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  • Genetics  (2,233)
  • Wiley-Blackwell  (2,228)
  • FISON  (5)
  • Institute of Physics
  • PANGAEA
  • Periodicals Archive Online (PAO)
  • 1
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    FISON | Lagos (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/24202 | 19325 | 2018-05-16 15:26:01 | 24202 | Fisheries Society of Nigeria
    Publication Date: 2021-07-15
    Description: This paper, fish genetics and breeding in Nigeria addresses the application of genetics and breeding to fish farming with the view to enhanced aquaculture production in Nigeria. This production technique is not limited to the use of conventional breeding programs nor is it necessary to go through the basic developmental steps of selection and isolation that lead to the domestication and diversification of livestock and crops over thousands of years. With modern molecular genetic techniques and induced breeding, it is now possible to develop and create new fish species .
    Description: Includes: 12 references
    Keywords: Aquaculture ; Nigeria ; Delta State ; freshwater environment ; Genetics ; Sustainability ; Induced breeding ; Biotechnology ; Seed (aquaculture) ; Aquaculture techniques ; Fish culture
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 203-205
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  • 2
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    FISON | Minna (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/24085 | 19325 | 2018-05-12 14:21:06 | 24085 | Fisheries Society of Nigeria
    Publication Date: 2021-07-15
    Description: Application of genetic manipulation techniques on cultured fish have been employed in developed economies to increase food fish yield. In this study, a simple and safe biotechnology genetic manipulation technique was applied to produce triploid African giant catfish (Clarias gariepiirus Burchell 1822) larvae .Eggs numbering 100~c10 in quadruplicates from C. gariepinus were activated by milt from the same species and were subsequently transferred to a thermoregulated refrigerator maintained at 2degreesC for 20 min to suppress cell division, starting at 4 min after activation. Haploid larvae were produced by activating eggs with UV irradiated milt at 30000 uWcm-2 for 15 min. Fertility, hatchability and survival after one week for triploids were 82.5%, 69.8% and 61.3% against haploid and diploid controls, 100%, 15%, 0% and 100%,93%, 91 % (p 〈 0.05) respectively. Ploidy levels of the embryos cytogenetically were evaluated in day-old posthatched embryos incubated in 0.02% colchicine for 2-4 h. Head and yolk sac were discarded in 0.9% NaCl while remaining fragments were later treated with hypotonic solutions of distilled water for 5 min, and subsequently with diluted catfish serum 1: 4 for 25 min. Cells were fixed with 1:3 acetic acid ethanol mixture. Slide with the chromosome spreads were then stained with 20% Giemsa in phosphate buffered solution. Chromosome numbers obtained were 28~c2, 56~c2, and 75~c2 for haploid control, diploid control and triploid treatments respectively. Using a simple biotechnology technique as above, triploid C. gariepinus larvae were produced in the present study. The potential application on food fish security of triploid catfish in aquaculture especially in Nigeria is discussed.
    Description: Includes: 1 table and 1 plate.;Also includes: 20 refs.
    Keywords: Aquaculture ; Clarias gariepinus ; Nigeria ; Biotechnology ; Clarias gariepinus ; Chromosomes ; Aquaculture ; freshwater environment ; Genetics ; Cultured organisms ; Food fish ; Yield ; Biotechnology ; Fertility ; Hatching ; Sustainable development ; Aquaculture ; Aquaculture development ; Appropriate technology
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 117-122
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  • 3
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    FISON | Akure (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/23373 | 19325 | 2018-04-10 16:09:38 | 23373 | Fisheries Society of Nigeria
    Publication Date: 2021-07-14
    Description: The contribution of tilapia aquaculture in Nigeria to world output is negligible due to stunting, poor market value among others. This paper evaluates the aquacultural credentials of tilapia, some genetic improvement technology in cultured tilapia, namely, ploidy, hormonal sex reversal. transgenic, hybridization, and the necessity of Genetic Improvement in accelerating tilapia production in Nigeria. Investigation reveals the presence of O. niloticus with the highest growth perfermancc index (~h1=3.11) for Lake Kanji which indicates high growth potential in suitable culture environment and could serve as a good starting point for genetic development. The presence of ”wesafu”, an ecotype cichlid, endemic to Epe lagoon, Lagos, which grows to 1500g in the wild, appears to be an excellent candidate for genetic improvement of a commercial strain for the growing aquaculture industry. Tilapia Genetic improvement in Nigeria is faced with a number of setbacks. This includes short- term, scattered and disjointed funding, inadequate genetic research facilities, ecological risk, inadequate skilled manpower and poor documentation of tilapia genetic resources among others. Considering the growing importance of tilapia culture, the need to document, conserve, evaluate and utilize tilapia genetic resources is highlighted to enhance the success of food security in Nigeria.
