, gravity and gradients of gravity. We compute the posterior probability distribution of the unknown parameters, and find that the gravity gradients improve the estimate of the scaling factor within the upper mantle, because of their sensitivity to the masses within the upper mantle, whereas the geoid and the gravity better constrain the scaling factor in the lower mantle. In the upper mantle, it is less than 0.02 in the upper part and about 0.08–0.14 in the lower part, and it is significantly larger for depths greater than 1200 km (about 0.32–0.34). In any case, the density/velocity scaling factor between 670 and 1150 km depth is not well constrained. We show that the viscosity of the upper part of the mantle is strongly correlated with the viscosity of the lower part of the mantle and that the viscosity profile is characterized by a decrease in the lower part of the upper mantle (about 10 20 –2  x  10 20 Pa s) and by an increase (about 10 23 –2  x  10 23 Pa s) at the top of the lower mantle (between 670 and 1150 km). The viscosity of the mantle below 1150 km depth is well estimated in our Monte Carlo search and is about 10 22 –4  x  10 22 Pa s.

Description: A sequence of large earthquakes occurred along the North Anatolian fault in the 20th century. These earthquakes, including the 1999 Izmit/Düzce earthquakes, generally propagated westward towards the Marmara Sea, defining the Main Marmara fault as a potential seismic gap. It is important to conduct a detailed assessment of the seismic hazards along the main Marmara fault because the megacity Istanbul lies only approximately 10 km north of the eastern segment of the Main Marmara fault, which is referred to as the Princes’ Islands Fault segment (PIF). Here, we study the locking status of this fault segment to evaluate the seismic hazard potential. For the first time, combined ascending and descending Interferometric Synthetic Aperture Radar and Global Positioning System observations were used to investigate the crustal deformation associated with the PIF. After careful corrections of the estimated ground velocity, a deformation pattern relating to fault locking near the Princes’ Islands was identified. The modeling results revealed that the slip rate and locking depth of the fault segment show a clear trade-off, which were estimated as 18.9 ± 7.2 mm yr –1 and 12.1 ± 7.0 km, respectively. With a moment accumulation rate of 1.7 ± 0.4  x  10 17 Nm yr –1 (proportional to the product of slip rate and locking depth), our results imply a build-up of a geodetic moment on the PIF and therefore a potential for earthquake hazards in the vicinity of the Istanbul megacity.

Description: Microfluidics may revolutionize our ability to write synthetic DNA by addressing several fundamental limitations associated with generating novel genetic constructs. Here we report the first de novo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reaction droplets using programmable digital microfluidics. Specifically, we developed Programmable Order Polymerization (POP), Microfluidic Combinatorial Assembly of DNA (M-CAD) and Microfluidic In-vitro Cloning (MIC) and applied them to de novo synthesis, combinatorial assembly and cell-free cloning of genes, respectively. Proof-of-concept for these methods was demonstrated by programming an autonomous microfluidic system to construct and clone libraries of yeast ribosome binding sites and bacterial Azurine, which were then retrieved in individual droplets and validated. The ability to rapidly and robustly generate designer DNA molecules in an autonomous manner should have wide application in biological research and development.

Description: Recent developments in synthetic biology have positioned lactic acid bacteria (LAB) as a major class of cellular chassis for applications. To achieve the full potential of LAB, one fundamental prerequisite is the capacity for rapid engineering of complex gene networks, such as natural biosynthetic pathways and multicomponent synthetic circuits, into which cellular functions are encoded. Here, we present a synthetic biology platform for rapid construction and optimization of large-scale gene networks in LAB. The platform involves a copy-controlled shuttle for hosting target networks and two associated strategies that enable efficient genetic editing and phenotypic validation. By using a nisin biosynthesis pathway and its variants as examples, we demonstrated multiplex, continuous editing of small DNA parts, such as ribosome-binding sites, as well as efficient manipulation of large building blocks such as genes and operons. To showcase the platform, we applied it to expand the phenotypic diversity of the nisin pathway by quickly generating a library of 63 pathway variants. We further demonstrated its utility by altering the regulatory topology of the nisin pathway for constitutive bacteriocin biosynthesis. This work demonstrates the feasibility of rapid and advanced engineering of gene networks in LAB, fostering their applications in biomedicine and other areas.

Description: Large-scale topography may be due to several causes, including (1) variations in crustal thickness and density structure, (2) oceanic lithosphere age differences, (3) subcrustal density variations in the continental lithosphere and (4) convective flow in the mantle beneath the lithosphere. The last contribution in particular may change with time and be responsible for continental inundations; distinguishing between these contributions is therefore important for linking Earth's history to its observed geological record. As a step towards this goal, this paper aims at such distinction for the present-day topography: the approach taken is deriving a ‘model’ topography due to contributions (3) and (4), along with a model geoid, using a geodynamic mantle flow model. Both lithosphere thickness and density anomalies beneath the lithosphere are inferred from seismic tomography. Density anomalies within the continental lithosphere are uncertain, because they are probably due to variations in composition and temperature, making a simple scaling from seismic to density anomalies inappropriate. Therefore, we test a number of different assumptions regarding these. As a reality check, model topography is compared, in terms of both correlation and amplitude ratio, to ‘residual’ topography, which follows from observed topography after subtracting contributions (1) and (2). The model geoid is compared to observations as well. Comparatively good agreement is found if there is either an excess density of 0.2 per cent in the lithosphere above 150 km depth, with anomalies below as inferred from tomography, or if the excess density is 0.4 per cent in the entire lithosphere. Further, a good fit is found for viscosity 10 20 Pa s in the asthenosphere, increasing to 10 23 Pa s in the lower mantle above D'. Results are quite dependent on which tomography models they are based on; for some recent ones, topography correlation is 0.6, many smaller scale features are matched, topography amplitude is less than 30 per cent too large, while geoid variance reduction exceeds 70 per cent—overall a considerable improvement compared to previous models. Correlation becomes less if smaller scale features (corresponding to spherical harmonic degrees 15 and higher), which are probably largely due to anomalies in the lithosphere, are also considered. Comparison of results with different viscosity structures, and a regional comparison of amplitude ratios, indicates that lateral viscosity variations can be quite strong, but only leading to moderate variations in model topography of a factor probably less than two.

Description: We present a distributed slip model for the 1999 M w 6.3 Chamoli earthquake of north India using interferometric synthetic aperture radar (InSAR) data from both ascending and descending orbits and Bayesian estimation of confidence levels and trade-offs of the model geometry parameters. The results of fault-slip inversion in an elastic half-space show that the earthquake ruptured a $9 _{ - 2.2}^{\circ + 3.4}$ northeast-dipping plane with a maximum slip of ~1 m. The fault plane is located at a depth of ~ $15.9_{ - 3.0}^{ + 1.1}$ km and is ~120 km north of the Main Frontal Thrust, implying that the rupture plane was on the northernmost detachment near the mid-crustal ramp of the Main Himalayan Thrust. The InSAR-determined moment is 3.35 x 10 18 Nm with a shear modulus of 30 GPa, equivalent to M w 6.3, which is smaller than the seismic moment estimates of M w 6.4–6.6. Possible reasons for this discrepancy include the trade-off between moment and depth, uncertainties in seismic moment tensor components for shallow dip-slip earthquakes and the role of earth structure models in the inversions. The released seismic energy from recent earthquakes in the Garhwal region is far less than the accumulated strain energy since the 1803 M s 7.5 earthquake, implying substantial hazard of future great earthquakes.

Description: We use a Bayesian formalism combined with a grid node discretization for the linear inversion of gravimetric data in terms of 3-D density distribution. The forward modelling and the inversion method are derived from seismological inversion techniques in order to facilitate joint inversion or interpretation of density and seismic velocity models. The Bayesian formulation introduces covariance matrices on model parameters to regularize the ill-posed problem and reduce the non-uniqueness of the solution. This formalism favours smooth solutions and allows us to specify a spatial correlation length and to perform inversions at multiple scales. We also extract resolution parameters from the resolution matrix to discuss how well our density models are resolved. This method is applied to the inversion of data from the volcanic island of Basse-Terre in Guadeloupe, Lesser Antilles. A series of synthetic tests are performed to investigate advantages and limitations of the methodology in this context. This study results in the first 3-D density models of the island of Basse-Terre for which we identify: (i) a southward decrease of densities parallel to the migration of volcanic activity within the island, (ii) three dense anomalies beneath Petite Plaine Valley, Beaugendre Valley and the Grande-Découverte-Carmichaël-Soufrière Complex that may reflect the trace of former major volcanic feeding systems, (iii) shallow low-density anomalies in the southern part of Basse-Terre, especially around La Soufrière active volcano, Piton de Bouillante edifice and along the western coast, reflecting the presence of hydrothermal systems and fractured and altered rocks.

Description: Oblique convergence across Tibet leads to slip partitioning with the coexistence of strike-slip, normal and thrust motion on major fault systems. A key point is to understand and model how faults interact and accumulate strain at depth. Here, we extract ground deformation across the Haiyuan Fault restraining bend, at the northeastern boundary of the Tibetan plateau, from Envisat radar data spanning the 2001–2011 period. We show that the complexity of the surface displacement field can be explained by the partitioning of a uniform deep-seated convergence. Mountains and sand dunes in the study area make the radar data processing challenging and require the latest developments in processing procedures for Synthetic Aperture Radar interferometry. The processing strategy is based on a small baseline approach. Before unwrapping, we correct for atmospheric phase delays from global atmospheric models and digital elevation model errors. A series of filtering steps is applied to improve the signal-to-noise ratio across high ranges of the Tibetan plateau and the phase unwrapping capability across the fault, required for reliable estimate of fault movement. We then jointly invert our InSAR time-series together with published GPS displacements to test a proposed long-term slip-partitioning model between the Haiyuan and Gulang left-lateral Faults and the Qilian Shan thrusts. We explore the geometry of the fault system at depth and associated slip rates using a Bayesian approach and test the consistency of present-day geodetic surface displacements with a long-term tectonic model. We determine a uniform convergence rate of 10 [8.6–11.5] mm yr –1 with an N89 [81–97]°E across the whole fault system, with a variable partitioning west and east of a major extensional fault-jog (the Tianzhu pull-apart basin). Our 2-D model of two profiles perpendicular to the fault system gives a quantitative understanding of how crustal deformation is accommodated by the various branches of this thrust/strike-slip fault system and demonstrates how the geometry of the Haiyuan fault system controls the partitioning of the deep secular motion.

Description: In this paper, we study stability of the rate, state and temperature friction (RSTF) model. The Segall and Rice approach is used to model heat transfer at the sliding interface with its surroundings. The effect of pore pressure is not considered in the model to avoid the complex expression for critical stiffness. Linear stability analysis of the spring-mass sliding system is carried out with the ageing law under the quasistatic conditions in order to determine the critical stiffness above which sliding behaviour changes from unstable to stable or vice versa. Our numerical simulations establish that critical stiffness of the heated surface may increase or decrease from corresponding to the critical stiffness of the unheated surface depending on the relative values of two contradictory parameters related with velocity effect and temperature effect. Parametric studies are also carried out to understand shear velocity and temperature of the sliding surface dependence of steady friction. The RSTF model is also used to study the gravity induced failure of a creeping rock slope and the results are justified.

Description: We compute the gravimetric factor at the Chandler wobble (CW) frequency using time-series from superconducting gravimeters (SG) longer than a decade. We first individually process the polar motion and data at each individual gravity station to estimate the gravimetric factor amplitude and phase, then we make a global analysis by applying a stacking method to different subsets of up to seven SG stations. The stacking is an efficient way of getting rid of local effects and improving the signal-to-noise ratio of the combined data sets. Using the stacking method, we find a gravimetric factor amplitude and phase of 1.118 ± 0.016 and –0.45 ± 0.66 deg, respectively, which is smaller in amplitude than expected. The sources of error are then carefully considered. For both local and global analyses, the uncertainties on our results are reliably constrained by computing the standard deviation of the estimates of the gravimetric factor amplitude and phase for increasing length of the time-series. Constraints on the CW anelastic dissipation can be set since any departure of the gravimetric factor from its elastic value may provide some insights into the dissipative processes that occur at the CW period. In particular, assuming given rheological models for the Earth's mantle enables us to make the link between the gravimetric factor phase and the CW quality factor.

Description: Analysing independent 1-yr data sets of 10 European superconducting gravimeters (SG) reveals statistically significant temporal variations of M2 tidal parameters. Both common short-term (〈2 yr) and long-term (〉2 yr) features are identified in all SG time-series but one. The averaged variations of the amplitude factor are about 0.2. The path of load vector variations equivalent to the temporal changes of tidal parameters suggests the presence of an 8.85 yr modulation (lunar perigee). The tidal waves having the potential to modulate M2 with this period belong to the 3rd degree constituents. Their amplitude factors turn out to be much closer to body tide model predictions than that of the main 2nd degree M2, which indicates ocean loading for 3rd degree waves to be less prominent than for 2nd degree waves within the M2 group. These two different responses to the loading suggest that the observed modulation is more due to insufficient frequency resolution of limited time-series rather than to time variable loading. Presently, SG gravity time-series are still too short to prove if time variable loading processes are involved too as in case of the annual M2 modulation known to appear for analysis intervals of less than 1 yr. Whatever the variations are caused by, they provide the upper accuracy limit for earth model validation and permit estimating the temporal stability of SG scale factors and assessing the quality of gravity time-series.

