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  • Articles  (36)
  • Fertilization
  • Tehran, Iran  (35)
  • Cell Press  (1)
  • Molecular Diversity Preservation International (MDPI)
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  • Articles  (36)
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  • 1
    Publication Date: 2021-05-19
    Description: This study was conducted in the Persian Gulf (Coastal waters of Khuzestan province) from March 2009 to April 2010. The 237 specimen of Silver pomfert P. argenteus collected. Maximum of mature brooders at Jun and maximum of hatched eggs obtained at same month. Artificial fertilization was successful. Absolut fecundity was between 19000 to 38000.maximum of hatch was 51 percent. Maximum of fertilization was 32 percent and continued development of larvae to 35 days. Average of fertilized eggs was 1.1 mm. newly hatched larve were 2.2 to 2.4 mm. The all Silver Pomfret larve were dead at prude 35 days gently. Suppose n enhancement phase wasn't performed.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Artificial reproduction ; Pampus argentus ; Larvae rearing ; Eggs hatch ; Specimen ; Silver pomfert ; P.argenteus ; Mature ; Brooders ; Artificial fertilization ; Fecundity ; Fertilization
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 57pp.
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  • 2
    Publication Date: 2021-05-19
    Description: Considering decrease in total catch of sturgeon & threat of extinction in their stocks, special measures might be adopted. Sperm cryopreservation is one of the suitable methods to prepare bank of frozen gamete for future use in artificial breeding in order to prohibit extinction of sturgeon stocks. This study carried out on 27 male sturgeon during 2001-2004. The investigated sturgeon include 12 male Persian sturgeon (Acipenser persicus), 7 male Acipenser stellatus, 5 male Acipenser nudiventris and 3 male Huso huso. Sperm Collected from spawners in Shahid Beheshti & Shahid Marjani sturgeon rearing & propagation Complex in Rasht & Gorgan. The Sperm which was collected from Shahid Marjani propagation complex placed in the sealed Vessels & transferred by coleman in near zero temperature to cryopreservation laboratory of international sturgeon research institute for further investigation. In this study, the sperm was diluted in ratio 1:1 in two culture media containing dimethyl sulfoxide & glycerol (BC) and the samples stored in 1 ml insulin syringe & 0.5 ml Piot. The diluted sperm froze in a special temperature by automated freezer model 5300 (France IMV). Three phases applied to freeze the samples which are as follow: 1- Begin to freeze from +50c to -100c (30c /min) 2- From 10 0c to 70 0c (20 0c /min) 3- From 70 0c to 130 0c (25 0c /min) After freezing, the samples placed in liquid nitrogen containers with 196 0c temperature. For thawing, the sperm samples took out of liquid nitrogen & placed in water 40 0c. Then motility percent & the sperm quality investigated under 400x microscope. According to the results the mean motility percentage of fresh sperm in Persian sturgeon, Acipenser Stellatus, Acipenser nudiventris and Huso huso was 84, 73.75, 67.5 and 76.66, respectively. The mean percentage of motility in frozen sperm which placed in media containing dimethyl sulfoxide was 32, 37.5, 40 and 20%, respectively. Also, the frozen samples that preserved in BC media (Biociphus) showed 5.2, 75.25, 4.11 and 2.66% motility, respectively. In blank group, the mean fertilization percent of eggs was 90, 72, 71.25 and 90%, respectively. In the treatment group applying frozen sperm in culture media containing dimetlylsulfoxide, the mean fertilization rate was 30, 6.5, 25.39 and 4.75%. Furthermore, no fertilization (0.0%) observed using frozen sperm stored in Biociphus culture media containing glycerol. There was no significant difference in fertilization percent comparing the two storage places (syringe & Piot). According to investigations the culture media containing dimethyl sulfoxide, is a suitable diluter for sturgeon sperm. So, the Cryopreservation technique can be used to preserve the sturgeon sperm for future fertilization & through this way we can prevent extinction of sturgeon stocks.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Cryopreservation ; Sperm ; Acipenser nudiventris ; Huso huso ; Acipenser stellatus ; Acipenser persicus ; Breeding ; Sturgeon ; Temperature ; Motility ; Fertilization ; Spermatozoa ; Freezing
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 53pp.
