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  • Biophysics
  • Cell Press  (2)
  • Springer Nature  (2)
  • Institute of Electrical and Electronics Engineers (IEEE)
  • Springer Science + Business Media
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  • 1
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    Novel Plant Imaging and Analysis (2021)
    Springer Nature | Springer Singapore
    Details
    Publication Date: 2024-04-05
    Description: This open access book is only an introduction to show that radiation and radioisotopes (RI) are premier tools to study living plant physiology which leads to new findings. Who had ever imagined that we could see water in a plant? Who had ever imagined that we could see ions moving toward roots in solution? Who had ever imagined that we could see invisible gas (CO2) fixation and movement in a plant? These studies demonstrated for the first time that water, ions and gas can be visualized in living plants, which could be hardly seen by anyone before. This publication summarizes the results obtained by Nakanishi’s lab in The Univ. of Tokyo, based on her original concept and her original tools or systems. It is useful for professional scientists, plant physiologist, and those studying plant imaging. The chapters demonstrates the innovative imaging work of the author, using radioactive tracers and neutron beam to follow the absorption and transport manner of water as well as major, minor, and trace elements in plants. Through these studies the author developed a real-time macroscopic and microscopic imaging system able to apply commercially available gamma- and beta-ray emitters. The real-time movement of the elements is now possible by using 14C, 18F, 22Na, 28Mg, 32P, 33P, 35S, 42K, 45Ca, 48V, 54Mn, 55Fe, 59Fe, 65Zn, 86Rb, 109Cd, and 137Cs. The imaging methods was applied to study the effect of 137Cs following 3/11 Fukushima Daiichi nuclear plant accident, which has revealed the movements of radiocesium in the contaminated sites.
    Keywords: Plant Physiology ; Biological and Medical Physics, Biophysics ; Imaging / Radiology ; Nuclear Chemistry ; Spectroscopy/Spectrometry ; Materials Science, general ; Bioanalysis and Bioimaging ; Radiology ; Spectroscopy ; Imaging Techniques ; Open Access ; Living plant activity ; Water imaging ; Element movement ; Visualization of gas fixation ; Fixed carbon movement ; Neutron beam imaging ; Radioisotope imaging ; RI ; 32P ; 33P ; Botany & plant sciences ; Biophysics ; Medical physics ; Medical imaging ; Nuclear chemistry, photochemistry & radiation ; Spectrum analysis, spectrochemistry, mass spectrometry ; Materials science ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences ; thema EDItEUR::P Mathematics and Science::PH Physics::PHV Applied physics::PHVN Biophysics ; thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKS Medical imaging::MKSH Medical imaging: radiology ; thema EDItEUR::P Mathematics and Science::PN Chemistry::PNR Physical chemistry::PNRL Nuclear chemistry, photochemistry and radiation ; thema EDItEUR::P Mathematics and Science::PN Chemistry::PNF Analytical chemistry::PNFS Spectrum analysis, spectrochemistry, mass spectrometry ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TG Mechanical engineering and materials::TGM Materials science
    Language: English
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  • 2
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    Low-Dose Radiation Effects on Animals and Ecosystems (2021)
    Springer Nature
    Details
    Publication Date: 2024-03-31
    Description: This open access book summarizes the latest scientific findings regarding the biological effects of the Fukushima Daiichi Nuclear Power Plant (FNPP) accident in 2011. Various cases of changes in animals and organisms have been reported since the FNPP accident. However, it is often unknown whether they are actually due to radiation, since the dose or dose-rate are not necessarily associated with the changes observed. This book brings together the works of radiation biologists and ecologists to provide reliable radioecology data and gives insight into future radioprotection. The book examines the environmental pollution and radiation exposure, and contains valuable data from abandoned livestock in the ex-evacuation zone and from wild animals including invertebrates and vertebrates, aqueous and terrestrial animals, and plants that are subjected to long-term exposure in the area still affected by radiation. It also analyzes dose evaluation, and offers new perspectives gained from the accident, as well as an overview for future studies to promote radioprotection of humans and the ecosystem. Since the biological impact of radiation is influenced by various factors, it is difficult to scientifically define the effects of low-dose/low-dose-rate radiation. However, the detailed research data presented can be combined with the latest scientific and technological advances, such as artificial intelligence, to provide new insights in the future. This book is a unique and valuable resource for researchers, professionals and anyone interested in the impact of exposure to radiation or contamination with radioactive materials.