Description: A sequence of large earthquakes occurred along the North Anatolian fault in the 20th century. These earthquakes, including the 1999 Izmit/Düzce earthquakes, generally propagated westward towards the Marmara Sea, defining the Main Marmara fault as a potential seismic gap. It is important to conduct a detailed assessment of the seismic hazards along the main Marmara fault because the megacity Istanbul lies only approximately 10 km north of the eastern segment of the Main Marmara fault, which is referred to as the Princes’ Islands Fault segment (PIF). Here, we study the locking status of this fault segment to evaluate the seismic hazard potential. For the first time, combined ascending and descending Interferometric Synthetic Aperture Radar and Global Positioning System observations were used to investigate the crustal deformation associated with the PIF. After careful corrections of the estimated ground velocity, a deformation pattern relating to fault locking near the Princes’ Islands was identified. The modeling results revealed that the slip rate and locking depth of the fault segment show a clear trade-off, which were estimated as 18.9 ± 7.2 mm yr –1 and 12.1 ± 7.0 km, respectively. With a moment accumulation rate of 1.7 ± 0.4  x  10 17 Nm yr –1 (proportional to the product of slip rate and locking depth), our results imply a build-up of a geodetic moment on the PIF and therefore a potential for earthquake hazards in the vicinity of the Istanbul megacity.

Description: Deformation analysis in general and strain analysis in particular using permanent GPS networks require proper analysis of time-series in which all functional effects are taken into consideration and all stochastic effects are captured using an appropriate noise model. This contribution addresses both issues when considering the strain parameters of a GPS network. Estimates of spatial correlation, time correlated noise, and multivariate power spectrum for daily position time-series of the Southern California Integrated GPS Network (SCIGN) stations collected between 1996 and 2011 are obtained. Significant signals with periods of 13.63 d and those related to the GPS draconitic year are identified in these time-series. We aim to assess the effect of a realistic noise model of the series on the uncertainties of the strain parameters including displacements, normal and shear strains, and rotations. For the SCIGN network considered, the following results are highlighted. Contrary to the common belief, the uncertainties of the displacements parameters become smaller when taking a realistic noise model into account. This however was not the case when assessing the noise characteristics of the normal and shear strain, and rotation parameters. The uncertainties increase nearly by a factor of two, in agreement to what is expected. Some of the significant deformation parameters of the white noise model become less significant in case of the realistic noise model.

Description: The Siple Coast region of Antarctica contains a number of fast-flowing ice streams, which control the dynamics and mass balance of the region. These ice streams are known to undergo stagnation and reactivation cycles, which lead to ice thickness changes that may be sufficient to excite a viscous solid Earth response (glacial isostatic adjustment; GIA). This study aims to quantify Siple Coast ice thickness changes during the last 2000 yr in order to determine the degree to which they might contribute to GIA and associated present-day bedrock uplift rates. This is important because accurate modelling of GIA is necessary to determine the rate of present-day ice-mass change from satellite gravimetry. Recently-published reconstructions of ice-stream variability were used to create a suite of kinematic models for the stagnation-related thickening of Kamb Ice Stream since ~1850 AD, and a GIA model was used to predict present-day deformation rates in response to this thickening. A number of longer-term loading scenarios, which include the stagnation and reactivation of ice streams across the Siple Coast over the past 2000 yr, were also constructed, and used to investigate the longer term GIA signal in the region. Uplift rates for each of the ice loading histories, based on a range of earth models, were compared with regional GPS-observed uplift rates and an empirical GIA estimate. We estimate Kamb Ice Stream to have thickened by 70–130 m since stagnation ~165 years ago. Modelled present-day vertical motion in response to this load increase peaks at –17 mm yr –1 (i.e. 17 mm yr –1 subsidence) for the weakest earth models tested here. Comparison of the solid Earth response to ice load changes throughout the last glacial cycle, including ice stream stagnation and reactivation across the Siple Coast during the last 2000 yr, with an empirical GIA estimate suggests that the upper mantle viscosity of the region is greater than 1 x 10 20 Pa s. When upper mantle viscosity values of 1 x 10 20  Pa s or smaller are combined with our suite of ice-load scenarios we predict uplift rates across Siple Coast that are at least 4 mm yr –1 smaller than those predicted by the empirical GIA estimate. GPS data are unable to further constrain model parameters due to the distance of the GPS sites from the study area. Our results demonstrate that Late Holocene ice load changes related to the stagnation and reactivation of ice streams on the Siple Coast may play a dominant role in defining the present-day uplift signal. However, both the detailed Earth structure and deglacial history of the region need to be better constrained in order to reduce uncertainties associated with the GIA signal of this region.

Description: The Castrovillari scarps (Cfs) are located in northern Calabria (Italy) and consist of three main WSW-dipping fault scarps resulting from multiple rupture events. At the surface, these scarps are defined by multiple breaks in slope. Despite its near-surface complexity, the faults likely merge to form a single normal fault at about 200 m depth, which we refer to as the Castrovillari fault. We present the results of a multidisciplinary and multiscale study at a selected site of the Cfs with the aim to (i) characterize the geometry at the surface and at depth and (ii) obtain constraints on the fault slip history. We investigate the site by merging data from quantitative geomorphological analyses, electrical resistivity and ground penetrating radar surveys, and palaeoseismological trenching along a ~40 m high scarp. The closely spaced investigations allow us to reconstruct the shallow stratigraphy, define the fault locations, and measure the faulted stratigraphic offsets down to 20 m depth. Despite the varying resolutions, each of the adopted approaches suggests the presence of sub-parallel fault planes below the scarps at approximately the same location. The merged datasets permit the evaluation of the fault array (along strike for 220 m within a 370-m-wide zone). The main fault zone consists of two closely spaced NW–SE striking fault planes in the upper portion of the scarp slope and another fault at the scarp foot. The 3-D image of the fault surfaces shows west to southwest dipping planes with values between 70° and 80°; the two closely spaced planes join at about 200 m below the surface. The 8-to-12-m-high upper fault, which shows the higher vertical displacements, accommodated most of the deformation during the Holocene. Results from the trenching analysis indicate a minimum slip per event of 0.6 m and a maximum short-term slip rate of 0.6 mm yr –1 for the Cf. The shallow subsurface imaging techniques are particularly helpful in evaluating the possible field uncertainties related to postfaulting modification by erosional/depositional/human processes, such as within stream valleys and urbanized zones.

Description: Optimizing bio-production involves strain and process improvements performed as discrete steps. However, environment impacts genotype and a strain that is optimal under one set of conditions may not be under different conditions. We present a methodology to simultaneously vary genetic and process factors, so that both can be guided by design of experiments (DOE). Advances in DNA assembly and gene insulation facilitate this approach by accelerating multi-gene pathway construction and the statistical interpretation of screening data. This is applied to a 6-aminocaproic acid (6-ACA) pathway in Escherichia coli consisting of six heterologous enzymes. A 32-member fraction factorial library is designed that simultaneously perturbs expression and media composition. This is compared to a 64-member full factorial library just varying expression (0.64 Mb of DNA assembly). Statistical analysis of the screening data from these libraries leads to different predictions as to whether the expression of enzymes needs to increase or decrease. Therefore, if genotype and media were varied separately this would lead to a suboptimal combination. This is applied to the design of a strain and media composition that increases 6-ACA from 9 to 48 mg/l in a single optimization step. This work introduces a generalizable platform to co-optimize genetic and non-genetic factors.

Description: Crustal vertical deformation (CVD) observed by continuous GPS height time-series can be explained largely by surface loading effects recovered from both Gravity Recover and Climate Experiment (GRACE) and General Circulation Models (GCMs) data. We first show that lower degree CVD spatial spectrum due to the Earth's elastic response to a uniform surface loading plays more important roles than that of high-degree case. We then demonstrate that GRACE data with 300–400 km spatial resolution have the ability to detect 99 per cent power of global and regional CVD in spatial spectrum domain using a global frequency–wavenumber spectrum method. We can just use either GRACE or GCMs 36 degree/order (d/o) spherical harmonic coefficients (SHCs) which correspond to 500 km spatial resolution to acquire more than 90 per cent variance of total CVD modeled by up to 180 d/o SHCs at 98 per cent global gridpoints. Globally, CVD modeled by GRACE loading can explain 72 per cent annual amplitude and 69 per cent variance of GPS observed height time-series, which is better than the GCMs results of 64 per cent for annual amplitude and 41 per cent for variance. Using a three cornered hat method, we also show that the noise level of monthly averaged CVD is about 3 mm for both GPS height time-series and GRACE loading result, while that of GCMs result is only 1.3 mm.

Description: With the progress of mantle convection modelling over the last decade, it now becomes possible to solve for the dynamics of the interior flow and the surface tectonics to first order. We show here that tectonic data (like surface kinematics and seafloor age distribution) and mantle convection models with plate-like behaviour can in principle be combined to reconstruct mantle convection. We present a sequential data assimilation method, based on suboptimal schemes derived from the Kalman filter, where surface velocities and seafloor age maps are not used as boundary conditions for the flow, but as data to assimilate. Two stages (a forecast followed by an analysis) are repeated sequentially to take into account data observed at different times. Whenever observations are available, an analysis infers the most probable state of the mantle at this time, considering a prior guess (supplied by the forecast) and the new observations at hand, using the classical best linear unbiased estimate. Between two observation times, the evolution of the mantle is governed by the forward model of mantle convection. This method is applied to synthetic 2-D spherical annulus mantle cases to evaluate its efficiency. We compare the reference evolutions to the estimations obtained by data assimilation. Two parameters control the behaviour of the scheme: the time between two analyses, and the amplitude of noise in the synthetic observations. Our technique proves to be efficient in retrieving temperature field evolutions provided the time between two analyses is 10 Myr. If the amplitude of the a priori error on the observations is large (30 per cent), our method provides a better estimate of surface tectonics than the observations, taking advantage of the information within the physics of convection.

Description: In this study, the Moho depth is estimated based on the refined spherical Bouguer gravity disturbance and DTM2006 topographic data using the Vening Meinesz-Moritz gravimetric-isostatic hypothesis. In this context, we compute the refined spherical Bouguer gravity disturbances in a set of 1° x 1° blocks. The spherical terrain correction, a residual correction to each Bouguer shell, is computed using rock heights and ice sheet thicknesses from the DTM2006 and Earth2014 models. The study illustrates that the defined simple Bouguer gravity disturbance corrected for the density variations of the oceans, ice sheets and sediment basins and also the non-isostatic effects needs a significant terrain correction to become the refined Bouguer gravity disturbance, and that the isostatic gravity disturbance is significantly better defined by the latter disturbance plus a compensation attraction. Our study shows that despite the fact that the lateral variation of the crustal depth is rather smooth, the terrain affects the result most significantly in many areas. The global numerical results show that the estimated Moho depths by the simple and refined spherical Bouguer gravity disturbances and the seismic CRUST1.0 model agree to 5.6 and 2.7 km in RMS, respectively. Also, the mean value differences are 1.7 and 0.2 km, respectively. Two regional numerical studies show that the RMS differences between the Moho depths estimated based on the simple and refined spherical Bouguer gravity disturbance and that using CRUST1.0 model yield fits of 4.9 and 3.2 km in South America and yield 3.2 and 3.4 km in Fennoscandia, respectively.

Description: We present a new method to derive 3-D surface deformation from an integration of interferometric synthetic aperture radar (InSAR) images and Global Navigation Satellite System (GNSS) observations based on Akaike's Bayesian Information Criterion (ABIC), considering relationship between deformations of neighbouring locations. This method avoids interpolated errors by excluding the interpolation of GNSS into the same spatial resolution as InSAR images and harnesses the data sets and the prior smooth constraints of surface deformation objectively and simultaneously by using ABIC, which were inherently unresolved in previous studies. In particular, we define surface roughness measuring smoothing degree to evaluate the performance of the prior constraints and deduce the formula of the covariance for the estimation errors to estimate the uncertainty of modelled solution. We validate this method using synthetic tests and the 2008 M w 7.9 Wenchuan earthquake. We find that the optimal weights associated with ABIC minimum are generally at trade-off locations that balance contributions from InSAR, GNSS data sets and the prior constraints. We use this method to evaluate the influence of the interpolated errors from the Ordinary Kriging algorithm on the derivation of surface deformation. Tests show that the interpolated errors may contribute to biasing very large weights imposed on Kriged GNSS data, suggesting that fixing the relative weights is required in this case. We also make a comparison with SISTEM method, indicating that our method allows obtaining better estimations even with sparse GNSS observations. In addition, this method can be generalized to provide a solution for situations where some types of data sets are lacking and can be exploited further to account for data sets such as the integration of displacements along radar lines and offsets along satellite tracks.