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  • 3
    Publication Date: 2021-05-19
    Description: Barbus sharpeyi is a local fish of Khouzestan that is planned to be a target for aquaculture in recent programs of fisheries organization. Considering importance of this species in its dispersal region, the main goal of this project was evaluation of possibility for triploidy induction and its potential in response to the heat shocks, efficiency of viability and growth and finally reporting the best condition for triploidy induction in Benny. Induction of thermal shocks was executed in for cold and heat shocks (2 and 4°C for cold and 34, 36 and 38°C for heat). Time of induction and its duration varied between 2 and 5 minutes after the fertilization for 3 and 5 minutes. Each treatment was repeated for 3 times. The ploidy level was determined based on size of nucleus diameters in erythrocytes. Analysis of data was done by SPSS (ver. 16) using T-test and ANOVA method. Results showed that the maximum number of triploid individuals was obtained in treatment of 38°C, 2 min after the fertilization by duration of 3 minutes but as the condition was not suitable for the viability of the eggs, losses of the larvae was high in this group. The best efficiency of triploidization in B. sharpeyi belongs to the 34°C, 2 to 5 minutes after the fertilization for duration of 5 minutes. Nuclear dimensions showed an increase in triploids and confirmed that this characteristic can be used as a reliable factor to distinguish polyploidy. Results of this study showed that B. sharpeyi has the ability for polyploidy inductions specially heat shocks. Evaluation of growth in matured fishes, use of proper tagging systems to distinguish the treatments and designing a plan for bioconserving and genetic improvement of this species is recommended.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Aquaculture ; Triploidy ; Benni ; Barbus sharpeyi ; Thermal shocks ; Species ; Growth ; Fertilization ; ANOVA ; Bio conserving ; Genetic
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 72pp.
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  • 4
    Publication Date: 2021-05-19
    Description: In order to study phytopague fingerlings feeding in fish ponds, simultaneously to the beginning of warm season and cultivation, fingerlings of 3-5 gr from 4 ponds in Mazandaran province were captured, fixed with formalin, then transferred to the plankton determination laboratory of Caspian sea ecologic institute. Based on carried out studies on the first and the end party of their intestine and the pond water, phytophague fingerlings feeds on phytoplankton, zooplankton and detritus. But the essential nutrition was on phytoplankton and detritus. Planktonic groups were formed of Cyanophyta, Chlorophyta, Chrysophyta and Euglenophyta. But in addition to these four groups Pyrophyta was present in fish pond water. Relative abundance of these nutrient organisms in water and intestine was different. Also in most cases a direct relation was observed between the intensity of water organisms and intestine ingredients. Based on this study, essential differences of digestion were observed following to the source of nutrition and the most difference were considered in digestion of Chrysophytes, Cyanophyta and Chlorophyta. Base on the results of this study we propose to make more studies on fertilization and the proportion of different fertilizers in fish ponds in order to obtain easily digestible phytoplankton thus increase in fish exploitation.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Silver carp fry ; Nutrition ; Phytoplanktons ; Zooplanktons ; Chrysophyta ; Cyanophyta ; Clorophyta ; Pyrophyta ; Euglenophyta ; Feeding ; Hypophthalmichthys molitrix ; Fingerlings ; Fertilization
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 83pp.
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: In this work we have surveyed on six ponds of p.semisulcatus shrimp in the investigation farn1 that have located at helleh site in the Bushehr province from May 2001. Our main goal in this project were : (l) Measurements of quality factors in water and sediment (2) Offering possible proposals for making development in water quality of culture ponds With respect to the time of shrimp stocking and harvesting the frequencies of water and sediment sampling were twice and once per month , respectively. Also two sediment sampling of the pond bed have done before pond watering and after harvesting The measured factors in the water were : pH , salinity , dissolved oxygen , transparency , temperature ( Twice a, day in the morning and the afternoon ) , chlorophyll-a nitrate , nitrite orthophosphate , iron , biochemical oxygen demand , chemical oxygen demand , TSS , T DS, total alkalinity, organic nitrogen the sediment were : pH , alkalinity , iron , total organic matter (TOM) , total nitrogen and grain size The measurement of grain size have done in two times ( before pond watering and after harvesting The results show low concentration of the essential nutrient such as phosphate and nitrate , high concentration of ammonia , high pH , undesirable transparency , high alkalinity of sediments and a great amount of clay in pond bed On the basis of above description , we need to renewing our management in fertilizing , feedings , liming and important the quality of bed.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Survey ; Penaeus semisulcatus ; Dissolved oxygen ; Transparency ; Temperature ; Chlorophyll-a ; Alkalinity ; Fertilization ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 69pp.
