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  • Organic Chemistry  (68,935)
  • Life and Medical Sciences  (30,791)
  • Genetics  (2,233)
  • Wiley-Blackwell  (99,730)
  • FISON  (5)
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  • 1
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    FISON | Lagos, Nigeria
    In:  http://aquaticcommons.org/id/eprint/23241 | 19325 | 2018-03-18 15:26:11 | 23241 | Fisheries Society of Nigeria
    Publication Date: 2021-07-13
    Description: Cephalic abnormality in nine mating groups involving Clarias gariepinus with cephalic abnormality, non Cephalic and Maiduguri origin were investigated to determine the level and the genetic basis of the occurrences and its aquaculture implication. The highest mean percentage survival in a Cephalic crossed with non cephalic group was 68.6% whereas the last mean percentage survival was 25.7% in the group of Maiduguri cross with Maiduguri parent. Cephalic abnormality was observed in the crosses with both female and male cephalic with a total frequency ranging from 0 % to 70.8. %. The least number of cephalic abnormality was 18.5% which involved a cross of non cephalic with cephalic, and the highest was 70.8%. This level of cephalic abnormality shows that the defect was hereditary. This result implies that, the genetic factor is a major contributor in the feature of cephalic abnormality in Clarias gariepinus.
    Description: Includes:-1 table.;3 appendixes.;11 refs.
    Keywords: Aquaculture ; Fisheries ; Clarias gariepinus ; Nigeria ; freshwater environment ; Diseases ; Deformation ; Fish culture ; Genetics ; Abnormalities ; Genetic abnormalities
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 199 - 208
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  • 2
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    FISON | Akure (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/23373 | 19325 | 2018-04-10 16:09:38 | 23373 | Fisheries Society of Nigeria
    Publication Date: 2021-07-14
    Description: The contribution of tilapia aquaculture in Nigeria to world output is negligible due to stunting, poor market value among others. This paper evaluates the aquacultural credentials of tilapia, some genetic improvement technology in cultured tilapia, namely, ploidy, hormonal sex reversal. transgenic, hybridization, and the necessity of Genetic Improvement in accelerating tilapia production in Nigeria. Investigation reveals the presence of O. niloticus with the highest growth perfermancc index (~h1=3.11) for Lake Kanji which indicates high growth potential in suitable culture environment and could serve as a good starting point for genetic development. The presence of ”wesafu”, an ecotype cichlid, endemic to Epe lagoon, Lagos, which grows to 1500g in the wild, appears to be an excellent candidate for genetic improvement of a commercial strain for the growing aquaculture industry. Tilapia Genetic improvement in Nigeria is faced with a number of setbacks. This includes short- term, scattered and disjointed funding, inadequate genetic research facilities, ecological risk, inadequate skilled manpower and poor documentation of tilapia genetic resources among others. Considering the growing importance of tilapia culture, the need to document, conserve, evaluate and utilize tilapia genetic resources is highlighted to enhance the success of food security in Nigeria.
    Description: Includes:- 3 tables.;26 refs.
    Keywords: Aquaculture ; Oreochromis niloticus ; Nigeria ; Kainji L. ; freshwater environment ; Fish culture ; Genetics ; Selective breeding
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 23-28
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  • 3
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    FISON | Lagos (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/24202 | 19325 | 2018-05-16 15:26:01 | 24202 | Fisheries Society of Nigeria
    Publication Date: 2021-07-15
    Description: This paper, fish genetics and breeding in Nigeria addresses the application of genetics and breeding to fish farming with the view to enhanced aquaculture production in Nigeria. This production technique is not limited to the use of conventional breeding programs nor is it necessary to go through the basic developmental steps of selection and isolation that lead to the domestication and diversification of livestock and crops over thousands of years. With modern molecular genetic techniques and induced breeding, it is now possible to develop and create new fish species .