Description: The rifting episode that occurred in Dabbahu–Manda–Hararo (Ethiopia) between 2005 and 2010 during which 14 dyke intrusions were emitted, was a unique opportunity to study interactions between tectonic deformation and magmatic processes. While magmatism has been shown to control primarily the spatial and temporal distribution of dyke intrusions during this accretion sequence, the role of faults in accommodating plate spreading in rift segments is poorly understood. During interdyking periods, transient ground deformation due to magma movement is generally observed. Investigating such a small-scale deformation and in particular the movement along faults during these periods will help understanding the factors that trigger fault movement in magmatic rifts. We analyse fault activity during three interdyking periods: 2006 December–June (d0–d1), 2007 January–July (d5–d6) and 2009 November–January (d10–d11). The time–space evolution of surface displacements along ~700 faults is derived from pairs of ascending and descending SAR interferograms. Surface slip distributions are then compared with codyking ground deformation fields. The results show that faults are mainly activated above the areas affected by magma emplacement during interdyking periods. A detailed analysis of brittle deformation during the six months following the 2005 September intrusion shows asymmetric deformation on the rift shoulders, with significant opening on faults located to the west of the dyke. We explain this feature by the activation of westward dipping pre-existing faults, with block rotations in between. In addition, we observe that the strip encompassing the activated faults narrows by 30 per cent from co- to interdyking period. This suggests that magma keeps migrating to shallower depths after the dyke intrusion. During a rifting episode, activation of faults in a pre-existing fracture network therefore seems to be mainly controlled by deep magma processes.

Description: While it has been known for some time that offsets in the time-series of Global Navigation Satellite System (GNSS) position estimates degrade station velocity determinations, the magnitude of the effect has not been clear. Using products of the International GNSS Service (IGS), we assess the impact empirically by injecting progressively larger numbers of artificial offsets and solving for a series of long-term secular GNSS frames. Our results show that the stability of the IGS global frame datum is fairly robust, with significant effects at the formal error level only for the R x (and Y-pole) and R z rotational orientations. On the other hand, station velocity estimates are more seriously affected, especially the vertical component. For the typical IGS station, the mean vertical rate uncertainty is already limited to 0.34 mm yr –1 for the current set of position discontinuities. If the number of breaks doubles, which might occur using newer detection schemes, then that uncertainty will worsen by ~40 per cent to 0.48 mm yr –1 . This error source is generally a more important component of realistic velocity uncertainties than any other, including accounting for temporal correlations in the GNSS data. The only way to improve future GNSS velocity estimates is to severely limit manmade displacements at the tracking stations.

Description: We investigate how uncertainties in seismic and density structure of the crust propagate to uncertainties in mantle density structure. The analysis is based on interpretation of residual upper-mantle gravity anomalies which are calculated by subtracting (stripping) the gravitational effect of the crust from the observed satellite gravity field data (GOCE Direct release 3). Thus calculated residual mantle gravity anomalies are caused mainly by a heterogeneous density distribution in the upper mantle. Given a relatively small range of expected compositional density variations in the lithospheric mantle, knowledge on uncertainties associated with incomplete information on crustal structure is of utmost importance for progress in gravity modelling. Uncertainties in the residual upper-mantle gravity anomalies result chiefly from uncertainties in (i) seismic V P velocity–density conversion for the crust and (ii) uncertainties in the seismic crustal structure (thickness and average V P velocities of individual crustal layers, including the sedimentary cover). We examine the propagation of these uncertainties into determinations of lithospheric mantle density and analyse both sources of possible uncertainties by applying different velocity-to-density conversions and by introducing variations into the crustal structure which correspond to typical resolution of high-quality and low-quality seismic models. We apply our analysis to Siberia (the West Siberian Basin and the Siberian Craton) for which a new regional seismic crustal model, SibCrust, has recently become available. For the same region, we also compute upper-mantle gravity and density anomalies based on three global crustal models (CRUST 5.1, CRUST 2.0 and CRUST 1.0) and compare the results based on four different crustal models. A large uncertainty in the V P -to-density conversion may result in the uncertainty in lithospheric mantle density anomalies of ca. 0.02–0.03 g cm –3 (i.e. 0.5–1 per cent, which is comparable to compositional density anomalies expected for continental lithosphere mantle). Similar values of uncertainties may be caused by a 0.2 km s –1 error in average crustal V P velocities or by a 2 km uncertainty in the Moho depth. One of the largest uncertainties is caused by errors in thickness of the sedimentary layer, and a 2 km error leads to ca. 0.03 g cm –3 error in lithospheric mantle densities. Large deviations (locally ±10 km) of the Moho depth in global crustal models (CRUST 5.1, CRUST2.0 and CRUST1.0) from the high-resolution regional seismic model of the crust, SibCrust, may produce artefact residual mantle gravity anomalies of up to ±150 mGal locally, caused by large errors in crustal gravity corrections. These errors in gravity anomalies produce up to ca. 0.04 g cm –3 ( ca. 1.2 per cent) errors in density of the lithospheric mantle, which may well correspond to the amplitude of real density anomalies in the mantle. Our results demonstrate that gravity modelling alone cannot reliably constrain the crustal structure, including the Moho depth and thickness of sediments.

Description: We have extended backwards from 2001 to 1979 the current release 05 (RL05) of the Gravity Recovery and Climate Experiment (GRACE) Atmospheric and Oceanic De-aliasing Level-1B (AOD1B) product and studied the impact of this and a previous release 04 (RL04) of the AOD1B product on precise orbits of five altimetry satellites (ERS-1, ERS-2, TOPEX/Poseidon, Envisat and Jason-1) for the time span 1991–2012, as compared to the case when no AOD1B product is used. We have found that using AOD1B RL05 product reduces root mean square (RMS) fits of satellite laser ranging (SLR) observations by about 1.0–6.4 per cent, 2-d arc overlaps in radial, cross-track and along-track directions by about 1.3–12.0, 0.3–10.0 and 2.0–10.0 per cent, respectively, for various satellites tested, as compared to the case without AOD1B product. Using AOD1B RL05 product instead of RL04 one reduces SLR RMS fits by 0.1–0.7 per cent, 2-d arc overlaps in radial, cross-track and along-track directions by 0.1–0.6, 0.1–1.3 and 0.2–1.2 per cent, respectively, for the satellite orbits tested. The multi-mission crossover analysis shows that the application of an AOD1B product reduces the scatter of radial errors by 0.4–2.8 per cent for the satellite missions studied. At the regions with the most pronounced changes the use of the AOD1B products improves the consistency between the sea level as measured by the TOPEX and ERS-2 missions and by the Jason-1 and Envisat missions by 5 to 10 per cent (globally by about 2 per cent). The results of our study show that extended AOD1B RL05 product performs better than the AOD1B RL04 and improves orbits of altimetry satellites and consistency of sea level products.

Description: A chemistry-based artificial restriction DNA cutter (ARCUT) was recently prepared from Ce(IV)/EDTA complex and a pair of pseudo-complementary peptide nucleic acids. This cutter has freely tunable scission-site and site specificity. In this article, homologous recombination (HR) in human cells was promoted by cutting a substrate DNA with ARCUT, and the efficiency of this bioprocess was optimized by various chemical and biological approaches. Of two kinds of terminal structure formed by ARCUT, 3'-overhang termini provided by 1.7-fold higher efficiency than 5'-overhang termini. A longer homology length (e.g. 698 bp) was about 2-fold more favorable than shorter one (e.g. 100 bp). When the cell cycle was synchronized to G2/M phase with nocodazole, the HR was promoted by about 2-fold. Repression of the NHEJ-relevant proteins Ku70 and Ku80 by siRNA increased the efficiency by 2- to 3-fold. It was indicated that appropriate combination of all these chemical and biological approaches should be very effective to promote ARCUT-mediated HR in human cells.

Description: We describe a novel cloning method termed SLiCE (Seamless L i gation Cloning Extract) that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into recombinant DNA molecules in a single in vitro recombination reaction. SLiCE overcomes the sequence limitations of traditional cloning methods, facilitates seamless cloning by recombining short end homologies (≥15 bp) with or without flanking heterologous sequences and provides an effective strategy for directional subcloning of DNA fragments from Bacteria Artificial Chromosomes (BACs) or other sources. SLiCE is highly cost effective as a number of standard laboratory bacterial strains can serve as sources for SLiCE extract. In addition, the cloning efficiencies and capabilities of these strains can be greatly improved by simple genetic modifications. As an example, we modified the DH10B Escherichia coli strain to express an optimized prophage Red recombination system. This strain, termed PPY, facilitates SLiCE with very high efficiencies and demonstrates the versatility of the method.

Description: Targeted gene addition to mammalian genomes is central to biotechnology, basic research and gene therapy. For example, gene targeting to the ROSA26 locus by homologous recombination in embryonic stem cells is commonly used for mouse transgenesis to achieve ubiquitous and persistent transgene expression. However, conventional methods are not readily adaptable to gene targeting in other cell types. The emerging zinc finger nuclease (ZFN) technology facilitates gene targeting in diverse species and cell types, but an optimal strategy for engineering highly active ZFNs is still unclear. We used a modular assembly approach to build ZFNs that target the ROSA26 locus. ZFN activity was dependent on the number of modules in each zinc finger array. The ZFNs were active in a variety of cell types in a time- and dose-dependent manner. The ZFNs directed gene addition to the ROSA26 locus, which enhanced the level of sustained gene expression, the uniformity of gene expression within clonal cell populations and the reproducibility of gene expression between clones. These ZFNs are a promising resource for cell engineering, mouse transgenesis and pre-clinical gene therapy studies. Furthermore, this characterization of the modular assembly method provides general insights into the implementation of the ZFN technology.

Description: A simple approach for creating libraries of circularly permuted proteins is described that is called PERMutation Using Transposase Engineering (PERMUTE). In PERMUTE, the transposase MuA is used to randomly insert a minitransposon that can function as a protein expression vector into a plasmid that contains the open reading frame (ORF) being permuted. A library of vectors that express different permuted variants of the ORF-encoded protein is created by: (i) using bacteria to select for target vectors that acquire an integrated minitransposon; (ii) excising the ensemble of ORFs that contain an integrated minitransposon from the selected vectors; and (iii) circularizing the ensemble of ORFs containing integrated minitransposons using intramolecular ligation. Construction of a Thermotoga neapolitana adenylate kinase (AK) library using PERMUTE revealed that this approach produces vectors that express circularly permuted proteins with distinct sequence diversity from existing methods. In addition, selection of this library for variants that complement the growth of Escherichia coli with a temperature-sensitive AK identified functional proteins with novel architectures, suggesting that PERMUTE will be useful for the directed evolution of proteins with new functions.

Description: Synthetic scaffolds that permit spatial and temporal organization of enzymes in living cells are a promising post-translational strategy for controlling the flow of information in both metabolic and signaling pathways. Here, we describe the use of plasmid DNA as a stable, robust and configurable scaffold for arranging biosynthetic enzymes in the cytoplasm of Escherichia coli . This involved conversion of individual enzymes into custom DNA-binding proteins by genetic fusion to zinc-finger domains that specifically bind unique DNA sequences. When expressed in cells that carried a rationally designed DNA scaffold comprising corresponding zinc finger binding sites, the titers of diverse metabolic products, including resveratrol, 1,2-propanediol and mevalonate were increased as a function of the scaffold architecture. These results highlight the utility of DNA scaffolds for assembling biosynthetic enzymes into functional metabolic structures. Beyond metabolism, we anticipate that DNA scaffolds may be useful in sequestering different types of enzymes for specifying the output of biological signaling pathways or for coordinating other assembly-line processes such as protein folding, degradation and post-translational modifications.

Description: To reveal the full potential of human pluripotent stem cells, new methods for rapid, site-specific genomic engineering are needed. Here, we describe a system for precise genetic modification of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). We identified a novel human locus, H11 , located in a safe, intergenic, transcriptionally active region of chromosome 22, as the recipient site, to provide robust, ubiquitous expression of inserted genes. Recipient cell lines were established by site-specific placement of a ‘landing pad’ cassette carrying attP sites for phiC31 and Bxb1 integrases at the H11 locus by spontaneous or TALEN-assisted homologous recombination. Dual integrase cassette exchange (DICE) mediated by phiC31 and Bxb1 integrases was used to insert genes of interest flanked by phiC31 and Bxb1 attB sites at the H11 locus, replacing the landing pad. This system provided complete control over content, direction and copy number of inserted genes, with a specificity of 100%. A series of genes, including mCherry and various combinations of the neural transcription factors LMX1a, FOXA2 and OTX2, were inserted in recipient cell lines derived from H9 ESC, as well as iPSC lines derived from a Parkinson’s disease patient and a normal sibling control. The DICE system offers rapid, efficient and precise gene insertion in ESC and iPSC and is particularly well suited for repeated modifications of the same locus.

Description: Recombineering, which is the use of homologous recombination for DNA engineering in Escherichia coli , usually uses antibiotic selection to identify the intended recombinant. When combined in a second step with counterselection using a small molecule toxin, seamless products can be obtained. Here, we report the advantages of a genetic strategy using CcdB as the counterselectable agent. Expression of CcdB is toxic to E. coli in the absence of the CcdA antidote so counterselection is initiated by the removal of CcdA expression. CcdB counterselection is robust and does not require titrations or experiment-to-experiment optimization. Because counterselection strategies necessarily differ according to the copy number of the target, we describe two variations. For multi-copy targets, we use two E. coli hosts so that counterselection is exerted by the transformation step that is needed to separate the recombined and unrecombined plasmids. For single copy targets, we put the ccdA gene onto the temperature-sensitive pSC101 Red expression plasmid so that counterselection is exerted by the standard temperature shift to remove the expression plasmid. To reduce unwanted intramolecular recombination, we also combined CcdB counterselection with Redα omission. These options improve the use of counterselection in recombineering with BACs, plasmids and the E. coli chromosome.