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  • 6
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: This study has been carried out to determine the possibility of producing Gattan fish (Barbus xanthopterus) brooders in captivity condition. For this reason during in year 2004-2005, 600 specimen of Gattan in three pond and in 2008, from two local point shilat and research center, 200 specimen in two ponds were placed. The area of each pond was 1800 square meters. In the first period of rearing, total weight and total length of nearly two years old brooders reached to 989 g and 44 cm respectively. All of the fishes were male (except one case) and they had become mature. In second rearing period, 27 percent of shilat specimens and 42 percent of research center specimens were female. In 22 months old fishes, the males and females of shilat specimens reached to mean weight 716 and 658 g and mean length 382 and 365 cm respectively. Also, the male and females of research center specimens reached to mean weight was 1195 and 11064 g and mean length 443 and 425 cm respectively, when they were 22 months old. The absolute and specific growth rate in two rearing periods showed ascending trend and the 100 percent of brooders were survived. The male fishes were matured in 2 years old and the mature female fishes were not observed. The brooders response to pituitary extract in research center specimens was found to be more than shilat specimens.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Rearing ; Gattan ; Maturity ; Sex ratio ; Fertilization ; Hatching ; Fecundity ; Survivor ; Brood production ; Barbus xanthopterus ; Fingerling ; Specimen
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 38pp.
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  • 7
    Publication Date: 2021-05-19
    Description: This study was aimed to investigate the effects of different doses of two hormones and an anti-aromatase, i.e. 17 methyl testosterone (MT), methyl di hydrotestosterone (MDHT) or mestanolone and letozole in masculinization of Nile tilapia (Oreochromis niloticus) under the condition of brackish water in Bafgh station situated in Yazd province in center of Iran. Each experiment in this study was consisted of 5 treaments with 3 replicates each. A number of 1725 larvaes was distributed randomly among 15 replicates at the beginning of each experiment. Each experiment lasted 45 days and the larvaes were reared in aerated flow-through pots and fiberglass tanks filled with brackish water. The averages for temperature, salinity, pH and dissolved oxygen of water were 26.9 ê, 8 g/l, 7.6 and 5.78% respectively during this study. In experiment 1, three different doses of 40, 60 and 100 mg MT/k of feed were fed to different groups of 7 day post fertilization (dpf) larvaes for 45 days from the beginning of the experiment. The results showed that the larvaes in 40 mg group were 100 percent masculinized based on squash test performed at the end of the experiment but masculinization rates of those in 60 and 100 mg groups were 99.7 and 96.2 perecent respectively. Based on Dunkan test, total body length and weight averages measured in biometry 3 (at the end of the experiment) were not significantly different among groups but in biometry 2 (30 days after the beginning of experiment), they were significantly lesser only in 40 mg group (P〈0.05). There was significant differences in survival rate of different groups of larvaes in this experiment based on chi-square test (χ=31.166, P〈0.05) and the values in 40 and 100 mg groups (74.5 and 82.9% respectively) were lesser than those in 60 mg, control 1 and control 2 groups (84.3, 89.0 and 87.0 respectively). In experiment 2, masculinization rates of two different groups of larvaes immersed in 1800 µg MDHT/liter once in 10dpf and twice in 10 and 14dpf were 80.0 and 91.9 percent respectively. There were no significant differences in total body length and weight averages measured in biometry 2 between different groups but significant differences were observed in total body length only in biometry 3 (P〈0.05) where the highest values occurred in experiment 1 and control 1 groups and the lowest one in experiment 2. Significant differences observed in survival rate of different groups of larvaes in this experiment based on chi-square test (χ=15.165, P〈0.05) and the rates in experiment 1, control 2 and 3 groups (89.9, 86.4 and 89.9% respectively) were higher than those in experiment 2 and control 1 groups (82.0 and 82.3 respectively). In experiment 3, three different doses of anti-aromatse letrozole (200, 300 and 400 mg/k feed) were fed to different groups of 7 day post fertilization (dpf) larvaes for 45 days from the beginning of the experiment. The larvaes in 400 group .were all masculinized whereas the masculinization rates in 200 and 300 mg groups were 99.0 and 99.6% respectively. There were significant differences in total body length and weight averages measured in biometry 2 and 3 among groups in this experiment (P〈0.05) where the highest and the lowest values occurred in control 2 and experime2 groups respectively. Based on chi-square, the survival rate of different groups was significantly different (χ=41.119, P〈0.05) and the lowest rate occurred in experiment 2 group. No significant differences observed in gender ratios whithin all control groups in this study based on chi-square test. According to the findings aquired under the condition of brackish water at the present study, it would be potentially recommended to use MT and letrozole for the production of all male polpulations of Nile tilapia fish in order to provide fish farmers with no harmful environmental impacts on water sources in rivers and seas which occured due to the uncontroled breeding of tilapia. However, more research is needed to draw firm conclusions to use hormones and in especial anti-aromase letrozole because of the shortage of sufficient data in current references.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Masculinization ; Nile tilapia ; 17α-methyl testosterone ; Methyl di hydro testosterone ; Mestanolone ; Body weight gain ; Total body lenght ; Brackish water ; Male ; Oreochromis niloticus ; Hormones ; Temperature ; Salinity ; pH ; Dissolved oxygen ; Fertilization ; Survival rate ; Larvae ; Investigation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 61pp.