    Description: Includes: 12 references
    Keywords: Aquaculture ; Nigeria ; Delta State ; freshwater environment ; Genetics ; Sustainability ; Induced breeding ; Biotechnology ; Seed (aquaculture) ; Aquaculture techniques ; Fish culture
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 203-205
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  • 4
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    FISON | Minna (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/24085 | 19325 | 2018-05-12 14:21:06 | 24085 | Fisheries Society of Nigeria
    Publication Date: 2021-07-15
    Description: Application of genetic manipulation techniques on cultured fish have been employed in developed economies to increase food fish yield. In this study, a simple and safe biotechnology genetic manipulation technique was applied to produce triploid African giant catfish (Clarias gariepiirus Burchell 1822) larvae .Eggs numbering 100~c10 in quadruplicates from C. gariepinus were activated by milt from the same species and were subsequently transferred to a thermoregulated refrigerator maintained at 2degreesC for 20 min to suppress cell division, starting at 4 min after activation. Haploid larvae were produced by activating eggs with UV irradiated milt at 30000 uWcm-2 for 15 min. Fertility, hatchability and survival after one week for triploids were 82.5%, 69.8% and 61.3% against haploid and diploid controls, 100%, 15%, 0% and 100%,93%, 91 % (p 〈 0.05) respectively. Ploidy levels of the embryos cytogenetically were evaluated in day-old posthatched embryos incubated in 0.02% colchicine for 2-4 h. Head and yolk sac were discarded in 0.9% NaCl while remaining fragments were later treated with hypotonic solutions of distilled water for 5 min, and subsequently with diluted catfish serum 1: 4 for 25 min. Cells were fixed with 1:3 acetic acid ethanol mixture. Slide with the chromosome spreads were then stained with 20% Giemsa in phosphate buffered solution. Chromosome numbers obtained were 28~c2, 56~c2, and 75~c2 for haploid control, diploid control and triploid treatments respectively. Using a simple biotechnology technique as above, triploid C. gariepinus larvae were produced in the present study. The potential application on food fish security of triploid catfish in aquaculture especially in Nigeria is discussed.
    Description: Includes: 1 table and 1 plate.;Also includes: 20 refs.
    Keywords: Aquaculture ; Clarias gariepinus ; Nigeria ; Biotechnology ; Clarias gariepinus ; Chromosomes ; Aquaculture ; freshwater environment ; Genetics ; Cultured organisms ; Food fish ; Yield ; Biotechnology ; Fertility ; Hatching ; Sustainable development ; Aquaculture ; Aquaculture development ; Appropriate technology
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 117-122
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  • 5
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    FISON | Akure (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/23409 | 19325 | 2018-04-12 09:02:51 | 23409 | Fisheries Society of Nigeria
    Publication Date: 2021-07-14
    Description: A comparative analysis on biochemistry and Polyacryl Amide Gel Electrophresis was carried out to determine the genetic diversity of diploid and triploid Heterobranchus bidorsalis. Sixteen samples of diploid and triploid farm-raised (mean weight; 512.6g and mean length; 41.6cm) were collected and the electrophoresis analysis was conducted using 5.5% Polyacryl Amide Gel and serum protein obtained from the blood of the live samples. 0.06% Coomassie blue was used for staining the gel while a mixture of ratio 1:2 of glacial acetic acid, meethanol and distilled water was used for de-staining the gel. The diploid and triploid possessed an equal total number of 23 electrophoretic protein bands. The molecular phylogenetics of both samples revealed low genetic variability. Results of this study will serve as a baseline analysis on the current genetic diversity of H. bidorsalis in Nigeria.
    Description: Includes:- 2 figs.;8 refs.
    Keywords: Fisheries ; Heterobranchus bidorsalis ; Nigeria ; Kontagora L. ; freshwater environment ; Genetics ; Diploids ; Experimental culture ; Nutritional requirements ; Fish
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 168-169
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 225-242 
    ISSN: 0886-1544
    Keywords: spermatozoa ; calcium ion transport ; motility regulation ; cholinergic agonists ; ouabain ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Behavioral responses of mature spermatozoa treated with neurotropic factors suggest that calcium entry and intracellular transport may be regulated by a cholinergic mediated program. To test the validity of this proposed mechanism, the effect of several agents on Ca distribution in the sperm cell was examined cytochemically.Sites of Ca accumulation were visualized in thin sections of bull spermatozoa by the application of a modification of Gomori's histochemical procedure for phosphatases. Intact bull sperm cells incubated at room temperature in a buffered balanced salt solution containing 5 mM/liter of CaCl2 showed small, randomly scattered deposits of the reaction product. Similarly treated sperm cells, plasmolyzed in hypoosmotic KCl, revealed a greatly increased amount of deposit associated with the cell membranes (mitochondrial surfaces and plasmalemma), the axonemal complex components, and satellite fibers adjacent to the outer dense fibers. Preincubation of intact cells in nicotine or eserine considerably enhanced the entry of calcium into the cell and its association with the membranes and other intracellular organelles. Decamethonium, an irreversible depolarizer and blocker of cholinergic receptors, interfered with the uptake and intracellular distribution of the calcium. Ouabain, the digitalis glycoside that decreases progressive motility of bull sperm and inhibits Na-, K-ATPase, appears to block Ca efflux, causing an intense accumulation of electron-opaque particles in the plasma membrane while smaller numbers of particles are distributed sparsely throughout the cell interior.The cytochemical results showing enhanced calcium entry in the presence of cholinergic agents, depressed intracellular calcium in cells treated with cholinergic receptor blocker, and intense accumulation of calcium within the cell membrane in the presence of ouabain are consistent with spermatozoan behavioral responses to these agents. These observations support the concept that neurotropic factors may be involved in regulating transmembrane and intracellular transport of ions in control of sperm cell function.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 309-315 
    ISSN: 0886-1544
    Keywords: tropomyosin ; avian muscular dystrophy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The isotype pattern of tropomyosin was investigated in normal and dystrophic avian pectroal muscle using two-dimensional gel electrophoresis. Previous reports have shown that adult pectoral muscle of chickens contains only the α-subunit of tropomyosin and a breast-type troponin-T (TN-T), whereas pectoral fetal muscle contains both α- and β-tropomyosin and leg-type TN-T. The change from the fetal to the adult forms begins shortly after hatching. It has been previously reported that avian dystrophic pectoral muscle contains both the leg- and breast-type TN-T; we show that in avian dystrophic muscle there is also persistent expression of the β-subunit of tropomyosin.