Description: The two-step process of selection and counter-selection is a standard way to enable genetic modification and engineering of bacterial genomes using homologous recombination methods. The tetA and sacB genes are contained in a DNA cassette and confer a novel dual counter-selection system. Expression of tetA confers bacterial resistance to tetracycline (Tc R ) and also causes sensitivity to the lipophillic chelator fusaric acid; sacB causes sensitivity to sucrose. These two genes are introduced as a joint DNA cassette into Escherichia coli by selection for Tc R . A medium containing both fusaric acid and sucrose has been developed, in which, coexpression of tetA-sacB is orders of magnitude more sensitive as a counter-selection agent than either gene alone. In conjunction with the homologous recombination methods of recombineering and P1 transduction, this powerful system has been used to select changes in the bacterial genome that cannot be directly detected by other counter-selection systems.

Description: DNA ‘assembly’ from ‘building blocks’ remains a cornerstone in synthetic biology, whether it be for gene synthesis (~1 kb), pathway engineering (~10 kb) or synthetic genomes (〉100 kb). Despite numerous advances in the techniques used for DNA assembly, verification of the assembly is still a necessity, which becomes cost-prohibitive and a logistical challenge with increasing scale. Here we describe for the first time a comprehensive, high-throughput solution for structural DNA assembly verification by restriction digest using exhaustive in silico enzyme screening, rolling circle amplification of plasmid DNA, capillary electrophoresis and automated digest pattern recognition. This low-cost and robust methodology has been successfully used to screen over 31 000 clones of DNA constructs at 〈$1 per sample.

Description: Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid procedure for DNA fragment assembly using site-specific recombination by C31 integrase. Using six orthogonal attP / attB recombination site pairs with different overlap sequences, we can assemble up to five DNA fragments in a defined order and insert them into a plasmid vector in a single recombination reaction. C31 integrase-mediated assembly is highly efficient, allowing production of large libraries suitable for combinatorial gene assembly strategies. The resultant assemblies contain arrays of DNA cassettes separated by recombination sites, which can be used to manipulate the assembly by further recombination. We illustrate the utility of these procedures to (i) assemble functional metabolic pathways containing three, four or five genes; (ii) optimize productivity of two model metabolic pathways by combinatorial assembly with randomization of gene order or ribosome binding site strength; and (iii) modify an assembled metabolic pathway by gene replacement or addition.

Description: A conditional gene expression system that is fast-acting, is tunable and achieves single-gene specificity was recently developed for yeast. A gene placed directly downstream of a modified GAL1 promoter containing six Zif268 binding sequences (with single nucleotide spacing) was shown to be selectively inducible in the presence of β-estradiol, so long as cells express the artificial transcription factor, Z 3 EV (a fusion of the Zif268 DNA binding domain, the ligand binding domain of the human estrogen receptor and viral protein 16). We show the strength of Z 3 EV-responsive promoters can be modified using straightforward design principles. By moving Zif268 binding sites toward the transcription start site, expression output can be nearly doubled. Despite the reported requirement of estrogen receptor dimerization for hormone-dependent activation, a single binding site suffices for target gene activation. Target gene expression levels correlate with promoter binding site copy number and we engineer a set of inducible promoter chassis with different input–output characteristics. Finally, the coupling between inducer identity and gene activation is flexible: the ligand specificity of Z 3 EV can be re-programmed to respond to a non-hormone small molecule with only five amino acid substitutions in the human estrogen receptor domain, which may prove useful for industrial applications.

Description: We demonstrate a system for cloning and modifying the chloroplast genome from the green alga, Chlamydomonas reinhardtii . Through extensive use of sequence stabilization strategies, the ex vivo genome is assembled in yeast from a collection of overlapping fragments. The assembled genome is then moved into bacteria for large-scale preparations and transformed into C. reinhardtii cells. This system also allows for the generation of simultaneous, systematic and complex genetic modifications at multiple loci in vivo. We use this system to substitute genes encoding core subunits of the photosynthetic apparatus with orthologs from a related alga, Scenedesmus obliquus . Once transformed into algae, the substituted genome recombines with the endogenous genome, resulting in a hybrid plastome comprising modifications in disparate loci. The in vivo function of the genomes described herein demonstrates that simultaneous engineering of multiple sites within the chloroplast genome is now possible. This work represents the first steps toward a novel approach for creating genetic diversity in any or all regions of a chloroplast genome.

Description: The increasing interest in genetic manipulation of bacterial host metabolic pathways for protein or small molecule production has led to a need to add new genes to a chromosome quickly and easily without leaving behind a selectable marker. The present report describes a vector and four-day procedure that enable site-specific chromosomal insertion of cloned genes in a context insulated from external transcription, usable once in a construction series. The use of rhamnose-inducible transcription from rhaBp allows regulation of the inserted genes independently of the commonly used IPTG and arabinose strategies. Using lacZ as a reporter, we first show that expression from the rhamnose promoter is tightly regulatable, exhibiting very low leakage of background expression compared with background, and moderate rhamnose-induced expression compared with IPTG-induced expression from lacp . Second, the expression of a DNA methyltransferase was used to show that rhamnose regulation yielded on-off expression of this enzyme, such that a resident high-copy plasmid was either fully sensitive or fully resistant to isoschizomer restriction enzyme cleavage. In both cases, growth medium manipulation allows intermediate levels of expression. The vehicle can also be adapted as an ORF-cloning vector.

Description: The development of economical and high-throughput gene synthesis technology has been hampered by the high occurrence of errors in the synthesized products, which requires expensive labor and time to correct. Here, we describe an error correction reaction (ECR), which employs Surveyor, a mismatch-specific DNA endonuclease, to remove errors from synthetic genes. In ECR reactions, errors are revealed as mismatches by re-annealing of the synthetic gene products. Mismatches are recognized and excised by a combination of mismatch-specific endonuclease and 3'-〉5' exonuclease activities in the reaction mixture. Finally, overlap extension polymerase chain reaction (OE-PCR) re-assembles the resulting fragments into intact genes. The process can be iterated for increased fidelity. With two iterations, we were able to reduce errors in synthetic genes by 〉16-fold, yielding a final error rate of ~1 in 8700 bp.

Description: For a period of about 1 yr between the summers of 2010 and 2011, 25 broad-band seismographs were deployed in a roughly linear array across the eastern end of the Qaidam basin and the Qilian Shan in the northeastern Tibetan plateau. This region is probably the most suitable place to study the ongoing convergence interaction between the high Tibetan plateau and the main Asian continental plate. Low-frequency P receiver function analysis of the data provides an image of the crust and mantle down to 700 km depth. In addition to the Moho at 45–65 km depth beneath the profile, the 410 and 660 km discontinuities bounding the mantle transition zone can be identified at 400–410 and 650–660 km depths, respectively. A possible increase in temperature in the upper mantle thought to exist beneath the northern part of the high Tibetan plateau is thus confined to this part of the plateau and lower upper-mantle temperatures similar to those beneath southern Tibet occur beneath the Qaidam basin and Qilian Shan. When higher frequencies are included in the P receiver function analysis, a positive Ps converter dipping down to the south from 70–75 km depth at 37.9°N to about 110 km depth at 36°N is imaged. As this feature is only seen in high-frequency images and not in the low-frequency image, it is modelled as the positive Ps conversion from the base of an approximately 5-km-thick anisotropic layer at the top of the Asian mantle lithosphere which is currently subducting. This south-dipping converter continues to the south on the INDEPTH IV profile. S receiver function analysis completes the image of the structure below the Qilian Shan profile with the identification of the lithosphere–asthenosphere boundary (LAB). The LAB of the Asian Plate is identified for a reference slowness of 6.4 s deg –1 at 12–14 s (105–125 km depth) between 38 and 41°N below the northern part of the S receiver function profile. To the south it increases in depth such that it is at about 19 s (170 km depth) between 34 and 35°N at the southern end of the profile. The LAB of the Asian Plate occurs at similar depths on the INDEPTH IV profile at the latitudes where the INDEPTH IV and Qilian Shan profiles overlap. As on the INDEPTH IV profile to the south, between 34 and 35°N at the southern end of the Qilian Shan profile there is evidence from the S receiver functions for the LAB of a separate Tibetan Plate.

Description: The paper in question by Van Camp and co-authors [MVC] challenges previous work showing that ground gravity data arising from hydrology can provide a consistent signal for the comparison with satellite gravity data. The data sets used are similar to those used previously, that is, the gravity field as measured by the GRACE satellites versus ground-based data from superconducting gravimeters (SGs) over the same continental area, in this case Central Europe. One of the main impediments in this paper is the presentation that is frequently confusing and misleading as to what the data analysis really shows, for example, the irregular treatment of annual components that are first subtracted then reappear in the analysis. More importantly, we disagree on specific points. Two calculations are included in our comment to illustrate where we believe that the processing in [MVC] paper is deficient. The first deals with their erroneous treatment of the global hydrology using a truncated spherical harmonic approach which explains almost a factor 2 error in their computation of the loading. The second shows the effect of making the wrong assumption in the GRACE/hydrology/surface gravity comparison by inverting the whole of the hydrology loading for underground stations. We also challenge their claims that empirical orthogonal function techniques cannot be done in the presence of periodic components, and that SG data cannot be corrected for comparisons with GRACE data. The main conclusion of their paper, that there is little coherence between ground gravity stations and this invalidates GRACE comparisons, is therefore questionable. There is nothing in [MVC] that contradicts any of the previous papers that have shown clearly a strong relation between seasonal signals obtained from both ground gravity and GRACE satellite data.

Description: The influence of changes in surface ice-mass redistribution and associated viscoelastic response of the Earth, known as glacial isostatic adjustment (GIA), on the Earth's rotational dynamics has long been known. Equally important is the effect of the changes in the rotational dynamics on the viscoelastic deformation of the Earth. This signal, known as the rotational feedback, or more precisely, the rotational feedback on the sea level equation, has been mathematically described by the sea level equation extended for the term that is proportional to perturbation in the centrifugal potential and the second-degree tidal Love number. The perturbation in the centrifugal force due to changes in the Earth's rotational dynamics enters not only into the sea level equation, but also into the conservation law of linear momentum such that the internal viscoelastic force, the perturbation in the gravitational force and the perturbation in the centrifugal force are in balance. Adding the centrifugal-force perturbation to the linear-momentum balance creates an additional rotational feedback on the viscoelastic deformations of the Earth. We term this feedback mechanism, which is studied in this paper, as the rotational feedback on the linear-momentum balance. We extend both the time-domain method for modelling the GIA response of laterally heterogeneous earth models developed by Martinec and the traditional Laplace-domain method for modelling the GIA-induced rotational response to surface loading by considering the rotational feedback on linear-momentum balance. The correctness of the mathematical extensions of the methods is validated numerically by comparing the polar-motion response to the GIA process and the rotationally induced degree 2 and order 1 spherical harmonic component of the surface vertical displacement and gravity field. We present the difference between the case where the rotational feedback on linear-momentum balance is considered against that where it is not. Numerical simulations show that the resulting difference in radial displacement and sea level change between these situations since the Last Glacial Maximum reaches values of ±25 and ±1.8 m, respectively. Furthermore, the surface deformation pattern is modified by up to 10 per cent in areas of former or ongoing glaciation, but by up to 50 per cent at the bottom of the southern Indian ocean. This also results in the movement of coastlines during the last deglaciation to differ between the two cases due to the difference in the ocean loading, which is seen for instance in the area around Hudson Bay, Canada and along the Chinese, Australian or Argentinian coastlines.

Description: Lower and upper bounds for present deformation rates across faults in central California between the San Andreas Fault and Pacific coast are estimated from a new Global Positioning System (GPS) velocity field for central, western California in light of geodetic evidence presented in a companion paper for slow, but significant deformation within the Pacific Plate between young seafloor in the eastern Pacific and older seafloor elsewhere on the plate. Transects of the GPS velocity field across the San Andreas Fault between Parkfield and San Juan Buatista, where fault slip is dominated by creep and the velocity field thus reveals the off-fault deformation, show that GPS sites in westernmost California move approximately parallel to the fault at an average rate of 3.4 ± 0.4 mm yr –1 relative to the older interior of the Pacific Plate, but only 1.8 ± 0.6 mm yr –1 if the Pacific Plate frame of reference is corrected for deformation within the plate. Modelled interseismic elastic deformation from the weakly coupled creeping segment of the San Andreas Fault is an order-of-magnitude too small to explain the southeastward motions of coastal sites in western California. Similarly, models that maximize residual viscoelastic deformation from the 1857 Fort Tejon and 1906 San Francisco earthquakes mismatch both the rates and directions of GPS site motions in central California relative to the Pacific Plate. Neither thus explains the site motions southwest of the San Andreas fault, indicating that the site motions measure deformation across faults and folds outboard of the San Andreas Fault. The non-zero site velocities thus constitute strong evidence for active folding and faulting outboard from the creeping segment of the San Andreas Fault and suggest limits of 0–2 mm yr –1 for the Rinconada Fault slip rate and 1.8 ± 0.6 to 3.4 ± 0.4 mm yr –1 for the slip rates integrated across near-coastal faults such as the Hosgri, San Gregorio and San Simeon faults.