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  • 8
    Publication Date: 2021-05-19
    Description: breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sperm cryopreservation ; Rainbow trout ; Extender ; Cryoprotectant ; Onchorhynchus mykiss ; Breeding ; Genetic ; Coldwater ; Temperature ; Fertilization
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 51pp.
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  • 9
    Publication Date: 2021-05-19
    Description: Sturgeon stocks are drastically decreasing in Caspian Sea during recent decades. This project has been proposed to collect the excess sperm of Caspian Sea Southern part sturgeon breeders, to cryopreserve and hold them for further use. During two spawning seasons (2006-2007), sperm quality and quantity analysis were conducted on 34 different male sturgeons of which 12 Persian sturgeon, 4 stellate sturgeon, 1 beluga and 1 ship sturgeon with better quality were chosen to cryopreserve. After dilution of fresh sperm with sturgeon sperm diluent (115 mM Tris, 23.4 mM sucrose and 15% DMSO) at the ratio of 1:1, the 0.5 ml straws were filled using special filling and sealing apparatus and frozen with a multi step freezing rate. To access the further quality and fertilization test, samples were thawed at 40 °C for 20 seconds. During the project performance, 1010 ml of Persian sturgeon sperm , 110 ml of ship sturgeon sperm , 90 ml of stellate and 80 ml of beluga sperm were cryopreserved. The reduction of frozen-thawed sperm motility in comparison with fresh samples showed 10-52.3 % decrease, but there was not any significant differences between samples after 7 months preservation in liquid Nitrogen (P 〈 0.005). River caught Persian sturgeon samples showed higher sperm density in comparison with nonriverine breeders (P 〈 0.05). Data showed that stellate sturgeon sperm showed better resistance to freezing condition in comparison with Persian sturgeon spermatozoa. Fertilization tests (4 hours after fertilization)showed 27.4% and 58.2% fertilization rate in frozen-thawed stellate and Persian sturgeon spermatozoa, respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Spermatozoa ; Cryopreservation ; Percentage of motility ; Sperm bank ; Sturgeon ; Brood stocks ; Spawning ; Fertilization ; Samples ; Density
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 50pp.
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  • 10
    Publication Date: 2021-05-19
    Description: The recirculation of inorganic nutrients orthophosphate and nitrate in relation with productivity in sturgeon rearing ponds was studied through two rearing periods at the Shahid Beheshti Hatchery. The first rearing period commenced on May 24, 1998 and ended on July 26, 1998 whereas the second rearing period commenced on May 16, 1999 and ended on June 26, 1999. Four earthen ponds with a surface area of 2 hectares each were used in this studied of which three ponds (number 25, 26 and 27) were considered experimental ponds and one pond (number 24) was used as the control. Results obtained in the first rearing period indicate that the fertilization program employed in this study was suitable to maintain the productivity of experimental ponds. Growth rates in fingerlings also indicate a rapid increase in growth during the first few weeks of rearing. However unfavorable conditions encountered at the end of the rearing period (increase in water temperature, decrease in dissolved oxygen concentrations, deterioration of water quality etc.,) lead to decrease in food consumption by fingerlings that ultimately resulted in decrease in growth and survival rate of fingerlings (survival rate 60 % in first year). On the basis of the results obtained in the first year, measures were taken to and increase the biomass of benthic organisms in the rearing ponds. The biomass of benthic organisms in experimental ponds increased from a maximum of 2.1 g m-2 in the first year to a maximum of 189 g mo in the second year. However despite the management policies employed to maintain productivity and meet the food requirements of A. persicus fingerlings, water scarcity that prevailed in the entire region lead to an increase in water temperature, decrease in dissolved oxygen concentrations, over growth of aquatic vegetation and thus survival rate of fingerlings dropped to less than 40 % in the second year. Results obtained from scientific studies reveal that increase in pond productivity does not depend on basic knowledge alone. Techniques involved in pond management that provide suitable conditions required for fish culture such as stock assessment* basic control of fish growth, proper utilization of food resources in ponds and fertilization etc., play a significant role in this regard.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sturgeon culture ; Productivity ; Fertilization ; Live food ; Survival ; Nutrients
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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