    Description: Includes:- 3 tables.;26 refs.
    Keywords: Aquaculture ; Oreochromis niloticus ; Nigeria ; Kainji L. ; freshwater environment ; Fish culture ; Genetics ; Selective breeding
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 23-28
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  • 4
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    FISON | Akure (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/23409 | 19325 | 2018-04-12 09:02:51 | 23409 | Fisheries Society of Nigeria
    Publication Date: 2021-07-14
    Description: A comparative analysis on biochemistry and Polyacryl Amide Gel Electrophresis was carried out to determine the genetic diversity of diploid and triploid Heterobranchus bidorsalis. Sixteen samples of diploid and triploid farm-raised (mean weight; 512.6g and mean length; 41.6cm) were collected and the electrophoresis analysis was conducted using 5.5% Polyacryl Amide Gel and serum protein obtained from the blood of the live samples. 0.06% Coomassie blue was used for staining the gel while a mixture of ratio 1:2 of glacial acetic acid, meethanol and distilled water was used for de-staining the gel. The diploid and triploid possessed an equal total number of 23 electrophoretic protein bands. The molecular phylogenetics of both samples revealed low genetic variability. Results of this study will serve as a baseline analysis on the current genetic diversity of H. bidorsalis in Nigeria.
    Description: Includes:- 2 figs.;8 refs.
    Keywords: Fisheries ; Heterobranchus bidorsalis ; Nigeria ; Kontagora L. ; freshwater environment ; Genetics ; Diploids ; Experimental culture ; Nutritional requirements ; Fish
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 168-169
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  • 5
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    FISON | Lagos, Nigeria
    In:  http://aquaticcommons.org/id/eprint/23241 | 19325 | 2018-03-18 15:26:11 | 23241 | Fisheries Society of Nigeria
    Publication Date: 2021-07-13
    Description: Cephalic abnormality in nine mating groups involving Clarias gariepinus with cephalic abnormality, non Cephalic and Maiduguri origin were investigated to determine the level and the genetic basis of the occurrences and its aquaculture implication. The highest mean percentage survival in a Cephalic crossed with non cephalic group was 68.6% whereas the last mean percentage survival was 25.7% in the group of Maiduguri cross with Maiduguri parent. Cephalic abnormality was observed in the crosses with both female and male cephalic with a total frequency ranging from 0 % to 70.8. %. The least number of cephalic abnormality was 18.5% which involved a cross of non cephalic with cephalic, and the highest was 70.8%. This level of cephalic abnormality shows that the defect was hereditary. This result implies that, the genetic factor is a major contributor in the feature of cephalic abnormality in Clarias gariepinus.
    Description: Includes:-1 table.;3 appendixes.;11 refs.