Description: We combine new, well-determined GPS velocities from Clarion, Guadalupe and Socorro islands on young seafloor in the eastern Pacific basin with newly estimated velocities for 26 GPS sites from older seafloor in the central, western and southern parts of the Pacific Plate to test for deformation within the interior of the Pacific Plate and estimate the viscosity of the asthenosphere below the plate. Relative to a Pacific Plate reference frame defined from the velocities of the 26 GPS sites in other areas of the Pacific Plate, GPS sites on Clarion and Guadalupe islands in the eastern Pacific move 1.2 ± 0.6 mm yr –1 (1) towards S09°W ± 38° and 1.9 ± 0.3 mm yr –1 towards S19°E ± 10°, respectively. The two velocities, which are consistent within their 95 per cent uncertainties, both differ significantly from Pacific Plate motion. Transient volcanic deformation related to a 1993–1996 eruption of the Socorro Island shield volcano renders our GPS velocity from that island unreliable for the tectonic analysis although its motion is also southward like those of Clarion and Guadalupe islands. We test but reject the possibilities that drift of Earth's origin in ITRF2008 or unmodelled elastic offsets due to large-magnitude earthquakes around the Pacific rim since 1993 can be invoked to explain the apparent slow southward motions of Clarion and Guadalupe islands. Similarly, corrections to the Pacific Plate GPS velocity field for possible viscoelastic deformation triggered by large-magnitude earthquakes since 1950 also fail to explain the southward motions of the two islands. Viscoelastic models with prescribed asthenospheric viscosities lower than 1  x 10 19 Pa s instead introduce statistically significant inconsistencies into the Pacific Plate velocity field, suggesting that the viscosity of the asthenosphere below the plate is higher than 1  x 10 19 Pa s. Elastic deformation from locked Pacific–North America Plate boundary faults is also too small to explain the southward motions of the two islands. Horizontal thermal contraction of the plate interior may explain the motion observed at Clarion and Guadalupe islands, as might long-term tectonic deformation of the plate interior.

Description: In this study, a new method for computing the sensitivity of the glacial isostatic adjustment (GIA) forward solution with respect to the Earth's mantle viscosity, the so-called the forward sensitivity method (FSM), and a method for computing the gradient of data misfit with respect to viscosity parameters, the so-called adjoint-state method (ASM), are presented. These advanced formal methods complement each other in the inverse modelling of GIA-related observations. When solving this inverse problem, the first step is to calculate the forward sensitivities by the FSM and use them to fix the model parameters that do not affect the forward model solution, as well as identifying and removing redundant parts of the inferred viscosity structure. Once the viscosity model is optimized in view of the forward sensitivities, the minimization of the data misfit with respect to the viscosity parameters can be carried out by a gradient technique which makes use of the ASM. The aim is this paper is to derive the FSM and ASM in the forms that are closely associated with the forward solver of GIA developed by Martinec. Since this method is based on a continuous form of the forward model equations, which are then discretized by spectral and finite elements, we first derive the continuous forms of the FSM and ASM and then discretize them by the spectral and finite elements used in the discretization of the forward model equations. The advantage of this approach is that all three methods (forward, FSM and ASM) have the same matrix of equations and use the same methodology for the implementation of the time evolution of stresses. The only difference between the forward method and the FSM and ASM is that the different numerical differencing schemes for the time evolution of the Maxwell and generalized Maxwell viscous stresses are applied in the respective methods. However, it requires only a little extra computational time for carrying out the FSM and ASM numerically. An straightforward approach to compute the gradient of the data misfit is the brute-force method, whereby the partial derivatives of the misfit with respect to model parameters are approximated by the centred difference of two forward model runs. Although the brute-force method is useful for computing the gradient of the data misfit with respect to a small number of model parameters, it becomes expensive for a viscosity model with a large number of parameters. The ASM offers an efficient alternative for computing the gradient of the misfit since the computational time of the ASM is independent of the number of viscosity parameters. The ASM is thus highly efficient for calculating the gradient of the misfit for models with large numbers of parameters. However, the forward-model solution for each time step must be stored, hence the memory demands scale linearly with the number of time steps. This is the main drawback of the ASM.

Description: During megathrust earthquakes, great ruptures are accompanied by large scale mass redistribution inside the solid Earth and by ocean mass redistribution due to bathymetry changes. These large scale mass displacements can be detected using the monthly gravity maps of the GRACE satellite mission. In recent years it has become increasingly common to use the long wavelength changes in the Earth's gravity field observed by GRACE to infer seismic source properties for large megathrust earthquakes. An important advantage of space gravimetry is that it is independent from the availability of land for its measurements. This is relevant for observation of megathrust earthquakes, which occur mostly offshore, such as the $M_{\text{w}} \sim 9$ 2004 Sumatra–Andaman, 2010 Maule (Chile) and 2011 Tohoku-Oki (Japan) events. In Broerse et al. , we examined the effect of the presence of an ocean above the rupture on long wavelength gravity changes and showed it to be of the first order. Here we revisit the implementation of an ocean layer through the sea level equation and compare the results with approximated methods that have been used in the literature. One of the simplifications usually lies in the assumption of a globally uniform ocean layer. We show that especially in the case of the 2010 Maule earthquake, due to the closeness of the South American continent, the uniform ocean assumption is not valid and causes errors up to 57 per cent for modelled peak geoid height changes (expressed at a spherical harmonic truncation degree of 40). In addition, we show that when a large amount of slip occurs close to the trench, horizontal motions of the ocean floor play a mayor role in the ocean contribution to gravity changes. Using a slip model of the 2011 Tohoku-Oki earthquake that places the majority of slip close to the surface, the peak value in geoid height change increases by 50 per cent due to horizontal ocean floor motion. Furthermore, we test the influence of the maximum spherical harmonic degree at which the sea level equation is performed for sea level changes occurring along coastlines, which shows to be important for relative sea level changes occurring along the shore. Finally, we demonstrate that ocean floor loading, self-gravitation of water and conservation of water mass are of second order importance for coseismic gravity changes. When GRACE observations are used to determine earthquake parameters such as seismic moment or source depth, the uniform ocean layer method introduces large biases, depending on the location of the rupture with respect to the continent. The same holds for interpreting shallow slip when horizontal motions are not properly accounted for in the ocean contribution. In both cases the depth at which slip occurs will be underestimated.

Description: The precise control of gene expression is essential in basic biological research as well as in biotechnological applications. Most regulated systems available in yeast enable only the overexpression of the target gene, excluding the possibility of intermediate or weak expression. Moreover, these systems are frequently toxic or depend on growth conditions. We constructed a heterologous transcription factor that overcomes these limitations. Our system is a fusion of the bacterial LexA DNA-binding protein, the human estrogen receptor (ER) and an activation domain (AD). The activity of this chimera, called LexA-ER-AD, is tightly regulated by the hormone β-estradiol. The selection of the AD proved to be crucial to avoid toxic effects and to define the range of activity that can be precisely tuned with β-estradiol. As our system is based on a heterologous DNA-binding domain, induction in different metabolic contexts is possible. Additionally, by controlling the number of LexA-binding sites in the target promoter, one can scale the expression levels up or down. Overall, our LexA-ER-AD system is a valuable tool to precisely control gene expression in different experimental contexts without toxic side effects.

Description: Inspired by the developments of synthetic biology and the need for improved genetic tools to exploit cyanobacteria for the production of renewable bioproducts, we developed a versatile platform for the construction of broad-host-range vector systems. This platform includes the following features: (i) an efficient assembly strategy in which modules released from 3 to 4 donor plasmids or produced by polymerase chain reaction are assembled by isothermal assembly guided by short GC-rich overlap sequences. (ii) A growing library of molecular devices categorized in three major groups: (a) replication and chromosomal integration; (b) antibiotic resistance; (c) functional modules. These modules can be assembled in different combinations to construct a variety of autonomously replicating plasmids and suicide plasmids for gene knockout and knockin. (iii) A web service, the CYANO-VECTOR assembly portal, which was built to organize the various modules, facilitate the in silico construction of plasmids, and encourage the use of this system. This work also resulted in the construction of an improved broad-host-range replicon derived from RSF1010, which replicates in several phylogenetically distinct strains including a new experimental model strain Synechocystis sp. WHSyn, and the characterization of nine antibiotic cassettes, four reporter genes, four promoters, and a ribozyme-based insulator in several diverse cyanobacterial strains.

Description: Relative to the gravitational potential energy of the Earth's monopole, the multipole energy has received far less attention. In this paper, we recapitulate the basic physics from first principles and derive the formulas for multipole energies in analogy to classical electrostatic theory. We focus on the zonal quadrupole energy associated with the Earth's oblateness, the dominant term in Earth's gravity field apart from the monopole. We find the gravitational energy E oblateness 10 –6 | E monopole | = +2.5 x 10 26 J. We examine the implications of E oblateness and its changes associated with long-term ‘secular’ decreases in the oblateness parameter J 2 . We find the rate of loss of E oblateness due to the Earth rounding induced by the present-day GIA is about –200 GW, an amount quite significant in the kinetic energy budget of the mantle heat engine that drives the plate tectonics that has been estimated to be ~1 TW. We also assert that the tidal braking and the global earthquake dislocations, both resulting in Earth rounding on long-term geological timescales, are accompanied with a secular decrease of E oblateness at nearly the same rate of several GW.

Description: Long-term volcanic subsidence provides insight into intereruptive processes, which comprise the longest portion of the eruptive cycle. Ground-based geodetic surveys of Medicine Lake Volcano (MLV), northern CA, document subsidence at rates of ~–10 mm yr –1 between 1954 and 2004. The long observation period plus the duration and stable magnitude of this signal presents an ideal opportunity to study long-term volcanic deformation, but this first requires accurate knowledge of the geometry and magnitude of the source. Best-fitting analytical source models to past levelling and GPS data sets show conflicting source parameters—primarily the model depth. To overcome this, we combine multiple tracks of InSAR data, each with a different look angle, to improve upon the spatial resolution of ground-based measurements. We compare the results from InSAR to those of past geodetic studies, extending the geodetic record to 2011 and demonstrating that subsidence at MLV continues at ~–10 mm yr –1 . Using geophysical inversions, we obtain the best-fitting analytical source model—a sill located at 9–10 km depth beneath the caldera. This model geometry is similar to those of past studies, providing a good fit to the high spatial density of InSAR measurements, while accounting for the high ratio of vertical to horizontal deformation derived from InSAR and recorded by existing levelling and GPS data sets. We discuss possible causes of subsidence and show that this model supports the hypothesis that deformation at MLV is driven by tectonic extension, gravitational loading, plus a component of volume loss at depth, most likely due to cooling and crystallization within the intrusive complex that underlies the edifice. Past InSAR surveys at MLV, and throughout the Cascades, are of variable success due to dense vegetation, snow cover and atmospheric artefacts. In this study, we demonstrate how InSAR may be successfully used in this setting by applying a suite of multitemporal analysis methods that account for atmospheric and orbital noise sources. These methods include: a stacking strategy based upon the noise characteristics of each data set; pixelwise rate-map formation (-RATE) and persistent scatterer InSAR (StaMPS).

Description: Large-scale chemical lateral heterogeneities are inferred in the Earth's lowermost mantle by seismological studies. We explore the model space of thermochemical convection that can maintain reservoirs of dense material for a long period of time, by using similar analysis in 3-D spherical geometry. In this study, we focus on the parameters thought to be important in controlling the stability and structure of primordial dense reservoirs in the lower mantle, including the chemical density contrast between the primordial dense material and the regular mantle material (buoyancy ratio), thermal and chemical viscosity contrasts, volume fraction of primordial dense material and the Clapeyron slope of the phase transition at 660 km depth. We find that most of the findings from the 3-D Cartesian study still apply to 3-D spherical cases after slight modifications. Varying buoyancy ratio leads to different flow patterns, from rapid upwelling to stable layering; and large thermal viscosity contrasts are required to generate long wavelength chemical structures in the lower mantle. Chemical viscosity contrasts in a reasonable range have a second-order role in modifying the stability of the dense anomalies. The volume fraction of the initial primordial dense material does not effect the results with large thermal viscosity contrasts, but has significant effects on calculations with intermediate and small thermal viscosity contrasts. The volume fraction of dense material at which the flow pattern changes from unstable to stable depends on buoyancy ratio and thermal viscosity contrast. An endothermic phase transition at 660 km depth acts as a ‘filter’ allowing cold slabs to penetrate while blocking most of the dense material from penetrating to the upper mantle.

Description: In the literature, the inverted coseismic slip models from seismological and geodetic data for the 2011 Tohoku-Oki earthquake portray significant discrepancies, in particular regarding the intensity and the distribution of the rupture near the trench. For a megathrust earthquake, it is difficult to discern the slip along the shallow part of the fault from the geodetic data, which are often acquired on land. In this paper, we discuss the uncertainties in the slip distribution inversion using the geodetic data for the 2011 Tohoku earthquake and the Fully Bayesian Inversion method. These uncertainties are due to the prior information regarding the boundary conditions at the edges of the fault, the dip subduction angle and the smoothing operator. Using continuous GPS data from the Japan Island, the results for the rigid and free boundary conditions show that they produce remarkably different slip distributions at shallow depths, with the latter producing a large slip exceeding 30 m near the surface. These results indicate that the smoothing operator (gradient or Laplacian schemes) does not severely affect the slip pattern. To better invert the coseismic slip, we then introduce the ocean bottom GPS (OB-GPS) data, which improve the resolution of the shallow part of the fault. We obtain a near-trench slip greater than 40 m that reaches the Earth's surface, regardless of which boundary condition is used. Additionally, we show that using a mean dip angle for the fault as derived from subduction models is adequate if the goal is to invert for the general features of the slip pattern of this megathrust event.