    Keywords: Life sciences ; Animal genetics ; Radiation protection ; Radiation—Safety measures ; Cancer research ; Radiology ; Human physiology ; Biophysics ; Biological physics ; thema EDItEUR::M Medicine and Nursing::MF Pre-clinical medicine: basic sciences::MFG Physiology ; thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCL Oncology ; thema EDItEUR::M Medicine and Nursing::MK Medical specialties, branches of medicine::MKS Medical imaging::MKSH Medical imaging: radiology ; thema EDItEUR::P Mathematics and Science::PH Physics::PHV Applied physics::PHVN Biophysics ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSV Zoology and animal sciences ; thema EDItEUR::R Earth Sciences, Geography, Environment, Planning::RN The environment::RNQ Nuclear issues
    Language: English
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  • 3
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    In Vitro reconstitution and imaging of microtubule dynamics by fluorescence and label-free microscopy (2021)
    Cell Press
    Details
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hirst, W. G., Kiefer, C., Abdosamadi, M. K., Schäffer, E., & Reber, S. In Vitro reconstitution and imaging of microtubule dynamics by fluorescence and label-free microscopy. STAR Protocols, 1(3), (2020): 100177, doi:10.1016/j.xpro.2020.100177.
    Description: Dynamic microtubules are essential for many processes in the lives of eukaryotic cells. To study and understand the mechanisms of microtubule dynamics and regulation, in vitro reconstitution with purified components has proven a vital approach. Imaging microtubule dynamics can be instructive for a given species, isoform composition, or biochemical modification. Here, we describe two methods that visualize microtubule dynamics at high speed and high contrast: (1) total internal reflection fluorescence microscopy and (2) label-free interference reflection microscopy.
    Description: We thank the AMBIO imaging facility (Charité, Berlin) and Nikon at MBL for imaging support. We thank all former and current members of the Reber lab for discussion and helpful advice, in particular Christoph Hentschel and Soma Zsoter for technical assistance. S.R. acknowledges funding by the IRI Life Sciences (Humboldt-Universität zu Berlin, Excellence Initiative/DFG). W.H. was supported by the Alliance Berlin Canberra co-funded by a grant from the Deutsche Forschungsgemeinschaft (DFG) for the International Research Training Group (IRTG) 2290 and the Australian National University. C.K. thanks the Deutsche Forschungsgesellschaft (DFG, JA 2589/1-1). C.K. and M.A. thank Steve Simmert and Tobias Jachowski former and current members of the Schäffer lab.
    Keywords: Biophysics ; Cell Biology ; Microscopy
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 4
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    Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography (2021)
    Cell Press
    Details
    Publication Date: 2022-05-26
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Geisterfer, Z. M., Oakey, J., & Gatlin, J. C. . Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography. STAR Protocols, 1(3), (2020): 100221, doi:10.1016/j.xpro.2020.100221.
    Description: Cell-free extract derived from the eggs of the African clawed frog Xenopus laevis is a well-established model system that has been used historically in bulk aliquots. Here, we describe a microfluidic approach for isolating discrete, biologically relevant volumes of cell-free extract, with more expansive and precise control of extract shape compared with extract-oil emulsions. This approach is useful for investigating the mechanics of intracellular processes affected by cell geometry or cytoplasmic volume, including organelle scaling and positioning mechanisms. For complete details on the use and execution of this protocol, please refer to Geisterfer et al. (2020).
    Description: This work was made possible by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant no. 2P20GM103432. It was also supported by additional funding provided by the NIGMS under grant no. R01GM113028, the NSF Faculty CAREER Program under award no. BBBE 1254608, Whitman Center fellowships at the Marine Biological Laboratory, and the Biomedical Scholars program of the Pew Charitable Trusts. We thank Drs. Aaron Groen and Tim Mitchison for their intellectual contributions and involvement in some of the pioneering experiments that set the foundation for this approach.
    Keywords: Biophysics ; Cell Biology ; Cell isolation ; Microscopy ; Model Organisms
    Repository Name: Woods Hole Open Access Server
    Type: Article
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