    Additional Material: 4 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982) 
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 287-308 
    ISSN: 0886-1544
    Keywords: actin-binding protein ; Dictyostelium ; cytoskeleton ; amoeboid movement ; calcium ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A protein from Dictyostelium discoideum with an apparent subunit molecular weight of 95,000 daltons (95K protein) was previously identified as an actin-binding protein ‘Hellewell and Taylor, 1979’. In this paper, we present a method for purifying the protein, and characterize some important aspects of its structure and function. Purification of the 95K protein is achieved by fractionation with ammonium sulfate followed by chromatography on DEAE-cellulose, gel filtration on 6% agarose, and final purification on hydroxyapatite. The 95K protein is a dimer, composed of apparently identical subunits. It is a rod-shaped molecule, 38 nm in length, with a Stokes radius of 74 Å. In these structural properties, the 95K protein is similar to muscle and nonmuscle α-actinins. The 95K protein and filamin are equally competent, when compared on a weight basis, to enhance the apparent viscosity of actin as determined by falling ball viscometry. The apparent viscosity of mixtures of the 95K protein and actin is dramatically reduced at pH greater than 7.0 or free ‘Ca2+’ greater than 10-7 M. We also examine the mechanism by which calcium regulates the interaction of the 95K protein and actin. A change in free ‘Ca2+’ induces no detectable change in the quaternary structure of the 95K protein. Our experiments indicate that the 95K protein does not dramatically alter the length distribution of actin filaments in the presence of micromolar free ‘Ca2+’. A large fraction of the 95K protein cosediments with actin in the presence of low free ‘Ca2+’ (ca. 3 × 10-8M), but not in the presence of high free ‘Ca2+’ (ca. 4 × 10-6M). We conclude that increased free ‘Ca2+’ inhibits gelation of actin by the 95K protein by reducing the affinity of the 95K protein for actin. We propose that 95K protein is an important component of the cytoskeletal/contractile system in D. discoideum amoebae.
    Additional Material: 14 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 343-354 
    ISSN: 0886-1544
    Keywords: NBD-phallacidin ; actin ; ocular tissues ; wound repair ; stress fibers ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The fluorescent derivative of the actin-binding toxin phallacidin, 7-nitrobenz-2-oxa-1,3 diazole phallacidin, has been used to cytologically demonstrate the presence of actin in lens epithelium, corneal endothelium, and retinal pigment epithelium. In these noninjured tissues, no stress fibers are observed and fluorescence is confined mainly to an area at or near the cell membrane, although some diffuse cytoplasmic staining can also be seen. However, following injury to either the lens epithelium or corneal endothelium of rats and frogs, stress fibers are detected, but only in those cells that migrate into the wound area. Cells on the periphery of each tissue do not partake in would repair and thus maintain their normal appearance. After the tissue has regenerated, stress fibers disappear, and those cells involved in the injury response return to their normal morphology.When rabbit corneal endothelium is placed in tissue culture, stress fibers are observed as the cells migrate away from the initial explant. Upon reaching confluency, these cells spread out and each is surrounded by thick actin-containing bands. Furthermore, they exhibit some stress cables within their cytoplasm. This is in contrast to their appearance in vivo where stress fibers are absent and fluorescence is limited to a region near the cell membrane.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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