Description: Relative sea level curves contain coupled information about absolute sea level change and vertical lithospheric movement. Such curves may be constructed based on, for example tide gauge data for the most recent times and different types of geological data for ancient times. Correct account for vertical lithospheric movement is essential for estimation of reliable values of absolute sea level change from relative sea level data and vise versa. For modern times, estimates of vertical lithospheric movement may be constrained by data (e.g. GPS-based measurements), which are independent from the relative sea level data. Similar independent data do not exist for ancient times. The purpose of this study is to test two simple inversion approaches for simultaneous estimation of lithospheric uplift rates and absolute sea level change rates for ancient times in areas where a dense coverage of relative sea level data exists and well-constrained average lithospheric movement values are known from, for example glacial isostatic adjustment (GIA) models. The inversion approaches are tested and used for simultaneous estimation of lithospheric uplift rates and absolute sea level change rates in southwest Scandinavia from modern relative sea level data series that cover the period from 1900 to 2000. In both approaches, a priori information is required to solve the inverse problem. A priori information about the average vertical lithospheric movement in the area of interest is critical for the quality of the obtained results. The two tested inversion schemes result in estimated absolute sea level rise of ~1.2/1.3 mm yr –1 and vertical uplift rates ranging from approximately –1.4/–1.2 mm yr –1 (subsidence) to about 5.0/5.2 mm yr –1 if an a priori value of 1 mm yr –1 is used for the vertical lithospheric movement throughout the study area. In case the studied time interval is broken into two time intervals (before and after 1970), absolute sea level rise values of ~0.8/1.2 mm yr –1 (before 1970) and ~2.0 mm yr –1 (after 1970) are found. The uplift patterns resulting from the different inversions suggest that the lithospheric post-GIA response changes near the border between the Danish Basin and the Fennoscandian Shield. The obtained patterns of vertical lithospheric movement rates are comparable to results from other studies based on different and similar data types. Main differences between the inversion results and the results from other studies are caused by factors such as the simplifications included in the inversion approach, such as neglecting local sea level variation caused by the dominant wind patterns, and the a priori values chosen for the vertical uplift rates. The tests of the inversion schemes reveal that realistic values of absolute sea level rise and lithospheric uplift may be simultaneously estimated provided that reliable prior knowledge regarding the overall lithospheric uplift in the study area is available beforehand. In the presented parametrizations, only one absolute sea level change rate value is estimated for each studied time interval while several vertical movement rates are found, and the inverse estimate of absolute sea level change rate is practically insensitive with respect to the choice of a priori value of absolute sea level change, as long as the uncertainty assigned to this a priori value is kept sufficiently high.

Description: In 1356, a magnitude 6–7 earthquake occurred near Basel, in Switzerland. But recent compilations of GPS measurements reveal that measured horizontal deformation rates in northwestern continental Europe are smaller than error bars on the measurements, proving present tectonic activity, if any, is very small in this area. We propose to reconcile these apparently antinomic observations with a mechanical model of the lithosphere that takes into account the geometry of the lithosphere–asthenosphere boundary, assuming that the only loading mechanism is gravity. The lithosphere is considered to be an elastoplastic material satisfying a Von Mises plasticity criterion. The model, which is 400 km long, 360 km wide and 230 km thick, is centred near Belfort in eastern France, with its width oriented parallel to the N145°E direction. It also takes into account the real topography of both the ground surface and that of the Moho discontinuity. Not only does the model reproduce observed principal stress directions orientations, it also identifies a plastic zone that fits roughly the most seismically active domain of the region. Interestingly, a somewhat similar stress map may be produced by considering an elastic lithosphere and an ad-hoc horizontal ‘tectonic’ stress field. However, for the latter model, examination of the plasticity criterion suggests that plastic deformation should have taken place. It is concluded that the present-day stress field in this region is likely controlled by gravity and rheology, rather than by active Alpine tectonics.

Description: We have investigated variations in transition zone thickness under the Borborema Province of NE Brazil by migrating and stacking teleseismic P -wave receiver functions at 32 seismic stations in the region. The Borborema Province represents the western portion of a larger Neoproterozoic mobile belt that occupied much of northern Gondwana, where extensional processes in the Mesozoic lead to the formation of a number of intracontinental basins and ultimately continental breakup. Episodes of intraplate volcanism and uplift marked the evolution of the Province during the Cenozoic, but it is unclear whether those episodes originated from shallow or deep-seated magmatic sources. On one hand, the elliptical shape of the uplifted area, the stress pattern of the Cenozoic deformation and the time overlap between uplift and volcanism suggest doming from thermal activation due to a deep-seated mantle plume. On the other hand, geochronological dates of volcanic bodies in the Province are better understood if resulting from lithospheric erosion by a shallow, small-scale convection cell. Large temperature anomalies are expected to be associated with mantle upwellings, and constraints on the depth extent of the upwellings can be obtained from transition zone thickness. Thinning of the transition zone with respect to its nominal 250 km value is considered diagnostic for positive temperature anomalies, while thickening is considered diagnostic for negative anomalies. Our results show that transition zone thickness is normal, around 250 km, throughout the Province and suggest that thermal perturbations—if present—are confined to the upper mantle. We argue that our results are consistent with a local, shallow magmatic source for the Cenozoic intraplate volcanism of the Borborema Province, although other proposed scenarios—such as channeling of upwelling plume material along lithospheric thin spots—cannot be ruled out with our analysis.

Description: Mammalian synthetic biology may provide novel therapeutic strategies, help decipher new paths for drug discovery and facilitate synthesis of valuable molecules. Yet, our capacity to genetically program cells is currently hampered by the lack of efficient approaches to streamline the design, construction and screening of synthetic gene networks. To address this problem, here we present a framework for modular and combinatorial assembly of functional (multi)gene expression vectors and their efficient and specific targeted integration into a well-defined chromosomal context in mammalian cells. We demonstrate the potential of this framework by assembling and integrating different functional mammalian regulatory networks including the largest gene circuit built and chromosomally integrated to date (6 transcription units, 27kb) encoding an inducible memory device. Using a library of 18 different circuits as a proof of concept, we also demonstrate that our method enables one-pot/single-flask chromosomal integration and screening of circuit libraries. This rapid and powerful prototyping platform is well suited for comparative studies of genetic regulatory elements, genes and multi-gene circuits as well as facile development of libraries of isogenic engineered cell lines.

Description: Standard techniques for computed tomography imaging are not directly applicable to a carbonate rock because of the geometric complexity of its pore space. In this study, we first characterized the pore structure in Majella limestone with 30 per cent porosity. Microtomography data acquired on this rock was partitioned into three distinct domains: macropores, solid grains, and an intermediate domain made up of voxels of solid embedded with micropores below the resolution. A morphological analysis of the microtomography images shows that in Majella limestone both the solid and intermediate domains are interconnected in a manner similar to that reported previously in a less porous limestone. We however show that the macroporosity in Majella limestone is fundamentally different, in that it has a percolative backbone which may contribute significantly to its permeability. We then applied for the first time 3-D-volumetric digital image correlation (DIC) to characterize the mode of mechanical failure in this limestone. Samples were triaxially deformed over a wide range of confining pressures. Tomography imaging was performed on these samples before and after deformation. Inelastic compaction was observed at all tested pressures associated with both brittle and ductile behaviors. Our DIC analysis reveals the structure of compacting shear bands in Majella limestone deformed in the transitional regime. It also indicates an increase of geometric complexity with increasing confinement—from a planar shear band, to a curvilinear band, and ultimately to a diffuse multiplicity of bands, before shear localization is inhibited as the failure mode completes the transition to delocalized cataclastic flow.

Description: The Pyrenean mountain range is a slowly deforming belt with continuous and moderate seismic activity. To quantify its deformation field, we present the velocity field estimated from a GPS survey of the Pyrenees spanning 18 yr. The PotSis and ResPyr networks, including a total of 85 GPS sites, were installed and first measured in 1992 and 1995–1997, respectively, and remeasured in 2008 and 2010. We obtain a deformation field with velocities less than 1 mm yr –1 across the range. The estimated velocities for individual stations do not differ significantly from zero with 95 per cent confidence. Even so, we estimate a maximum extensional horizontal strain rate of 2.0 ± 1.7 nanostrain per year in a N–S direction in the western part of the range. We do not interpret the vertical displacements due to their large uncertainties. In order to compare the horizontal strain rates with the seismic activity, we analyse a set of 194 focal mechanisms using three methods: (i) the ‘r’ factor relating their P and T axes, (ii) the stress tensors obtained by fault slip inversion and (iii) the strain-rate tensors. Stress and strain-rate tensors are estimated for: (i) the whole data set, (ii) the eastern and western parts of the range separately, and (iii) eight zones, which are defined based on the seismicity and the tectonic patterns of the Pyrenees. Each of these analyses reveals a lateral variation of the deformation style from compression and extension in the east to extension and strike-slip in the west of the range. Although the horizontal components of the strain-rate tensors estimated from the seismic data are slightly smaller in magnitude than those computed from the GPS velocity field, they are consistent within the 2 uncertainties. Furthermore, the orientations of their principal axes agree with the mapped active faults.

Description: In general, observations are normally considered to refer to an epoch in time, however, observations take time. During this time span temporal variations of the observable alias the measurement. Similar phenomenon can be defined in the space domain as well: data treated to refer to a geographical location often contains integrated information of the surroundings. In each case the appropriate signal content can partially be recovered by desmoothing the averaged data. The present study delivers the theoretical foundation of a desmoothing method, and suggests its use on different applications in geodesy. The theoretical formulation of the desmoothing has been derived for 1-D and 2-D signals, the latter is interpreted on a plain and also on a sphere. The presented case studies are less elaborated, but intended to demonstrate the need and usefulness of the desmoothing tool.

Description: The resistivity structure of the lithospheric mantle beneath the Proterozoic Grenville Province in southern Ontario, Canada is investigated using 84 magnetotelluric (MT) sites divided into four profiles. Depth-based regional geoelectric dimensionality analyses of the MT responses indicate that the mantle lithosphere north of Lake Ontario can be subdivided into upper (45–150 km) and deeper (〉200 km) lithospheric mantle layers with regional strike azimuths of N85°E (±5°) and N65°E (±5°), respectively. MT responses from the Grenville Front and the northwest part of the Central Gneiss Belt are compatible with the presence of 2-D resistivity structures but farther to the southeast, in the southeast part of the Central Gneiss Belt and Central Metasedimentary Belt, they suggest the presence of localized 3-D structures. 2-D inversion of distortion-free MT responses images a large scale very resistive (〉20 000  m) region that extends 300 km southeast of the Grenville Front and for at least 800 km along-strike in the lithospheric mantle beneath the Grenville Province. This feature is interpreted to be Superior Province lithosphere and the corresponding N85°E geoelectric strike to be associated with the fabric of the Superior Province. The base of the resistor reaches depths of 280 km on two of the three MT profiles north of Lake Ontario and this depth is interpreted to be the base of the lithosphere. A large region of enhanced conductivity in the lower lithosphere, spatially correlated with decreased seismic velocity, is bounded to the northwest by a subvertical resistivity anomaly located near the Kirkland Lake and Cobalt kimberlite fields. The enhanced conductivity in the lower lithosphere is attributed to refertilization by fluids associated with Cretaceous kimberlite magmatism and can be explained by water content in olivine of 50 wt ppm in background areas with higher values in a localized anomaly beneath the kimberlite fields. Farther to the southeast the resistivity models include a lithospheric conductor between 100 and 150 km depth beneath the Central Metasedimentary Belt. The enhanced conductivity is attributed to grain boundary graphite films, associated with the Cretaceous kimberlitic magmatic process, or to water and carbon, introduced into the mantle during the pre-Grenvillian tectonism.

Description: Displacements and stress-field changes associated with earthquakes, volcanoes, landslides and human activity are often simulated using numerical models in an attempt to understand the underlying processes and their governing physics. The application of elastic dislocation theory to these problems, however, may be biased because of numerical instabilities in the calculations. Here, we present a new method that is free of artefact singularities and numerical instabilities in analytical solutions for triangular dislocations (TDs) in both full-space and half-space. We apply the method to both the displacement and the stress fields. The entire 3-D Euclidean space $\mathbb {R}^{3}$ is divided into two complementary subspaces, in the sense that in each one, a particular analytical formulation fulfils the requirements for the ideal, artefact-free solution for a TD. The primary advantage of the presented method is that the development of our solutions involves neither numerical approximations nor series expansion methods. As a result, the final outputs are independent of the scale of the input parameters, including the size and position of the dislocation as well as its corresponding slip vector components. Our solutions are therefore well suited for application at various scales in geoscience, physics and engineering. We validate the solutions through comparison to other well-known analytical methods and provide the MATLAB codes.

Description: Models of the glacial isostatic adjustment process, which is dominated by the influence of the Late Pleistocene cycle of glaciation and deglaciation, depend on two fundamental inputs: a history of ice-sheet loading and a model of the radial variation of mantle viscosity. These models may be tested and refined by comparing their local predictions of relative sea level history to geological inferences based upon appropriate sea level indicators. The U.S. Atlantic coast is a region of particular interest in this regard, due to the fact that data from the length of this coast provides a transect of the forebulge associated with the former Laurentide ice sheet. High-quality relative sea level histories from this region are employed herein to explore the ability of current models of mantle viscosity to explain the inferred evolution of relative sea level that have accompanied forebulge collapse following deglaciation. Existing misfits are characterized, and alternatives are explored for their reconciliation. It is demonstrated that a new model of mantle viscosity, referred to herein as VM6, when coupled with the latest model of deglaciation history ICE-6G_C, is able to eliminate the majority of these misfits, while continuing to reconcile a wide range of other important geophysical observables, as well as additional relative sea level data from the North American. West coast which also record the collapse of the forebulge but which have not been employed in tuning the viscosity profile to enable ICE-6G_C (VM6) to fit the East coast data set.