    Keywords: Aquaculture ; Fisheries ; Clarias gariepinus ; Nigeria ; freshwater environment ; Diseases ; Deformation ; Fish culture ; Genetics ; Abnormalities ; Genetic abnormalities
    Repository Name: AquaDocs
    Type: conference_item , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 199 - 208
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 27 (1990), S. 168-172 
    ISSN: 1040-452X
    Keywords: Motility ; Genetics ; Sex chromosome ratio ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In this study, we address the relationship between motility and genetic content of mouse sperm. The chromosome complements of highly motile mouse sperm, selected using the swim-up technique, were analyzed after in vitro fertilization, at the first cleavage state. They were compared to those of unselected sperm. Identification of male and female chromosome sets was possible because of their differential condensation at the first mitotic division. In vitro fertilization, swim-up separation, chromosome preparation, and staining were carried out using standard techniques. The results indicate that highly motile mouse sperm did not differ in types and frequencies of chromosomal abnormalities from those not selected for motility. Moreover, separation of motile sperm does not deviate the sex ratio from the theoretical 1:1.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 186-196 
    ISSN: 0173-0835
    Keywords: Genetics ; Two-dimensional electrophoresis ; Denaturing gradient electrophoresis ; Cystic fibrosis ; Mutation ; Breast cancer ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A major effort in the analysis of DNA currently focuses on identifying genes and their pathological variants underlying disease. Once such disease genes have been isolated a major task of molecular medicine is to identify the spectrum of DNA sequence variations responsible for the aberrant function of such genes. These efforts, however, are hindered by the vast amount of genetic information to scan for variations and the limited capacity of analytical techniques in terms of accuracy and speed. Recently, a number of techniques were developed, so-called “genome scanning” techniques, which allow complete genomes to be analyzed for sequence variation in parallel, i.e., at multiple sites or loci simultaneously rather than serially at predefined loci. Here we present the background and applications of a particular electrophoretic parallel processing approach, generically termed two-dimensional DNA typing. The approach is based on separating DNA fragments by two-dimensional electrophoresis [1], including denaturing gradient gel electrophoresis, thus allowing hundreds of fragments to be simultaneously assessed by comparative analysis for variations in size and sequence. The method is suitable for hybridization analysis with locus-specific and multilocus probes of genomic DNA restriction fragments derived from human and other DNA, and for analysis of polymerase chain reaction (PCR) fragments derived from large genes. Two-dimensional DNA typing has been applied, e.g., in linkage analysis of pedigrees, analysis of tumor genomes for rearrangements, and to scan the cystic fibrosis transmembrane regulator gene for sequence variations such as point mutations.
    Additional Material: 3 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 1 (1979), S. 1-1 
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 1 (1979), S. 47-60 
    ISSN: 0192-253X
    Keywords: developmental mutants of Physarum ; apogamic mutants ; the amoebal-plasmodial transition ; myxomycete genetics ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In the heterothallic myxomycete Physarum polycephalum, uninucleate amoebae normally differentiate into syncytial plasmodia following heterotypic mating. In order to study the genetic control of this developmental process, mutations affecting the amoebal-plasmodial transition have been sought. Numerous mutants characterized by self-fertility have been isolated. The use of alkylating mutagens increases the mutant frequency over the spontaneous level but does not alter the mutant spectrum. Three spontaneous and 14 induced mutants have been analyzed genetically. In each, the mutation appears to be linked to the mating type locus. In three randomly selected mutants, the nuclear DNA content is the same in amoebae and plasmodia, indicating that amoebal syngamy does not precede plasmodium development in these strains. These results indicate that a highly specific type of mutational event, occurring close to or within the mating type locus, can abolish the requirement for syngamy normally associated with plasmodial differentiation. These mutations help define a genomic region regulating the switch from amoebal to plasmodial growth.
    Additional Material: 1 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 1 (1979), S. 77-95 
    ISSN: 0192-253X
    Keywords: Drosophila melanogaster ; pupae ; heat shock ; protein synthesis ; phenocopies ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Pupae of Drosophila melanogaster were heat-shocked under conditions required to induce phenocopies in more than 90% of the flies that subsequently emerge. The effects of these treatments on protein synthesis in two tissues (thoracic epithelium and brain) were followed for several hours after the heat treatments. Results from pulse-labeling and protein separations on sodium dodecylsulfate (SDS) acrylamide gels showed a virtually complete cessation of protein synthesis immediately after the shock, followed by a noncoordinate resumption of the starting pattern. Similar experiments following double heat shocks demonstrated a more rapid resumption of synthesis of heat shock proteins after two successive heat treatments than after a single one.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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