Description: The origin of shear wave splitting (SWS) in the mantle beneath Iceland is examined using numerical models that simulate 3-D mantle flow and the development of seismic anisotropy due to lattice-preferred orientation (LPO). Using the simulated anisotropy structure, we compute synthetic SKS waveforms, invert them for fast polarization directions and split times, and then compare the predictions with the results from three observational studies of Iceland. Models that simulate a mantle plume interacting with the Mid-Atlantic Ridge in which the shallow-most mantle has a high viscosity due to the extraction of water with partial melting, or in which C-type olivine LPO fabric is present due to high water content in the plume, produce the largest chi-squared misfits to the SWS observations and are thus rejected. Models of a low-viscosity mantle plume with A-type olivine fabric everywhere, or with the added effects of E-type fabric in the plume below the solidus produce lower misfits. The lowest misfits are produced by models that include a rapid (~50 km Myr –1 ) northward regional flow (NRF) in the mid-upper mantle, either with or without a plume. NRF was previously indicated by a receiver function study and a regional tomography study, and is shown here to be a major cause of the azimuthal anisotropy beneath Iceland. The smallest misfits for the models with both a plume and NRF are produced when LPO forms above depths of 300–400 km, which, by implication, also mark the depths above which dislocation creep dominates over diffusion creep. This depth of transition between dislocation and diffusion creep is greater than expected beneath normal oceanic seafloor, and is attributed to the unusually rapid strain rates associated with an Iceland plume and the NRF.

Description: The resistivity structure of the lithospheric mantle beneath the Proterozoic Grenville Province in southern Ontario, Canada is investigated using 84 magnetotelluric (MT) sites divided into four profiles. Depth-based regional geoelectric dimensionality analyses of the MT responses indicate that the mantle lithosphere north of Lake Ontario can be subdivided into upper (45–150 km) and deeper (〉200 km) lithospheric mantle layers with regional strike azimuths of N85°E (±5°) and N65°E (±5°), respectively. MT responses from the Grenville Front and the northwest part of the Central Gneiss Belt are compatible with the presence of 2-D resistivity structures but farther to the southeast, in the southeast part of the Central Gneiss Belt and Central Metasedimentary Belt, they suggest the presence of localized 3-D structures. 2-D inversion of distortion-free MT responses images a large scale very resistive (〉20 000  m) region that extends 300 km southeast of the Grenville Front and for at least 800 km along-strike in the lithospheric mantle beneath the Grenville Province. This feature is interpreted to be Superior Province lithosphere and the corresponding N85°E geoelectric strike to be associated with the fabric of the Superior Province. The base of the resistor reaches depths of 280 km on two of the three MT profiles north of Lake Ontario and this depth is interpreted to be the base of the lithosphere. A large region of enhanced conductivity in the lower lithosphere, spatially correlated with decreased seismic velocity, is bounded to the northwest by a subvertical resistivity anomaly located near the Kirkland Lake and Cobalt kimberlite fields. The enhanced conductivity in the lower lithosphere is attributed to refertilization by fluids associated with Cretaceous kimberlite magmatism and can be explained by water content in olivine of 50 wt ppm in background areas with higher values in a localized anomaly beneath the kimberlite fields. Farther to the southeast the resistivity models include a lithospheric conductor between 100 and 150 km depth beneath the Central Metasedimentary Belt. The enhanced conductivity is attributed to grain boundary graphite films, associated with the Cretaceous kimberlitic magmatic process, or to water and carbon, introduced into the mantle during the pre-Grenvillian tectonism.

Description: Using dynamic rupture models of a right-lateral fault embedded in an elastic or elastoplastic 3-D medium, we investigate elastic and inelastic responses of compliant fault zones to nearby earthquake ruptures. We particularly examine effects of fault zone depth, width, shape and rigidity reduction on the surface displacement field. Our results from elastic models show that deeper and wider fault zones generally result in larger residual displacements. However, for shallow fault zones, the vertical residual displacement is insensitive to or even decreases with fault zone width. The width of horizontal displacement anomalies across a fault zone is only indicative of the fault zone width near the Earth's surface. There are trade-off effects among fault zone depth, width, shape and rigidity reduction on the amplitude of surface residual displacements. Our elastoplastic models show that plastic strain can occur along the entire fault zone near the Earth's surface and in the extensional quadrant at depth, if fault zone rocks are close to failure before a nearby earthquake happens. Compared with results from elastic models, plastic strain near the Earth's surface generally enhances surface displacements of a fault zone and does not change the trend of effects of fault zone depth and width, while plastic strain at depth can result in reduced retrograde motion or sympathetic motion across the fault zone, and introduce complexities in effects of fault zone depth and width. Sympathetic horizontal motion more likely occurs across a narrow fault zone with inelastic response at depth. Vertical motion in the extensional quadrant may actually decrease with fault zone width in elastoplastic models. Sympathetic horizontal motion, or small retrograde horizontal motion in conjunction with large vertical motion above a fault zone is indicative of inelastic response of a fault zone at depth.

Description: The Algerian margin formed through back-arc opening of the Algerian basin (Mediterranean Sea) resulting from the roll-back of the Tethyan slab. Recent geophysical data acquired along the Algerian margin showed evidence of active or recent compressive deformation in the basin due to the ongoing Africa–Eurasia convergence. Published data from four wide-angle seismic profiles have allowed imaging the deep structure of the Algerian margin and its adjacent basins. In this study, we converted these velocity models into density models, then into isostatic anomalies. This allowed us to image an isostatic disequilibrium (relative to a local isostasy model) reaching a maximum amplitude at the margin toe. Converting isostatic anomalies into Moho depth variations shows that the Moho extracted from wide-angle seismic data is deeper than the one predicted by a local isostasy model in the oceanic domain, and shallower than it in the continental domain. These anomalies can be interpreted by opposite flexures of two plates separated by a plate boundary located close to the margin toe. We use a finite element model to simulate the lithospheric flexure. The amplitude of the equivalent vertical Moho deflection is larger in the central part of the study area (6–7 km) than on the easternmost and westernmost profiles (3 km). The effective elastic thickness used to best match the computed deflection is always extremely low (always less than 10 km) and probably reflects the relatively low strength of the lithosphere close to the plate boundary. Comparison with other wide-angle seismic profiles across an active and a passive margin show that the North Algerian margin displays isostatic anomalies close to that of an active margin. Finally, plate flexure is highest at the southern tip of the ocean-continent transition, possibly indicating that a former passive margin detachment is reactivated as a crustal scale reverse fault pre-dating a future subduction.

Description: Wide-angle reflection/refraction seismic profiles were recorded across the Cyprus Arc, the plate boundary between the African Plate and the Aegean–Anatolian microplate, from the Eratosthenes Seamount to the Hecataeus Rise immediately south of Cyprus. The resultant models were able to resolve detail of significant lateral velocity variations, though the deepest crust and Moho are not well resolved from the seismic data alone. Conclusions from the modelling suggest that (i) Eratosthenes Seamount consists of continental crust but exhibits a laterally variable velocity structure with a thicker middle crust and thinner lower crust to the northeast; (ii) the Hecataeus Rise has a thick sedimentary rock cover on an indeterminate crust (likely continental) and the crust is significantly thinner than Eratosthenes Seamount based on gravity modelling; (iii) high velocity basement blocks, coincident with highs in the magnetic field, occur in the deep water between Eratosthenes and Hecataeus, and are separated and bounded by deep low-velocity troughs and (iv) one of the high velocity blocks runs parallel to the Cyprus Arc, while the other two appear linked based on the magnetic data and run NW–SE, parallel to the margin of the Hecataeus Rise. The high velocity block beneath the edge of Eratosthenes Seamount is interpreted as an older magmatic intrusion while the linked high velocity blocks along Hecataeus Rise are interpreted as deformed remnant Tethyan oceanic crust or mafic intrusives from the NNW–SSE oriented transform margin marking the northern boundary of Eratosthenes Seamount. Eratosthenes Seamount, the northwestern limit of rifted continental crust from the Levant Margin, is part of a jagged rifted margin transected by transform faults on the northern edge of the lower African Plate that is being obliquely subducted under the Aegean–Anatolian upper plate. The thicker crust of Eratosthenes Seamount may be acting as an asperity on the subducting slab, locally locking up subduction of the Cyprus Arc on its northern margin, while deformed Tethyan oceanic crust remains trapped between its northeastern margin and the Hecataeus Rise.

Description: We present an up-to-date high resolution picture of the ongoing crustal deformation field of Italy, based on an extensive combination of permanent and non-permanent GPS observations carried out since 1994. In addition, we present an updated map of contemporary S Hmax orientations computed by a multidisciplinary data set of well-constrained stress indicators, including both published results and novel analyses. The comparison of stress and geodetic strain-rates directions reveals that both patterns are near-parallel over a large part of the investigated area, highlighting that crustal stress and surface deformation are driven by the same mechanism. The comparison of the azimuthal patterns of surface strain and mantle deformation shows a modest correlation on the Alps and a low correlation along the Apennines chain and the Calabro-Peloritan Arc. Along the Apennines chain, this feature suggests the occurrence of significant strain partitioning and crust–mantle mechanical decoupling. Along the Calabro-Peloritan Arc, the apparent low correlation reflects a different mantle–crust mechanism of deformation to the ongoing subduction and rollback of the Ionian slab. In addition, the superposition of regional/local effects related to second-order sources (crustal lateral density changes, strength contrasts), which at regional/local scale modulate the crustal stress/strain-rate pattern, cannot be ruled out.

Description: A series of linear analysis was performed on the onset of thermal convection of highly compressible fluids, in order to deepen the fundamental insights into the mantle convection of massive super-Earths in the presence of strong adiabatic compression. We consider the temporal evolution (growth or decay) of an infinitesimal perturbation superimposed to a highly compressible fluid which is in a hydrostatic (motionless) and conductive state in a basally heated horizontal layer. As a model of pressure-dependence in material properties, we employed an exponential decrease in thermal expansivity α and exponential increase in (reference) density with depth. The linearized equations for conservation of mass, momentum and internal (thermal) energy are numerically solved for the critical Rayleigh number as well as the vertical profiles of eigenfunctions for infinitesimal perturbations. The above calculations are repeatedly carried out by systematically varying (i) the dissipation number (Di), (ii) the temperature at the top surface and (iii) the magnitude of pressure-dependence in α and . Our analysis demonstrated that the onset of thermal convection is strongly affected by the adiabatic compression, in response to the changes in the static stability of thermal stratification in the fluid layer. For sufficiently large Di where a thick sublayer of stable stratification develops in the layer, for example, the critical Rayleigh number explosively increases with Di, together with drastic decreases in the length scales of perturbations both in vertical and horizontal directions. In particular, for very large Di, a thick ‘stratosphere’ occurs in the fluid layer where the vertical motion is significantly suppressed, resulting in a shrink of the incipient convection in a thin sublayer of unstable thermal stratification. In addition, when Di exceeds a threshold value above which a thermal stratification becomes stable in the entire layer, no perturbation is allowed to grow with time regardless of the Rayleigh number and/or the horizontal wavelength. We also found that the effect of adiabatic compression becomes prominent for higher temperature at the top surface of the fluid layer. These findings may imply the crucial importance of adiabatic compression in understanding the dynamics and evolution of the mantles of massive super-Earths, particularly for those orbiting their parent stars very closely.

Description: Between 2011 and 2013, two dense transects were deployed across the central and western Pyrenees to get better constraints on the deep lithospheric architecture and discriminate the competing models of the structure and formation of the Pyrenees. Each transect recorded the regional and global seismicity during a period of approximately 1 yr. Here, we exploit the records of teleseismic compressional waves and of their conversions to shear waves on internal discontinuities in order to map lithospheric interfaces beneath the two transects. The migrated sections, obtained by performing common conversion point stacks, are in remarkable agreement with the results of the ECORS-Pyrenees and ECORS-Arzacq deep seismic surveys. However, the migrations of converted waves reveal new details of the deep lithospheric architecture that could not be seen with the active source experiments. The new images provide clear and definite evidence for the subduction of a thinned Iberian crust down to at least ~70 km depth, a result that has important implications for the formation of the Pyrenees. The subduction of the Iberian lithosphere leads to reconsider the amount of convergence between Iberia and Europe during the Cenozoic. A recent regional P -wave tomography, relying on the data of the PYROPE and IBERARRAY temporary experiments, revealed the segmentation of lithospheric structures by inherited Hercynian NE–SW transfer faults that were reactivated during the Albian rifting. Our migration images are consistent with this model, and give further support to the idea that the Pyrenees were produced by the tectonic inversion of a segmented hyperextended rift that was buried by subduction beneath the European Plate.

Description: Extracting geophysical signals from Global Positioning System (GPS) coordinate time-series is a well-established practice that has led to great insights into how the Earth deforms. Often small discontinuities are found in such time-series and are traceable to either broad-scale deformation (i.e. earthquakes) or discontinuities due to equipment changes and/or failures. Estimating these offsets accurately enables the identification of coseismic deformation estimates in the former case, and the removal of unwanted signals in the latter case which then allows tectonic rates to be estimated more accurately. We develop a method to estimate accurately discontinuities in time series of GPS positions at specified epochs, based on a so-called ‘offset series’. The offset series are obtained by varying the amount of GPS data before and after an event while estimating the offset. Two methods, a mean and a weighted mean method, are then investigated to produce the estimated discontinuity from the offset series. The mean method estimates coseismic offsets without making assumptions about geophysical processes that may be present in the data (i.e. tectonic rate, seasonal variations), whereas the weighted mean method includes estimating coseismic offsets with a model of these processes. We investigate which approach is the most appropriate given certain lengths of available data and noise within the time-series themselves. For the Sumatra–Andaman event, with 4.5 yr of pre-event data, we show that between 2 and 3 yr of post-event data are required to produce accurate offset estimates with the weighted mean method. With less data, the mean method should be used, but the uncertainties of the estimated discontinuity are larger.

Description: Complications arise in the interpretation of gravity fields because of interference from systematic degradations, such as boundary blurring and distortion. The major sources of these degradations are the various systematic errors that inevitably occur during gravity field data acquisition, discretization and geophysical forward modelling. To address this problem, we evaluate deconvolution method that aim to detect the clear horizontal boundaries of anomalous sources by the suppression of systematic errors. A convolution-based multilayer projection model, based on the classical 3-D gravity field forward model, is innovatively derived to model the systematic error degradation. Our deconvolution algorithm is specifically designed based on this multilayer projection model, in which three types of systematic error are defined. The degradations of the different systematic errors are considered in the deconvolution algorithm. As the primary source of degradation, the convolution-based systematic error is the main object of the multilayer projection model. Both the random systematic error and the projection systematic error are shown to form an integral part of the multilayer projection model, and the mixed norm regularization method and the primal-dual optimization method are therefore employed to control these errors and stabilize the deconvolution solution. We herein analyse the parameter identification and convergence of the proposed algorithms, and synthetic and field data sets are both used to illustrate their effectiveness. Additional synthetic examples are specifically designed to analyse the effects of the projection systematic error, which is caused by the uncertainty associated with the estimation of the impulse response function.

Description: In this paper, we propose an approach to compute the coseismic Earth's volume change based on a spherical-Earth elastic dislocation theory. We present a general expression of the Earth's volume change for three typical dislocations: the shear, tensile and explosion sources. We conduct a case study for the 2004 Sumatra earthquake ( M w 9.3), the 2010 Chile earthquake ( M w 8.8), the 2011 Tohoku-Oki earthquake ( M w 9.0) and the 2013 Okhotsk Sea earthquake ( M w 8.3). The results show that mega-thrust earthquakes make the Earth expand and earthquakes along a normal fault make the Earth contract. We compare the volume changes computed for finite fault models and a point source of the 2011 Tohoku-Oki earthquake ( M w 9.0). The big difference of the results indicates that the coseismic changes in the Earth's volume (or the mean radius) are strongly dependent on the earthquakes’ focal mechanism, especially the depth and the dip angle. Then we estimate the cumulative volume changes by historical earthquakes ( M w ≥ 7.0) since 1960, and obtain an Earth mean radius expanding rate about 0.011 mm yr –1 .

Description: A major challenge in metabolic engineering and synthetic biology is to balance the flux of an engineered heterologous metabolic pathway to achieve high yield and productivity in a target organism. Here, we report a simple, efficient and programmable approach named ‘customized optimization of metabolic pathways by combinatorial transcriptional engineering (COMPACTER)’ for rapid tuning of gene expression in a heterologous pathway under distinct metabolic backgrounds. Specifically, a library of mutant pathways is created by de novo assembly of promoter mutants of varying strengths for each pathway gene in a target organism followed by high-throughput screening/selection. To demonstrate this approach, a single round of COMPACTER was used to generate both a xylose utilizing pathway with near-highest efficiency and a cellobiose utilizing pathway with highest efficiency that were ever reported in literature for both laboratory and industrial yeast strains. Interestingly, these engineered xylose and cellobiose utilizing pathways were all host-specific. Therefore, COMPACTER represents a powerful approach to tailor-make metabolic pathways for different strain backgrounds, which is difficult if not impossible to achieve by existing pathway engineering methods.

Description: We developed a highly scalable ‘shotgun’ DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster.

Description: We present the crustal resistivity structure of the Pamir and Southern Tian Shan orogenic belts at the northwestern promontory of the India–Asia collision zone. The magnetotelluric (MT) data were recorded along a roughly north–south trending, 350 km long corridor from the Pamir Plateau in southern Tajikistan across the Pamir frontal ranges, the Alai Valley and the southwestern Tian Shan to Osh in the Kyrgyz part of the Fergana Basin. In total, we measured at 178 sites, whereof 26 combine broad band and long period recordings. One of the most intriguing features of the 2-D and 3-D inversion results is a laterally extended zone of high electrical conductivity below the Pamir Plateau, with resistivities below 1 m, starting at a depth of ~10–15 km. The high conductivity can be explained with the presence of partially molten rocks at middle to lower crustal levels, possibly related to ongoing migmatization and/or middle/lower crustal flow underneath the Southern Pamir. This interpretation is consistent with a low velocity zone found from local earthquake tomography, relatively high v p / v s ratios, elevated surface heat flow, and thermomechanical modelling suggesting that melting temperatures are reached in the felsic middle crust. In the upper crust of the Pamir and Tian Shan, the Palaeozoic–Mesozoic suture zones appear as electrically conductive, whereas the compact metamorphic rocks of the Muskol-Shatput Dome of the Central Pamir are highly resistive. The intra-montane basin of the Alai Valley—sandwiched between the Pamir and Tian Shan—exhibits a generally conductive upper crust that bifurcates into two conductors at depth. One of them connects to the active Main Pamir Thrust, which is absorbing most of today's convergence between the Pamir and the Tian Shan. Several deeper zones of high conductivity in the middle and lower crust of Central and Northern Pamir likely record fluid release due to metamorphism associated with active continental subduction/delamination.

Description: A new model of the deglaciation history of Antarctica over the past 25 kyr has been developed, which we refer to herein as ICE-6G_C (VM5a). This revision of its predecessor ICE-5G (VM2) has been constrained to fit all available geological and geodetic observations, consisting of: (1) the present day uplift rates at 42 sites estimated from GPS measurements, (2) ice thickness change at 62 locations estimated from exposure-age dating, (3) Holocene relative sea level histories from 12 locations estimated on the basis of radiocarbon dating and (4) age of the onset of marine sedimentation at nine locations along the Antarctic shelf also estimated on the basis of 14 C dating. Our new model fits the totality of these data well. An additional nine GPS-determined site velocities are also estimated for locations known to be influenced by modern ice loss from the Pine Island Bay and Northern Antarctic Peninsula regions. At the 42 locations not influenced by modern ice loss, the quality of the fit of postglacial rebound model ICE-6G_C (VM5A) is characterized by a weighted root mean square residual of 0.9 mm yr –1 . The Southern Antarctic Peninsula is inferred to be rising at 2 mm yr –1 , requiring there to be less Holocene ice loss there than in the prior model ICE-5G (VM2). The East Antarctica coast is rising at approximately 1 mm yr –1 , requiring ice loss from this region to have been small since Last Glacial Maximum. The Ellsworth Mountains, at the base of the Antarctic Peninsula, are inferred to be rising at 5–8 mm yr –1 , indicating large ice loss from this area during deglaciation that is poorly sampled by geological data. Horizontal deformation of the Antarctic Plate is minor with two exceptions. First, O'Higgins, at the tip of the Antarctic Peninsula, is moving southeast at a significant 2 mm yr –1 relative to the Antarctic Plate. Secondly, the margins of the Ronne and Ross Ice Shelves are moving horizontally away from the shelf centres at an approximate rate of 0.8 mm yr –1 , in viscous response to the early Holocene unloading of ice from the current locations of the ice shelf centers. ICE-6G_C (VM5A) fits the horizontal observations well (wrms residual speed of 0.7 mm yr –1 ), there being no need to invoke any influence of lateral variation in mantle viscosity. ICE-6G_C (VM5A) differs in several respects from the recently published W12A model of Whitehouse et al. First, the upper-mantle viscosity in VM5a is 5 10 20 Pa s, half that in W12A. The VM5a profile, which is identical to that inferred on the basis of the Fennoscandian relaxation spectrum, North American relative sea level histories and Earth rotation constraints, when coupled with the revised ICE-6G_C deglaciation history, fits all of the available constraints. Secondly, the net contribution of Antarctica ice loss to global sea level rise is 13.6 m, 2/3 greater than the 8 m in W12A. Thirdly, ice loss occurs quickly from 12 to 5 ka, and the contribution to global sea level rise during Meltwater Pulse 1B (11.5 ka) is large (5 m), consistent with sedimentation constraints from cores from the Antarctica ice shelf. Fourthly, in ICE-6G_C there is no ice gain in the East Antarctica interior, as there is in W12A. Finally, the new model of Antarctic deglaciation reconciles the global constraint upon the global mass loss during deglaciation provided by the Barbados record of relative sea level history when coupled with the Northern Hemisphere counterpart of this new model.

Description: We propose to test if gravimetry can prove useful in discriminating different models of long-term deep crustal processes in the case of the Taiwan mountain belt. We discuss two existing tectonic models that differ in the deep processes proposed to sustain the long-term growth of the orogen. One model assumes underplating of the uppermost Eurasian crust with subduction of the deeper part of the crust into the mantle. The other one suggests the accretion of the whole Eurasian crust above crustal-scale ramps, the lower crust being accreted into the collisional orogen. We compute the temporal gravity changes caused only by long-term rock mass transfers at depth for each of them. We show that the underplating model implies a rate of gravity change of –6 x 10 –2 μGal yr –1 , a value that increases to 2 x 10 –2 μGal yr –1 if crustal subduction is neglected. If the accretion of the whole Eurasian crust occurs, a rate of 7 x 10 –2 μGal yr –1 is obtained. The two models tested differ both in signal amplitude and spatial distribution. The yearly gravity changes expected by long-term deep crustal mass processes in Taiwan are two orders of magnitude below the present-day uncertainty of land-based gravity measurements. Assuming that these annually averaged long-term gravity changes will linearly accumulate with ongoing mountain building, multidecadal time-series are needed to identify comparable rates of gravity change. However, as gravity is sensitive to any mass redistribution, effects of short-term processes such as seismicity and surface mass transfers (erosion, sedimentation, ground-water) may prevent from detecting any long-term deep signal. This study indicates that temporal gravity is not appropriate for deciphering the long-term deep crustal processes involved in the Taiwan mountain belt.

Description: The Himalaya is the result of the on-going convergence and collision of India and Asia. The internal configuration and processes that govern the rise of the Himalayan Mountains and Tibetan Plateau are crucial to understand continental collision zones. However, knowledge of the prior configuration of the colliding plates is equally important, since inherited (pre-orogenic/basement) structures can undeniably influence the development of the orogenic architecture throughout the orogen's cycle of collision and eventual collapse. Three northeast-trending palaeotopographic ridges of faulted Precambrian Indian basement underlie the Ganga basin south of the Himalaya. Our paper illustrates a crustal-scale fault origin for these ridges and succeeds in determining how far north beneath the Himalayan system they extend and how they ultimately govern the location of upper crustal faults in southern Tibet. Spectrally filtered EGM2008 Bouguer gravity data and edges in its horizontal gradient at different source depths (‘gravity worms’) over northern Peninsular India, the Himalaya and southern Tibet reveal several continuous Himalayan cross-strike discontinuities interpreted to represent crustal faults. Gravity lineaments in Peninsular India coincide with edges of the Precambrian basement ridges and megakinks up to 100 km wide develop in foreland cover sequences between the interpreted basement faults. The interpreted basement faults project northward beneath the Himalayan system and southern Tibet. Our results suggest that several active Himalayan cross-strike faults, such as the ones related to many graben in southern Tibet, are rooted in the underplated Indian lower crust or step en échelon along interpreted basement faults. Our interpretation thus suggests that south Tibet graben are spatially related to deep-seated crustal-scale faults rooted in the underplated Indian crust. These major discontinuities partition the Himalayan range into distinct zones, and could ultimately contribute to lateral variability in tectonic evolution along the orogen's strike.

Description: We explore the impact of deep ductile shear zones on post-seismic deformation following a finite length strike-slip earthquake. We show that the pattern of post-seismic vertical surface deformation surrounding the fault is a discriminant for the existence of high viscosities immediately below the seismogenic layer, regardless of whether the model contains purely distributed creep or also includes a component of localized creep at subseismogenic depths. Post-seismic deformation characterized by initially fast relaxation followed by a slower relaxation is predicted by models that include both localized creep in a subseismogenic shear zone and distributed creep in the surrounding region, even if they only contain steady Maxwell viscoelasticity. This post-seismic deformation is similar to that in models that approximate the ductile lithosphere and/or asthenosphere with Burgers viscoelasticity. We find that the post-seismic deformation following the 1997 M w 7.6 Manyi, China, earthquake, is consistent with a post-seismic model composed of a lower Maxwell viscoelastic region with viscosity 10 19 Pa s and a 5 km wide, Maxwell viscoelastic shear zone with viscosity 10 18 Pa s beneath the fault.